CN101387631B - Method for predicting shelf life of UHT milk - Google Patents
Method for predicting shelf life of UHT milk Download PDFInfo
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- CN101387631B CN101387631B CN2008101722550A CN200810172255A CN101387631B CN 101387631 B CN101387631 B CN 101387631B CN 2008101722550 A CN2008101722550 A CN 2008101722550A CN 200810172255 A CN200810172255 A CN 200810172255A CN 101387631 B CN101387631 B CN 101387631B
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Abstract
The invention relates to a method for predicating UHT milk shelf life, comprising the steps of: setting a lower protein particle diameter of UHT milk under a normal temperature T2 as a shelf life reference value, while the storage time and the storage temperature under the shelf life reference value meet an equation: Logt=A/T+M, wherein t means the storage time, T means the storage temperature, A means the characteristic coefficient, M means the correlation coefficient; checking the lower protein particle diameter of UHT milk stored under an actual storage temperature T1, substituting the actual storage temperature T1 and the actual storage time t1 into the equation when the upper lipid particle diameter reaches the shelf life reference value in the actual storage time t1, to calculate A; substituting M, A and T2 into the equation, to calculate the shelf life t2 of the UHT milk under normal temperature. The invention builds a method for using lower protein particle diameter to predicate UHT milk shelf life, which can find the shelf life of a new developed UHT product in short time, to convert developmental achievement into economic benefit quickly.
Description
Technical field
The present invention relates to a kind of method of predicating UHT milk shelf life, especially a kind of method of utilizing the lower floor's albumen particle diameter that detects the UHT Ruzhong to come predicating UHT milk shelf life.
Background technology
UHT (Ultra High Temperature) breast is a kind of product that occurs in the sixties in 20th century, and it is meant the dairy product that the technological process that adopts ultra high temperature sterilization (UHTS) and sterile filling processes.
Compare with Pasteur's breast, the UHT breast has solved the problem of liquid newborn transportation, storage, fresh-keeping difficulty, has kept nutriments such as protein, calcium effectively.Its characteristic is: product can reach commercial sterilization, only needs to preserve at normal temperatures, and distribution does not need cold chain, shelf life long (generally can store 1～6 month), and the nutrition in Ruzhong (except indivedual water soluble vitamins) is identical with pasteurization milk substantially.UHT compares with traditional pasteurize, can significantly reduce the loss of cow's milk color, local flavor and nutritive value, thereby becomes present the best and processing mode with fastest developing speed.
The time that food shelf index futures product can be stored, product quality can be accepted and satisfy the time span of client's quality requirements in period of storage.Shelf life accelerated tests (ASLT, Accelerated shelf-lifetesting) mainly is at the product of long shelf life long inefficient problem of cycle and a kind of experimental technique of growing up under study for action.This experimental technique is product to be placed quicken its reaction under the fierce condition, makes the real shelf life that (is shorter than the time of its real shelf life) in the short period of time and dopes product.The condition that accelerated test is used mainly contains: high temperature, high humidity, illumination, stricter temperature cycles and high concussion etc., use high humidity, high temperature and illumination in food service industry more, wherein the temperature accelerated test use comparatively extensive.
In recent years, businessman is devoted to study the product of long shelf life in order to obtain to sell interests, for example is subjected to shelf life extension (Extended shelf-life ESL) breast, i.e. the ESL breast of domestic and international extensive concern in recent years.The product of research long shelf life has become a kind of development trend.
At present, all do not detect the particularly method of UHT milk shelf life of cow's milk in GB and the industry standard, present judgement for UHT new product shelf life, the main factors such as the comprehensive sterilization mode of research and development person, packaged form that rely on, provide empirical value, afterwards product is placed under normal temperature condition, routine observation is determined the shelf life of product with this.The result that this method is judged has very strong subjectivity, and judges chronicly, needs to verify over 6-8 month the accuracy of judgement usually.The time that research needs in the process of improving the UHT breast and developing new product is long, and drain on manpower and material resources is bigger.
