CN102680610A - Gas chromatographic column separated through electric filed control and applications thereof - Google Patents
Gas chromatographic column separated through electric filed control and applications thereof Download PDFInfo
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- CN102680610A CN102680610A CN2011100662520A CN201110066252A CN102680610A CN 102680610 A CN102680610 A CN 102680610A CN 2011100662520 A CN2011100662520 A CN 2011100662520A CN 201110066252 A CN201110066252 A CN 201110066252A CN 102680610 A CN102680610 A CN 102680610A
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- chromatographic column
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- electric field
- gas chromatographic
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Abstract
The invention relates to a gas chromatographic column, which is a chromatographic technology for controlling the retention time of compounds in a chromatograph by using an electric filed. Through enabling an internal electrode to pass through the gas chromatographic column, sleeving an external electrode on the gas chromatographic column, and exerting pressure on the electrodes, different electric field strengths can be obtained; and the electric field intensity affects the retention time of sample molecules in the chromatographic column, thereby realizing the separation of different samples.
Description
Technical field
The present invention relates to gas chromatographic column, is a kind of chromatographic technique that utilizes the retention time of electric field controls compound in chromatogram.
Background technology
The separating power of chromatographic column is very limited, often is difficult to realize separating for the close compound of structure.The method that changes chromatogram separating capacity at present mainly is to change the chromatographic column or the chromatographic column inner stuffing of different model, and these method costs are higher, and it is time-consuming to require great effort.
Polar molecule can increase polarity because of the polarization of electric field in electric field, non-polar molecule also can produce moment polarity.Therefore, can change molecular polarity through the size of electric field intensity.This patent changes the polarity of molecule in chromatogram through in chromatographic column, adding lead and applying certain electric field, thereby the retention time of molecule in chromatogram also can be changed.Because electric field level can pass through external adjustment easily, so the present invention can change the retention time of sample molecule in chromatographic column through controlling voltage, thereby reaches the separation of opposed polarity compound.
Summary of the invention
The object of the present invention is to provide a kind of chromatographic technique that utilizes the retention time of electric field controls compound in chromatogram.
For realizing above-mentioned purpose, the structure that the present invention adopts is:
A kind of gas chromatographic column of electric field controls comprises in the chromatographic column, interior electrode and external electrode,
Interior electrode is a line electrode, interior electrode along chromatographic column axially in chromatographic column, dispatch from foreign news agency is the round barrel shape electrode very, it is nested with in chromatographic column outside.
The present invention can change the retention time of compound in chromatographic column easily, thereby realizes the separation of opposed polarity molecule.
Different according to voltage applying mode, the different techniques scheme is arranged.
Apply DC voltage on the electrode in said, magnitude of voltage is-10kV to 10kV;
Or applying alternating voltage on the electrode in said, the Voltage Peak peak value is 0-10kV;
Or applying the DC voltage of even increase in said on the electrode, voltage is advanced the speed and is 0.01V/ second to 1000V/ second.
Apply DC voltage on the said external electrode, magnitude of voltage is-10kV to 10kV;
Or, applying alternating voltage on the said external electrode, the Voltage Peak peak value is 0-10kV;
Or, applying the DC voltage of even increase on the said external electrode, voltage is advanced the speed and is 0.01V/ second to 1000V/ second.
The present invention is simple and easy to usefulness, can change the retention time of some sample molecule in chromatographic column through electric field, thereby reach the effect of sample separation, has avoided need changing the cost that chromatographic column is brought in the past.
Description of drawings
Fig. 1 is a structural representation of the present invention.
Embodiment
It is as shown in Figure 1,
Electrode 2 in gas chromatographic column, being penetrated with (line electrode, it is a metal wire) is with external electrode 3 (it is the electrode of metal material cylinder-like structures preparation, two end openings) in chromatographic column 1 outside, interior electrode and external electrode constitute electrode.On interior electrode and external electrode, apply voltage; In chromatographic column, obtain different electric field intensity; The size of electric field intensity can influence the retention time of sample molecule in chromatographic column; Promptly according to the difference of voltage, the retention time of sample molecule in chromatographic column is also inequality, thereby realizes the separation of different samples.
Embodiment 1
On interior electrode, apply positive 500V voltage, external electrode ground connection at this moment, can form powerful electric field in chromatographic column.
The capillary column of chromatographic column adopting 10 m * 0.32 mm * 1.0 μ m, column temperature are 50
oC (0.5 min) is to 280
oC, heating rate are 15
oC/min uses He to be carrier gas, and flow velocity is 40 ml/s, and detecting device uses fid detector.Stationary phase adopts 35% diphenyl, 65% dimethyl polysiloxane immobile liquid, and benzene is separated with monochloro-benzene and analyzes.
