CN104020300A - Detection device and method and equipment for detecting by using device - Google Patents
Detection device and method and equipment for detecting by using device Download PDFInfo
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- CN104020300A CN104020300A CN201310064607.1A CN201310064607A CN104020300A CN 104020300 A CN104020300 A CN 104020300A CN 201310064607 A CN201310064607 A CN 201310064607A CN 104020300 A CN104020300 A CN 104020300A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
Abstract
The invention discloses a detection device for detecting red blood cell types and a method and equipment for detecting by using the device. The detection device comprises a reaction-filtering function layer made of a porous material, wherein the reaction-filtering function layer is used for coating red blood cell antibodies with known types; the antibodies and an antigen of red blood cells with a specific type in a sample to be detected are reacted to agglutinate the red blood cells with the specific type; the pore diameter of the porous material of the reaction-filtering function layer is greater than the diameter of the single red blood cell and is less than the size of the agglutinated red blood cell cluster with the specific type so as to intercept the agglutinated red blood cell cluster with the specific type and enable red blood cells which are not agglutinated to penetrate through.
Description
Technical field
The present invention relates to analytical equipment and method, relate in particular to device and using method thereof that blood is analyzed.
Background technology
Determine that erythrocytic type is not only significant in blood transfusion, and have using value at aspects such as ethnology, science of heredity, medical jurisprudence, trnasplantion immunity, disease resistances.For example, before blood transfusion, must check patient (receptor) and blood donor's (blood donor) blood group, and will carry out cross match blood test.In clinical medicine, except blood transfusion, trnasplantion immunity, to the inspection of neonatal hemolytic disease, autoimmune hemolytic anemia specific antibody, also all need to know blood group.The biological phenomenon of the conventional red cell agglutination of people is determined erythrocytic type.Traditional mode is to determine by artificial.Medical personnel or other operating personnel mix antibody and blood sample, stir, cultivate (reaction incubation) on microslide, and then observe erythrocytic aggegation result.The defect of this mode is that operating personnel are difficult to distinguish red blood cell aggegation and that there is no aggegation, especially for babe in the wood.The correctness of testing result depends on people's business skill.And, need larger sample size just can make eye-observation arrive the red blood cell color of aggegation.In addition,, because the time of mixing, stirring, cultivation needing is longer, this makes to meet demand quick and treatment capacity large (throughput), particularly seems more outstanding in blood bank and this problem of large hospital.
U.S. patent documents US3992158A discloses a kind of integral type analysis component that can be used for analyzing liquid, and these parts at least have two-layer: diffusion layer (spreading layer) and reactant layer, the fluid between this is two-layer is in contact with one another.The liquid sample that diffusion layer can make to be applied on parts distributes equably at this layer, and reactant layer comprises at least one material that can react with liquid to be analyzed, thereby produces and can detect the variation obtaining on parts.This technology utilizes chemical reaction to carry out tracer liquid composition, because chemical reaction velocity itself is just than comparatively fast, so also there are not the needs that need to accelerate the reaction time.
U.S. patent documents US5759774A discloses another and has used the analytical approach of full automatic microplate (microplate) technology.The blood of aggegation and the blood that there is no an aggegation can present different visual forms through cultivating (reaction incubation) and mechanical rock/centrifugation in microplate has the groove that is arranged in arrays of given shape.The method detects operation standardization, and treatment capacity is large, and therefore blood bank or large hospital tend to adopt this automatic microplate to detect blood group.But this structure of the detecting device complexity, cost is high, and, clean microplate consuming time longer, can reduce detection efficiency, if microplate cleaning is simultaneously not thorough, may produce cross pollution, affect the accuracy of testing result.
Summary of the invention
Based on the above-mentioned problems in the prior art, the object of the invention is to design a kind of method and apparatus that detects the device of red blood cell type and use this device to detect.The porosint that use cost of the present invention is low substitutes above-mentioned microslide, analysis component and microplate, make to detect without a large amount of incubation times and centrifugation, reduce the complexity of pick-up unit, make testing process standardization, the personnel that greatly reduce operate the error causing, and can guarantee the accuracy of testing result.
