CN101948744A - Cell vaccinating device of lumen-shaped matrix, vaccinating cavity and vaccinating method - Google Patents
Cell vaccinating device of lumen-shaped matrix, vaccinating cavity and vaccinating method Download PDFInfo
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- CN101948744A CN101948744A CN2010102587731A CN201010258773A CN101948744A CN 101948744 A CN101948744 A CN 101948744A CN 2010102587731 A CN2010102587731 A CN 2010102587731A CN 201010258773 A CN201010258773 A CN 201010258773A CN 101948744 A CN101948744 A CN 101948744A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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Abstract
The invention provides a vaccinating cavity which has the advantages of simplicity and low cost and can rotate and be used for carrying out uniform cell vaccination on a lumen-shaped matrix, a vaccinating device and a vaccinating method, which can be used for carrying out uniform cell vaccination on inner cavities of lumen-shaped matrixes with different lengths and diameters. According to an embodiment of the invention, the cell vaccinating device comprises a rotating motor and a vaccinating cavity 10, wherein the vaccinating cavity consists of a sleeve 12 and sealing plugs 15 and 16; the sleeve consists of a hard pipe 14 and a flexible pipe 13 with elasticity; the hard pipe and the flexible pipe are connected through a thread; the length of the sleeve can be changed through rotating the thread so as to adapt to the lengths of different lumen-shaped matrixes; the lumen-shaped matrix is fixed on joints 17 and 18; and the joints are fixed on the sealing plugs so as to achieve the purposes of fixing a blood vessel and maintaining the sealing and sterile state of the vaccinating cavity.
Description
Technical field
The present invention relates to cell cultures, field of tissue engineering technology, relate more specifically to a kind of rotatable classification inoculation apparatus, inoculation chamber and inoculation method that can carry out even cell inoculation tube chamber shape matrix.
Background technology
With Method of Tissue Engineering external structure anatomic implants is high density seed cell with vitro culture, is inoculated on the tissue engineering bracket of natural or synthetic, after vitro culture, is transplanted in the body, to substitute the sick tissue that decreases again.Make up the tissue that arteries, oesophagus, tracheae etc. have tube chamber, it is first key link that engineering tissue makes up that cell is evenly inoculated on the tubulose three-dimensional rack, the even inoculation of cell helps between the cell, set up between cell and the cell matrix and get in touch, increasing growth and the product of cell on support forms, shorten and organize formation time, improve tissue and form quality; Always form quality and interrelate and cell inoculation is inhomogeneous with relatively poor tissue.
Inoculating cell at the tubular bracket inner chamber mainly contains static inoculation and two kinds of dynamic inoculation techniques at present.Static inoculation is meant that seed cell relies on action of gravity attached on the support.Static inoculation is simple to operate, be inoculation method the most frequently used in the organizational project, but the inoculation uniformity coefficient of cell in tubular bracket is poor, has only the bottom inoculation of tube chamber to have cell, other parts not to have cell to attach.Dynamically inoculation has rotation (rotating seeding), shakes inoculation modes such as (agitated seeding); Dynamically inoculation has improved inoculation efficient and the seed cell inoculation uniformity coefficient at rack surface, but still exists inoculation efficient low, and the rotation inoculation is to the equipment requirements height on reactor, and inoculum is difficult for problems such as taking-up.
Chinese invention patent ZL200480023921 provides a kind of cell inoculation device to the even inoculating cell of matrix that three-dimensional surface is arranged, adopts the rotation inoculum that two turning axles are arranged, and makes the cell of inoculation be evenly distributed on rack surface; This device need mix rotation with seed liquor with inoculum in inoculation container, the tube chamber surfaces externally and internally has all been inoculated cell, can not be at tube chamber internal surface inoculating cell; And need be for blood vessel etc. at the timbering material of tube chamber internal surface inoculating cell, under slow speed of revolution condition, tube chamber inner transmission matter weak effect, intravascular stent can not be fixed, and are unfavorable for even inoculation.In addition, the size of the lumen organization of inoculation also is subjected to the inoculation container limitation of size.
