CN102691083A - Electrochemical method for improving blood compatibility of surface of metal material - Google Patents
Electrochemical method for improving blood compatibility of surface of metal material Download PDFInfo
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- CN102691083A CN102691083A CN2012101691991A CN201210169199A CN102691083A CN 102691083 A CN102691083 A CN 102691083A CN 2012101691991 A CN2012101691991 A CN 2012101691991A CN 201210169199 A CN201210169199 A CN 201210169199A CN 102691083 A CN102691083 A CN 102691083A
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Abstract
The invention relates to an electrochemical method for improving the blood compatibility of the surface of a metal material. The electromechanical method comprises the following steps: connecting a metal material to be modified onto a positive electrode of a direct current power supply; immersing the metal material in an aqueous solution containing phenol; and modifying by electrifying; soaking the modified metal material in aqueous solution containing heparin to obtain the metal material with blood compatibility. Compared with a traditional standing coating modification process which has the defects of long modification time, poor attaching power of a coating, non-uniformity in surface and the like, the electromechanical method disclosed by the invention has the advantages that the modification time is greatly shortened and the obtained coating is strong in attaching power and more uniform in surface.
Description
Technical field
The present invention relates to a kind of electrochemical method that improves the blood compatibility of metal material surface.
Background technology
Electrochemical method is to be the initiation power and the motivating force of polyreaction with the electropotential, makes monomer in electrode surface direct polymerization film forming.Electrochemical method is fairly simple, and it has some unique advantages: (1) polymerization is carried out with mixing simultaneously; (2) can be on electrode a step generate the conducting film of self-supporting; (3) can distinguish the redox state of controlling diaphragm easily through changing polymerization potential; (4) thickness of film can be controlled through electric current and voltage; (5) product need not separating step; (6) on electrode, generating the polymkeric substance of process and the generation of polymkeric substance can be easily through electrochemistry or spectral method research, without catalyzer etc., so has obtained broad research.Electrochemical method comprises cyclic voltammetry, potentiostatic method, galvanostatic method etc.Cathodic reduction method in the electrochemical method is because the conductivity of the conducting polymer film that obtains at electrode surface is poor, and film thickness is difficult to surpass 100nm; So study fewerly.Therefore electrochemical method generally is meant anonizing.
Heparin is a kind of acidic mucopolysaccharide, mainly is to be produced by mastocyte and basophilic granulocyte.Very strong blood coagulation resisting function is all arranged in vivo and in vitro, so clinical widely-used as antithrombotics it.Heparin is widely used in preventing and treating the early treatment and the external anti-freezing of thrombotic disease, disseminated intravascular coagulation clinically.Clinical application and research show: heparin also has multiple biological activity and clinical application except that having anticoagulation.Along with pharmacology and clinical medical progress, the application of heparin constantly enlarges.
Summary of the invention
To the defective that prior art exists, the present invention aims to provide a kind of electrochemical method that improves the blood compatibility of metal material surface, is to material surface, and particularly the metallic surface applies phenols and the grafting heparin carries out twice-modified method fast.Promptly formerly in the electrolyzer material is immersed in the aqueous solution of phenol, electric polymerization reaction takes place in the metallic surface.Its characteristics mainly are to shorten the coating time greatly, and the coating that obtains is more even, fine and close, and sticking power is stronger.The material that will apply again is immersed in the heparin solution, obtains the metallic substance of excellent blood compatibility.
For achieving the above object, the present invention adopts following technical scheme:
A kind of electrochemical method that improves the metal material surface blood compatibility contains the metallic substance immersion in the aqueous solution of phenol in electrolyzer, and at metal material surface generation electric polymerization reaction, the metallic substance that will apply again is immersed in the heparin solution; Concrete technological process is:
1) adopts 1%Na
2SO
4The aqueous solution takes by weighing 0.2 ~ 0.6g phenol as solvent under the room temperature, be dissolved in the solution, and the concentration of solution is 2 ~ 6mg/ml;
2) metallic substance to be coated is immersed in the phenol aqueous solution, anode generation electropolymerization in electrolyzer, the required reaction times is 10 ~ 30min;
3) coated metallic substance is immersed in the heparin solution, the concentration of heparin solution is 10mg/mL, and the reaction times is 24h, takes out the unnecessary heparin of washing.