One embodiment of the present of invention provide a kind of pick-up unit for detection of red blood cell type, this pick-up unit comprises: the reaction-filtering function layer being made of porous materials, it is for applying the known erythrocyte antibody (EA) of type, described antibody can react and make the red cell agglutination of particular type with the erythrocytic antigen of particular type in sample to be tested, the aperture of described reaction-filtering function layer porosint is greater than single erythrocytic diameter and is less than the size of the red blood cell group of the particular type after aggegation, for the red blood cell group of the particular type of throttling aggegation, and UA red blood cell is passed.
Preferably, described device also comprises the absorption layer of being made up of absorbent material, and described absorption layer is arranged on below described reaction-filtering function layer.
Preferably, the known erythrocyte antibody (EA) of described type is the antibody being coated in advance on reaction-filtering function layer.
Preferably, reaction-filtering function layer is one deck, also can be two-layer, comprise responding layer and be arranged on the filtering layer below responding layer, described responding layer is for applying the known antibody of type, the aperture of described responding layer porosint is greater than single erythrocytic diameter, the aperture of described filtering layer porosint is greater than single erythrocytic diameter and is less than the size of the red blood cell group of the particular type after aggegation, described filtering layer is used for the red blood cell group of the particular type of throttling aggegation, and UA red blood cell is passed.
Preferably, between described reaction-filtering function layer and absorption layer, also comprise the separate layer of being made by opaque porosint, so that can not see erythrocytic color absorption layer from the top of described device.
Preferably, the aperture of described responding layer porosint is greater than 10 microns, and the aperture of described filtering layer is between 10-100 micron.Preferably, the aperture of reaction-filtering function layer porosint is between 10-100 micron, and the aperture of described separate layer 3 porosints is greater than 10 microns.
Preferably, described device comprises isolated multiple part, and a part is for one-time detection, and each part comprises multiple surveyed areas, and these surveyed areas are coated with respectively the known antibody A of different types, B, D.Preferably, in multiple surveyed areas of described each part, there is one to be blank.
Another one embodiment of the present invention also discloses a kind of method that uses pick-up unit to detect red blood cell type, the method comprises: by standard red blood cell RBCC or the known erythrocytic antibody of type with comprise erythrocytic sample to be tested and add on described pick-up unit, described pick-up unit comprises reaction-filtering function layer that porosint is made, the aperture of described reaction-filtering function layer porosint is greater than single erythrocytic diameter and is less than the size of the red blood cell group of the particular type after aggegation, for the red blood cell group of the particular type of throttling aggegation, and UA red blood cell is passed, with observe its color from the top of pick-up unit.
Preferably, by standard red blood cell RBCC or the known erythrocytic antibody of type with comprise erythrocytic sample to be tested and add on described pick-up unit after, described method also comprises: on described reaction-filtering function layer, add damping fluid.
Preferably, by standard red blood cell RBCC or the known erythrocytic antibody of type with comprise erythrocytic sample to be tested and add on described pick-up unit after, described method also comprises: the absorption layer that setting is made up of absorbent material described reaction-filtering function layer below.
Another one embodiment of the present invention also discloses a kind of equipment for detection of red blood cell type, this equipment comprises: pick-up unit recited above, the pressure apparatus of water and sample to be tested is provided to described pick-up unit, and the water that described pressure apparatus is provided and sample to be tested add the sample adding device on described pick-up unit to.Preferably, described equipment also comprises the pressure apparatus that damping fluid is provided to described pick-up unit, and adds described damping fluid to sample adding device on described pick-up unit.
Preferably, described checkout equipment also comprises optical sensor, this optical sensor be arranged on described pick-up unit above, for the color of collecting and detecting device.
