CN102718599B - Preparation of compound rice dedicated bio-fertilizer based on arbuscular mycorrhizal fungi and application thereof - Google Patents
Preparation of compound rice dedicated bio-fertilizer based on arbuscular mycorrhizal fungi and application thereof Download PDFInfo
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- CN102718599B CN102718599B CN2012102290442A CN201210229044A CN102718599B CN 102718599 B CN102718599 B CN 102718599B CN 2012102290442 A CN2012102290442 A CN 2012102290442A CN 201210229044 A CN201210229044 A CN 201210229044A CN 102718599 B CN102718599 B CN 102718599B
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Abstract
The invention discloses a preparation of compound rice dedicated bio-fertilizer based on arbuscular mycorrhizal fungi and an application thereof, and relates to the preparation and the application of bio-fertilizer. The compound rice dedicated bio-fertilizer aims to solve the problem that rice can not be infected by the arbuscular mycorrhizal fungi under the aquatic condition at present and is difficult to effectively form mutualistic symbiosis, and therefore the rice can not obtain mineral nutrition from an arbuscular mycorrhizal fungi-crop symbiotic system. The method comprises the following steps of: preparing a colonized reinforcer; pre-processing arbuscular mycorrhizal fungi fungicide; and evenly mixing the processed arbuscular mycorrhizal fungi fungicide, rice shoots growth promoterand the colonized reinforce. The compound rice dedicated bio-fertilizer can be prepared into a seedling bed, culturing the mycorrhiza seedling and managing a rice field. According to the compound rice dedicated bio-fertilizer disclosed by the invention, the arbuscular mycorrhizal fungi-crop symbiotic system with a high infection rate and stable performance can be built in the seedling stage of the rice, and the symbiotic system can successfully live through the transplanted field flooding environment. The biological bactericide activity loss problem brought by flooding intimidation can be effectively solved, mineral nutrition is continuously provided for the rice, the growth situation of the rice is improved, the fertilizer application amount in the field is reduced, and the rice production cost can be effectively lowered.
Description
Technical field
The present invention relates to preparation and the application thereof of paddy rice special bio fertilizer.
Background technology
Nitrogen and phosphorus are the essential macroelement of paddy growth, and nitrogen phosphorus shortage can cause that Crop development is bad, finally causes declining to a great extent of yield and quality of rice.Significant contribution has been made in using as the growth of today whole world paddy rice of chemical fertilizer.Yet the rice yield that chemical fertilizer is depended on unduly increases to sacrifice ecotope as cost.Paddy rice is very low to the utilization ratio of chemical fertilizer, and unabsorbed nutrition then causes environmental pollution by modes such as seepage, rainwash, denitrifications.In addition, the greenhouse gases of generation are used in nitrogen fertilizer production and farmland thereof, such as CO
2, N
2O and NO
x, also more and more large on the impact of Global climate change.Simultaneously, the excessive exploitation of phosphorus ore has also caused the exhaustion of phosphate rock resource.Rice biological fertilizer has increases soil fertility, and promotes Soil Mineral Nutrition to discharge, and reduces disease and pest, improves the functions such as rice quality and raising output.Yet the bio-feritlizer that is used at present Rice Production can only be worked in the non-waterflooding phase of paddy growth by the water logging condition restriction, the fertilizer efficiency time length is short, effect stability is poor, manufacture craft is loaded down with trivial details, store method is complicated, function singleness is difficult to effective application and spread in rice on honda production.
Summary of the invention
The present invention seeks to be infected by bush mycorrhizal fungi at aquatic conditions in order to solve present paddy rice, be difficult to effectively form symbiosis, cause paddy rice can not obtain from arbuscular mycorrhizal fungus-do cogeneration system the problem of mineral nutrition, and preparation and application thereof based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi are provided.
Preparation based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi, carry out according to the following steps: the preparation of, surely growing fortifier: 1kg butch clover seed adds 1.5kg water and 0.001kg ammonium molybdate, use distilled water flushing 3 ~ 5 times after soaking 12h, then control is done;
Two, the pre-treatment of bush mycorrhizal fungi preparation: butch clover root in the bush mycorrhizal fungi preparation is shredded to 1 ~ 2cm, the matrix in the bush mycorrhizal fungi preparation was smashed 10 order soil sieves, be the bush mycorrhizal fungi preparation of handling well behind the two mixing;
Three, with bush mycorrhizal fungi preparation, the rice seedling-growth soil regulator handled well with surely grow fortifier by (1500 ~ 2500): (300 ~ 600): 1 volume ratio mixing, namely finish the preparation based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi.