Breast itself is as a buffer system, protein is to be in steady state (SS) in the equilibrium system that various ions are formed, but, cause lactoprotein to be assembled and form precipitation when condition changing (excessive as acidity, superheated, add some salt) may influence the intrinsic salt equilibrium system in Ruzhong.The Ruzhong calcium ion is to the stability influence maximum of breast, because the calcium ion in Ruzhong forms the calcium bridge, thereby lactoprotein is connected into stable long-chain, but when calcium ion is precipitated, just lost its pivotal role, make the lactoprotein micelle become very unstable, finally can precipitate.The cow's milk lay up period, albumen can constantly be assembled, and lower floor's albumen particle diameter can constantly increase, and makes the lactoprotein precipitation, influences product quality.
Summary of the invention
The purpose of this invention is to provide a kind of method,, but judge the shelf life of UHT breast within a short period of time by this method with lower floor albumen particle diameter predicating UHT milk shelf life.
The invention provides a kind of method of predicating UHT milk shelf life, comprise the steps:
1) lower floor's albumen particle size values of setting UHT Ruzhong under the normal temperature T2 is as the shelf life reference value, and period of storage and storage temperature satisfy following equation under the described shelf life reference value:
Logt＝A/T+M
Wherein, t is a period of storage, and T is a storage temperature, and A is a characteristic coefficient, and M is a related coefficient;
2) detect actual storage temperature T1 lower floor's albumen particle diameter in the UHT Ruzhong of storage down, when actual period of storage t1 detects when obtaining lower floor's albumen particle diameter and reaching described shelf life reference value, with described actual storage temperature T1 and the above-mentioned equation of described actual period of storage t1 substitution, try to achieve characteristic coefficient A;
3) with described related coefficient M, characteristic coefficient A and the above-mentioned equation of described normal temperature temperature T 2 substitutions, try to achieve described UHT breast shelf life t2 at normal temperatures.
Among the present invention, above-mentioned shelf life reference value is 0.607-0.698um.Related coefficient M is 0.069-0.076.
Described actual storage temperature T1 is 40-50 ℃, is preferably 45 ℃.Described normal temperature T2 is 25 ℃.
Described step 2) in, lower floor's albumen particle diameter in the UHT Ruzhong that detection in every 3-5 days is once stored.
Get UHT breast lower floor albumen, use laser diffraction particle diameter instrument that the particle diameter of described lower floor albumen is detected.
Get UHT breast lower floor albumen, use laser diffraction particle diameter instrument that the particle diameter of described lower floor albumen is detected.
The parameter that described laser diffraction particle diameter instrument is relevant is as follows: working time: 60s, and revolution speed: 50%, flush time: 8s repeats washing time: 3 times.Clean (needing deionized water), parameter testing when the instrument process, sample can be put into instrument and detect.
The sample injection method of described laser diffraction particle diameter instrument is: carefully take out UHT breast sample, try not to rock, carefully carefully draw the albumen 5-10 μ L of lower floor with liquid-transfering gun, put into the instrument injection port, when PIDS=40-50% appears in instrument, show that the sample size of sample is moderate, detect; If PIDS＜40-50%, expression sample size deficiency, instrument is difficult to it is detected, and need add quantity of sample again, until PIDS=40-50%.
At the cow's milk lay up period, albumen can constantly be assembled, and lower floor's albumen particle diameter can constantly increase, and among the present invention, the end point values of described lower floor albumen particle diameter is 0.607-0.698um.Store the UHT breast down at 40-50 ℃, when its lower floor's albumen particle diameter reached 0.607-0.698um, record period of storage t1, storage temperature T1 set related coefficient M.Only need t1, T1 and M substitution Logt=A/T+M can be obtained the UHT breast shelf life of being stored at normal temperatures.