Soletti etc. provide a kind of rotation classification inoculation apparatus (Soletti L that is applicable to vascular tissue, Nieponice A, Guan J, Stankus JJ, Wagner WR, Vorp DA.2006.A seeding device for tissue engineered tubular structures.Biomaterials27 (28): 4863-4870.), intravascular stent is fixed in the inoculation chamber, pump into seed liquor with syringe pump, vacuumize the inoculation of realization high-density with vacuum pump, make the cell uniform distribution by the rotation intravascular stent.This appliance arrangement complexity, the operation easier height, the long a large amount of wastes that cause seed cell of pipeline, inoculation efficient is low, and inoculum is not easy to take out, and uses the easy pair cell of vacuum take-off nutrient solution to damage.
Summary of the invention
The purpose of this invention is to provide a kind of device is simple, cost is low, can rotate, can carry out tube chamber shape matrix (support) inner chamber even cell inoculation inoculation chamber, classification inoculation apparatus and inoculation method, its characteristics are 1, the inoculation chamber is made up of the sleeve pipe that one section hard tube and one section elastic hose link together, the length in inoculation chamber can be regulated, by fixing elastic hose carry out inoculum fixing, take out and inoculation is operated; There is fixedly tube chamber shape matrix of joint and sealing plug at 2 inoculation two ends, chamber, prevent matrix distortion winding.3, tube chamber shape matrix is inoculated in the inoculation chamber, and take out the inoculation back, easy to operate, inoculation efficient height; 4, the inoculation chamber is connected to rotating machine, and the rotation of inoculation chamber makes inoculating cell at tube chamber shape matrix internal surface uniform distribution; Classification inoculation apparatus provided by the invention and inoculation method can be used for the inoculation culture of through engineering approaches tube chamber shape tissue (as blood vessel, oesophagus, tracheae, the bladder etc.) inner cell of field of tissue engineering technology.
Adopt classification inoculation apparatus of the present invention to realize the tubulose matrix inner chamber of different lengths and diameter is carried out uniform cell inoculation.
According to an aspect of the present invention, provide a kind of tube chamber shape inoculated stromal cell chamber, it is characterized in that comprising:
A sleeve pipe, described sleeve pipe comprise one section hard tube and one section resilient flexible pipe, and described hard tube and described flexible pipe be by being threaded,
Hard tube end sealing plug is used to seal the free end of described hard tube,
The hose end sealing plug is used to seal the free end of described flexible pipe.
According to another aspect of the present invention, provide a kind of tube chamber shape inoculated stromal cell device, it is characterized in that comprising:
A sleeve pipe, described sleeve pipe comprise one section hard tube and one section resilient flexible pipe, and described hard tube and described flexible pipe be by being threaded,
Hard tube end sealing plug is used to seal the free end of described hard tube,
The hose end sealing plug is used to seal the free end of described flexible pipe,
The coupling that rotates of a rotating machine, its output shaft and described inoculation chamber.
Another aspect of the present invention provides a kind of cell inoculation method to set up of tube chamber shape matrix, it is characterized in that comprising:
The two ends of tube chamber shape matrix are connected respectively on hose end joint and the hard tube end connector,
Described hard tube end connector is fixed on the hard tube end sealing plug,
The described hard tube end sealing plug that is connected with described tube chamber shape matrix is connected on the free end of hard tube in a cell inoculation chamber, thereby the free end that seals described hard tube is airtight, wherein said hard tube constitutes a sleeve pipe with a resilient flexible pipe by being threaded
Regulate being threaded between described flexible pipe and the hard tube, thereby regulate described telescopic length, adapting to the length of described tube chamber shape matrix,
The free end of clamping described flexible pipe to be securing the above the hose end joint,
In described tube chamber shape matrix, inject seed cell,
The described free end of clamping described flexible pipe to be securing the above the hose end joint,
With described hose end sealing plug plug to the described free end of described flexible pipe, thereby the hose end in airtight described cell inoculation chamber is connected to described hose end joint on the described hose end sealing plug simultaneously, thereby the hose end of described tube chamber shape matrix is fixed.
