CN103859589A - Preparation method of squalene and beta-carotene composition liquid proliposomes and method for reducing free radical and harm by adopting proliposomes - Google Patents
Preparation method of squalene and beta-carotene composition liquid proliposomes and method for reducing free radical and harm by adopting proliposomes Download PDFInfo
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Abstract
The invention discloses a preparation method of squalene and beta-carotene composition liquid proliposomes and a method for reducing free radical and harm by adopting proliposomes. The liquid proliposomes is prepared through 5 to 10 parts of phospholipid, 5 to 10 parts of stabilizer, 8 to 15 parts of surface active agent, 20 to 40 parts of squalene and beta-carotene composition and the balance of water. The liquid proliposomes is placed into a hollow cylinder body with two ends being respectively provided with a waterproof and dustproof ventilate membrane, the hollow cylinder body is arranged in a cigarette filter tip of cellulose acetate fibers in the molding process of the cigarette filter tip to obtain a cigarette filter tip capable of reducing the harm of the cigarette, when a consumer smokes the cigarette, the smoke passes through the built-in liquid proliposomes, since the surface area of the liquid proliposomes is large, antioxidant is adequately contacted with mainstream smoke, so that the gas-phase free radical in the mainstream smoke can be effectively eliminated, and powerful technical support can be provided for solving the smoking and health problem.
Description
Technical field
The present invention relates to technical field of chemistry, more specifically, relate to a kind of liquid precursor liposome containing squalene and beta carotene composition and preparation method thereof and fall the application aspect base harm reduction at shredded tobacco for water pipes type filter tip.
Background technology
The harm that thousands of kinds of compounds that smoking produces and the radical pair human body of high concentration cause causes people's generally attention.And the effect of radical pair health endangers the most by force maximum, it can cause tuberculosis, heart disease and cancer, and these radical pairs smoker produces serious injury.Smoking produces the free radical of high concentration, comprises the phase Free Radicals that the life-span is relatively grown, and generation is reacted in quinones and half quinones, multiring aromatic hydrocarbon free radical and they active oxygen radical with oxygen is as superoxide anion (O
2 -), hydrogen peroxide (H
2o
2), hydroxy radical (OH), singlet oxygen (
1o
2) and the gaseous phase free radical relatively short with life period such as lipid peroxidation free radical (ROO), alkoxy free group (RO), alkyl diradical (R), nitrogen oxide free radical etc.
At present in to flue gas aspect radicals scavenging research and few, mainly reduce the injury of radical pair human body flue gas from additional natural, vitamin, amino acid, protein etc. and biology enzyme free radical scavenger and trace element, natural mineral matter.Further, there is the custom of water suction cigarette China some areas, its be cigarette after burning by the water in water pipe after again in suction nozzle, it not only can remove water-soluble harmful chemical, can also remove part gaseous phase free radical, has alleviated the injury of smoking to human body.And can alleviate because smoking causes the probability that human body is got angry.The utility model patent that Luo Fuzhong (2013) is CN 203182005 U at publication number discloses a kind of shredded tobacco for water pipes type filter tip, in water, add surfactant and/or add the Chinese herbal medicine such as peppermint, Radix Glycyrrhizae to extract composition, its object reaching is significantly to reduce tar, nicotine content, can not get angry, also can not affect the taste of tobacco, simple in structure, with low cost; In the utility application that Jiang Zhimin (2008) is ZL200820107184.1 in the patent No., disclose a kind of dipping snuff filter tip, in dipping snuff filter tip structure, improved design.Jiang Huaguo (1998) discloses a kind of Portable water tobacco pipe in its utility application, by simplifying the structure of water tobacco pipe; In the patent of invention that Huang Hui (2005) is CN200510129846.6 at publication number, disclose a kind of method of water tobacco pipe to decoking cigarette and comprehensive harm reduction of utilizing, studied decoking and modulated the liquid formulation of moulding with comprehensive harm reduction.In above-mentioned shredded tobacco for water pipes type filter tip, all do not relate to suitable antioxidant science is added in shredded tobacco for water pipes type cigarette holder, remove the research of gaseous phase free radical in flue gas.