Than prior art, the inventive method advantage and effect can be concluded narration as follows:
1) electrochemical method rapid and convenient is compared general moving then several hours chemical reaction, just can accomplish in tens minutes.
2) redox reaction directly takes place in electrochemical method on electrode, need not use extra chemical reagent, the cheap environmental protection of reaction cost.
3) heparin can directly be coupling on the coating, need not coupling agent, clean environment firendly.Resulting coating adhesion is strong, and the surface is more even.
Description of drawings
Fig. 1 is a process flow diagram of the present invention;
Fig. 2 is a reforming apparatus synoptic diagram of the present invention.
Embodiment
As shown in Figure 2, material of the present invention shows the device synoptic diagram of modification.Comprise and treat modification base material 1, contain the aqueous solution 2 and direct supply 3 of phenols.
As shown in Figure 1, a kind of electrochemical method that improves the metal material surface blood compatibility immerses metallic substance in the aqueous solution of phenol in electrolyzer, and at metal material surface generation electric polymerization reaction, the metallic substance that will apply again is immersed in the heparin solution; Concrete technological process is:
1) adopts 1%Na
2SO
4The aqueous solution takes by weighing 0.2 ~ 0.6g phenol as solvent under the room temperature, be dissolved in the solution, and the concentration of solution is 2 ~ 6mg/ml;
2) metallic substance to be coated is immersed in the phenol aqueous solution, anode generation electropolymerization in electrolyzer, the required reaction times is 10 ~ 30min;
3) coated metallic substance is immersed in the heparin solution, the concentration of heparin solution is 10mg/mL, and the reaction times is 24h, takes out the unnecessary heparin of washing.
1) adopts 100mL1%Na
2SO
4The aqueous solution is got the 0.6g pyrocatechol and is dissolved in wherein as solvent, is made into the solution of 6mg/mL;
2) stainless steel substrates is connected on the dc power anode, the Pt electrode is a negative pole, and voltage is 2V, is immersed in the phenol aqueous solution electrochemical coating 10 minutes;
3) again that modification is good stainless steel soaks sheet not in the 10mg/mL heparin solution, and the reaction times is 24h, takes out the unnecessary heparin of washing.
1) adopts 100mL1%Na
2SO
4The aqueous solution is got the 0.2g pyrocatechol and is dissolved in wherein as solvent, is made into the solution of 2mg/mL;
2) stainless steel substrates is connected on the dc power anode, the Pt electrode is a negative pole, and voltage is 2V, is immersed in the phenol aqueous solution electrochemical coating 30 minutes;
3) again that modification is good stainless steel substrates is immersed in the 10mg/mL heparin solution, and the reaction times is 24h, takes out the unnecessary heparin of washing.
1) adopts 100mL1%Na
2SO
4The aqueous solution is got the 0.2g pyrocatechol and is dissolved in wherein as solvent, is made into the solution of 2mg/mL;
2) the titanium sheet is connected on the dc power anode, the Pt electrode is a negative pole, and voltage is 2V, is immersed in the phenol aqueous solution electrochemical coating 30 minutes;
3) again that modification is good titanium sheet is immersed in the 10mg/mL heparin solution, and the reaction times is 24h, takes out the unnecessary heparin of washing.
Embodiment 4
1) adopts 100mL1%Na
2SO
4The aqueous solution is got the 0.4g pyrocatechol and is dissolved in wherein as solvent, is made into the solution of 4mg/mL;
2) the titanium sheet is connected on the dc power anode, the Pt electrode is a negative pole, and voltage is 2V, is immersed in the phenol aqueous solution electrochemical coating 20 minutes;
3) again that modification is good titanium sheet is immersed in the 10mg/mL heparin solution, and the reaction times is 24h, takes out the unnecessary heparin of washing.
Embodiment 5
1) adopts the 100mL1%Na2SO4 aqueous solution as solvent, get the 0.2g Dopamine HCL and be dissolved in wherein, be made into the solution of 2mg/mL;
2) the titanium sheet is connected on the dc power anode, the Pt electrode is a negative pole, and voltage is 2V, is immersed in the phenol aqueous solution electrochemical coating 30 minutes;
3) again that modification is good titanium sheet is immersed in the 10mg/mL heparin solution, and the reaction times is 24h, takes out the unnecessary heparin of washing.
