CN102718599A - Preparation of compound rice dedicated bio-fertilizer based on arbuscular mycorrhizal fungi and application thereof - Google Patents
Preparation of compound rice dedicated bio-fertilizer based on arbuscular mycorrhizal fungi and application thereof Download PDFInfo
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- CN102718599A CN102718599A CN2012102290442A CN201210229044A CN102718599A CN 102718599 A CN102718599 A CN 102718599A CN 2012102290442 A CN2012102290442 A CN 2012102290442A CN 201210229044 A CN201210229044 A CN 201210229044A CN 102718599 A CN102718599 A CN 102718599A
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Abstract
The invention discloses a preparation of compound rice dedicated bio-fertilizer based on arbuscular mycorrhizal fungi and an application thereof, and relates to the preparation and the application of bio-fertilizer. The compound rice dedicated bio-fertilizer aims to solve the problem that rice can not be infected by the arbuscular mycorrhizal fungi under the aquatic condition at present and is difficult to effectively form mutualistic symbiosis, and therefore the rice can not obtain mineral nutrition from an arbuscular mycorrhizal fungi-crop symbiotic system. The method comprises the following steps of: preparing a colonized reinforcer; pre-processing arbuscular mycorrhizal fungi fungicide; and evenly mixing the processed arbuscular mycorrhizal fungi fungicide, rice shoots growth promoter and the colonized reinforce. The compound rice dedicated bio-fertilizer can be prepared into a seedling bed, culturing the mycorrhiza seedling and managing a rice field. According to the compound rice dedicated bio-fertilizer disclosed by the invention, the arbuscular mycorrhizal fungi-crop symbiotic system with a high infection rate and stable performance can be built in the seedling stage of the rice, and the symbiotic system can successfully live through the transplanted field flooding environment. The biological bactericide activity loss problem brought by flooding intimidation can be effectively solved, mineral nutrition is continuously provided for the rice, the growth situation of the rice is improved, the fertilizer application amount in the field is reduced, and the rice production cost can be effectively lowered.
Description
Technical field
The present invention relates to the preparation and the application thereof of paddy rice special bio fertilizer.
Background technology
Nitrogen and phosphorus are the essential macroelement of paddy growth, and nitrogen phosphorus shortage can cause the crop dysplasia, finally causes declining to a great extent of rice yield and quality.Significant contribution has been made in the growth that is used for whole world paddy rice today of chemical fertilizer.Yet the rice yield growth that chemical fertilizer is depended on unduly is a cost to sacrifice ecotope.Paddy rice is very low to the utilization ratio of chemical fertilizer, and unabsorbed nutrition then causes environmental pollution through modes such as seepage, rainwash, denitrifications.In addition, the greenhouse gases of generation are used in nitrogen fertilizer production and farmland thereof, like CO
2, N
2O and NO
x, also more and more big to the influence of Global climate change.Simultaneously, the excessive exploitation of phosphorus ore has also caused the exhaustion of phosphate rock resource.Rice biological fertilizer has increases soil fertility, and promotes Soil Mineral Nutrition to discharge, and reduces disease and pest, improves functions such as rice quality and raising output.Yet the bio-feritlizer that is used for Rice Production at present can only be worked in the non-waterflooding phase of paddy growth by the water logging condition restriction; The fertilizer efficiency time length is short; Effect stability property is poor, and manufacture craft is loaded down with trivial details, and store method is complicated; Function singleness is difficult to effectively use in rice on honda production and big area is promoted.
Summary of the invention
The present invention seeks to be infected by bush mycorrhizal fungi at aquatic conditions in order to solve present paddy rice; Be difficult to effectively form symbiosis; Cause paddy rice can not obtain the problem of mineral nutrition, and preparation and the application thereof based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi is provided from arbuscular mycorrhizal fungus-do cogeneration system.