Squalene is a kind of lipid unsaponifiable matter, for the many unsaturated olefins of one (squalene), to find from the liver oil of shark at first, within 1914, be named as Squalene, its chemical name is 2, 6, 10, 15, 19, 23-hexamethyl-2, 6, 10, 14, 18, 22-tetracosa carbon six alkene, belong to open chain triterpene, claim again Squalene, participate in human body cholesterol biosynthesis and can enhance metabolism, there is superoxide dismutase (SOD) activity in the body of raising, strengthen body immunity, anti-ageing, antifatigue, the different physiological roles such as antitumor, it is a kind of avirulent marine bioactivity material with the effect of preventing and curing diseases.Squalene distributes very wide in plant, but content is not high, and many lower than 5% of unsaponifiable matter in vegetable oil, only minority content is more, and as olive oil contains squalene 150～700mg/100g, in rice bran oil, its content is 332mg/100g.
Beta carotene is that one is extensively present in green and yellow vegetable, and the natural in fruit has good anti-chromosome aberration, protective effect on cancer risk and stronger physiologically active.And important potential aroma component in beta carotene or tobacco, the height of its content has a great impact cigarette quality.In tobacco, contain 18 Carotenoids, carotenoid is the precursor of fragrance matter important in tobacco, in alcoholization process, can be biodegradable into more than 80 kind of aroma component through enzyme effect or air oxidation, and their perfume (or spice), taste tools to cigarette have a great impact.Therefore, improve the content of carotenoid in tobacco, can improve the quality of tobacco, and can improve the security of cigarette.
In order to overcome the shortcoming of common antioxidant, reach the object that can simultaneously remove long-life phase Free Radicals and the relatively short gaseous phase free radical of life period, the applicant is devoted for years in correlative study and obtains Patents.In the application that is ZL201010578167.8 in the patent No., disclose squalene and beta carotene composition and the application in cigarette thereof, squalene and beta carotene synergistic composition have directly been added in pipe tobacco, obtained good effect.But further research is found, squalene and beta carotene are easy to oxidation deterioration in air, composition is unstable in the time of application, actual production inconvenience.
Liposome (liposome) is a kind of artificial membrane.In the time that amphiphatic molecule is scattered in water as phosphatide and sphingolipid, the hydrophobic tail of molecule is tended to flock together, and avoids water, and hydrophilic head is exposed to water, form have bilayer structure vesicle, be called liposome.According to textural classification, liposome is divided into unilamelar liposome, multilamelar liposome and liquid precursor liposome.Liquid precursor liposome is a kind of liposome with slow release effect, is connected and composed by many non-concentric chamber, and particle diameter, at 5～50 μ m, has larger envelope volume than conventional liposome, is particularly useful for sealing of water soluble drug.The application of liposome is mainly aspect medical at present.
These free radical scavengers are processed into liquid precursor liposome, and science inserts the research of removing gaseous phase free radical (falling base) in shredded tobacco for water pipes type cigarette holder and have no at present report, more have no technology of preparing and the application report of the liquid precursor liposome based on squalene and beta carotene synergistic composition.
Summary of the invention
The object of the invention is to overcome the application deficiency of existing liquid precursor liposome technology aspect cigarette, and spiny dogfish and beta carotene synergistic composition are in the technical deficiency of cigarette application aspect, and a kind of liquid precursor liposome containing squalene and beta carotene composition is provided.
Another object of the present invention is to provide the preparation method of described liquid precursor liposome.
An also goal of the invention of the present invention is to provide the application of the described liquid precursor liposome containing squalene and beta carotene composition in shredded tobacco for water pipes type filter tip.
Object of the present invention is achieved by the following technical programs:
A kind of liquid precursor liposome containing squalene and beta carotene composition is provided, and described liquid precursor liposome is to be prepared through multi-emulsion method by each component of following mass fraction:
Phosphatide: 5～10 parts;
Stabilizing agent: 5～10 parts;
Surfactant: 8～15 parts;
Squalene and beta carotene composition: 20～40 parts;
The water of surplus;
Above-mentioned each constituent mass umber sum is 100 parts;
Preferably, the described liquid precursor liposome containing squalene and beta carotene composition, described liquid precursor liposome is to be prepared by each component of following mass fraction:
Phosphatide: 6～8 parts;
Stabilizing agent: 7～9 parts;
Surfactant: 10～12 parts;
Squalene and beta carotene composition: 25～35 parts;
The water of surplus;
Above-mentioned each constituent mass umber sum is 100 parts.
Can adopt multi-emulsion method with the described liquid precursor liposome containing squalene and beta carotene composition of above-mentioned each component preparation.
