CN102599991A - Application of beautifying cornea lens - Google Patents
Application of beautifying cornea lens Download PDFInfo
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- CN102599991A CN102599991A CN2012100751533A CN201210075153A CN102599991A CN 102599991 A CN102599991 A CN 102599991A CN 2012100751533 A CN2012100751533 A CN 2012100751533A CN 201210075153 A CN201210075153 A CN 201210075153A CN 102599991 A CN102599991 A CN 102599991A
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- genipin
- cornea
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- lens
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Abstract
The invention discloses application of a beautifying cornea lens, and aims at applying the beautifying cornea lens to repairing the damage of cornea tissues, improving the appearance of an ocular surface, curing visual symptoms such as photophoby, glaring, diplopia and the like resulting from iris abnormity, or being used as a covering sheet or a medicine carrier for protecting the cornea in an ophthalmic operation, wherein the beautifying cornea lens is prepared in a way that: a biological material rich in amino is added into a solution containing genipin or genipin derivatives, the genipin is taken as a cross-linking agent to conduct the contact reaction, and then the mixture is rinsed, frozen and dried to prepare the beautifying cornea lens. The beautifying cornea lens provided by the invention can be used for repairing the damage of the cornea tissues, improving the appearance of the ocular surface, curing the visual symptoms such as photophoby, glaring, diplopia and the like resulting from iris abnormity, or being used as the covering sheet or the medicine carrier for protecting the cornea in the ophthalmic operation, therefore, a new approach is directly explored for clinical application.
Description
Technical field:
The invention belongs to field of medicaments, relate to the application of cosmetic corneal lens specifically.
Background technology:
The visual function that a variety of causes causes is badly damaged and blinding, merges the patient of cornea illness, damaged, cicatrix or leucocoria, and getable effective treatment at present is very limited.Blind person to merging corneal scarring has the scholar to attempt adopting corneal contact lens and corneal tattooing to improve its outward appearance.But; The tolerance compliance that contact lens is not good enough; And no matter the stain that the dyeing art is chosen is metallic or nonmetal character; Equal in various degree existing problems: painted disappear take off, the poor controllability of dyeing scope and shade, inflammatory reaction and because of stain biocompatibility difference or potential toxicity influence its safety, use at clinical expansion thereby limit it.And for merging cornea illness or damaged blind person, treatment is merely and improves outward appearance, considers that the cornea donor source is deficient, and this type of patient is getable rationally effective and not over-drastic treatment is undecided always.
In addition; Aniridia disease; The coloboma of iris that a variety of causes causes; And iris such as iris open postoperative diplopia is when causing visual qualities such as photophobia, dazzle, diplopia influenced unusually, and the selection that can supply at present is except that above-mentioned corneal contact lens and corneal tattooing, in addition with iris diaphragm intraocular lens's implantation.The problem that the above two exist is set forth.Potential risk and problem in that crystal eye implantation belt iris diaphragm intraocular lens is arranged are also more, such as: quicken cataractous incidence and development, intraocular pressure raises and then causes glaucoma, and the anterior chamber shoals and endothelial cell is lost etc.
The timbering material of organizational project, development at present is tending towards two types, and a kind of is synthetic material, and another kind is a natural material.The available physical space that often is merely of synthetic material; Even the modification in later stage can be added part chemistry and bio signal; Also be difficult to avoid its single limited effect, and required comprehensive regulation and control biology of the basic vital movement of cell require to greatly differ from each other in the organism.But natural material can be cell the microenvironment of depending on for existence is provided, and its preparation is obtained, and can obtain the maximum organic careful bionical integration of physical space, chemistry and bio signal simultaneously, is to achieve many things at one stroke.
Summary of the invention:
The purpose of this invention is to provide a kind of cosmetic corneal lens preparation be used for repairing cornea tissue damaged, improve vision ill symptomses such as the eye off-balancesheet is seen, the treatment iris causes unusually photophobia, dazzle, diplopia, as the opaque disk of ophthalmologic operation protection cornea or the application of pharmaceutical carrier.
Cosmetic corneal lens of the present invention preparation be used for repairing cornea tissue damaged, improve vision ill symptomses such as the eye off-balancesheet is seen, the treatment iris causes unusually photophobia, dazzle, diplopia, as the opaque disk of ophthalmologic operation protection cornea and the application of pharmaceutical carrier; Described cosmetic corneal lens is to join in the solution that contains genipin or genipin derivant being rich in amino biomaterial; Carry out haptoreaction with genipin as cross-linking agent, then rinsing and lyophilization and prepare.