Preparation based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi; Carry out according to the following steps: the preparation of, growing fortifier surely: 1kg butch clover seed adds 1.5kg water and 0.001kg ammonium molybdate; Soak behind the 12h with distilled water flushing 3 ~ 5 times, control is dried then;
Two, the pre-treatment of bush mycorrhizal fungi microbial inoculum: butch clover root in the bush mycorrhizal fungi microbial inoculum is shredded to 1 ~ 2cm, the matrix in the bush mycorrhizal fungi microbial inoculum was smashed 10 order soil sieves, be the bush mycorrhizal fungi microbial inoculum of handling well behind the two mixing;
Three, with the bush mycorrhizal fungi microbial inoculum of handling well, rice seedling-growth soil regulator with grow fortifier surely by (1500 ~ 2500): (300 ~ 600): 1 volume ratio mixing, promptly accomplish preparation based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi.
The application of above-mentioned compound paddy rice special bio fertilizer based on bush mycorrhizal fungi, carry out according to the following steps:
One, field soil is tiled in the seedling dish, thickness is 1.5cm, adds the compound paddy rice special bio fertilizer 250g based on bush mycorrhizal fungi, processes the seedling bed;
Two, water spray is sprinkled into the 40g percentage of germination uniformly and reaches 95% ~ 98% rice paddy seed to drenched fully on the seedling bed, and rice paddy seed three faces bury, seedling bed earthing then, thickness is 0.5cm, by the rice shoot control measures of routine cultivated 30 days the mycorhiza seedling;
Three, be 36m at area
2The rice field in establish the wide guard rows of 1m; The border is done to cut off with the geomembrane of high 80cm and is handled; Wherein 50cm is underground hydrology blocking-up; 30cm is hydrology blocking-up on the ground, manages the rice field by the high yield liquid manure mode of routine, promptly accomplishes the application based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi.
The present invention is based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi, make paddy rice can set up the arbuscular mycorrhizal fungus of high infection rate, stable performance-do cogeneration system in the seedling phase.And this cogeneration system can be tided over the Honda water logging environment after the transplanting smoothly; Effectively solve water logging and coerce the bacteria agent loss of activity problem of bringing; Be continuously paddy rice mineral nutrition is provided; Improve the upgrowth situation of paddy rice, reduce the amount of application of this Tanaka chemical fertilizer, alleviate the negative impact that chemical fertilizer causes surface water and underground water.In addition, amount of application of the present invention is little, and the short of paddy rice come into force fruit obviously, can effectively reduce the cost of Rice Production.Working method of the present invention is simple simultaneously, is easy to preserve, and is fit to large-scale promotion application.
The present invention is based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi, the paddy rice whole growth cycle is all had significant promoter action.First, the use of the present invention rice seedlings, seedlings thrive, side stems wide, with Nie rate, rooting ability, reviving fast, high yield performance.The present invention simultaneously can reduce kind and the quantity of rice seedling bed weeds, for the growth of rice seedling provides good ecotope.In addition, the stage applies this fertilizer and can promote growing of Honda stage paddy rice in the rice seedling bed, is embodied in and can improves the paddy rice photosynthetic efficiency, improves the paddy rice dispensed materials, improves rice yield.The compound paddy rice special bio fertilizer that the present invention is based on bush mycorrhizal fungi all has the good short fruit of coming into force to south rice (Jiangsu Province's Yixing City is the test point) and rice in north china (Heilongjiang Province's Shuangcheng City is the test point), helps this fertilizer and promotes the use of in China.
Embodiment
Embodiment one: this embodiment is based on the preparation of the compound paddy rice special bio fertilizer of bush mycorrhizal fungi; Carry out according to the following steps: the preparation of, growing fortifier surely: 1kg butch clover seed adds 1.5kg water and 0.001kg ammonium molybdate; Soak behind the 12h with distilled water flushing 3 ~ 5 times, promptly get after control is dried;
Two, the pre-treatment of bush mycorrhizal fungi microbial inoculum: butch clover root in the bush mycorrhizal fungi microbial inoculum is shredded to 1 ~ 2cm, the matrix in the bush mycorrhizal fungi microbial inoculum was smashed 10 order soil sieves, be the bush mycorrhizal fungi microbial inoculum of handling well behind the two mixing;
Three, with the bush mycorrhizal fungi microbial inoculum of handling well, rice seedling-growth soil regulator with grow fortifier surely by (1500 ~ 2500): (300 ~ 600): 1 volume ratio mixing, promptly accomplish preparation based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi.