Described phosphatide is at least one in lecithin, soybean lecithin, hydrogenated soya phosphatide; Adopt multiple phosphatide, the mixed proportion of various phosphatide is not done strict restriction.
The preferred hydroxyl starch of described stabilizing agent, more preferably, described hydroxyl starch is at least one in hydroxymethyl starch, HES or hydroxypropul starch, adopts when multiple, mixed proportion is not done strict restriction.
Described surfactant is glycerine.
In described squalene and beta carotene composition, the mass ratio of squalene and beta carotene is 1: 7～7: 1, and the ratio of more optimizing is: 1: 3～3: 1.
Described squalene can be the extract that extracts from plant, animal or microorganism or purified after sterling, can adopt commercial product.
Described beta carotene can be that the carrotene extract that extracts from plant can be also the sterling after purified, can adopt commercial product.
The present invention provides the preparation method of the described liquid precursor liposome containing squalene and beta carotene composition simultaneously, science compatibility raw material, accurately process conditions, overcome squalene and the beta carotene not high shortcoming of solubility in water, successfully prepare the liquid precursor liposome containing squalene and beta carotene mixture synergistic composition, can realize well and be applied to the filter-tip object of shredded tobacco for water pipes type.Described preparation method comprises the following steps:
S1, phosphatide and squalene and beta carotene composition are dissolved in glycerine, obtain mixed system;
S2, it is fully mixed S1 gained mixed system high speed homogenizing, obtain the mixed system of homogenizing;
S3, stabilizing agent and water are mixed to get to the aqueous solution of stabilizing agent, after adding the aqueous solution of stabilizing agent in the mixed system of S2 gained homogenizing, stir, form stable microparticle suspending liquid and obtain the liquid precursor liposome containing squalene and beta carotene composition.
Preferably, described in S2, the process conditions of high speed homogenizing are: 2000r/min, stirring 30min.
The invention provides the liquid precursor liposome containing squalene and beta carotene composition that said method prepares.
Liquid precursor liposome containing squalene and beta carotene composition of the present invention obtains well application in cigarette, specifically be applied to shredded tobacco for water pipes type filter tip, be that described liquid precursor liposome is packed in the hollow cylinder that two ends are water proof and dust proof ventilated membrane, in cigarette filter tip rod forming process, be built in the cigarette filter of acetate fiber.
Preferably application process is: described liquid precursor liposome is packed in the hollow cylinder that two ends are water proof and dust proof ventilated membrane, every packs 0.5～1 gram of described liquid precursor liposome into, in cigarette filter tip rod forming process, be built in the cigarette filter of acetate fiber, built-in 1 of every cigarette filter, obtain reducing the cigarette filter of cigarette harm, in the time of consumer's smoking, flue gas is by built-in liquid precursor liposome, because liquid precursor surface of liposome is long-pending large, antioxidant fully contacts with main flume, can effectively remove the gaseous phase free radical in main flume, provide strong technical support for solving smoking and healthy problem.
Beneficial effect of the present invention:
The present invention is applied to the liposome especially technology of liquid precursor liposome in cigarette first, and success is in conjunction with squalene and beta carotene synergistic composition, sum up suitable compatibility auxiliary agent and proportion compatibility, prepare the liquid precursor liposome containing squalene and beta carotene synergistic composition simplely, the liposome that envelop rate is prepared higher than conventional method, for the further developing application of liposome provides strong technical support, gained liquid precursor liposome is high degree of dispersion fine droplet, decentralization is high, specific area is large, can directly apply in shredded tobacco for water pipes type filter tip.
The present invention is successfully embedded in synergistic composition in liquid precursor liposome, overcome the shortcoming that squalene and beta carotene are easy to oxidation deactivation in air, overcome squalene and the water-fast defect of beta carotene synergistic composition simultaneously, by to squalene and beta carotene mixture synergistic composition microencapsulation, reach the stability that increases squalene and beta carotene mixture synergistic composition, acquisition can be applied to the liquid precursor liposome of cigarette filter.
The present invention accurately limits material combination and preparation process condition, the liquid precursor liposome gas permeability that processing obtains is strong, surface area is large, be applicable to the application demand of cigarette filter, can directly apply to shredded tobacco for water pipes type filter tip, antioxidant can fully contact with main flume, reaches the object of removing free radical in main flume, provides strong technical support for solving smoking and healthy problem.
