CN102525569A - Array type microdialysis probe - Google Patents
Array type microdialysis probe Download PDFInfo
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- CN102525569A CN102525569A CN2011104446179A CN201110444617A CN102525569A CN 102525569 A CN102525569 A CN 102525569A CN 2011104446179 A CN2011104446179 A CN 2011104446179A CN 201110444617 A CN201110444617 A CN 201110444617A CN 102525569 A CN102525569 A CN 102525569A
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Abstract
The invention discloses an array type microdialysis probe. The array type microdialysis probe comprises a liquid inlet protecting tube, a liquid inlet sealing column, a liquid outlet sealing column, a liquid outlet protecting tube, sealing epoxy resin, a base seat and a plurality of microdialysis probes, wherein the microdialysis probe comprises a high molecular hollow fiber membrane, a stent catheter, a liquid inlet capillary and a liquid outlet capillary. The high molecular hollow fiber membrane and the stent catheter are fixedly connected by the epoxy resin. The high molecular hollow fiber membrane is communicated with the inner cavity of the stent catheter. The base seat is provided with a plurality of through holes. A microdialysis probe is fixed in each through hole of the base seat. The liquid inlet protecting tube is sealed by the liquid inlet sealing column. The liquid outlet sealing column is sealed by the liquid outlet protecting tube. The microdialysis probes are connected in series, in parallel or grouping manner. The probe array disclosed by the invention can be used in a series or parallel manner, so that the effective dialysis area is enlarged and the recovery of organism living body fluid sampling is improved.
Description
Technical field
The present invention relates to a kind of zoopery equipment, relate in particular to a kind of array micro-dialysis device.
Background technology
Micro-dialysis device is can pass through semipermeable membrane with micromolecule and water, along Concentraton gradient spread and macromolecular substances can not to see through semipermeable membrane be a kind of chemical substance sampling apparatus that the basis is designed.When the tubulose dialyzer of minor diameter being implanted in the animal body behind the specific region; With artificial dialysis solution continous perfusion; Then the small-molecule substance in the outer liquid of histiocyte forms Concentraton gradient in the semipermeable membrane both sides, and diffuses into dialysis solution along Concentraton gradient, and is taken out of external by dialysis solution; Through analysis, can calculate the concentration level of this material in the outer liquid of in-vivo tissue to content of material in the dialysis solution.This technology is applied to monitor the research of interior chemical micro-environment variation of body and drug mechanism.
The key parameter of micro-dialysis device is the response rate of material, the ratio of material concentration in material concentration and the probe environmental liquids in the dialysis solution that promptly reclaims.Relating to parameters such as this parameter and target substance molecular weight size, dialyzer material and dialyzer length, ambient temperature, perfusion flow velocity.For predetermined substance and certain ambient temperature, in order to obtain the higher probe response rate, generally can adopt increases dialyzer length and the method that reduces the perfusion flow velocity.For little laboratory animal such as rat, mice etc.; Physical dimension is less in vertical direction for its part all brain structures such as hypothalamus etc.; The effective length of the probe film that can hold is limited; Therefore probe is lower to the response rate of endogenous neurotransmitter or ectogenic pharmacological active substance, influences the detectability that dialysis solution is analyzed.Reduce rate of flooding as passing through, though can improve the response rate of probe, but can reduce temporal resolution, unfavorable to dynamic analysis.Existing microdialysis probe pedestal has certain volume, and it is limited to be limited to laboratory animal head volume, therefore is merely able to examine in the group 1 ~ 3 spacing different cerebral far away respectively simultaneously implant microdialysis probe, carries out the synchronous microdialysis that a plurality of nuclears are rolled into a ball.
Summary of the invention
The objective of the invention is deficiency, a kind of array microdialysis probe is provided to prior art.
The objective of the invention is to realize through following technical scheme: a kind of array microdialysis probe, it comprises: go into the liquid protecting tube, go into the liquid sealing post, fluid sealing column, fluid protecting tube, sealing epoxy resin, pedestal and several microdialysis probes; Wherein, microdialysis probe comprises: hollow fiber polymer membrane, scaffold tube, go into liquid capillary tube and fluid capillary tube, hollow fiber polymer membrane is fixedly connected through epoxy resin with scaffold tube, and the inner chamber of hollow fiber polymer membrane and scaffold tube communicates; Pedestal has some through holes, and microdialysis probe of each through hole internal fixation of pedestal is gone into the liquid protecting tube by going into the liquid sealing column sealing, and the fluid sealing column forms the annexation of series, parallel or grouping by the sealing of fluid protecting tube between each microdialysis probe.