Embodiment 6
1) adopts the 100mL1%Na2SO4 aqueous solution as solvent, get the 0.6g Dopamine HCL and be dissolved in wherein, be made into the solution of 6mg/mL;
2) stainless steel substrates is connected on the dc power anode, the Pt electrode is a negative pole, and voltage is 2V, is immersed in the phenol aqueous solution electrochemical coating 10 minutes;
3) again that modification is good stainless steel soaks sheet not in the 10mg/mL heparin solution, and the reaction times is 24h, takes out the unnecessary heparin of washing.
Claims (1)
1. electrochemical method that improves the metal material surface blood compatibility; It is characterized in that; In electrolyzer, the metallic substance immersion is contained in the aqueous solution of phenol, at metal material surface generation electric polymerization reaction, the metallic substance that will apply again is immersed in the heparin solution; Concrete technological process is:
1) adopts 1%Na
2SO
4The aqueous solution takes by weighing 0.2 ~ 0.6g phenol as solvent under the room temperature, be dissolved in the solution, and the concentration of solution is 2 ~ 6mg/ml;
2) metallic substance to be coated is immersed in the phenol aqueous solution, anode generation electropolymerization in electrolyzer, the required reaction times is 10 ~ 30min;
3) coated metallic substance is immersed in the heparin solution, the concentration of heparin solution is 10mg/mL, and the reaction times is 24h, takes out the unnecessary heparin of washing.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104060311A (en) * | 2014-06-16 | 2014-09-24 | 浙江大学 | Method for functionally modifying surface of conductive substrate |
| US11086177B2 (en) | 2015-01-26 | 2021-08-10 | Samsung Display Co., Ltd. | Display apparatus |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1543362A (en) * | 2002-05-09 | 2004-11-03 | ����Ī�����ɷ�����˾ | Medical products comprising a haemocompatible coating, production and use thereof |
| CN101724841A (en) * | 2008-10-10 | 2010-06-09 | 北京化工大学 | Method for preparing polymer/silver composite membrane by depositing dopamine |
| US20100330025A1 (en) * | 2002-07-19 | 2010-12-30 | Northwestern University | Surface Independent, Surface-Modifying, Multifunctional Coatings and Applications Thereof |
| CN102000658A (en) * | 2010-12-15 | 2011-04-06 | 西南交通大学 | Polydopamine-based biofunction modification method |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1543362A (en) * | 2002-05-09 | 2004-11-03 | ����Ī�����ɷ�����˾ | Medical products comprising a haemocompatible coating, production and use thereof |
| US20100330025A1 (en) * | 2002-07-19 | 2010-12-30 | Northwestern University | Surface Independent, Surface-Modifying, Multifunctional Coatings and Applications Thereof |
| CN101724841A (en) * | 2008-10-10 | 2010-06-09 | 北京化工大学 | Method for preparing polymer/silver composite membrane by depositing dopamine |
| CN102000658A (en) * | 2010-12-15 | 2011-04-06 | 西南交通大学 | Polydopamine-based biofunction modification method |
Non-Patent Citations (4)
| Title |
|---|
| FALK BERNSMANN ET AL.: ""Characterization of Dopamine-Melanin Growth on Silicon Oxide"", 《J. PHYS. CHEM. C》, vol. 113, no. 19, 14 April 2009 (2009-04-14), pages 8234 - 8242 * |
| HAESHIN LEE ET AL.: ""Mussel-Inspired Surface Chemistry for Multifunctional Coatings"", 《SCIENCE》, vol. 318, 19 October 2007 (2007-10-19), pages 8234 - 8242 * |
| INSEONG YOU ET.AL.: ""Enhancement of Blood Compatibility of Poly(urethane) Substrates by Mussel-Inspired Adhesive Heparin Coating"", 《BIOCONJUGATE CHEMISTRY》, vol. 22, 16 June 2011 (2011-06-16), pages 1264 - 1269 * |
| 徐又一: ""多巴胺的自聚-附着行为与膜表面功能化"", 《膜科学与技术》, vol. 31, no. 3, 30 June 2011 (2011-06-30), pages 32 - 38 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104060311A (en) * | 2014-06-16 | 2014-09-24 | 浙江大学 | Method for functionally modifying surface of conductive substrate |
| US11086177B2 (en) | 2015-01-26 | 2021-08-10 | Samsung Display Co., Ltd. | Display apparatus |
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