CN103439386A - Preparation method of biological sensing electrode - Google Patents
Preparation method of biological sensing electrode Download PDFInfo
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- CN103439386A CN103439386A CN2013103223100A CN201310322310A CN103439386A CN 103439386 A CN103439386 A CN 103439386A CN 2013103223100 A CN2013103223100 A CN 2013103223100A CN 201310322310 A CN201310322310 A CN 201310322310A CN 103439386 A CN103439386 A CN 103439386A
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Abstract
The invention discloses a preparation method of a biological sensing electrode, which comprises the following steps that an acupuncture needle is taken, polished, subjected to oil removal, flushed and dried; the two ends of a varnished wire are ground; a metal wire at one end of the acupuncture needle is welded with the tail end of the acupuncture needle; N,N-dimethylacetamide is placed in a beaker; polyether sulfone is added to an organic solvent and stirred; stirring is performed after polyether sulfone powder is dissolved; film forming liquid is generated; a part, welded with the varnished wire, of a needle handle of the acupuncture needle, and the varnished wire 3cm away from the welding part are soaked in the film forming liquid, and taken out; the soaked parts are placed in a condensation bath for film forming, taken out and air-dried; a needle body part of the acupuncture needle and partial needle handle close to the needle body part are coated with the film forming liquid; a part, from the needle handle to a needle tip, of the acupuncture needle is placed in the condensation bath for the film forming, taken out, cleaned, air-dried, placed in an oven, and taken out; the temperature of the electrode is adjusted to the room temperature; and the electrode is put in a refrigerator for standby. The preparation method is simple; the performance is stable; the micro signal collection efficiency is improved; and a toxic effect of a pollutant on a living organism can be revealed very well.
Description
Technical field the present invention relates to a kind of preparation method of electrode.
The background technology bio-sensing electrode has broad application prospects in fields such as clinical, food, environment, and its development, application are day by day rapid and extensive.Along with the raising of social industrialization degree, miscellaneous chemical substance constantly enters ecologic environment, ecologic environment and health is caused to day by day serious harm.Countries in the world have all given to show great attention to the biological safety of all kinds of chemical substances, pay much attention to test and the evaluation of its Environmental Toxicological.At present, the mensuration of environmental contaminants mainly contains physical chemistry measuring technology and biological test technology.Composition and the content of traditional physical chemistry technology energy quantitative test pollutant, but can not reflect directly, all sidedly the combined influence of noxious material to environment.The biological test technology mainly be take hydrobiont, sensitive plant and microorganism as study subject, and Chinese scholars has been carried out the toxotest research of each pollutant, then estimates the toxicity performance of pollutant.Microorganism has that body is little, population quantity is large, growth and breeding is fast, it is simple and convenient to preserve, to the swift advantage of environmental change, thereby take its bio-toxicity test that is study subject and can effectively make up the deficiency that the physical chemistry measuring technology exists.Although microorganism has the similar physicochemical characteristic of same higher mammal and enzyme mechanism to the intoxicating pollutant, but because the aspects such as the tolerance to the pollutant intoxicating and human body and other higher organisms there are differences, thereby the Ecotoxicology measuring technology can not reflect the harm of environmental contaminants to human body and other higher organisms preferably.Carry out the bio-toxicity test of hydrobiont and sensitive plant, toxicity that can qualitative sign pollutant, but whole process that can not reflected well pollutant intoxicating.With above-mentioned technology, compare, the bio-sensing measuring technology can be reflected in pollutant levels and distribution while changing to a certain extent, the respective change that the function of living organism occurs.
At present, Chinese scholars has been carried out fruitful work in environmental contaminants toxicity bio-sensing testing research field, take carbon nano-tube, aniline, pyrroles, styrene, acrylic acid, dopamine, metal phthalocyanine etc. is reagent, prepared the different kind organism sensing electrode, and by bioactive molecule embeddings such as DNA, laccase, acetylcholinesterases be fixed on electrode, adopt the measuring technologies such as cyclic voltammetry, Differential Pulse Voltammetry and AC impedance, measure the concentration of the intoxicating pollutants such as heavy metal, agricultural chemicals, estimated its environmental toxicity.Its weak point: preparation method's complexity, unstable properties, can not effectively improve the micro-signal collection efficiency, and can not disclose well the toxic action of pollutant to living organism.