Description of drawings
Fig. 1 has illustrated to show the structure of rotatable cell inoculation device to tube chamber shape matrix according to an embodiment of the invention.
Fig. 2 has illustrated to show the structure in cell inoculation according to an embodiment of the invention chamber.
Fig. 3 a-3f has shown utilization classification inoculation apparatus cell according to the present invention behind the intravascular stent inoculating cell of glass material, the distribution situation of the cell of examining under a microscope on periphery of rack bore.Demonstrate cell uniform distribution on the various piece of a periphery of rack bore.
Fig. 4 a and 4b shown and utilize classification inoculation apparatus cell provided by the invention behind the intravascular stent inoculating cell of acellular matrix, and the support of inoculation is carried out the observed cell in HE dyeing back in the distribution situation of cell on periphery of rack bore.Demonstrate cell uniform distribution on periphery of rack bore.
Embodiment
As shown in Figure 1, cell inoculation device according to an embodiment of the invention comprises a rotating machine 11 and an inoculation chamber 10, wherein inoculates the chamber and comprises sleeve pipe 12 and sealing plug 15,16.Sleeve pipe comprises one section hard tube 14 and one section resilient flexible pipe 13 (see figure 2).Hose end sealing plug 15 is used for the free end of airtight flexible pipe 13.Hard tube end sealing plug 16 is used for the free end of airtight hard tube 14.
According to a specific embodiment, the output shaft 20 of rotating machine 11 is by the coupling that rotates of gear unit 21 and inoculation chamber 10, thereby can drive 10 rotations of inoculation chamber.
It will be appreciated that the output shaft of rotating machine 11 20 is not limited to gear unit with the rotation coupled modes in inoculation chamber 10, also can be belt transmission, chain gear etc., these all be those skilled in the art can understand.
According to a specific embodiment, hard tube 14 and flexible pipe 13 are by being threaded; Like this, the length of sleeve pipe 12 can change by the rotation screw thread, thereby can adapt to the length of different vaccination thing.It will be appreciated that the mode of connection of hard tube 14 and flexible pipe 13 is not limited to be threaded, also can be that an end of hard tube inserts in the end of flexible pipe, connects hard tube etc. by the elasticity of flexible pipe, these all be those skilled in the art can understand.
According to a specific embodiment, hard tube 14 and flexible pipe 13 can be cylindrical tubes, also can be chamber in irregular shape shape pipes, to adapt to the shape of tube chamber shape matrix to be inoculated.
Tube chamber shape matrix (support) 19 to be cultivated is fixed on the joint 17,18.Joint 17,18 is fixed on respectively on the sealing plug 15,16, thereby reaches the fixedly airtight aseptic state of tube chamber shape matrix and maintenance inoculation chamber.
The tubing of this tube chamber shape matrix comprises:
-through taking off the animal tube chamber shape organization bracket that cell is handled, as the blood vessel of acellular matrix, oesophagus, trachea bracket etc.
-the tubing that constitutes by natural materialss such as collagen, silk fiber, wool fibers
-the tubing that constitutes by superpolymer such as PLGA, PLA, PLG, sodium alginate, tetrafluoroethylene,
The tubing of above-mentioned tube chamber shape matrix is as the tissue engineering bracket of the tube chamber shape histoorgan that makes up humans and animals
According to one embodiment of present invention, the joint 17,18 that has installed support to be cultivated 19 is separately fixed on the sealing plug 15,16.Inoculation chamber and rotating machine 11 are connect, realize that by rotation tube chamber shape matrix 19 seed cell is at the even inoculation of tube chamber shape matrix 19 inwalls and the even mass transfer of nutrient solution, the design of tube chamber shape matrix 19 two ends fixed guarantees softish tube chamber shape matrix 19 winding that can not be distorted in rotary course, because the elasticity of flexible pipe 13, guaranteed in seeded process can (by instrument) such as mosquito forceps fixing softish tube chamber shape matrix, guarantee inoculation operation and inoculation before and after tube chamber shape matrix put into and take out conveniently carrying out of operation.According to one embodiment of present invention, the sealing with O type circle 22 guarantees between sealing plug and the hard tube/flexible pipe can not be flowed out the liquid in the tube chamber shape matrix.