The application of above-mentioned compound paddy rice special bio fertilizer based on bush mycorrhizal fungi, carry out according to the following steps:
One, field soil is tiled in seedling-cultivation plate, thickness is 1.5cm, adds the compound paddy rice special bio fertilizer 250g based on bush mycorrhizal fungi, makes the seedling bed;
Two, water spray is sprinkled into uniformly the 40g percentage of germination and reaches 95% ~ 98% rice paddy seed to fully drenched on the seedling bed, and rice paddy seed three faces bury, seedling bed earthing then, and thickness is 0.5cm, rice shoot control measures were routinely cultivated 30 days to get Va Mycorrhiza Seedling;
Three, be 36m at area
2The rice field in establish the wide guard rows of 1m; the border is done to cut off with the geomembrane of high 80cm and is processed; wherein 50cm is the underground hydrological blocking-up; 30cm is hydrology blocking-up on the ground; high yield liquid manure mode is routinely managed the rice field, namely finishes the application based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi.
The present invention is based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi, make paddy rice can set up the arbuscular mycorrhizal fungus of high infection rate, stable performance-do cogeneration system in the seedling phase.And this cogeneration system can be tided over the Honda water logging environment after the transplanting smoothly, effectively solve water logging and coerce the bacteria agent loss of activity problem of bringing, be continuously paddy rice mineral nutrition is provided, improve the upgrowth situation of paddy rice, reduce the amount of application of this Tanaka chemical fertilizer, alleviate the negative impact that chemical fertilizer causes surface water and groundwater.In addition, amount of application of the present invention is little, and the short of paddy rice come into force fruit obviously, can effectively reduce the cost of Rice Production.Production method of the present invention is simple simultaneously, is easy to preserve, and is fit to large-scale promotion application.
The present invention is based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi, the whole growth cycle of paddy rice is all had significant promoter action.At first, utilize the present invention to carry out raising rice seedlings, rice shoot is healthy and strong, and the wide ， Dai of side stem Nie leads high, and the root of hair ability is strong, and it is fast to turn green, and high yield characteristic is good.Simultaneously the present invention can reduce kind and the quantity of rice seedling bed weeds, for the growth of rice seedling provides good ecotope.In addition, apply this fertilizer in the rice seedling bed stage and can promote growing of Honda stage paddy rice, be embodied in and improve Rice Photosynthesis efficient, improve the paddy rice dispensed materials, improve rice yield.The compound paddy rice special bio fertilizer that the present invention is based on bush mycorrhizal fungi all has the good short fruit of coming into force to south rice (Jiangsu Province's Yixing City is the test point) and rice in north china (Heilongjiang Province's Shuangcheng City is the test point), is conducive to this fertilizer and promotes the use of in China.
Embodiment
Embodiment one: present embodiment is based on the preparation of the compound paddy rice special bio fertilizer of bush mycorrhizal fungi, carry out according to the following steps: the preparation of, surely growing fortifier: 1kg butch clover seed adds 1.5kg water and 0.001kg ammonium molybdate, soak behind the 12h with distilled water flushing 3 ~ 5 times, after control is done and get final product;
Two, the pre-treatment of bush mycorrhizal fungi preparation: butch clover root in the bush mycorrhizal fungi preparation is shredded to 1 ~ 2cm, the matrix in the bush mycorrhizal fungi preparation was smashed 10 order soil sieves, be the bush mycorrhizal fungi preparation of handling well behind the two mixing;
Three, with bush mycorrhizal fungi preparation, the rice seedling-growth soil regulator handled well with surely grow fortifier by (1500 ~ 2500): (300 ~ 600): 1 volume ratio mixing, namely finish the preparation based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi.
The butch clover seed is available from Shenyang side slope plant biological bag factory (telephone number: 024-89150311 in the present embodiment step 1; Contact address: Shujiatun District camellia street, Shenyang City).
Rice seedling-growth soil regulator is available from Heilungkiang Wan Fengde fertilizer company limited (telephone number 0454-6223777, contact address: industrial park, Huanan, Heilungkiang) in the present embodiment step 3.