Further; Said placed in-line annexation is specially: also comprise some connection protecting tubes that are used to connect microdialysis probe; Go into liquid protecting tube and fluid protecting tube and be one, the liquid capillary tube of going into of first microdialysis probe passes into the liquid protecting tube, after the fluid capillary tube of first microdialysis probe connects protecting tube through first; Communicate with the liquid capillary tube of going into of second microdialysis probe; The fluid capillary tube of second microdialysis probe communicates with the liquid capillary tube of going into of the 3rd microdialysis probe after connecting protecting tube through second, and the like; The fluid capillary tube of last microdialysis probe passes the fluid protecting tube, and the junction of going between liquid protecting tube, microdialysis probe, connection protecting tube and the fluid protecting tube is connected with epoxy resin by connecting tube.Going into liquid protecting tube, connection protecting tube and fluid protecting tube is sealed and is fixed on the pedestal by sealing epoxy resin.
Further, the annexation of said parallel connection is specially: go into liquid protecting tube and fluid protecting tube and be one, the liquid capillary tube of going into of each microdialysis probe passes respectively into the liquid protecting tube, and the fluid capillary tube of each microdialysis probe passes same fluid protecting tube; The junction of going between liquid protecting tube, microdialysis probe and the fluid protecting tube is connected with epoxy resin by connecting tube; Going into liquid protecting tube and fluid protecting tube is sealed and is fixed on the pedestal by sealing epoxy resin.
Further; The annexation of said grouping is specially: the number of going into liquid protecting tube and fluid protecting tube is N, and microdialysis probe is divided into the N group, and each length of organizing microdialysis probe is different; The length of microdialysis probe is identical on the same group; The number of every group of microdialysis probe is 1 or greater than 1, and when the number of every group of microdialysis probe is greater than 1 the time, each microdialysis probe forms the annexation of serial or parallel connection.
The invention has the beneficial effects as follows that the present invention adapts to the shape and the spatial restriction of cerebral tissue structure, increase effectively dialysis area, improve the response rate of material sampling in the small brain structure.
Description of drawings
Fig. 1 is the embodiment of the invention 1 equidistant, an isometric tandem array microdialysis probe structural representation;
Fig. 2 is the not isometric tandem array microdialysis probe structural representations of the embodiment of the invention 2 groupings;
Fig. 3 is the equidistantly not isometric parallel array microdialysis probe structural representation of the embodiment of the invention 3;
Among the figure, microdialysis probe 1, hollow fiber polymer membrane 2, epoxy resin 3, scaffold tube 4, go into liquid capillary tube 5, fluid capillary tube 6, through hole 7, connecting tube with epoxy resin 8, go into liquid protecting tube 9, go into liquid sealing post 10, connect protecting tube 11, fluid sealing column 12, fluid protecting tube 13, sealing epoxy resin 14, pedestal 15.
The specific embodiment
Describe the present invention in detail according to accompanying drawing and embodiment below, it is more obvious that the object of the invention and effect will become.
Embodiment 1
As shown in Figure 1, the array microdialysis probe of this embodiment comprises: go into liquid protecting tube 9, go into liquid sealing post 10, connect protecting tube 11, fluid sealing column 12, fluid protecting tube 13, sealing epoxy resin 14, pedestal 15 and several microdialysis probes 1.Wherein, microdialysis probe 1 comprises: hollow fiber polymer membrane 2, scaffold tube 4, go into liquid capillary tube 5 and fluid capillary tube 6, hollow fiber polymer membrane 2 is fixedly connected through epoxy resin 3 with scaffold tube 4; The inner chamber of hollow fiber polymer membrane 2 and scaffold tube 4 communicates, shown in pedestal 15 have some through holes, microdialysis probe 1 is separately fixed in the through hole of pedestal 15; Among this embodiment; The array microdialysis probe is that tandem connects, that is: the liquid capillary tube 5 of going into of first microdialysis probe 1 passes into liquid protecting tube 9, and the fluid capillary tube 6 of first microdialysis probe 1 is through after connecting protecting tube 11; Communicate with the liquid capillary tube 5 of going into of second microdialysis probe 1; The fluid capillary tube 6 of second microdialysis probe 1 communicates with the liquid capillary tube 5 of going into of the 3rd microdialysis probe 1 through after connecting protecting tube 11, and the like; The fluid capillary tube 6 of last microdialysis probe 1 passes fluid protecting tube 13; Go into liquid protecting tube 9 by going into 10 sealings of liquid sealing post, fluid sealing column 12 is by 13 sealings of fluid protecting tube, and the junction that go into liquid protecting tube 9, microdialysis probe 1, connects between protecting tube 11 and the fluid protecting tube 13 is connected with epoxy resin 8 by connecting tube.Going into liquid protecting tube 9, connection protecting tube 11 and fluid protecting tube 13 is sealed and is fixed on the pedestal 15 by sealing epoxy resin 14.