According to one embodiment of present invention, O type circle 22 a close encapsulation plug and hard pipe joint are arranged on the hard tube end connector 18, thereby stop the liquid in the tube chamber shape matrix between described sealing plug and hard pipe joint, to flow out; O type circle 22 a close encapsulation plug and hose nipples are arranged on the hose end joint 17, thereby stop the liquid in the tube chamber shape matrix between hose end sealing plug and hose nipple, to flow out,
According to a specific embodiment, when concrete operations, the two ends with support 19 are connected respectively on the joint 17,18 earlier, and wherein the joint 18 of hard tube end is fixed on the corresponding sealing plug 16 again;
Then, the sealing plug 16 that is connected with support 19 is connected on the hard tube 14 in cell inoculation chamber, makes the hard tube end in cell inoculation chamber airtight;
Subsequently, regulate being threaded between flexible pipe 13 and the hard tube 14, regulate the length of sleeve pipe 12, to adapt to the length of support 19;
Use the joint 17 of end of clamping elastic hose 13 such as the instrument of mosquito forceps, in support 19, inject seed cell liquid with syringe to fix hose end;
Use the joint 17 of end of clamping elastic hose 13 such as the instrument of mosquito forceps to fix hose end, hose end sealing plug 15 is filled on the end of the flexible casing 13 of inoculating the chamber, make the hose end in cell inoculation chamber airtight, simultaneously hose end joint 17 is connected on the sealing plug 15, thereby the hose end of support 19 is fixed;
So, finished the inoculation of support 19 in the cell inoculation device.
The cell inoculation chamber that support has been got well in inoculation is set on the rotating machine, and is placed in the cell culture incubator, opens rotating machine, with predetermined rotating speed rotation preset time.
After inoculation is finished, the cell inoculation chamber is taken off from rotating machine, use such as the instrument of mosquito forceps and clamp the elastic hose end to fix hose end joint 17, sealing plug 15 and joint 17 are separated, sealing plug 15 is taken off from the elastic hose end, again hard tube end sealing plug 16 is taken off from the hard tube end, thereby the good support 19 of inoculation that still is attached on the hard tube end sealing plug 16 can be taken out from the hard tube end.
The support 19 of so having inoculated cell can be for cultivating.
It should be noted that classification inoculation apparatus provided by the invention can inoculate tissue engineering bracket beginning of cultivating, also can in culturing process, repeatedly inoculate tissue engineering bracket.
Described cell comprises that structure is present in various noble cellss in adult and the interior tube chamber shape tissue of animal body, the organ and stem cell such as vascular endothelial cell, vascular smooth muscle cell, mescenchymal stem cell, fat stem cell etc., and cell line cell.
Fig. 3 a-3f has shown that utilization classification inoculation apparatus cell according to the present invention is behind the intravascular stent inoculating cell of glass material, the distribution situation of the cell of examining under a microscope on periphery of rack bore: take a photo for 60 ° in the every rotation of microscopically, Fig. 3 a-f represents 360 ° of (6 times) captured photos of one section tubular bracket rotation.Photo demonstrates, and cell has been realized uniform distribution on the various piece of a periphery of rack bore.
Fig. 4 a and 4b shown and utilize classification inoculation apparatus cell provided by the invention behind the intravascular stent inoculating cell of acellular matrix, and the support of inoculation is carried out the distribution situation of the HE dyeing observed cell in back on periphery of rack bore.Photo demonstrates cell uniform distribution on periphery of rack bore.