Embodiment two: what present embodiment and embodiment one were different is the preparation of bush mycorrhizal fungi preparation in the step 3, carry out according to the following steps: one, field soil, river sand and vermiculite are crossed respectively 10 order soil sieves, then with the by volume 2:5:3 mixing of field soil, river sand and vermiculite, as matrix, matrix in 121 ℃ of lower high pressure steam sterilization 2h, is cleaned the potassium permanganate solution that dries rear usefulness 0.1% with flowerpot and soaked 30min; Two, with butch clover seed volumetric concentration be 75% alcohol-pickled 10min, then use distilled water flushing 3 times, in 28 ℃ of vernalization; Three, in each flowerpot, pack into the first matrix of 6kg sterilization, spray sterilized water to water and ooze out from Base of flowerpot, bush mycorrhizal fungi is inoculated in the matrix, inoculum size is 20 spores of each flowerpot, then evenly sprinkle the butch clover seed that has germinateed, cover matrix 1kg; Four, emerge that every basin stays seedling 30 strains after 1 week, continue to cultivate for 6 weeks, then remove the butch clover over-ground part, reclaim matrix and butch clover root, natural air drying namely gets bush mycorrhizal fungi preparation, is positioned over ventilations, cool place, dryly locates preservation;
Wherein field soil pH value is 7.7 in the step 1, and total nitrogen content is 6.75g/kg, and content of tatal phosphorus is 13.07g/kg; The butch clover seed is available from Shenyang side slope plant biological bag factory in the step 2; Bush mycorrhizal fungi specifically adopts Glomus mosseae HDSF1 in the step 3, is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center, and deposit number is CGMCC No.3012.
Embodiment three: present embodiment and embodiment one are different is bush mycorrhizal fungi preparation, the rice seedling-growth soil regulator of handling well in the step 3 and surely grows fortifier by the volume ratio mixing of 2000:450:1.Other step and parameter are identical with embodiment one.
Embodiment four: present embodiment be described in the embodiment one based on the application of the compound paddy rice special bio fertilizer of bush mycorrhizal fungi, carry out according to the following steps:
One, field soil is tiled in seedling-cultivation plate, thickness is 1.5cm, adds the compound paddy rice special bio fertilizer 250g based on bush mycorrhizal fungi, makes the seedling bed;
Two, water spray is sprinkled into uniformly the 40g percentage of germination and reaches 95% ~ 98% rice paddy seed to fully drenched on the seedling bed, and rice paddy seed three faces bury, seedling bed earthing then, and thickness is 0.5cm, rice shoot control measures were routinely cultivated 30 days to get Va Mycorrhiza Seedling;
Three, be 36m at area
2The rice field in establish the wide guard rows of 1m; the border is done to cut off with the geomembrane of high 80cm and is processed; wherein 50cm is the underground hydrological blocking-up; 30cm is hydrology blocking-up on the ground; high yield liquid manure mode is routinely managed the rice field, namely finishes the application based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi.
Embodiment: experimental group and control group, each arranges 10 repetitions, and each is processed the minizone and is divided into 50cm, isolates with the fragment of brick of parcel plastics film around the residential quarter.
One, field soil is tiled in seedling-cultivation plate, thickness is 1.5cm, adds the compound paddy rice special bio fertilizer 250g based on bush mycorrhizal fungi, makes the seedling bed;
Two, water spray is sprinkled into uniformly the 40g percentage of germination and reaches 95% ~ 98% rice paddy seed to fully drenched on the seedling bed, and rice paddy seed three faces bury, seedling bed earthing then, and thickness is 0.5cm, rice shoot control measures were routinely cultivated 30 days to get Va Mycorrhiza Seedling;
Three, be 36m at area
2The rice field in establish the wide guard rows of 1m; the border is done to cut off with the geomembrane of high 80cm and is processed; wherein 50cm is the underground hydrological blocking-up; 30cm is hydrology blocking-up on the ground; high yield liquid manure mode is routinely managed the rice field; namely finish the application based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi, with this as experimental group (being M).
Do not apply based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi and organize in contrast (being CK), add the 50g rice seedling-growth soil regulator, application method is the same, gets the contrast seedling.
Detected result is as shown in table 1, utilizes and grows seedlings based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi, and rice shoot band Nie leads high, and stem foot is wide, organizes circularity large, and the rice shoot growing way is neat, and adult seedling rate improves; Table 2 shows to utilize grows seedlings based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi, the rice shoot well developed root system, and being embodied in long, the total radical of the longest root, white root and weight of root system increases, and root system root of hair ability strengthens; Table 3 illustrates kind and the quantity that can reduce the rice seedling bed weeds based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi, has reduced the competitive pressure to illumination, space and nutrition of rice seedling; Although table 4 shows the compound paddy rice special bio fertilizer based on bush mycorrhizal fungi and is applied to the rice seedling bed, but still has higher fertilizer efficiency in the Honda stage, be embodied in and make paddy rice produce more effective tillering, photosynthetic rate strengthens, seed dispensed materials ratio increases, the higher economic yield of final acquisition.