The specific embodiment
Further describe the present invention below in conjunction with specific embodiment.The test method using in following embodiment if no special instructions, is conventional method; The material, the reagent etc. that use if no special instructions, are reagent and the material that can obtain from conventional commercial sources.
Embodiment 1
Prepare each component according to following mass fraction: 10 grams, lecithin (g); Squalene (the true Bioisystech Co., Ltd in Shanghai, content is greater than 96.0%, and following embodiment is same.But therefore do not limit the present invention, those skilled in the art can adopt the squalene in other sources) 5 grams (g); Beta carotene (Li Nuo bio tech ltd, Zhengzhou, content is greater than 96.0%, and following embodiment is same.But therefore do not limit the present invention, those skilled in the art can adopt the beta carotene in other sources) 15 grams (g); 8 grams of glycerine (g); 7 grams of hydroxymethyl starches (g), 55 grams, water (g).
Preparation process is:
S1, lecithin and squalene and beta carotene composition are dissolved in glycerine, obtain mixed system;
S2, it is fully mixed S1 gained mixed system high speed homogenizing (2000r/min, stir 30min), obtain the mixed system of homogenizing;
S3, stabilizing agent and water are mixed to get to the aqueous solution of stabilizing agent, after adding the aqueous solution of stabilizing agent in the mixed system of S2 gained homogenizing, stir, form stable microparticle suspending liquid and obtain the liquid precursor liposome containing squalene and beta carotene composition.
Embodiment 2
Preparation process is with embodiment 1.Take 10 grams of soybean lecithins, 10 grams of squalenes, 10 grams of beta carotenes are dissolved in 8 grams of glycerine, high speed homogenizing fully mixes it, add again 9 grams of HESs to be dissolved in the aqueous solution of 53 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
Embodiment 3
Preparation process is with embodiment 1.Take 10 grams of hydrogenated soya phosphatides, 15 grams of squalenes, 5 grams of beta carotenes are dissolved in 8 grams of glycerine, high speed homogenizing fully mixes it, add again 5 grams of hydroxymethyl starches to be dissolved in the aqueous solution of 57 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
Embodiment 4
Preparation process is with embodiment 1.Take 8 grams of hydrogenated soya phosphatides, 5 grams of squalenes, 20 grams of beta carotenes are dissolved in 10 grams of glycerine, high speed homogenizing fully mixes it, add again 8 grams of hydroxypropul starch to be dissolved in the aqueous solution of 49 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
Embodiment 5
Preparation process is with embodiment 1.Take 8 grams, lecithin, 10 grams of squalenes, 15 grams of beta carotenes are dissolved in 10 grams of glycerine, high speed homogenizing fully mixes it, add again 8 grams of HESs to be dissolved in the aqueous solution of 49 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
Embodiment 6
Preparation process is with embodiment 1.Take 8 grams of soybean lecithins, 15 grams of squalenes, 10 grams of beta carotenes are dissolved in 10 grams of glycerine, high speed homogenizing fully mixes it, add again 7 grams of hydroxypropul starch to be dissolved in the aqueous solution of 50 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
Embodiment 7
Preparation process is with embodiment 1.Take 8 grams of hydrogenated soya phosphatides, 20 grams of squalenes, 5 grams of beta carotenes are dissolved in 10 grams of glycerine, high speed homogenizing fully mixes it, add again 8 grams of hydroxymethyl starches to be dissolved in the aqueous solution of 49 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
Embodiment 8
Preparation process is with embodiment 1.Take 5 grams of hydrogenated soya phosphatides, 5 grams of squalenes, 25 grams of beta carotenes are dissolved in 12 grams of glycerine, high speed homogenizing fully mixes it, add again 5 grams of HESs to be dissolved in the aqueous solution of 48 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
Embodiment 9
Preparation process is with embodiment 1.Take 5 grams of soybean lecithins, 10 grams of squalenes, 20 grams of beta carotenes are dissolved in 12 grams of glycerine, high speed homogenizing fully mixes it, add again 5 grams of hydroxypropul starch to be dissolved in the aqueous solution of 48 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
Embodiment 10
Preparation process is with embodiment 1.Take 5 grams of soybean lecithins, 15 grams of squalenes, 15 grams of beta carotenes are dissolved in 12 grams of glycerine, high speed homogenizing fully mixes it, add again 5 grams of hydroxypropul starch to be dissolved in the aqueous solution of 48 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
Embodiment 11
Preparation process is with embodiment 1.Take 5 grams of soybean lecithins, 20 grams of squalenes, 10 grams of beta carotenes are dissolved in 12 grams of glycerine, high speed homogenizing fully mixes it, add again 5 grams of HESs to be dissolved in the aqueous solution of 48 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