Embodiment 1
1. the parts in cleaning and sterilizing cell inoculation chamber;
2. the two ends with intravascular stent (sterile glass tube) are connected on the joint, one of them joint are fixed on the hard tube end sealing plug 16 again;
3. the hard tube end sealing plug 16 that will be connected with intravascular stent is connected on the hard tube in cell inoculation chamber, makes the hard tube end in cell inoculation chamber airtight;
4. regulate being threaded between elastic hose and the hard tube, the regulating sleeve length of tube is to adapt to the length of intravascular stent;
5. clamp the elastic hose end to fix the hose end joint, the usefulness syringe injects the vascular endothelial cell as seed cell liquid in intravascular stent;
6. clamp the elastic hose end to fix the hose end joint, hose end sealing plug 15 is connected on the elastic hose in inoculation chamber, make the hose end in cell inoculation chamber airtight, simultaneously joint is connected on the hose end sealing plug 15, thereby the hose end of intravascular stent is fixed;
7. the cell inoculation chamber is fixed on the rotating machine, classification inoculation apparatus is put into cell culture incubator, open rotating machine, rotating speed 5 commentaries on classics/hour, rotated 2 hours;
8. the cell inoculation chamber is taken off from rotating machine, clamp the elastic hose end to fix the hose end joint, sealing plug 15 and hose end joint are separated, and hose end sealing plug 15 taken off from the elastic hose end, again sealing plug 16 is taken off from the hard tube end, take out from the hard tube end thereby will inoculate good intravascular stent.
The intravascular stent that inoculation is good can be cultivated.
Embodiment 2
1. the parts in cleaning and sterilizing cell inoculation chamber;
2. the two ends with intravascular stent (acellular matrix) are connected respectively on the joint, one of them joint are fixed on the hard tube end sealing plug 16 again;
3. the sealing plug 16 that will be connected with intravascular stent is connected on the hard tube end in cell inoculation chamber, makes the hard tube end in cell inoculation chamber airtight;
4. the spiral of regulating elastic hose 13 and hard tube 14 is connected, thereby the regulating sleeve length of tube is to adapt to the length of intravascular stent;
5. clamp the elastic hose end to fix the hose end joint, the usefulness syringe injects the vascular smooth muscle cell as seed cell liquid in intravascular stent;
6. clamp the elastic hose end to fix the hose end joint, hose end sealing plug 15 is connected on the elastic hose in inoculation chamber, make the hose end in cell inoculation chamber airtight, simultaneously joint is connected on the hose end sealing plug 15, thereby the hose end of intravascular stent is fixed;
7. the cell inoculation chamber is fixed on the rotating machine, classification inoculation apparatus is put into cell culture incubator, open rotating machine, rotating speed 10 commentaries on classics/hour, rotated 2-6 hour;
8. the cell inoculation chamber is taken off from rotating machine, clamp the elastic hose end to fix the hose end joint, hose end sealing plug 15 and hose end joint are separated, and sealing plug 15 taken off from the elastic hose end, again hard tube end sealing plug 16 is taken off from the hard tube end, take out from the hard tube end thereby will inoculate good intravascular stent.
9. inoculated the intravascular stent of vascular smooth muscle cell and cultivated, taken off after waiting to reach requirement, repeated 2-7 step inoculation vascular endothelial cell, will inoculate good intravascular stent again and take out, continued cultivation from the hard tube end.
Embodiment 3
1. the parts in cleaning and sterilizing cell inoculation chamber;
2. the two ends with Esophageal Stent (acellular matrix) are connected on the joint, one of them joint are fixed on the hard tube end sealing plug 16 again;
3. the sealing plug 16 that will be connected with Esophageal Stent is connected on the hard tube end in cell inoculation chamber, makes the hard tube end in cell inoculation chamber airtight;
4. regulate being threaded between elastic hose and the hard tube, thereby the regulating sleeve length of tube is to adapt to the length of Esophageal Stent;
5. clamp the elastic hose end to fix the hose end joint, in Esophageal Stent, inject seed cell liquid with syringe;
6. clamp the elastic hose end to fix the hose end joint, hose end sealing plug 15 is connected on the elastic hose in inoculation chamber, make the hose end in cell inoculation chamber airtight, simultaneously the hose end joint is connected on the hose end sealing plug 15, thereby the hose end of Esophageal Stent is fixed;
7. the cell inoculation chamber is fixed on the rotating machine, classification inoculation apparatus is put into cell culture incubator, open rotating machine, rotating speed 12 commentaries on classics/hour, rotated 2-6 hour;
8. the cell inoculation chamber is taken off from rotating machine, clamp the elastic hose end to fix the hose end joint, hose end sealing plug 15 and hose end joint are separated, and hose end sealing plug 15 taken off from the elastic hose end, again hard tube end sealing plug 16 is taken off from the hard tube end, take out from the hard tube end thereby will inoculate good Esophageal Stent.