Among this embodiment, all microdialysis probes 1 are isometric, series connection each other, and the film that has increased effective dialysis is long, has improved the dialysis response rate.But microdialysis probe 1 can be not isometric yet, thereby the cerebral tissue that can adapt to simultaneously different depth carries out microdialysis simultaneously.
Among Fig. 1,4 grades microdialysis probe 1 of looking is arranged, but in actual production process, can freely adjust the number of microdialysis probe 1 as required.
Embodiment 2
As shown in Figure 2; Be with the difference of embodiment 1; Microdialysis probe 1 is divided into several groups, and every group of microdialysis probe 1 is isometric, and as shown in Figure 1; Go into liquid protecting tube 9, a fluid protecting tube 13 and some protecting tubes 11 that connects and form being connected in series relations with one respectively, not the length difference of the microdialysis probe 1 between on the same group.
Among this embodiment, the length of the microdialysis probe 1 between on the same group is not different, and does not form independently series relationship between on the same group, therefore, can be respectively the cerebral tissue of different depth be carried out microdialysis simultaneously, improves dialytic efficiency.
Among Fig. 2, microdialysis probe 1 is divided into different two groups of length, every group of two isometric microdialysis probe 1 and one go into liquid protecting tube 9, fluid protecting tube 13, one connect protecting tube 11 and form independently series relationship.But in actual production process, can freely adjust the group number of microdialysis probe 1 as required, and the number of every group microdialysis probe 1.
Embodiment 3
As shown in Figure 3, be that with the difference of embodiment 1 do not connect protecting tube 11, the liquid capillary tube 5 of going into of each microdialysis probe 1 passes the same liquid protecting tube 9 of going into respectively, fluid capillary tube 6 passes same fluid protecting tube 13, forms the relation of being connected in parallel.
Among this embodiment, comprise that one is gone into liquid protecting tube 9, fluid protecting tube 13 and some microdialysis probes 1, each some microdialysis probe 1 forms the annexation of parallel connection, can dialyse to multiple-point cerebral tissue simultaneously.
Among Fig. 3,4 microdialysis probes 1 are arranged, but in actual production process, can freely adjust the number of microdialysis probe 1 as required; The length of each microdialysis probe 1 is different, adapts to the vicissitudinous cerebral tissue microdialysis of the degree of depth, and in actual production process, the length of each microdialysis probe 1 also can be identical, carries out the cerebral tissue microdialysis of the same degree of depth.
The foregoing description is used for the present invention that explains, rather than limits the invention, and in the protection domain of spirit of the present invention and claim, any modification and change to the present invention makes all fall into protection scope of the present invention.
Claims (4)
1. array microdialysis probe; It is characterized in that it comprises: go into liquid protecting tube (9), go into liquid sealing post (10), fluid sealing column (12), fluid protecting tube (13), sealing epoxy resin (14), pedestal (15) and several microdialysis probes (1) etc.; Wherein, Microdialysis probe (1) comprising: hollow fiber polymer membrane (2), scaffold tube (4), go into liquid capillary tube (5) and fluid capillary tube (6) etc.; Hollow fiber polymer membrane (2) is fixedly connected through epoxy resin (3) with scaffold tube (4), and the inner chamber of hollow fiber polymer membrane (2) and scaffold tube (4) communicates; Pedestal (15) has some through holes; A microdialysis probe of each through hole internal fixation (1) of pedestal (15); Go into liquid protecting tube (9) by going into liquid sealing post (10) sealing; Fluid sealing column (12) forms the annexation of series, parallel or grouping by fluid protecting tube (13) sealing between each microdialysis probe (1).
2. according to the said array microdialysis probe of claim 1; It is characterized in that; Said placed in-line annexation is specially: the array microdialysis probe also comprises some connection protecting tubes (11) that are used to connect microdialysis probe (1); Go into liquid protecting tube (9) and fluid protecting tube (13) is one; The liquid capillary tube (5) of going into of first microdialysis probe (1) passes into liquid protecting tube (9); The fluid capillary tube (6) of first microdialysis probe (1) communicates with the liquid capillary tube (5) of going into of second microdialysis probe (1) after connecting protecting tube (11) through first, after the fluid capillary tube (6) of second microdialysis probe (1) connects protecting tube (11) through second; Communicate with the liquid capillary tube (5) of going into of the 3rd microdialysis probe (1); And the like, the fluid capillary tube (6) of last microdialysis probe (1) passes fluid protecting tube (13), and the junction of going between liquid protecting tube (9), microdialysis probe (1), connection protecting tube (11) and the fluid protecting tube (13) is connected with epoxy resin (8) by connecting tube; Going into liquid protecting tube (9), connection protecting tube (11) and fluid protecting tube (13) seals and is fixed on the pedestal (15) by sealing epoxy resin (14).