The butch clover seed is available from Shenyang side slope plant biological bag factory (telephone number: 024-89150311 in this embodiment step 1; Contact address: Shujiatun District camellia street, Shenyang City).
Rice seedling-growth soil regulator is available from Heilungkiang Wan Fengde fertilizer ltd (telephone number 0454-6223777, contact address: industrial park, Huanan, Heilungkiang) in this embodiment step 3.
Embodiment two: what this embodiment and embodiment one were different is the preparation of bush mycorrhizal fungi microbial inoculum in the step 3; Carry out according to the following steps: one, field soil, river sand and vermiculite are crossed 10 order soil sieves respectively; Then with the 2:5:3 mixing by volume of field soil, river sand and vermiculite; As matrix, matrix in 121 ℃ of following high pressure steam sterilization 2h, is cleaned flowerpot and dried the back with 0.1% potassium permanganate solution immersion 30min; Two, the butch clover seed being used volumetric concentration is 75% alcohol-pickled 10min, uses distilled water flushing then 3 times, in 28 ℃ of vernalization; Three, in each flowerpot, pack into the earlier matrix of 6kg sterilization; Spray sterilized water to water and ooze out from Base of flowerpot, bush mycorrhizal fungi is inoculated in the matrix, inoculum size is 20 spores of each flowerpot; Evenly sprinkle the butch clover seed that has germinateed then, cover matrix 1kg; Four, the every basin in 1 week back of emerging stays seedling 30 strains, continues to cultivate for 6 weeks, removes the butch clover over-ground part then, reclaims matrix and butch clover root, and natural air drying promptly get the bush mycorrhizal fungi microbial inoculum, is positioned over ventilations, cool place, drying and locates preservation;
Wherein field soil pH value is 7.7 in the step 1, and total nitrogen content is 6.75g/kg, and content of tatal phosphorus is 13.07g/kg; The butch clover seed is available from Shenyang side slope plant biological bag factory in the step 2; Bush mycorrhizal fungi specifically is to adopt Glomus mosseae HDSF1 in the step 3, is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center, and deposit number is CGMCC No.3012.
Embodiment three: this embodiment and embodiment one are different is bush mycorrhizal fungi microbial inoculum, the rice seedling-growth soil regulator of handling well in the step 3 and grows the volume ratio mixing of fortifier by 2000:450:1 surely.Other step and parameter are identical with embodiment one.
Embodiment four: this embodiment be described in the embodiment one based on the application of the compound paddy rice special bio fertilizer of bush mycorrhizal fungi, carry out according to the following steps:
One, field soil is tiled in the seedling dish, thickness is 1.5cm, adds the compound paddy rice special bio fertilizer 250g based on bush mycorrhizal fungi, processes the seedling bed;
Two, water spray is sprinkled into the 40g percentage of germination uniformly and reaches 95% ~ 98% rice paddy seed to drenched fully on the seedling bed, and rice paddy seed three faces bury, seedling bed earthing then, thickness is 0.5cm, by the rice shoot control measures of routine cultivated 30 days the mycorhiza seedling;
Three, be 36m at area
2The rice field in establish the wide guard rows of 1m; The border is done to cut off with the geomembrane of high 80cm and is handled; Wherein 50cm is underground hydrology blocking-up; 30cm is hydrology blocking-up on the ground, manages the rice field by the high yield liquid manure mode of routine, promptly accomplishes the application based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi.
Embodiment: experimental group and control group, each is provided with 10 repetitions, and each is handled the minizone and is divided into 50cm, isolates with the fragment of brick of parcel plastics film around the sub-district.