The described biomaterial that is rich in amino is preferably the extracellular matrix of natural tissues, or external synthetic equivalent extracellular matrix.
The mass and size concentration of described genipin or genipin derivant is 0.001～100%.
The solution of described genipin or genipin derivant, its solvent are deionized water, various acid solution, various aqueous slkali, and in the organic solvent any one or more, and the pH value scope is 1～14, and temperature range is 0～56 ℃.
Preferably, when haptoreaction, vibrate or ultrasonic Treatment.
Described lyophilization precooling temperature scope is :-200～0 ℃, drying be aided with drying medium through normal pressure and/or reduced pressure with and stream, adverse current, and one or more realizations in the cross-flow mode.
Described cosmetic corneal lens color wavelength ranges is 380～740nm, and frequency range is 405～790THz, determines according to concrete patient.
Described cosmetic corneal lens thickness is 0.001～10mm, selects according to the patient of reality.
Described cosmetic corneal lens is 1～100mm
2Annular, ellipse, triangle, the tetragon of size, and in irregularly shaped any such as crescent are selected different sizes and shape according to concrete sufferer situation.
Cosmetic corneal lens of the present invention is the tissue engineering bracket material of being processed by extracellular matrix; Its preparation method belongs to the technology of comparative maturity in the prior art; This cosmetic corneal lens comes from extracellular matrix, has good substrate microenvironment, can safe long-term implantation ophthalmic.Amino in genipin or genipin derivant and the extracellular matrix reacts the colour stable that forms, and can strengthen the mechanical strength of extracellular matrix.Genipin or genipin derivant have different response speeds under different concentration, solvent, pH value, temperature conditions; Therefore can come the painted requirement of control extracellular matrix flexibly through regulating these parameters; This belongs to the conventional knowledge of this area; Thereby to adapt to the iris color requirement of different ethnic groups, perhaps special color demand.Painted extracellular matrix can be used for the dark place function of cosmetic eye table operation and alternative iris.
The present invention finds through experiment; Cosmetic corneal lens of the present invention can be used for repairing cornea tissue damaged, improve vision ill symptoms such as the eye off-balancesheet is seen, the treatment iris causes unusually photophobia, dazzle, diplopia or, therefore directly opened up new way for clinical practice as the opaque disk of ophthalmologic operation protection cornea.
The specific embodiment:
Following examples are to further specify of the present invention, rather than limitation of the present invention.
Embodiment 1:
The purpose of present embodiment be with the cosmetic corneal lens be used for anterior corneal surface eyeglass art repair cornea tissue damaged, improve the eye off-balancesheet and see.
The method for preparing of cosmetic corneal lens: will take off cell porcine cornea substrate and join in 0.5% (mass and size concentration) genipin aqueous solution (pH value is 7.0); Oscillation treatment is 8 hours in 20 ℃ of water-baths, and rinsing and lyophilization prepare sepia cosmetic corneal lens.This eyeglass is a sepia, and thickness is 0.1～0.4mm, circular (concrete shape and specification look case and decide).
By the requirement of anterior corneal surface eyeglass art, make embedding groove at anterior corneal surface, in the poor circumferential edges insertion groove with sepia cosmetic corneal lens, fix pressure dressing art eye with suture or biogum.Press anterior corneal surface eyeglass postoperative conventional treatment.Damaged the obtaining of the cornea tissue of postoperative patient repaired, and corneal scarring improves in appearance.
Embodiment 2:
The purpose of present embodiment is the cosmetic corneal lens to be used for implantation between corneal stroma treat vision ill symptomses such as photophobia that iris causes unusually, dazzle, diplopia.
The method for preparing of cosmetic corneal lens: collagen cornea shield is joined in 2% (mass and size concentration) genipin alcoholic solution (pH value is 9.0); Oscillation treatment is 1 hour in 56 ℃ of water-baths; Rinsing and lyophilization prepare black cosmetic corneal lens; This cosmetic corneal lens color is a black, and thickness is 0.1mm, annular (concrete shape and specification look case and decide).