Summary of the invention the purpose of this invention is to provide that a kind of preparation method is simple, stable performance, can effectively improve the micro-signal collection efficiency, and can disclose well the preparation method of pollutant to a kind of bio-sensing electrode of the toxic action of living organism.
Method for making of the present invention realizes according to the following steps:
(1) get the acupuncture needle that diameter of needle body is 0.18mm～0.25mm, by its polishing, oil removing, tap water and deionized water that the hot water that is 80 ℃ by temperature successively, temperature are room temperature are rinsed it well, then in the time of 100 ℃, with baking oven, it are dried;
(2) the enameled wire two ends sand papering that is diameter 0.2mm, until the tinsel that its two ends exposed length is 1cm～2cm, by the end soldering of the tinsel of one end and above-mentioned acupuncture needle;
(3) by 100 mass parts N, the N-dimethyl acetamide is placed in beaker, then the polyethersulfone of 15～18 mass parts is joined in the DMA organic solvent, controlling solution temperature is 80 ℃～90 ℃, uniform stirring, after the polyethersulfone powder fully dissolves, above-mentioned solution temperature is down to 60 ℃～70 ℃, and the temperature of maintenance solution is 60 ℃～70 ℃, continue to stir 3 hours, generate polyethersulfone film forming film forming liquid used;
(4) the position of the needle handle of acupuncture needle and enameled wire welding is immersed in above-mentioned film forming liquid, for guaranteeing electrode insulation, preferably the enameled wire apart from 3cm place, this welding position also is immersed in above-mentioned film forming liquid; After 30 seconds, it is taken out, and rapidly the position of soaking film forming liquid is placed in to condensation bath film forming, this condensation is bathed as the tap water after microfiltration membranes is processed, and water temperature is 40 ℃～50 ℃; After 20 minutes, by its taking-up, and dry;
(5) get the dried cotton ball wet by above-mentioned film forming immersion, clamp the cotton ball soaked with tweezers, above-mentioned film forming liquid evenly is coated in to the needle body position of acupuncture needle; For guaranteeing electrode insulation, preferably also should be applied the part needle handle near needle body position;
(6), after applying, the needle handle of acupuncture needle to the position of needle point is placed in fully, rapidly to condensation and bathes film forming; After 20 minutes, it is bathed and take out from condensation, with distilled water, it is cleaned and makes it naturally to dry;
(7) after electrode dries naturally, be placed in baking oven, the temperature of controlling baking oven is 60 ℃～80 ℃, after 3 hours, by its taking-up, treats that electrode temperature is chilled to normal temperature naturally, puts it in refrigerator, keeps 1 ℃～5 ℃, saves backup.
Preparation method of the present invention is simple, stable performance, can effectively improve the micro-signal collection efficiency, and can disclose well the preparation method of pollutant to a kind of bio-sensing electrode of the toxic action of living organism.
The accompanying drawing explanation
Fig. 1 is the structural representation of bio-sensing electrode;
Embodiment
In the schematic diagram of the present invention shown in Fig. 1, bio-sensing electrode mainly includes: acupuncture needle 1 and enameled wire 2.Wherein, tinsel soldering in the backshank of acupuncture needle and enameled wire.