The present invention detects the UHT milk shelf life with lower floor's albumen particle diameter, when exploitation UHT breast new product, predicts its shelf life in this way within a short period of time, reduces the input of R﹠D cycle and manpower and materials, makes the research and development achievement be converted into economic benefit as early as possible.Method of the present invention can also be used to detecting the shelf life of existing procucts.Method detection speed of the present invention is fast, needs to finish usually in 10-20 days, and generally needs 2-6 month with existing method.Method error of the present invention was less than 10 days, and computing method are simple.
Embodiment
Embodiments of the present invention are intended to explain invention; rather than restriction protection scope of the present invention; the any those of ordinary skill in this area all should be able to know clearly and understand; to specifications disclosed proportional range be can completely inerrably implement of the present invention; any difference slightly on the numerical value that causes because of the difference in cow's milk breast source should be as the different slightly reason of testing result of the present invention; so the present invention is intended to protect the method for coming predicating UHT milk shelf life of utilizing the lower floor's albumen particle diameter that detects the UHT Ruzhong, rather than ratio especially strictly will be limited on the some concrete numerical value.
Embodiment 1: the shelf life of prediction UHT breast
The shelf life reference value of setting lower floor's albumen particle diameter in UHT Ruzhong under the normal temperature (T2=25 ℃) is 0.607-0.698um, and under this shelf life reference value, period of storage and storage temperature satisfy following equation:
Logt＝A/T+M
Wherein, t is a period of storage, and T is a storage temperature, and A is a characteristic coefficient, and M is a related coefficient.
Related coefficient under this reference value is got M=0.073.
From producing, extract one batch UHT breast sample, be stored under 45 ℃ lower floor's albumen particle diameter of the per five days once actual storage temperature of mensuration (T1=45 ℃) storage UHT breasts.
The detection step is as follows:
1, debugging laser diffraction particle diameter instrument (LS13320Beckman Coulter USA), parameter is as follows: working time: 60s, revolution speed: 50%, flush time: 8s repeats washing time: 3 times.Clean (needing deionized water), parameter testing when the instrument process, sample can be put into instrument and detect.
2, carefully take out UHT breast sample, try not to rock, in order to avoid destroy the structural state of sample, carefully cut bag, carefully draw the albumen 5-10 μ L of lower floor, put into the instrument injection port with liquid-transfering gun, when PIDS=40-50% appears in instrument, show that the sample size of sample is moderate, can begin to detect; If PIDS＜40-50%, expression sample size deficiency, instrument is difficult to it is detected, and need add quantity of sample again, until PIDS=40-50%.
When lower floor's albumen particle diameter in the UHT Ruzhong of T1=45 ℃ of storage reaches 0.665um, fallen in the shelf life reference value 0.607-0.698um scope that sets, writing down actual period of storage t1 is 20 days.
With M, T1 and the above-mentioned equation of t1 substitution: i.e. Log20=A/45+0.073, try to achieve characteristic coefficient A=55.26.With M, A and 2=25 ℃ of above-mentioned equation of substitution of normal temperature temperature T, i.e. Logt2=55.26/25+0.073, trying to achieve the shelf life t2 that stores this UHT breast under the normal temperature is 192 days.
This UHT breast is stored down 25 ℃ of normal temperature, detected lower floor's albumen particle diameter in per five days, the period of storage when lower floor's albumen particle diameter reaches above-mentioned shelf life reference value under the record normal temperature is 194 days.The actual shelf life of this UHT breast and above-mentioned predicting the outcome differ 2 days.
Embodiment 2: the shelf life of prediction UHT breast
The shelf life reference value of setting lower floor's albumen particle diameter in UHT Ruzhong under the normal temperature (T2=25 ℃) is 0.607-0.698um, and under this shelf life reference value, period of storage and storage temperature satisfy following equation:
Logt＝A/T+M
Wherein, t is a period of storage, and T is a storage temperature, and A is a characteristic coefficient, and M is a related coefficient.