One, field soil is tiled in the seedling dish, thickness is 1.5cm, adds the compound paddy rice special bio fertilizer 250g based on bush mycorrhizal fungi, processes the seedling bed;
Two, water spray is sprinkled into the 40g percentage of germination uniformly and reaches 95% ~ 98% rice paddy seed to drenched fully on the seedling bed, and rice paddy seed three faces bury, seedling bed earthing then, thickness is 0.5cm, by the rice shoot control measures of routine cultivated 30 days the mycorhiza seedling;
Three, be 36m at area
2The rice field in establish the wide guard rows of 1m; The border is done to cut off with the geomembrane of high 80cm and is handled; Wherein 50cm is underground hydrology blocking-up, and 30cm is hydrology blocking-up on the ground, manages the rice field by the high yield liquid manure mode of routine; Promptly accomplish application based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi, with this as experimental group (being M).
Do not apply compound paddy rice special bio fertilizer based on bush mycorrhizal fungi as control group (being CK), add the 50g rice seedling-growth soil regulator, application method is the same, must contrast seedling.
Test results shown in Table 1, the use of arbuscular mycorrhizal fungi based on complex dedicated bio-fertilizer for rice seedlings, seedlings with Nie rate, stalk wide organization plumpness large seedlings are growing neat and Seeding rate increased; Table 2 shows that the use of arbuscular mycorrhizal fungi based on complex dedicated bio-fertilizer for rice seedlings, seedling root system, the specific performance of the longest root length, total root number, white roots and root dry weight, root rooting capacity enhancement; Table 3 shows arbuscular mycorrhizal fungi based composite bio-fertilizer for Rice rice seedling weeds can reduce the type and quantity, reducing the rice seedlings to light, space and nutrients competitive pressures; Table 4 shows that based on mycorrhizal fungal compound Rice dedicated bio-fertilizer is applied to the seedling though, but Honda has a higher stage of fertilizer, the specific performance of rice to produce more effective tillers, photosynthetic rate increased, a larger proportion of seed material distribution and, ultimately, more high economic yield.
Table 1 is based on the compound paddy rice special bio fertilizer of the bush mycorrhizal fungi growth-promoting functions to the rice seedling over-ground part
Table 2 is based on the compound paddy rice special bio fertilizer of the bush mycorrhizal fungi growth-promoting functions to the rice seedling underground part
Table 3 is based on the compound paddy rice special bio fertilizer of the bush mycorrhizal fungi restraining effect to seedling bed weeds
Table 4 is based on the compound paddy rice special bio fertilizer of the bush mycorrhizal fungi growth-promoting functions to the Honda paddy rice
Claims (4)
1. based on the preparation of the compound paddy rice special bio fertilizer of bush mycorrhizal fungi; It is characterized in that it carries out according to the following steps: the preparation of, growing fortifier surely: 1kg butch clover seed adds 1.5kg water and 0.001kg ammonium molybdate; Soak behind the 12h with distilled water flushing 3 ~ 5 times, control is dried then;
Two, the pre-treatment of bush mycorrhizal fungi microbial inoculum: butch clover root in the bush mycorrhizal fungi microbial inoculum is shredded to 1 ~ 2cm, the matrix in the bush mycorrhizal fungi microbial inoculum was smashed 10 order soil sieves, be the bush mycorrhizal fungi microbial inoculum of handling well behind the two mixing;
Three, with the bush mycorrhizal fungi microbial inoculum of handling well, rice seedling-growth soil regulator with grow fortifier surely by (1500 ~ 2500): (300 ~ 600): 1 volume ratio mixing, promptly accomplish preparation based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi.