Embodiment 1
Get the acupuncture needle that diameter of needle body is 0.18mm, by its polishing, oil removing, tap water and deionized water that the hot water that is 80 ℃ by temperature successively, temperature are room temperature are rinsed it well, then in the time of 100 ℃, with baking oven, it are dried, the enameled wire two ends sand papering that is diameter 0.2mm, until the tinsel of 1cm is exposed at its two ends, end soldering by the tinsel of one end and above-mentioned acupuncture needle, by 100gN, the N-dimethyl acetamide is placed in beaker, then the polyethersulfone of 15g is joined to N, in N-dimethyl acetamide organic solvent, controlling solution temperature is 80 ℃, uniform stirring, after the polyethersulfone powder fully dissolves, above-mentioned solution temperature is down to 60 ℃, and the temperature of maintenance solution is 60 ℃, continue to stir 3 hours, generate polyethersulfone film forming film forming liquid used, the position of the needle handle of acupuncture needle and enameled wire welding is immersed in above-mentioned film forming liquid, enameled wire apart from 3cm place, this welding position also is immersed in film forming liquid, after 30 seconds by its taking-up, and rapidly the position of soaking polyethersulfone film forming liquid is placed in to condensation bath film forming, this condensation is bathed as the tap water after microfiltration membranes is processed, water temperature is 40 ℃, after 20 minutes by its taking-up, and dry, get the dried cotton ball wet by above-mentioned polyethersulfone film forming immersion, clamp the cotton ball soaked with tweezers, polyethersulfone film forming liquid evenly is coated in to the needle body position of acupuncture needle, also should be applied the part needle handle near needle body position, after coating, the needle handle of acupuncture needle is complete to the position of needle point, be placed in rapidly condensation and bathe film forming, this condensation is bathed as the tap water after microfiltration membranes is processed, water temperature is 40 ℃, after 20 minutes, it is bathed and take out from condensation, with distilled water, it is cleaned and make it naturally to dry, after electrode dries naturally, be placed in baking oven, the temperature of controlling baking oven is 60 ℃, after 3 hours by its taking-up, treat that electrode temperature is chilled to normal temperature naturally, put it in refrigerator, keep 1 ℃～5 ℃, save backup.
Embodiment 2
Get the acupuncture needle that diameter of needle body is 0.2mm, by its polishing, oil removing, tap water and deionized water that the hot water that is 80 ℃ by temperature successively, temperature are room temperature are rinsed it well, then in the time of 100 ℃, with baking oven, it are dried, the enameled wire two ends sand papering that is diameter 0.2mm, until the tinsel of 1.5cm is exposed at its two ends, end soldering by the tinsel of one end and above-mentioned acupuncture needle, by 100gN, the N-dimethyl acetamide is placed in beaker, then the polyethersulfone of 16g is joined to N, in N-dimethyl acetamide organic solvent, controlling solution temperature is 85 ℃, uniform stirring, after the polyethersulfone powder fully dissolves, above-mentioned solution temperature is down to 65 ℃, and the temperature of maintenance solution is 65 ℃, continue to stir 3 hours, generate polyethersulfone film forming film forming liquid used, the position of the needle handle of acupuncture needle and enameled wire welding is immersed in above-mentioned polyethersulfone film forming liquid, enameled wire apart from 3cm place, this welding position also is immersed in polyethersulfone film forming liquid, after 30 seconds by its taking-up, and rapidly the position of soaking polyethersulfone film forming liquid is placed in to condensation bath film forming, this condensation is bathed as the tap water after microfiltration membranes is processed, water temperature is 45 ℃, after 20 minutes by its taking-up, and dry, get the dried cotton ball wet by above-mentioned polyethersulfone film forming immersion, clamp the cotton ball soaked with tweezers, polyethersulfone film forming liquid evenly is coated in to the needle body position of acupuncture needle, also should be applied the part needle handle near needle body position, after coating, the needle handle of acupuncture needle is complete to the position of needle point, be placed in rapidly condensation and bathe film forming, this condensation is bathed as the tap water after microfiltration membranes is processed, water temperature is 45 ℃, after 20 minutes, it is bathed and take out from condensation, with distilled water, it is cleaned and make it naturally to dry, after electrode dries naturally, be placed in baking oven, the temperature of controlling baking oven is 70 ℃, after 3 hours by its taking-up, treat that electrode temperature is chilled to normal temperature naturally, put it in refrigerator, keep 1 ℃～5 ℃, save backup.
