CN102796817A - Method for rapidly detecting mutation of KRAS gene - Google Patents
Method for rapidly detecting mutation of KRAS gene Download PDFInfo
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- CN102796817A CN102796817A CN2012102337504A CN201210233750A CN102796817A CN 102796817 A CN102796817 A CN 102796817A CN 2012102337504 A CN2012102337504 A CN 2012102337504A CN 201210233750 A CN201210233750 A CN 201210233750A CN 102796817 A CN102796817 A CN 102796817A
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- kras
- primer
- molecular beacon
- pcr
- beacon probe
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CN201210233750.4A CN102796817B (en) | 2012-07-06 | 2012-07-06 | Method for rapidly detecting mutation of KRAS gene |
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CN201210233750.4A CN102796817B (en) | 2012-07-06 | 2012-07-06 | Method for rapidly detecting mutation of KRAS gene |
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CN102796817A true CN102796817A (en) | 2012-11-28 |
CN102796817B CN102796817B (en) | 2014-06-25 |
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103088137A (en) * | 2013-01-18 | 2013-05-08 | 陕西北美基因股份有限公司 | Method for accurately detecting KRAS gene mutation by use of probe melting technology |
CN103103269A (en) * | 2013-01-18 | 2013-05-15 | 陕西北美基因股份有限公司 | Method for detecting BRAF (block repeat active flag) gene mutation accurately based on probe fusion technology |
CN104651529A (en) * | 2015-03-24 | 2015-05-27 | 厦门大学 | Method for detecting deletion mutation of nucleic acid molecule |
CN106834520A (en) * | 2017-03-27 | 2017-06-13 | 杭州迪安生物技术有限公司 | A kind of kit of utilization molecular beacon melting curve technical appraisement bacterium and its application |
CN107034277A (en) * | 2017-04-27 | 2017-08-11 | 厦门大学 | A kind of method for detecting low abundance gene mutation |
CN107385016A (en) * | 2017-06-14 | 2017-11-24 | 苏州承美生物科技有限公司 | A kind of molecular beacon probe and kit for detecting KRAS gene mutation |
CN112063701A (en) * | 2020-10-13 | 2020-12-11 | 苏州中科先进技术研究院有限公司 | Nucleic acid composition for detecting KRAS gene mutation, kit and detection method thereof |
CN116426617A (en) * | 2023-04-25 | 2023-07-14 | 无锡市人民医院 | High-sensitivity mutation detection system based on hairpin structure and enzyme digestion mechanism and application |
Citations (3)
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US6090552A (en) * | 1996-07-16 | 2000-07-18 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
CN102115792A (en) * | 2010-12-20 | 2011-07-06 | 苏州科贝生物技术有限公司 | Method and kit for detecting KRAS gene mutations in human colon and rectum cancers |
CN102321765A (en) * | 2011-09-08 | 2012-01-18 | 厦门基科生物科技有限公司 | Real-time fluorescent PCR (polymerase chain reaction) method and application |
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2012
- 2012-07-06 CN CN201210233750.4A patent/CN102796817B/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6090552A (en) * | 1996-07-16 | 2000-07-18 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
CN102115792A (en) * | 2010-12-20 | 2011-07-06 | 苏州科贝生物技术有限公司 | Method and kit for detecting KRAS gene mutations in human colon and rectum cancers |
CN102321765A (en) * | 2011-09-08 | 2012-01-18 | 厦门基科生物科技有限公司 | Real-time fluorescent PCR (polymerase chain reaction) method and application |
Non-Patent Citations (2)
Title |
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于黎娟: "分子信标的研究现状及应用", 《科技信息》, no. 21, 30 July 2010 (2010-07-30) * |
余丽丽: "新型核酸分子荧光探针-分子信标的研究进展", 《化学与生物工程》, vol. 29, no. 4, 30 April 2012 (2012-04-30) * |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103088137A (en) * | 2013-01-18 | 2013-05-08 | 陕西北美基因股份有限公司 | Method for accurately detecting KRAS gene mutation by use of probe melting technology |
CN103103269A (en) * | 2013-01-18 | 2013-05-15 | 陕西北美基因股份有限公司 | Method for detecting BRAF (block repeat active flag) gene mutation accurately based on probe fusion technology |
CN103088137B (en) * | 2013-01-18 | 2014-11-05 | 陕西北美基因股份有限公司 | Method for accurately detecting KRAS gene mutation by use of probe melting technology |
CN103103269B (en) * | 2013-01-18 | 2015-07-15 | 陕西佰美基因股份有限公司 | Method for detecting BRAF (block repeat active flag) gene mutation accurately based on probe fusion technology |
CN104651529A (en) * | 2015-03-24 | 2015-05-27 | 厦门大学 | Method for detecting deletion mutation of nucleic acid molecule |
CN106834520A (en) * | 2017-03-27 | 2017-06-13 | 杭州迪安生物技术有限公司 | A kind of kit of utilization molecular beacon melting curve technical appraisement bacterium and its application |
CN106834520B (en) * | 2017-03-27 | 2020-12-18 | 杭州迪安生物技术有限公司 | Kit for identifying bacteria by using molecular beacon-melting curve technology and application thereof |
CN107034277A (en) * | 2017-04-27 | 2017-08-11 | 厦门大学 | A kind of method for detecting low abundance gene mutation |
CN107385016A (en) * | 2017-06-14 | 2017-11-24 | 苏州承美生物科技有限公司 | A kind of molecular beacon probe and kit for detecting KRAS gene mutation |
CN112063701A (en) * | 2020-10-13 | 2020-12-11 | 苏州中科先进技术研究院有限公司 | Nucleic acid composition for detecting KRAS gene mutation, kit and detection method thereof |
CN116426617A (en) * | 2023-04-25 | 2023-07-14 | 无锡市人民医院 | High-sensitivity mutation detection system based on hairpin structure and enzyme digestion mechanism and application |
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Publication number | Publication date |
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CN102796817B (en) | 2014-06-25 |
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Owner name: SHAANXI BAIMEI GENE CO., LTD. Free format text: FORMER NAME: SHAANXI LIFEGEN CO., LTD. |
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Address after: 710069 Shaanxi city of Xi'an province Taibai Road No. 229 Patentee after: Shaanxi Baimei Gene Co., Ltd. Address before: 710069 Shaanxi city of Xi'an province Taibai Road No. 229 Patentee before: Shaanxi Beimei Gene Co., Ltd. |
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Owner name: SHAANXI BAIMEI MEDICAL EXAMINATION CO., LTD. Free format text: FORMER OWNER: SHAANXI BAIMEI GENE CO., LTD. Effective date: 20150827 |
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Effective date of registration: 20150827 Address after: 710116 Shaanxi city of Xi'an Province East Metro Symphony new West Ham Red Road and the southwest corner of a Keyuan Road cross port collaborative innovation R & D pilot Building No. 8 N402 Patentee after: Shaanxi Bai Mei Medical Inspection Co., Ltd. Address before: 710069 Shaanxi city of Xi'an province Taibai Road No. 229 Patentee before: Shaanxi Baimei Gene Co., Ltd. |