Preferably, described equipment also comprises: the device that the pick-up unit that comprises multiple parts is rolled, thereby detecting after a sample to be tested, making the untapped part of pick-up unit be rolled to the position of adding water and sample to be tested, to detect next sample to be tested; And the device that sample adding device is moved between the different surveyed areas of a test section.
Embodiments of the invention can be achieved as follows technique effect:
1. shorten the time of agglutinating reaction, increased the treatment capacity of unit interval.Because pick-up unit of the present invention adopts porosint, porosint makes sample to be tested can fully contact with erythrocyte antibody (EA), so compare with the prior art that uses microplate to detect with manual detection, the present invention can shorten red cell antigens in sample to be tested and the time of erythrocyte antibody (EA) generation agglutinating reaction.Meanwhile, without centrifugal or rock and just can make erythrocyte antibody (EA) and corresponding red cell antigens generation agglutinating reaction.
2. the porosint adopting in pick-up unit is cheap, and cost is lower.
3. with the mode ratio of manual detection, the sample to be tested amount needing to the color on pick-up unit due to eye-observation is larger, and the checkout equipment in the embodiment of the present invention can must be less by the size Control of the drop of blood dripping, therefore, use the checkout equipment of the optical sensor with collecting and detecting device color in the embodiment of the present invention to detect the amount of required sample to be tested less.
4. compare with the microplate of Reusability, the every part of pick-up unit in the embodiment of the present invention is all only used once, can not make sample to be tested produce cross pollution.
5. use the equipment of the detection red blood cell type in the embodiment of the present invention can realize the erythrocytic type of automatic detection, and without manual operation.
6. compare with the mode that uses naked-eye observation to have or not the red blood cell of aggegation to roll into a ball, use the pick-up unit in the embodiment of the present invention only need to see on pick-up unit have and there is no redness, this makes to observe more easily, exactly erythrocytic aggegation, thereby judges erythrocytic type.
Brief description of the drawings
Other feature of the present invention, feature, advantage and benefit will become more apparent by the detailed description below in conjunction with accompanying drawing.Wherein:
Fig. 1 shows according to the schematic diagram of the pick-up unit of one embodiment of the invention;
Fig. 2 shows the schematic diagram of the pick-up unit of another one embodiment of the present invention;
Fig. 3 shows the schematic diagram of the pick-up unit that comprises multiple parts;
Fig. 4 illustrates the process of utilizing the detection method of one embodiment of the invention to detect;
Fig. 5 illustrates the process of utilizing the detection method of another one embodiment of the present invention to detect;
Fig. 6 shows the schematic diagram of the checkout equipment of one embodiment of the invention.
Embodiment
Describe each embodiment of the present invention in detail in connection with accompanying drawing below.
Agglutination phenomenon is produced by particle aggregation.In the time there is antibody or other material, multiple particles are connected to one by antibody, forms large cluster of grains.Observing antibody aggegation is the method that is commonly used to identify specific antigen.The present invention is that the phenomenon based on red blood cell meeting aggegation in the time there is antibody is made.The principle that detects red blood cell type is as follows: what red blood cell only contained Staphylococal Protein A is A type, and what only contain B antigen is Type B, and containing A and B antigen is AB type; What do not contain A, B antigen is O type.Situation about therefore existing according to antigen-antibody can be judged erythrocytic type.