Embodiment 3
Get the acupuncture needle that diameter of needle body is 0.22mm, by its polishing, oil removing, tap water and deionized water that the hot water that is 80 ℃ by temperature successively, temperature are room temperature are rinsed it well, then in the time of 100 ℃, with baking oven, it are dried, the enameled wire two ends sand papering that is diameter 0.2mm, until the tinsel of 2cm is exposed at its two ends, end soldering by the tinsel of one end and above-mentioned acupuncture needle, by 100gN, the N-dimethyl acetamide is placed in beaker, then the polyethersulfone of 17g is joined to N, in N-dimethyl acetamide organic solvent, controlling solution temperature is 90 ℃, uniform stirring, after the polyethersulfone powder fully dissolves, above-mentioned solution temperature is down to 70 ℃, and the temperature of maintenance solution is 70 ℃, continue to stir 3 hours, generate polyethersulfone film forming film forming liquid used, the position of the needle handle of acupuncture needle and enameled wire welding is immersed in above-mentioned polyethersulfone film forming liquid, enameled wire apart from 3cm place, this welding position also is immersed in polyethersulfone film forming liquid, after 30 seconds by its taking-up, and rapidly the position of soaking polyethersulfone film forming liquid is placed in to condensation bath film forming, this condensation is bathed as the tap water after microfiltration membranes is processed, water temperature is 40 ℃, after 30 minutes by its taking-up, and dry, get the dried cotton ball wet by above-mentioned polyethersulfone film forming immersion, clamp the cotton ball soaked with tweezers, polyethersulfone film forming liquid evenly is coated in to the needle body position of acupuncture needle, also should be applied the part needle handle near needle body position, after coating, the needle handle of acupuncture needle is complete to the position of needle point, be placed in rapidly condensation and bathe film forming, this condensation is bathed as the tap water after microfiltration membranes is processed, water temperature is 40 ℃, after 20 minutes, it is bathed and take out from condensation, with distilled water, it is cleaned and make it naturally to dry, after electrode dries naturally, be placed in baking oven, the temperature of controlling baking oven is 70 ℃, after 3 hours by its taking-up, treat that electrode temperature is chilled to normal temperature naturally, put it in refrigerator, keep 1 ℃～5 ℃, save backup.
Embodiment 4
Get the acupuncture needle that diameter of needle body is 0.25mm, by its polishing, oil removing, tap water and deionized water that the hot water that is 80 ℃ by temperature successively, temperature are room temperature are rinsed it well, then in the time of 100 ℃, with baking oven, it are dried, the enameled wire two ends sand papering that is diameter 0.2mm, until the tinsel of 2cm is exposed at its two ends, end soldering by the tinsel of one end and above-mentioned acupuncture needle, by 100gN, the N-dimethyl acetamide is placed in beaker, then the polyethersulfone of 18g is joined to N, in N-dimethyl acetamide organic solvent, controlling solution temperature is 90 ℃, uniform stirring, after the polyethersulfone powder fully dissolves, above-mentioned solution temperature is down to 70 ℃, and the temperature of maintenance solution is 70 ℃, continue to stir 3 hours, generate polyethersulfone film forming film forming liquid used, the position of the needle handle of acupuncture needle and enameled wire welding is immersed in above-mentioned polyethersulfone film forming liquid, enameled wire apart from 3cm place, this welding position also is immersed in polyethersulfone film forming liquid, after 30 seconds by its taking-up, and rapidly the position of soaking polyethersulfone film forming liquid is placed in to condensation bath film forming, this condensation is bathed as the tap water after microfiltration membranes is processed, water temperature is 50 ℃, after 30 minutes by its taking-up, and dry, get the dried cotton ball wet by above-mentioned polyethersulfone film forming immersion, clamp the cotton ball soaked with tweezers, polyethersulfone film forming liquid evenly is coated in to the needle body position of acupuncture needle, also should be applied the part needle handle near needle body position, after coating, the needle handle of acupuncture needle is complete to the position of needle point, be placed in rapidly condensation and bathe film forming, this condensation is bathed as the tap water after microfiltration membranes is processed, water temperature is 50 ℃, after 20 minutes, it is bathed and take out from condensation, with distilled water, it is cleaned and make it naturally to dry, after electrode dries naturally, be placed in baking oven, the temperature of controlling baking oven is 80 ℃, after 3 hours by its taking-up, treat that electrode temperature is chilled to normal temperature naturally, put it in refrigerator, keep 1 ℃～5 ℃, save backup.