3. according to the said array microdialysis probe of claim 1; It is characterized in that; The annexation of said parallel connection is specially: go into liquid protecting tube (9) and fluid protecting tube (13) is one; The liquid capillary tube (5) of going into of each microdialysis probe (1) passes respectively into liquid protecting tube (9), and the fluid capillary tube (6) of each microdialysis probe (1) passes same fluid protecting tube (13); The junction of going between liquid protecting tube (9), microdialysis probe (1) and the fluid protecting tube (13) is connected with epoxy resin (8) by connecting tube; Go into liquid protecting tube (9) and fluid protecting tube (13) seals and is fixed on the pedestal (15) by sealing epoxy resin (14).
4. according to the said array microdialysis probe of claim 1; It is characterized in that; The annexation of said grouping is specially: the number of going into liquid protecting tube (9) and fluid protecting tube (13) is N, and microdialysis probe (1) is divided into the N group, and each length of organizing microdialysis probe (1) is different; The length of microdialysis probe (1) is identical on the same group; The number of every group of microdialysis probe (1) is 1 or greater than 1, and when the number of every group of microdialysis probe (1) is greater than 1 the time, each microdialysis probe (1) forms the annexation of serial or parallel connection.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110444617 CN102525569B (en) | 2011-12-28 | 2011-12-28 | Array type microdialysis probe |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110444617 CN102525569B (en) | 2011-12-28 | 2011-12-28 | Array type microdialysis probe |
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| CN102525569A true CN102525569A (en) | 2012-07-04 |
| CN102525569B CN102525569B (en) | 2013-12-25 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102809502A (en) * | 2012-08-21 | 2012-12-05 | 天津大学 | Microdialysis device for improving recovery rate of non-polar medicine |
| CN103308349A (en) * | 2013-06-09 | 2013-09-18 | 覃思 | Micro-dialysis sensor |
Citations (7)
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| US5882317A (en) * | 1996-09-20 | 1999-03-16 | Nec Corporation | Method and apparatus of sampling suction effusion fluid |
| CN1358994A (en) * | 2000-12-18 | 2002-07-17 | 中国科学院化学研究所 | Sensor type microdialysis biological living sampling device |
| CN1891143A (en) * | 2005-07-07 | 2007-01-10 | 中国科学院生物物理研究所 | High-density electrode array and its making method |
| CN201286706Y (en) * | 2008-10-31 | 2009-08-12 | 中国人民解放军第二军医大学 | Intravascular sampling micro-dialysis probe |
| US20090204195A1 (en) * | 2005-12-12 | 2009-08-13 | Jacobsen Stephen C | Multi-Element Probe Array |
| CN201822867U (en) * | 2009-11-23 | 2011-05-11 | 浙江大学 | Jugular vein micro dialysis probe and probe guide device for biological sampling |
| CN202408963U (en) * | 2011-12-28 | 2012-09-05 | 浙江省肿瘤医院 | Array type micro-dialysis probe |
-
2011
- 2011-12-28 CN CN 201110444617 patent/CN102525569B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5882317A (en) * | 1996-09-20 | 1999-03-16 | Nec Corporation | Method and apparatus of sampling suction effusion fluid |
| CN1358994A (en) * | 2000-12-18 | 2002-07-17 | 中国科学院化学研究所 | Sensor type microdialysis biological living sampling device |
| CN1891143A (en) * | 2005-07-07 | 2007-01-10 | 中国科学院生物物理研究所 | High-density electrode array and its making method |
| US20090204195A1 (en) * | 2005-12-12 | 2009-08-13 | Jacobsen Stephen C | Multi-Element Probe Array |
| CN201286706Y (en) * | 2008-10-31 | 2009-08-12 | 中国人民解放军第二军医大学 | Intravascular sampling micro-dialysis probe |
| CN201822867U (en) * | 2009-11-23 | 2011-05-11 | 浙江大学 | Jugular vein micro dialysis probe and probe guide device for biological sampling |
| CN202408963U (en) * | 2011-12-28 | 2012-09-05 | 浙江省肿瘤医院 | Array type micro-dialysis probe |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102809502A (en) * | 2012-08-21 | 2012-12-05 | 天津大学 | Microdialysis device for improving recovery rate of non-polar medicine |
| CN103308349A (en) * | 2013-06-09 | 2013-09-18 | 覃思 | Micro-dialysis sensor |
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| CN102525569B (en) | 2013-12-25 |
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