Fig. 1 shows according to the schematic diagram of the pick-up unit of one embodiment of the invention 6.This pick-up unit 6 comprises the reaction-filtering function layer 1 being made of porous materials.Here " functional layer " is for representing that this layer is not a Physical layer, can realization response can be called " reaction-filtering function layer " with a Physical layer or multiple Physical layer of filtering these two functions.The present invention does not have particular/special requirement to the composition of porosint, can use common and cheap dacron film etc.Reaction-filtering function layer for example, for applying the known erythrocyte antibody (EA) of type, antibody A, B, RH-D.Can before detecting, for example, in the time manufacturing this multilayer pick-up unit, in advance antibody A, B, RH-D be coated on reaction-filtering function layer, wait the dry rear multilayer pick-up unit of preserving of antibody, during for detection, use.Also can be in the time detecting, scene is coated in antibody A, B, RH-D in the zones of different of reaction-filtering function layer.These antibody can react and make the red cell agglutination of particular type with the erythrocytic antigen of particular type in sample to be tested.Sample to be tested can be blood or comprise erythrocytic blood spin-off.The aperture of reaction-filtering function layer 1 porosint is greater than single erythrocytic diameter and is less than the size of the red blood cell group of the particular type after aggegation.Single erythrocytic diameter is conventionally at 5-10 micron, and the size of the red blood cell group after aggegation is generally more than 100 microns.So the aperture of reaction-filtering function layer 1 porosint is between 10-100 micron.Like this, the red blood cell that reaction-filtering function layer 1 can throttling aggegation, and UA red blood cell is passed.
Reaction-filtering function layer 1 can be one deck, and the red blood cell group of the particular type that the aperture of this layer of porosint can throttling aggegation between 10-100 micron makes not have the red blood cell of aggegation to pass simultaneously.Reaction-filtering function layer 1 can be also two-layer.As shown in Figure 1, in the time being two-layer, ground floor is responding layer 11, and the second layer is the filtering layer 12 being arranged on below responding layer.Described responding layer 11 is for applying the known erythrocyte antibody (EA) of type, and the aperture of making responding layer 11 porosints is greater than single erythrocytic diameter, is greater than 10 microns, thereby makes not have the red blood cell of aggegation can pass responding layer 11.Here, the aperture of responding layer, without the diameter of red blood cell group of particular type that is less than aggegation, that is to say that responding layer 11 can allow the red blood cell group of the particular type of aggegation to be trapped in responding layer.In the time that the aperture of responding layer 11 is less than 100 microns, the red blood cell group of particular type can be by throttling in responding layer 11, and in the time that the aperture of responding layer 11 is greater than 100 microns, red blood cell group can flow on filtering layer 12 through responding layer 11.The aperture of filtering layer 12 porosints is greater than single erythrocytic diameter and is less than the size of the red blood cell group after aggegation, be that the aperture of filtering layer 12 is between 10-100 micron, like this, the red blood cell group of the particular type that filtering layer 12 can throttling aggegation, and make not have the red blood cell of aggegation to pass.Between responding layer 11 and filtering layer 12, can, by glued together, also can force together.
Below reaction-filtering function layer 1, can comprise the absorption layer 2 of being made by absorbent material.The conventional thieving paper in analysis field field just can be used as absorption layer 2, and it can produce downward suction to sample to be tested, thereby provides power for red blood cell moves downward.
Preferably, between reaction-filtering function layer 1 and absorption layer 2, be also provided with the separate layer 3 of being made by opaque porosint.The effect of separate layer 3 is to cover erythrocytic color in absorption layer 2, makes operating personnel can not see erythrocytic color absorption layer 2 from the top of pick-up unit 6.Like this, when only on reaction-filtering function layer 1, throttling has the red blood cell group of particular type of aggegation, operating personnel can observe erythrocytic color from the top of device, thereby can determine according to the type of the antibody applying the erythrocytic type of aggegation.The aperture of separate layer multipurpose material need to be greater than 10 microns, so just can make not have the single red blood cell of aggegation to pass.
As shown in Figure 3, pick-up unit 6 is strip, comprises multiple isolated parts 5.Each part 5 comprises multiple surveyed areas 51,52,53, and each surveyed area is coated with respectively the antibody of known type.For example, each vertical bar shown in Fig. 3 left side is a region, and the pick-up unit 6 shown in right side comprises 3 isolated parts 5, and the surveyed area 51,52,53 of every part can be coated with respectively A, B, RH-D antibody.Can adjust as required the type of the number of surveyed area and the antibody of coating.Preferably, every a part of 5 also comprise a white space 54 that does not apply antibody, and this white space 54 is used to form benchmark color region, and handled easily personnel judge the color in other regions.In the time using the image of optical sensor collecting and detecting device, the existence of benchmark color region also can form strikingly color contrast, the convenient color of determining other regions.Meanwhile, if this white space becomes redness in some detect, prove to have occurred that the situations such as red blood cell have appearred contaminated in these parts, these detect unsuccessfully.