Embodiment 12
Preparation process is with embodiment 1.Take 5 grams of soybean lecithins, 25 grams of squalenes, 5 grams of beta carotenes are dissolved in 12 grams of glycerine, high speed homogenizing fully mixes it, add again 5 grams of hydroxymethyl starches to be dissolved in the aqueous solution of 48 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
Embodiment 13
Preparation process is with embodiment 1.Take 8 grams, lecithin, 5 grams of squalenes, 35 grams of beta carotenes are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 10 grams of hydroxymethyl starches to be dissolved in the aqueous solution of 27 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
Embodiment 14
Preparation process is with embodiment 1.Take 8 grams, lecithin, 10 grams of squalenes, 30 grams of beta carotenes are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 10 grams of HESs to be dissolved in the aqueous solution of 27 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
Embodiment 15
Preparation process is with embodiment 1.Take 8 grams, lecithin, 15 grams of squalenes, 25 grams of beta carotenes are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 10 grams of hydroxypropul starch to be dissolved in the aqueous solution of 27 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
Embodiment 16
Preparation process is with embodiment 1.Take 6 grams of hydrogenated soya phosphatides, 20 grams of squalenes, 20 grams of beta carotenes are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 7 grams of hydroxymethyl starches to be dissolved in the aqueous solution of 32 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
Embodiment 17
Preparation process is with embodiment 1.Take 8 grams of hydrogenated soya phosphatides, 25 grams of squalenes, 15 grams of beta carotenes are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 5 grams of HESs to be dissolved in the aqueous solution of 32 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
Embodiment 18
Preparation process is with embodiment 1.Take 8 grams of hydrogenated soya phosphatides, 30 grams of squalenes, 10 grams of beta carotenes are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 10 grams of hydroxypropul starch to be dissolved in the aqueous solution of 27 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
Embodiment 19
Preparation process is with embodiment 1.Take 8 grams, lecithin, 35 grams of squalenes, 5 grams of beta carotenes are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 10 grams of hydroxymethyl starches to be dissolved in the aqueous solution of 27 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.
The liquid precursor liposome testing result that above-described embodiment 1～19 is prepared is as follows: adopt laser scattering method to detect, the liquid precursor liposome particle diameter that embodiment 1～19 prepares is 80～140nm, is evenly distributed, and is normality, and span is little.
Adopt dialysis separation method that squalene free in solution is separated with liposome with beta carotene, respectively mensuration, computational envelope rate.According to (in liposome in squalene and beta carotene composition quality/liposome squalene and beta carotene composition gross mass) × 100% computational envelope rate.The liquid precursor liposome encapsulation that the embodiment of the present invention 1～19 prepares is more than 85%.
Comparative example 1
Preparation process is with embodiment 1.Take 3 grams, lecithin, 35 grams of squalenes, 5 grams of beta carotenes are dissolved in 20 grams of glycerine, high speed homogenizing fully mixes it, add again 15 grams of hydroxymethyl starches to be dissolved in the aqueous solution of 22 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.The liquid precursor liposome particle diameter preparing is 210～350nm, skewness.Envelop rate is 48%.
Comparative example 2
Take 8 grams of cephalins, 15 grams of squalenes, 10 grams of beta carotenes are dissolved in 10 grams of propane diols, high speed homogenizing fully mixes it, add again 8 grams of hydroxypropyl methylcelluloses to be dissolved in the aqueous solution of 49 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and beta carotene composition.The liquid precursor liposome particle diameter preparing is 340～650nm, skewness.Envelop rate is 34%.
Embodiment 20 application experiments
The liquid precursor liposome of above-described embodiment 1～19 is packed in the hollow cylinder that two ends are water proof and dust proof ventilated membrane, and every packs 0.5～1 gram into.In cigarette filter tip rod forming process, be placed in the cigarette filter of acetate fiber, built-in 1 of every cigarette filter, obtains reducing the shredded tobacco for water pipes type filter tip of cigarette gaseous phase free radical harm.Not contain the liquid proliposome of blank of squalene or beta carotene, (taking 48 grams of hydrogenated soya phosphatides is dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 10 grams of HESs to be dissolved in the aqueous solution of 27 grams of water, stir, form stable microparticle suspending liquid, obtain blank liquid precursor liposome.) be contrast.