Related coefficient under this reference value is got M=0.074.
From producing, extract one batch UHT breast sample, be stored under 45 ℃ lower floor's albumen particle diameter of the per three days once actual storage temperature of mensuration (T1=45 ℃) storage UHT breasts.
It is identical with detection step among the embodiment 1 to detect step.
When lower floor's albumen particle diameter in the UHT Ruzhong of T1=45 ℃ of storage reaches 0.653um, fallen in the shelf life reference value 0.607-0.698um scope that sets, writing down actual period of storage t1 is 17 days.
With M, T1 and the above-mentioned equation of t1 substitution: i.e. Log17=A/45+0.074, try to achieve characteristic coefficient A=52.04.With M, A and 2=25 ℃ of above-mentioned equation of substitution of normal temperature temperature T, i.e. Logt2=52.04/25+0.074, trying to achieve the shelf life t2 that stores this UHT breast under the normal temperature is 143 days.
This UHT breast is stored down 25 ℃ of normal temperature, detected lower floor's albumen particle diameter in per three days, the period of storage when lower floor's albumen particle diameter reaches above-mentioned shelf life reference value under the record normal temperature is 140 days.The actual shelf life of this UHT breast and above-mentioned predicting the outcome differ 3 days.
Embodiment 3: the shelf life of prediction UHT breast
The shelf life reference value of setting lower floor's albumen particle diameter in UHT Ruzhong under the normal temperature (T2=25 ℃) is 0.607-0.698um, and under this shelf life reference value, period of storage and storage temperature satisfy following equation:
Logt＝A/T+M
Wherein, t is a period of storage, and T is a storage temperature, and A is a characteristic coefficient, and M is a related coefficient.
Related coefficient under this reference value is got M=0.069.
From producing, extract one batch UHT breast sample, be stored under 45 ℃ lower floor's albumen particle diameter of the per five days once actual storage temperature of mensuration (T1=45 ℃) storage UHT breasts.
It is identical with detection step among the embodiment 1 to detect step.
When lower floor's albumen particle diameter in the UHT Ruzhong of T1=45 ℃ of storage reaches 0.632um, fallen in the shelf life reference value 0.607-0.698um scope that sets, writing down actual period of storage t1 is 16 days.
With M, T1 and the above-mentioned equation of t1 substitution: i.e. Log16=A/45+0.069, try to achieve characteristic coefficient A=51.08.With M, A and 2=25 ℃ of above-mentioned equation of substitution of normal temperature temperature T, i.e. Logt2=51.08/25+0.069, trying to achieve the shelf life t2 that stores this UHT breast under the normal temperature is 129 days.
This UHT breast is stored down 25 ℃ of normal temperature, detected lower floor's albumen particle diameter in per five days, the period of storage when lower floor's albumen particle diameter reaches above-mentioned shelf life reference value under the record normal temperature is 134 days.The actual shelf life of this UHT breast and above-mentioned predicting the outcome differ 5 days.
Embodiment 4: the shelf life of prediction UHT breast
The shelf life reference value of setting lower floor's albumen particle diameter in UHT Ruzhong under the normal temperature (T2=25 ℃) is 0.607-0.698um, and under this shelf life reference value, period of storage and storage temperature satisfy following equation:
Logt＝A/T+M
Wherein, t is a period of storage, and T is a storage temperature, and A is a characteristic coefficient, and M is a related coefficient.
Related coefficient under this reference value is got M=0.076.
From producing, extract one batch UHT breast sample, be stored under 45 ℃ lower floor's albumen particle diameter of the per three days once actual storage temperature of mensuration (T1=45 ℃) storage UHT breasts.
It is identical with detection step among the embodiment 1 to detect step.
When lower floor's albumen particle diameter in the UHT Ruzhong of T1=45 ℃ of storage reaches 0.687um, fallen in the shelf life reference value 0.607-0.698um scope that sets, writing down actual period of storage t1 is 20 days.