Taking a kind of common comprising, erythrocytic sample to be tested---blood detects the method for red blood cell type in example explanation one embodiment of the invention below.Standard red blood cell RBCC(is oppositely shaped) or the known erythrocytic antibody (forward sizing) of type drip on pick-up unit with blood.Oppositely be fixed to the antibody that checks the unknown in serum with the standard red blood cell RBCC of known blood group; Forward is fixed to the antigen that checks the unknown on red blood cell with the standard serum of known antibodies.While using the method for oppositely sizing, in the time detecting, can first standard red blood cell RBCC be dropped on pick-up unit, also can be first by droplet of blood on pick-up unit, or can first standard red blood cell RBCC and blood be mixed, and then drop on pick-up unit.Similarly, while using the method for forward sizing, can be before detection the known erythrocyte antibody (EA) of first precoating type, also can be in the time detecting field-applied type known erythrocyte antibody (EA), the liquid of can certainly first bleeding in the time detecting drips the known erythrocyte antibody (EA) of type again, or first makes the known erythrocyte antibody (EA) of type and blood mix the potpourri that drips again the two.Pick-up unit comprises reaction-filtering function layer 1 that porosint is made, and the aperture of reaction-filtering function layer 1 porosint is greater than single erythrocytic diameter and is less than the size of the red blood cell group of the particular type after aggegation.Like this, if the erythrocytic antigen of the particular type existing in blood and coating or the known erythrocytic antibody of the type of dripping belong to same or corresponding type, just can there is agglutinating reaction in the two, the red blood cell group of the particular type that reaction-filtering function layer 1 just can throttling aggegation.Operating personnel can observe from the top of pick-up unit 6 reaction-filtering function layer 1 for red, thereby can judge erythrocytic type in school work.Otherwise if the red blood cell of certain type existing in blood and the known erythrocyte antibody (EA) of type coating or that drip do not belong to corresponding type, the two can not aggegation, the red blood cell of this type just can pass reaction-filtering function layer 1.Operating personnel can not be red or be light redness from the color of top observing response-filtering function layer 1 of pick-up unit 6.
Fig. 4 illustrates the method work process that oppositely sizing detects of utilizing in one embodiment of the invention.Fig. 4 (a) for splashing into the state of pick-up unit after standard red blood cell RBCC on pick-up unit, and wherein Ctrl is white space 54, and standard red blood cell is without dripping to Ctrl white space.Now, RBCC-A and RBCC-B are red (form with blacking in figure represents).Fig. 4 (b) is for adding the state after blood to be measured, and blood to be measured need to drip to Ctrl white space.Fig. 4 (c) ~ 4(g) what illustrate is at the state that adds that below reaction-filtering function layer 1 the rear pick-up unit of absorption layer 2 presents.Fig. 4 (h) shows and corresponds respectively to 4(c)-4(g) testing result.Wherein+represent that respective regions becomes redness ,-representing not variable color of respective regions, +/-represents random color.
Preferably, after standard red blood cell RBCC or the known erythrocytic antibody of type and blood are dripped on pick-up unit, said method also comprises: on reaction-filtering function layer 1, add damping fluid.Conventional damping fluid is physiological saline, and it can accelerate the flow velocity of blood, thereby can shorten detection time.
Preferably, by standard red blood cell RBCC or the known erythrocytic antibody of type with comprise erythrocytic sample to be tested and add on pick-up unit after, said method can also comprise: the absorption layer 2 that setting is made up of absorbent material below reaction-filtering function layer 1.Absorption layer 2 is set and can produces downward suction to sample to be tested, thereby provide power for red blood cell moves downward.