Compare during with making alive not, the polarity of monochloro-benzene increases, and the assigned degree in polar stationary phase changes, so the retention time of monochloro-benzene in chromatographic column also increases.The molecule retention time that polarity is strong more changes big more.
Chromatographic peak retention time under more a plurality of voltages is made the relation of benzene and monochloro-benzene retention time and voltage respectively, selects suitable voltage to make benzene and monochloro-benzene reach optimal separation then.
Embodiment 2
On interior electrode, apply the positive voltage of even increase, voltage is advanced the speed and is 100V/ second, uses polar stationary phase in the chromatographic column.
The capillary column of chromatographic column adopting 10 m * 0.32 mm * 2.0 μ m, column temperature are 50
oC (0.5 min) is to 280
oC, heating rate are 15
oC/min uses He to be carrier gas, and flow velocity is 20 ml/s, and detecting device uses fid detector.Stationary phase adopts 15% diphenyl, 85% dimethyl polysiloxane immobile liquid, and benzene is separated with monochloro-benzene and analyzes.
Because the retention time of polar molecule monochloro-benzene in high electric field increases, and the change of non-polar molecule benzene is very little, therefore can the low pole molecule be separated with non-polar molecule.Obtain the rate of change of electric field intensity and the relation of polar molecule retention time through experiment, be depicted as working curve, to obtain the separation of optimum target sample.
Claims (2)
1. the gas chromatographic column that separates of an electric field controls is characterized in that: comprise in the chromatographic column (1), interior electrode (2) and external electrode (3),
Interior electrode (2) is a line electrode, interior electrode (2) along chromatographic column (1) axially in chromatographic column (1),
External electrode (3) is the round barrel shape electrode, and it is nested with in chromatographic column (1) outside.
2. the application of the gas chromatographic column of the said electric field controls separation of claim 1 is characterized in that:
Apply DC voltage on the electrode (2) in said, magnitude of voltage is-10kV to 10kV;
Or, applying alternating voltage on the said interior electrode (2), the Voltage Peak peak value is 0-10kV;
Or, applying the DC voltage of even increase in said on the electrode (2), voltage is advanced the speed and is 0.01V/ second to 1000V/ second;
Said external electrode applies DC voltage on (3), and magnitude of voltage is-10kV to 10kV;
Or said external electrode applies alternating voltage on (3), and the Voltage Peak peak value is 0-10kV;
Or, applying the DC voltage of even increase on the said external electrode (3), voltage is advanced the speed and is 0.01V/ second to 1000V/ second.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100662520A CN102680610A (en) | 2011-03-18 | 2011-03-18 | Gas chromatographic column separated through electric filed control and applications thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100662520A CN102680610A (en) | 2011-03-18 | 2011-03-18 | Gas chromatographic column separated through electric filed control and applications thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102680610A true CN102680610A (en) | 2012-09-19 |
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| CN2011100662520A Pending CN102680610A (en) | 2011-03-18 | 2011-03-18 | Gas chromatographic column separated through electric filed control and applications thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103691318A (en) * | 2014-01-07 | 2014-04-02 | 延边大学 | Micro-scale substance separating method and capillary column transverse eletrochromatography separating device |
| CN111265914A (en) * | 2020-03-13 | 2020-06-12 | 刘楷 | Electric field chromatographic column |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1379242A (en) * | 2001-04-02 | 2002-11-13 | 王少坤 | Chromatographic electric field separating method and device |
| CN2911691Y (en) * | 2006-06-21 | 2007-06-13 | 戴朝政 | Detention eliminating chromatograhic column |
| CN102012411A (en) * | 2010-10-22 | 2011-04-13 | 戴朝政 | Stagnation-eliminating chromatographic column, stagnation-eliminating chromatograph and analysis method thereof |
-
2011
- 2011-03-18 CN CN2011100662520A patent/CN102680610A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1379242A (en) * | 2001-04-02 | 2002-11-13 | 王少坤 | Chromatographic electric field separating method and device |
| CN2911691Y (en) * | 2006-06-21 | 2007-06-13 | 戴朝政 | Detention eliminating chromatograhic column |
| CN102012411A (en) * | 2010-10-22 | 2011-04-13 | 戴朝政 | Stagnation-eliminating chromatographic column, stagnation-eliminating chromatograph and analysis method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103691318A (en) * | 2014-01-07 | 2014-04-02 | 延边大学 | Micro-scale substance separating method and capillary column transverse eletrochromatography separating device |
| CN111265914A (en) * | 2020-03-13 | 2020-06-12 | 刘楷 | Electric field chromatographic column |
| CN111265914B (en) * | 2020-03-13 | 2022-05-20 | 刘楷 | Electric field chromatographic column |
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Application publication date: 20120919 |