Radicals scavenging effect detection in cigarette smoke: smoke gaseous phase free radical adopts spin catching agent PBN(2-benzene tert-butylnitrone) the rear detection of trapping.
Adopt conventional gaseous phase free radical detection method: adopt glassware to collect flue gas free radical.Add the carbon tetrachloride solution of certain density free radical scavenger PBN in collector, collector is linked on smoking machine, take suction capacity as 35.0mL, suction time is 2s, and suction is spaced apart the standard aspiration speed smoking cigarette of 60s.After smoking, constant volume is collected flue gas free-atom aqueous solution, then puts into liquid nitrogen freezing.Detecting volume is 200 μ L.
The filter-tip cigarette that adds the natural synergistic composition liquid precursor liposome of different content and different proportionings by detection produces the content of free radical, compared with the control, and the removing effect of calculation composition to gaseous phase free radical in cigarette smoke.Experimental result is in table 1:
Show 1:1 gram/
| Sample | Gaseous phase free radical clearance rate (%) |
| Embodiment 1 | 5.82 |
| Embodiment 2 | 9.59 |
| Embodiment 3 | 6.75 |
| Embodiment 4 | 6.39 |
| Embodiment 5 | 10.63 |
| Embodiment 6 | 11.79 |
| Embodiment 7 | 6.98 |
| Embodiment 8 | 7.39 |
| Embodiment 9 | 13.73 |
| Embodiment 10 | 15.68 |
| Embodiment 11 | 12.64 |
| Embodiment 12 | 8.40 |
| Embodiment 13 | 9.04 |
| Embodiment 14 | 16.83 |
| Embodiment 15 | 18.96 |
| Embodiment 16 | 17.48 |
| Embodiment 17 | 19.53 |
| Embodiment 18 | 20.74 |
| Embodiment 19 | 10.68 |
| Blank | 0.26 |
Show 2:0.5 gram/
| Sample | Gaseous phase free radical clearance rate (%) |
| Embodiment 1 | 4.92 |
| Embodiment 2 | 8.55 |
| Embodiment 3 | 6.35 |
| Embodiment 4 | 6.49 |
| Embodiment 5 | 10.23 |
| Embodiment 6 | 11.59 |
| Embodiment 7 | 7.38 |
| Embodiment 8 | 8.59 |
| Embodiment 9 | 13.63 |
| Embodiment 10 | 15.62 |
| Embodiment 11 | 12.65 |
| Embodiment 12 | 8.55 |
| Embodiment 13 | 9.31 |
| Embodiment 14 | 16.48 |
| Embodiment 15 | 18.75 |
| Embodiment 16 | 17.23 |
| Embodiment 17 | 19.67 |
| Embodiment 18 | 20.45 |
| Embodiment 19 | 10.18 |
| Blank | 0.26 |
Experiment conclusion: from above-described embodiment, cigarette gaseous phase free radical being removed to effect can find out, when in liquid precursor liposome containing the amount of squalene and beta carotene composition during in 20～40% left and right, it freely has good removing effect to gas phase, the proportioning of particularly working as squalene and beta carotene is between 1: 7～7: 1, especially between 1: 3～3: 1 time, composition is removed effect to gaseous phase free radical and is had obvious synergistic effect, and its ability of removing free radical strengthens along with the increase of the amount of antioxidant.Actual according to producing, the present invention is for containing squalene and beta carotene in 30～40% left and right in the liquid precursor liposome of the natural composition of cigarette filter, its removing ability synergistic effect to gaseous phase free radical between 1: 3～3: 1 of the proportioning of squalene and beta carotene is obvious.
Claims (10)
1. containing a liquid precursor liposome for squalene and beta carotene composition, it is characterized in that, described liquid precursor liposome is to be prepared by each component of following mass fraction:
Phosphatide: 5～10 parts;
Stabilizing agent: 5～10 parts;
Surfactant: 8～15 parts;
Squalene and beta carotene composition: 20～40 parts;
The water of surplus;
Above-mentioned each constituent mass umber sum is 100 parts;
Described phosphatide is at least one in lecithin, soybean lecithin, hydrogenated soya phosphatide;
Described stabilizing agent is hydroxyl starch;
Described surfactant is glycerine.