Fig. 5 illustrates that the detection method of utilizing in one embodiment of the present of invention carries out the process that forward sizing detects.On the different surveyed areas of the pick-up unit using in this embodiment, be coated with in advance the known different antibodies Anti-A of type, Anti-B, Anti-D.Fig. 5 (a) ~ 5(h) state that while using above-mentioned detection device to detect 8 samples to be tested, pick-up unit presents is shown, what Fig. 5 (i) illustrated is corresponding testing result.
Another one embodiment of the present invention also discloses a kind of equipment for detection of red blood cell type, as shown in Figure 6, this equipment comprises: pick-up unit 6 recited above, provide the pumping installations 8 of blood, this pumping installations of 9(that water can also be provided to pick-up unit 6), add pin or the kapillary etc. 10,11 on pick-up unit 6 to by described pumping installations 8,9 with by the blood providing.Preferably, checkout equipment also comprises the pumping installations that the damping fluid such such as physiological saline is provided to described pick-up unit 6, and adds damping fluid to pin or kapillary etc. on pick-up unit 6.
Checkout equipment also comprises the optical sensor 7 such such as CCD camera, this optical sensor 7 be arranged on pick-up unit 6 above, for give pick-up unit 6 take a picture, the image of collecting and detecting device.Pick-up unit 6 can also comprise for analysis image and produce the image analysis module of analysis result.This image analysis module can realize by software.
Preferably, pick-up unit 6 is the strip that comprises multiple parts 5, and every a part of 5 can detect a blood sample to be measured.Checkout equipment also comprise one make that pick-up unit 6 rolls roller 14, finishing after one-time detection, the untapped part 5 of pick-up unit 6 by roller rolled to the position of adding water and blood to be measured, to detect next blood sample to be measured.Pick-up unit also comprises the mechanical mechanism that pin or kapillary etc. 10,11 moved between multiple surveyed areas 51,52,53,54 of a part 5, and this physical construction can be controlled pin or kapillary etc. 10,11 and on different regions, add blood sample to be measured.The mechanical mechanism that can realize above-mentioned functions is very many, and those skilled in the art just can expect realizing the mechanical mechanism of these functions according to its function, specifically forms so just no longer in detail it is described in detail here.
Below to use the pick-up unit 6 that is coated with in advance antibody as the example simple declaration course of work of above-mentioned checkout equipment once.Mechanical mechanism guide needle from blood to be measured of the mid-absorption of test tube of blood to be measured and by this droplet of blood to be measured for example, on a region of above-mentioned detection device (surveyed area 51).The antibody being coated in responding layer dissolves in blood.If erythrocytic antibody belongs to same type in the antibody on pick-up unit and blood, can there is agglutinating reaction in the two very soon.Will detect droplet of blood on pick-up unit after, another one pin can drip to damping fluid on the above-mentioned zone of pick-up unit.Absorption layer is the blood mobile power that provides downward vertically.If generation aggegation, red blood cell group can be filtered a layer throttling, if there is not aggegation, red blood cell can be washed out by water, leaves very faint redness or do not show redness completely on filtering layer.Afterwards, mechanical mechanism reboot pin by droplet of blood to be measured for example, on the another one region of above-mentioned detection device (surveyed area 52), repeat said process, then the image of the camera of checkout equipment meeting collecting and detecting device, image analysis module meeting automatic analysis image, and definite erythrocytic type, produce examining report.。Detecting after a blood sample to be measured, roller meeting roll detection device, makes the untapped part 5 of pick-up unit 6 move to the position of adding water and blood to be measured, to detect next blood sample to be measured.
By drawings and Examples, the present invention has been carried out to detail display and explanation above, but the invention is not restricted to the embodiment that these have disclosed, other schemes that those skilled in the art therefrom derive are also within protection scope of the present invention.Therefore, protection scope of the present invention should be defined by appending claims.