With M, T1 and the above-mentioned equation of t1 substitution: i.e. Log20=A/45+0.076, try to achieve characteristic coefficient A=55.13.With M, A and 2=25 ℃ of above-mentioned equation of substitution of normal temperature temperature T, i.e. Logt2=55.13/25+0.076, trying to achieve the shelf life t2 that stores this UHT breast under the normal temperature is 191 days.
This UHT breast is stored down 25 ℃ of normal temperature, detected lower floor's albumen particle diameter in per three days, the period of storage when lower floor's albumen particle diameter reaches above-mentioned shelf life reference value under the record normal temperature is 197 days.The actual shelf life of this UHT breast and above-mentioned predicting the outcome differ 6 days.
Embodiment 5: the shelf life of prediction UHT breast
The shelf life reference value of setting lower floor's albumen particle diameter in UHT Ruzhong under the normal temperature (T2=25 ℃) is 0.607-0.698um, and under this shelf life reference value, period of storage and storage temperature satisfy following equation:
Logt＝A/T+M
Wherein, t is a period of storage, and T is a storage temperature, and A is a characteristic coefficient, and M is a related coefficient.
Related coefficient under this reference value is got M=0.071.
From producing, extract one batch UHT breast sample, be stored under 40 ℃ lower floor's albumen particle diameter of the per three days once actual storage temperature of mensuration (T1=40 ℃) storage UHT breasts.
It is identical with detection step among the embodiment 1 to detect step.
When lower floor's albumen particle diameter in the UHT Ruzhong of T1=40 ℃ of storage reaches 0.653um, fallen in the shelf life reference value 0.607-0.698um scope that sets, writing down actual period of storage t1 is 12 days.
With M, T1 and the above-mentioned equation of t1 substitution: i.e. Log12=A/40+0.071, try to achieve characteristic coefficient A=40.33.With M, A and 2=25 ℃ of above-mentioned equation of substitution of normal temperature temperature T, i.e. Logt2=40.33/25+0.071, trying to achieve the shelf life t2 that stores this UHT breast under the normal temperature is 48 days.
This UHT breast is stored down 25 ℃ of normal temperature, detected lower floor's albumen particle diameter in per three days, the period of storage when lower floor's albumen particle diameter reaches above-mentioned shelf life reference value under the record normal temperature is 46 days.The actual shelf life of this UHT breast and above-mentioned predicting the outcome differ 2 days.
Embodiment 6: the shelf life of prediction UHT breast
The shelf life reference value of setting lower floor's albumen particle diameter in UHT Ruzhong under the normal temperature (T2=25 ℃) is 0.607-0.698um, and under this shelf life reference value, period of storage and storage temperature satisfy following equation:
Logt＝A/T+M
Wherein, t is a period of storage, and T is a storage temperature, and A is a characteristic coefficient, and M is a related coefficient.
Related coefficient under this reference value is got M=0.075.
From producing, extract one batch UHT breast sample, be stored under 50 ℃ lower floor's albumen particle diameter of the per three days once actual storage temperature of mensuration (T1=50 ℃) storage UHT breasts.
It is identical with detection step among the embodiment 1 to detect step.
When lower floor's albumen particle diameter in the UHT Ruzhong of T1=50 ℃ of storage reaches 0.673um, fallen in the shelf life reference value 0.607-0.698um scope that sets, writing down actual period of storage t1 is 14 days.
With M, T1 and the above-mentioned equation of t1 substitution: i.e. Log14=A/50+0.075, try to achieve characteristic coefficient A=53.56.With M, A and 2=25 ℃ of above-mentioned equation of substitution of normal temperature temperature T, i.e. Logt2=53.56/25+0.075, trying to achieve the shelf life t2 that stores this UHT breast under the normal temperature is 165 days.