2. according to claim 1 containing the liquid precursor liposome of squalene and beta carotene composition, it is characterized in that, is to be prepared by each component of following mass fraction:
Phosphatide: 6～8 parts;
Stabilizing agent: 7～9 parts;
Surfactant: 10～12 parts;
Squalene and beta carotene composition: 25～35 parts;
The water of surplus;
Above-mentioned each constituent mass umber sum is 100 parts.
3. according to the liquid precursor liposome that contains squalene and beta carotene composition described in claim 1 or 2, it is characterized in that, described hydroxyl starch is at least one in hydroxymethyl starch, HES or hydroxypropul starch.
4. according to the liquid precursor liposome that contains squalene and beta carotene composition described in claim 1 or 2, it is characterized in that, in described squalene and beta carotene composition, the mass ratio of squalene and beta carotene is 1: 7～7: 1.
5. according to claim 4 containing the liquid precursor liposome of squalene and beta carotene composition, it is characterized in that, in described squalene and beta carotene composition, the mass ratio of squalene and beta carotene is 1: 3～3: 1.
6. the preparation method who contains the liquid precursor liposome of squalene and beta carotene composition described in claim 1～5 any one, is characterized in that, comprises the following steps:
S1, phosphatide and squalene and beta carotene composition are dissolved in glycerine, obtain mixed system;
S2, it is fully mixed S1 gained mixed system high speed homogenizing, obtain the mixed system of homogenizing;
S3, stabilizing agent and water are mixed to get to the aqueous solution of stabilizing agent, after adding the aqueous solution of stabilizing agent in the mixed system of S2 gained homogenizing, stir, form stable microparticle suspending liquid and obtain the liquid precursor liposome containing squalene and beta carotene composition.
7. according to claim 6 containing the preparation method of the liquid precursor liposome of squalene and beta carotene composition, it is characterized in that, the process conditions of high speed homogenizing are described in S2: 2000r/min, stirring 30min.
8. the liquid precursor liposome containing squalene and beta carotene composition that described in claim 6, method prepares.
9. described in claim 1～5 or 7 any one, contain the application of the liquid precursor liposome of squalene and beta carotene composition, it is characterized in that, be applied to shredded tobacco for water pipes type filter tip, be that described liquid precursor liposome is packed in the hollow cylinder that two ends are water proof and dust proof ventilated membrane, in cigarette filter tip rod forming process, be built in the cigarette filter of acetate fiber.
10. application according to claim 9, is characterized in that, described in built-in 0.5～1 gram of every cigarette filter containing the liquid precursor liposome of squalene and beta carotene composition, more preferably 1 gram.
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| CN105758954A (en) * | 2016-02-29 | 2016-07-13 | 中国烟草总公司广东省公司 | Detecting method for squalene in cigarette smoke |
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| US20050031847A1 (en) * | 2001-07-13 | 2005-02-10 | Nicholas Martens | Products comprising a sheet and a lipid and aqueous phase |
| US20080255498A1 (en) * | 2005-08-25 | 2008-10-16 | Houle Philip R | Sensitizer Solutions, Systems, and Methods of Use |
| CN102079979A (en) * | 2010-12-08 | 2011-06-01 | 华南农业大学 | Composition of squalene and beta-carotene and application thereof in cigarettes |
| WO2012017199A1 (en) * | 2010-08-03 | 2012-02-09 | Royal Holloway And Bedford New College Et Al | Fatty acid esters of carotenoid glucosides as colouring agents for foodstuffs |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050031847A1 (en) * | 2001-07-13 | 2005-02-10 | Nicholas Martens | Products comprising a sheet and a lipid and aqueous phase |
| US20080255498A1 (en) * | 2005-08-25 | 2008-10-16 | Houle Philip R | Sensitizer Solutions, Systems, and Methods of Use |
| WO2012017199A1 (en) * | 2010-08-03 | 2012-02-09 | Royal Holloway And Bedford New College Et Al | Fatty acid esters of carotenoid glucosides as colouring agents for foodstuffs |
| CN102079979A (en) * | 2010-12-08 | 2011-06-01 | 华南农业大学 | Composition of squalene and beta-carotene and application thereof in cigarettes |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105758954A (en) * | 2016-02-29 | 2016-07-13 | 中国烟草总公司广东省公司 | Detecting method for squalene in cigarette smoke |
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