Be used to treat irises such as coloboma of iris that aniridia disease, a variety of causes cause and iris open postoperative diplopia and cause photophobia, dazzle, diplopia etc. unusually.Make corneal stroma interlayer capsule bag, in black cosmetic corneal lens implant angle membrane matrix interlayer, after fixing, pressure dressing art eye.Press and implant the postoperative conventional treatment between corneal stroma.The iris dark place function of postoperative patient will be substituted by the black cosmetic corneal lens of implanting, and the vision ill symptoms improves.
Embodiment 3:
The purpose of present embodiment is with the eye table opaque disk of cosmetic corneal lens as protection cornea in the ophthalmologic operation.
The method for preparing of cosmetic corneal lens: cell rabbit corneal substrate is taken off in lyophilizing join in 0.01% (mass and size concentration) genipin aqueous solution (pH value is 4.0); Oscillation treatment half an hour in 37 ℃ of water-baths; Rinsing and lyophilization prepare white cosmetic corneal lens; This cosmetic corneal lens color is a white, and thickness is 0.5mm, the circle of 5～10mm diameter.
As eye socket operation, eyelid operation, eye muscle operation, conjunctival operation, and the eye table opaque disk of protecting cornea in the operation for glaucoma.White cosmetic corneal lens is immersed normal saline after 5 minutes, place anterior corneal surface.As biomaterial, can keep the eye table moistening and protected, simultaneously also because of the light tight operation source property photostimulation damage of protecting macula lutea.
Claims (9)
- The cosmetic corneal lens preparation be used for repairing cornea tissue damaged, improve vision ill symptomses such as the eye off-balancesheet is seen, the treatment iris causes unusually photophobia, dazzle, diplopia, as the opaque disk of ophthalmologic operation protection cornea or the application of pharmaceutical carrier; Described cosmetic corneal lens is to join in the solution that contains genipin or genipin derivant being rich in amino biomaterial; Carry out haptoreaction with genipin as cross-linking agent, then rinsing and lyophilization and prepare.
- 2. application according to claim 1 is characterized in that, the described biomaterial that is rich in amino is the extracellular matrix of natural tissues, or external synthetic equivalent extracellular matrix.
- 3. application according to claim 1 is characterized in that, the mass and size concentration of described genipin or genipin derivant is 0.001～100%.
- 4. according to claim 1 or 3 described application, it is characterized in that the solution of described genipin or genipin derivant; Its solvent is deionized water, acid solution, aqueous slkali; And in the organic solvent any one or more, the pH value scope is 1～14, temperature range is 0～56 ℃.
- 5. application according to claim 1 is characterized in that, when haptoreaction, vibrates or ultrasonic Treatment.
- 6. application according to claim 1; It is characterized in that; Described lyophilization precooling temperature scope is :-200～0 ℃, drying be aided with drying medium through normal pressure and/or reduced pressure with and stream, adverse current, and one or more realizations in the cross-flow mode.
- 7. application according to claim 1 is characterized in that, described cosmetic corneal lens color wavelength ranges is 380～740nm, and frequency range is 405～790THz.
- 8. application according to claim 1 is characterized in that, described cosmetic corneal lens thickness is 0.001～10mm.
- 9. application according to claim 1 is characterized in that, described cosmetic corneal lens is 1～100mm 2During the annular, ellipse, triangle, tetragon, crescent of size or other are irregularly shaped any.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100751533A CN102599991A (en) | 2012-03-20 | 2012-03-20 | Application of beautifying cornea lens |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100751533A CN102599991A (en) | 2012-03-20 | 2012-03-20 | Application of beautifying cornea lens |
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| CN102599991A true CN102599991A (en) | 2012-07-25 |
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| CN2012100751533A Pending CN102599991A (en) | 2012-03-20 | 2012-03-20 | Application of beautifying cornea lens |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109069264A (en) * | 2016-12-16 | 2018-12-21 | 厦门大开生物科技有限公司 | A kind of drying means and the dry cornea of de- cell pig plate layer of de- cell cornea |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109069264A (en) * | 2016-12-16 | 2018-12-21 | 厦门大开生物科技有限公司 | A kind of drying means and the dry cornea of de- cell pig plate layer of de- cell cornea |
| CN109069264B (en) * | 2016-12-16 | 2020-06-30 | 厦门大开生物科技有限公司 | Drying method of acellular cornea and acellular pig lamina layer dried cornea |
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Application publication date: 20120725 |