This UHT breast is stored down 25 ℃ of normal temperature, detected lower floor's albumen particle diameter in per three days, the period of storage when lower floor's albumen particle diameter reaches above-mentioned shelf life reference value under the record normal temperature is 174 days.The actual shelf life of this UHT breast and above-mentioned predicting the outcome differ 9 days.
It should be appreciated by those skilled in the art that per three days described in the above embodiment or per five days detect once, and carrying out according to this time interval of not meaning that whole detections all must strictness.When detecting resulting lower floor albumen particle size values comparatively near the shelf life reference value, assay intervals can shorten, and for example detects once every day, to obtain result accurately.
Detect the method for UHT milk shelf life with lower floor's albumen particle diameter, but determine UHT breast new product shelf life within a short period of time, reduce the input of R﹠D cycle and manpower and materials, make the research and development achievement be converted into economic benefit as early as possible, computing method are simple.
Claims (7)
1. the method for a predicating UHT milk shelf life comprises the steps:
1) lower floor's albumen particle size values of setting UHT Ruzhong under the normal temperature T2 is as the shelf life reference value, and described shelf life reference value is 0.607-0.698 μ m, and period of storage and storage temperature satisfy following equation under the described shelf life reference value:
Logt＝A/T+M
Wherein, t is a period of storage, and unit is day, and T is a storage temperature, and unit is ℃, and A is a characteristic coefficient, and M is a related coefficient, and described related coefficient M is 0.069-0.076;
2) detect actual storage temperature T1 lower floor's albumen particle diameter in the UHT Ruzhong of storage down, described actual storage temperature T1 is 40-50 ℃, when actual period of storage t1 detects when obtaining lower floor's albumen particle diameter and reaching described shelf life reference value, with described actual storage temperature T1 and the above-mentioned equation of described actual period of storage t1 substitution, try to achieve characteristic coefficient A;
3) with described related coefficient M, characteristic coefficient A and the above-mentioned equation of described normal temperature temperature T 2 substitutions, try to achieve described UHT breast shelf life t2 at normal temperatures.
2. the method for claim 1 is characterized in that, described actual storage temperature T1 is 45 ℃.
3. the method for claim 1 is characterized in that, described normal temperature T2 is 25 ℃.
4. the method for claim 1 is characterized in that, described step 2) in, lower floor's albumen particle diameter in the UHT Ruzhong that detection in every 3-5 days is once stored.
5. as the described method of one of claim 1-4, it is characterized in that, get UHT breast lower floor albumen, use laser diffraction particle diameter instrument that the particle diameter of described lower floor albumen is detected.
6. method as claimed in claim 5 is characterized in that, the parameter that described laser diffraction particle diameter instrument is relevant is as follows: working time: 60s, and revolution speed: 50%, flush time: 8s repeats washing time: 3 times.
7. method as claimed in claim 6 is characterized in that, the sample injection method of described laser diffraction particle diameter instrument is: the UHT breast of described storage can not rock, and draws the described albumen 2-5 μ L of lower floor, puts into the injection port of described laser diffraction particle diameter instrument, detects.
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| CN102207460B (en) * | 2010-03-30 | 2012-10-03 | 光明乳业股份有限公司 | Method for detecting shelf life of ultra heat treated milk |
| CN101846666A (en) * | 2010-05-07 | 2010-09-29 | 内蒙古伊利实业集团股份有限公司 | Method for calculating shelf life of UHT pure milk and method for calculating content of upper protein of UHT pure milk |
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| US5824494A (en) * | 1994-05-01 | 1998-10-20 | Sirotech Ltd. | Method for enumerating bacterial populations |
| CN101142838A (en) * | 2005-01-19 | 2008-03-12 | 新加坡电信有限公司 | System for routing wireless communication |
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| US5824494A (en) * | 1994-05-01 | 1998-10-20 | Sirotech Ltd. | Method for enumerating bacterial populations |
| CN101142838A (en) * | 2005-01-19 | 2008-03-12 | 新加坡电信有限公司 | System for routing wireless communication |
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