2. the preparation of the compound paddy rice special bio fertilizer based on bush mycorrhizal fungi according to claim 1; It is characterized in that the preparation of bush mycorrhizal fungi microbial inoculum in the step 3; Carry out according to the following steps: one, field soil, river sand and vermiculite are crossed 10 order soil sieves respectively, then with the 2:5:3 mixing by volume of field soil, river sand and vermiculite, as matrix; Matrix in 121 ℃ of following high pressure steam sterilization 2h, is cleaned flowerpot and dried the back with 0.1% potassium permanganate solution immersion 30min; Two, the butch clover seed being used volumetric concentration is 75% alcohol-pickled 10min, uses distilled water flushing then 3 times, in 28 ℃ of vernalization; Three, in each flowerpot, pack into the earlier matrix of 6kg sterilization; Spray sterilized water to water and ooze out from Base of flowerpot, bush mycorrhizal fungi is inoculated in the matrix, inoculum size is 20 spores of each flowerpot; Evenly sprinkle the butch clover seed that has germinateed then, cover matrix 1kg; Four, the every basin in 1 week back of emerging stays seedling 30 strains, continues to cultivate for 6 weeks, removes the butch clover over-ground part then, reclaims matrix and butch clover root, and natural air drying promptly get the bush mycorrhizal fungi microbial inoculum, is positioned over ventilations, cool place, drying and locates preservation;
Wherein field soil pH value is 7.7 in the step 1, and total nitrogen content is 6.75g/kg, and content of tatal phosphorus is 13.07g/kg; The butch clover seed is available from Shenyang side slope plant biological bag factory in the step 2; Bush mycorrhizal fungi specifically is to adopt Glomus mosseae HDSF1 in the step 3, is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center, and deposit number is CGMCCNo.3012.
3. the preparation of the compound paddy rice special bio fertilizer based on bush mycorrhizal fungi according to claim 1 is characterized in that bush mycorrhizal fungi microbial inoculum, the rice seedling-growth soil regulator of handling well in the step 3 and grows the volume ratio mixing of fortifier by 2000:450:1 surely.
4. like the prepared application of claim 1, it is characterized in that it carries out according to the following steps based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi:
One, field soil is tiled in the seedling dish, thickness is 1.5cm, adds the compound paddy rice special bio fertilizer 250g based on bush mycorrhizal fungi, processes the seedling bed;
Two, water spray is sprinkled into the 40g percentage of germination uniformly and reaches 95% ~ 98% rice paddy seed to drenched fully on the seedling bed, and rice paddy seed three faces bury, seedling bed earthing then, thickness is 0.5cm, by the rice shoot control measures of routine cultivated 30 days the mycorhiza seedling;
Three, be 36m at area
2The rice field in establish the wide guard rows of 1m; The border is done to cut off with the geomembrane of high 80cm and is handled; Wherein 50cm is underground hydrology blocking-up; 30cm is hydrology blocking-up on the ground, manages the rice field by the high yield liquid manure mode of routine, promptly accomplishes the application based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi.
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| CN103314676A (en) * | 2013-04-12 | 2013-09-25 | 宋福强 | Method for carrying out industrialized production on arbuscular mycorrhizal fungi agent |
| CN103814953A (en) * | 2014-01-11 | 2014-05-28 | 哈尔滨工业大学宜兴环保研究院 | Fungal field weed inhibitor and preparation method thereof |
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| CN105085057A (en) * | 2015-08-21 | 2015-11-25 | 南京翠京元生物科技有限公司 | Biological type organic-inorganic compound fertilizer and method for preparing same |
| CN105145059A (en) * | 2015-08-21 | 2015-12-16 | 南京翠京元生物科技有限公司 | Method for cultivating paddy rice using arbuscular mycorrhizal fungi |
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| CN113243157A (en) * | 2021-05-07 | 2021-08-13 | 黑龙江大学 | Method for raising rice seedlings by combining arbuscular mycorrhizal fungi and Piriformospora indica |
| CN114303811A (en) * | 2021-11-30 | 2022-04-12 | 江西省水利科学院 | Method for reducing non-point source pollution of phosphorus in navel orange garden based on AM fungus |
| CN115067153A (en) * | 2022-05-17 | 2022-09-20 | 西安科技大学 | Method for enhancing tensile damage resistance of root system and shear resistance of root-soil complex by combining DSE and AMF |
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| CN114303811A (en) * | 2021-11-30 | 2022-04-12 | 江西省水利科学院 | Method for reducing non-point source pollution of phosphorus in navel orange garden based on AM fungus |
| CN115067153A (en) * | 2022-05-17 | 2022-09-20 | 西安科技大学 | Method for enhancing tensile damage resistance of root system and shear resistance of root-soil complex by combining DSE and AMF |
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