The Esophageal Stent that inoculation is good can be cultivated.
Should be understood that, in above narration and explanation to just explanation but not determinate of description that the present invention carried out, and do not breaking away under the prerequisite of the present invention that limits as appended claims, can carry out various changes, distortion and/or correction the foregoing description.
Claims (8)
1. tube chamber shape inoculated stromal cell chamber is characterized in that comprising:
A sleeve pipe (12), described sleeve pipe comprise one section hard tube (14) and one section resilient flexible pipe (13), and an end of an end of described hard tube (14) and described flexible pipe (13) interconnects,
Hard tube end sealing plug (16) is used to seal the free end of described hard tube,
Hose end sealing plug (15) is used to seal the free end of described flexible pipe.
2. tube chamber shape inoculated stromal cell according to claim 1 chamber is characterized in that further comprising:
Be connected to the hose end joint (17) on the described hose end sealing plug (15), be used to install tube chamber shape matrix (19) to be cultivated,
Be connected to the hard tube end connector (18) on the described hard tube end sealing plug (16), be used to install tube chamber shape matrix (19) to be cultivated.
3. tube chamber shape inoculated stromal cell device is characterized in that comprising:
A sleeve pipe (12), described sleeve pipe comprise one section hard tube (14) and one section resilient flexible pipe (13), and described hard tube (14) and described flexible pipe (13) be by being threaded,
Hard tube end sealing plug (16) is used to seal the free end of described hard tube,
Hose end sealing plug (15) is used to seal the free end of described flexible pipe,
The coupling that rotates of a rotating machine (11), its output shaft (20) and described inoculation chamber (10).
4. tube chamber shape inoculated stromal cell device according to claim 3 is characterized in that further comprising:
Be connected to the hose end joint (17) on the described hose end sealing plug (15), be used to install tube chamber shape matrix (19) to be cultivated,
Be arranged on the O type circle (22) on the described hose end joint (17), the liquid that is used for stoping described tube chamber shape matrix (19) is from flowing out between described hose end sealing plug (15) and the described hose end joint (17),
Be connected to the hard tube end connector (18) on the described hard tube end sealing plug (16), be used to install described tube chamber shape matrix (19) to be cultivated,
Be arranged on the O type circle (22) on the described hard tube end connector (18), be used for stoping the liquid of described tube chamber shape matrix from flowing out between described hard tube end sealing plug (16) and the described hose end joint (18).
5. tube chamber shape inoculated stromal cell according to claim 1 chamber is characterized in that
One end of one end of described hard tube (14) and described flexible pipe (13) is by being threaded.
6. tube chamber shape inoculated stromal cell according to claim 1 chamber is characterized in that
One end of described hard tube inserts in the end of described flexible pipe, leans on the elasticity of described flexible pipe that described flexible pipe is connected with described hard tube.