Claims (3)
1. the preparation method of a bio-sensing electrode is characterized in that:
(1) get the acupuncture needle that diameter of needle body is 0.18mm～0.25mm, by its polishing, oil removing, tap water and deionized water that the hot water that is 80 ℃ by temperature successively, temperature are room temperature are rinsed it well, then in the time of 100 ℃, with baking oven, it are dried;
(2) the enameled wire two ends sand papering that is diameter 0.2mm, until the tinsel of 1cm～2cm is exposed at its two ends, by the end soldering of the tinsel of one end and above-mentioned acupuncture needle;
(3) by 100 mass parts N, the N-dimethyl acetamide is placed in beaker, then the polyethersulfone of 15～18 mass parts is joined in the DMA organic solvent, controlling solution temperature is 80 ℃～90 ℃, uniform stirring, after the polyethersulfone powder fully dissolves, above-mentioned solution temperature is down to 60 ℃～70 ℃, and the temperature of maintenance solution is 60 ℃～70 ℃, continue to stir 3 hours, generate polyethersulfone film forming film forming liquid used;
(4) the position of the needle handle of acupuncture needle and enameled wire welding is immersed in above-mentioned polyethersulfone film forming liquid, after 30 seconds by its taking-up, and rapidly the position of soaking polyethersulfone film forming liquid is placed in to condensation bath film forming, and this condensation is bathed as the tap water after microfiltration membranes is processed, and water temperature is 40 ℃～50 ℃; After 20 minutes, by its taking-up, and dry;
(5) get the dried cotton ball wet by above-mentioned polyethersulfone film forming immersion, clamp the cotton ball soaked with tweezers, polyethersulfone film forming liquid evenly is coated in to the needle body position of acupuncture needle;
(6) after applying, the needle handle of acupuncture needle to the position of needle point is placed in fully, rapidly to condensation and bathes film forming, after 20 minutes, it is bathed and take out from condensation, with distilled water, it is cleaned and makes it naturally to dry;
(7) after electrode dries naturally, be placed in baking oven, the temperature of controlling baking oven is 60 ℃～80 ℃, after 3 hours, by its taking-up, treats that electrode temperature is chilled to normal temperature naturally, puts it in refrigerator, keeps 1 ℃～5 ℃, saves backup.
2. the preparation method of a kind of bio-sensing electrode according to claim 1, is characterized in that: the enameled wire apart from 3cm place, this welding position is immersed in polyethersulfone film forming liquid.
3. the preparation method of a kind of bio-sensing electrode according to claim 1 and 2, is characterized in that: polyethersulfone film forming liquid evenly is coated on the part needle handle near needle body position.
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| CN201310322310.0A CN103439386B (en) | 2013-07-29 | 2013-07-29 | Preparation method of biological sensing electrode |
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| CN201310322310.0A CN103439386B (en) | 2013-07-29 | 2013-07-29 | Preparation method of biological sensing electrode |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6965791B1 (en) * | 2003-03-26 | 2005-11-15 | Sorenson Medical, Inc. | Implantable biosensor system, apparatus and method |
| CN101975807A (en) * | 2010-09-09 | 2011-02-16 | 天津大学 | Three-dimensional Pt-Pb nano floricome type enzyme-free glucose sensor electrode as well as preparation and application thereof |
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2013
- 2013-07-29 CN CN201310322310.0A patent/CN103439386B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6965791B1 (en) * | 2003-03-26 | 2005-11-15 | Sorenson Medical, Inc. | Implantable biosensor system, apparatus and method |
| CN101975807A (en) * | 2010-09-09 | 2011-02-16 | 天津大学 | Three-dimensional Pt-Pb nano floricome type enzyme-free glucose sensor electrode as well as preparation and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 王倩等: "新型聚醚砜超滤膜的制备与表征", 《膜科学与技术》 * |
| 王章金等: "钙离子传感针的研制", 《传感器技术》 * |
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Granted publication date: 20150408 Termination date: 20170729 |