Table 1 is based on the compound paddy rice special bio fertilizer of the bush mycorrhizal fungi growth-promoting functions to the rice seedling over-ground part
Table 2 is based on the compound paddy rice special bio fertilizer of the bush mycorrhizal fungi growth-promoting functions to the rice seedling underground part
Table 3 is based on the compound paddy rice special bio fertilizer of the bush mycorrhizal fungi restraining effect to seedling bed weeds
Table 4 is based on the compound paddy rice special bio fertilizer of the bush mycorrhizal fungi growth-promoting functions to the Honda paddy rice
Claims (3)
1. based on the preparation of the compound paddy rice special bio fertilizer of bush mycorrhizal fungi, it is characterized in that it carries out according to the following steps:
One, surely grow the preparation of fortifier: 1kg butch clover seed adds 1.5kg water and 0.001kg ammonium molybdate, uses distilled water flushing 3～5 times behind the immersion 12h, and then control is done;
Two, the pre-treatment of bush mycorrhizal fungi preparation: butch clover root in the bush mycorrhizal fungi preparation is shredded to 1～2cm, the matrix in the bush mycorrhizal fungi preparation was smashed 10 order soil sieves, be the bush mycorrhizal fungi preparation of handling well behind the two mixing;
Three, with bush mycorrhizal fungi preparation, the rice seedling-growth soil regulator handled well with surely grow fortifier by (1500～2500): (300～600): 1 volume ratio mixing, namely finish the preparation based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi;
The wherein preparation of bush mycorrhizal fungi preparation in the step 2, carry out according to the following steps: a, field soil, river sand and vermiculite are crossed respectively 10 order soil sieves, then with the by volume 2:5:3 mixing of field soil, river sand and vermiculite, as matrix, matrix in 121 ℃ of lower high pressure steam sterilization 2h, is cleaned the potassium permanganate solution that dries rear usefulness 0.1% with flowerpot and soaked 30min; B, be 75% alcohol-pickled 10min with butch clover seed volumetric concentration, then use distilled water flushing 3 times, in 28 ℃ of vernalization; The matrix of c, the 6kg that in each flowerpot, packs into first sterilization, spray sterilized water to water and ooze out from Base of flowerpot, bush mycorrhizal fungi is inoculated in the matrix, inoculum size is 20 spores of each flowerpot, then evenly sprinkle the butch clover seed that has germinateed, cover matrix 1kg; D, every basin stays seedling 30 strains after 1 week of emerging, and continue to cultivate for 6 weeks, then removes the butch clover over-ground part, reclaims matrix and butch clover root, and natural air drying namely gets bush mycorrhizal fungi preparation, is positioned over ventilations, cool place, dryly locates preservation;
Wherein field soil pH value is 7.7 among the step a, and total nitrogen content is 6.75g/kg, and content of tatal phosphorus is 13.07g/kg; The butch clover seed is available from Shenyang side slope plant biological bag factory among the step b; Bush mycorrhizal fungi specifically adopts Glomus mosseae HDSF1 among the step c, is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center, and deposit number is CGMCCNo.3012.
2. the preparation of the compound paddy rice special bio fertilizer based on bush mycorrhizal fungi according to claim 1 is characterized in that bush mycorrhizal fungi preparation, the rice seedling-growth soil regulator of handling well in the step 3 and surely grows fortifier by the volume ratio mixing of 2000:450:1.
3. such as the prepared application based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi of claim 1, it is characterized in that it carries out according to the following steps:
One, field soil is tiled in seedling-cultivation plate, thickness is 1.5cm, adds the compound paddy rice special bio fertilizer 250g based on bush mycorrhizal fungi, makes the seedling bed;
Two, water spray is sprinkled into uniformly the 40g percentage of germination and reaches 95%～98% rice paddy seed to fully drenched on the seedling bed, and rice paddy seed three faces bury, seedling bed earthing then, and thickness is 0.5cm, rice shoot control measures were routinely cultivated 30 days to get Va Mycorrhiza Seedling;
Three, be 36m at area
2The rice field in establish the wide guard rows of 1m; the border is done to cut off with the geomembrane of high 80cm and is processed; wherein 50cm is the underground hydrological blocking-up; 30cm is hydrology blocking-up on the ground; high yield liquid manure mode is routinely managed the rice field, namely finishes the application based on the compound paddy rice special bio fertilizer of bush mycorrhizal fungi.
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| CN113243157B (en) * | 2021-05-07 | 2023-05-09 | 黑龙江大学 | Method for raising rice seedlings by combining arbuscular mycorrhizal fungi and Pityrosporum indicum |
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