7. the cell inoculation method to set up of tube chamber shape matrix is characterized in that comprising:
The two ends of tube chamber shape matrix (19) are connected respectively on hose end joint (17) and the hard tube end connector (18),
Described hard tube end connector (18) is fixed on the hard tube end sealing plug (16),
The described hard tube end sealing plug (16) that will be connected with described tube chamber shape matrix (19) is connected on the free end of hard tube (14) in a cell inoculation chamber (10), thereby the free end that seals described hard tube is airtight, wherein said hard tube (14) constitutes a sleeve pipe (12) with a resilient flexible pipe (13) by being threaded
Regulate being threaded between described flexible pipe (13) and the hard tube (14), thus the length of regulating described sleeve pipe (12), adapting to the length of described tube chamber shape matrix (19),
The free end of clamping described flexible pipe (13) to be securing the above hose end joint (17),
In described tube chamber shape matrix (19), inject seed cell,
The described free end of clamping described flexible pipe (13) to be securing the above hose end joint (17),
Described hose end sealing plug (15) is filled on the described free end of described flexible pipe (13), thereby the hose end in airtight described cell inoculation chamber, simultaneously described hose end joint (17) is connected on the described hose end sealing plug (15), thereby the hose end of described tube chamber shape matrix (19) is fixed.
8. according to the cell inoculation method to set up of the described tube chamber shape of claim 7 matrix, it is characterized in that further comprising:
The cell inoculation chamber that has set described tube chamber shape matrix (19) is rotated preset time with predetermined rotating speed,
The described free end of clamping described flexible pipe (13) to be securing the above hose end joint (17),
Described hose end sealing plug (15) and hose end joint (17) are separated,
The free end of described hose end sealing plug (15) from described flexible pipe taken off,
The free end of hard tube end sealing plug (16) from described hard tube taken off, thereby the described tube chamber shape matrix (19) that will be contained on the described hard tube end sealing plug (16) is taken out from the free end of hard tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010258773 CN101948744B (en) | 2010-08-20 | 2010-08-20 | Cell vaccinating device of lumen-shaped matrix, vaccinating cavity and vaccinating method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010258773 CN101948744B (en) | 2010-08-20 | 2010-08-20 | Cell vaccinating device of lumen-shaped matrix, vaccinating cavity and vaccinating method |
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| Publication Number | Publication Date |
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| CN101948744A true CN101948744A (en) | 2011-01-19 |
| CN101948744B CN101948744B (en) | 2013-10-23 |
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| CN 201010258773 Expired - Fee Related CN101948744B (en) | 2010-08-20 | 2010-08-20 | Cell vaccinating device of lumen-shaped matrix, vaccinating cavity and vaccinating method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104711187A (en) * | 2015-03-25 | 2015-06-17 | 新奥科技发展有限公司 | Cell inoculation device and inoculation method using same |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5026650A (en) * | 1988-06-30 | 1991-06-25 | The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration | Horizontally rotated cell culture system with a coaxial tubular oxygenator |
| US20060075963A1 (en) * | 2004-10-05 | 2006-04-13 | Alejandro Nieponice | Vacuum rotational seeding and loading device and method for same |
| CN101560469A (en) * | 2008-04-14 | 2009-10-21 | 上海交通大学医学院附属上海儿童医学中心 | Rotary dual-chamber stem cell synchronous augmentation and division inoculator |
| US7759120B2 (en) * | 2005-03-02 | 2010-07-20 | Kps Bay Medical, Inc. | Seeding implantable medical devices with cells |
-
2010
- 2010-08-20 CN CN 201010258773 patent/CN101948744B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5026650A (en) * | 1988-06-30 | 1991-06-25 | The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration | Horizontally rotated cell culture system with a coaxial tubular oxygenator |
| US20060075963A1 (en) * | 2004-10-05 | 2006-04-13 | Alejandro Nieponice | Vacuum rotational seeding and loading device and method for same |
| US7759120B2 (en) * | 2005-03-02 | 2010-07-20 | Kps Bay Medical, Inc. | Seeding implantable medical devices with cells |
| CN101560469A (en) * | 2008-04-14 | 2009-10-21 | 上海交通大学医学院附属上海儿童医学中心 | Rotary dual-chamber stem cell synchronous augmentation and division inoculator |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104711187A (en) * | 2015-03-25 | 2015-06-17 | 新奥科技发展有限公司 | Cell inoculation device and inoculation method using same |
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| CN101948744B (en) | 2013-10-23 |
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