Claims (17)
1. for detection of a pick-up unit for red blood cell type, it is characterized in that, this pick-up unit comprises:
Reaction-filtering function the layer (1) being made of porous materials, it is for applying the known erythrocyte antibody (EA) of type, described antibody can react and make the red cell agglutination of described particular type with the erythrocytic antigen of particular type in sample to be tested, the aperture of described reaction-filtering function layer (1) porosint is greater than single erythrocytic diameter and is less than the size of the red blood cell group of the particular type after aggegation, for the red blood cell group of the particular type of throttling aggegation, and UA red blood cell is passed.
2. device according to claim 1, wherein said device also comprises the absorption layer (2) of being made up of absorbent material, described absorption layer (2) is arranged on below described reaction-filtering function layer (1).
3. device according to claim 1, the known erythrocyte antibody (EA) of wherein said type is the antibody being coated in advance on reaction-filtering function layer (1).
4. device as claimed in claim 1, wherein reaction-filtering function layer (1) is one deck.
5. device as claimed in claim 1, wherein reaction-filtering function layer (1) is two-layer, comprise responding layer (11) and be arranged on the filtering layer (12) below responding layer, described responding layer (11) is for applying the known antibody of type, the aperture of described responding layer (11) porosint is greater than single erythrocytic diameter, the aperture of described filtering layer (12) porosint is greater than single erythrocytic diameter and is less than the size of the red blood cell group of the particular type after aggegation, described filtering layer (12) is for the red blood cell group of the particular type of throttling aggegation, and UA red blood cell is passed.
6. device as claimed in claim 2, wherein said device also comprises the separate layer (3) of being made up of opaque porosint between described reaction-filtering function layer (1) and absorption layer (2), so that can not see erythrocytic color absorption layer (2) from the top of described device.
7. device as claimed in claim 5, the aperture of wherein said responding layer (11) porosint is greater than 10 microns, and the aperture of described filtering layer (12) is between 10-100 micron.
8. device as claimed in claim 6, wherein the aperture of reaction-filtering function layer (1) porosint is between 10-100 micron, and the aperture of described separate layer (3) porosint is greater than 10 microns.
9. device as claimed in claim 1, wherein said device comprises isolated multiple part (5), a part (5) is for one-time detection, each part (5) comprises multiple surveyed areas (51,52,53), these surveyed areas are coated with respectively the known antibody of different types (A, B, D).
10. device as claimed in claim 9, has one to be blank in multiple surveyed areas of wherein said each part (5).
11. 1 kinds of methods that use pick-up unit to detect red blood cell type, is characterized in that, the method comprises:
By standard red blood cell RBCC or the known erythrocytic antibody of type with comprise erythrocytic sample to be tested and add on described pick-up unit, described pick-up unit comprises reaction-filtering function layer (1) that porosint is made, the aperture of described reaction-filtering function layer (1) porosint is greater than single erythrocytic diameter and is less than the size of the red blood cell group of the particular type after aggegation, for the red blood cell group of the particular type of throttling aggegation, and UA red blood cell is passed, and
Observe its color from the top of described pick-up unit (6).
12. methods according to claim 11, wherein by standard red blood cell RBCC or the known erythrocytic antibody of type with comprise erythrocytic sample to be tested and add on described pick-up unit after, described method also comprises: at the upper damping fluid that adds of described reaction-filtering function layer (1).
13. methods according to claim 11, wherein by standard red blood cell RBCC or the known erythrocytic antibody of type with comprise erythrocytic sample to be tested and add on described pick-up unit after, described method also comprises: the absorption layer (2) that setting is made up of absorbent material described reaction-filtering function layer (1) below.
14. 1 kinds of equipment for detection of red blood cell type, is characterized in that, this equipment comprises:
Pick-up unit (6) as described in claim 1-10,
The pressure apparatus (8,9) of sample to be tested is provided to described pick-up unit (6),
The sample to be tested that described pressure apparatus (8,9) is provided adds the sample adding device (10,11) on described pick-up unit (6) to.
15. checkout equipments as claimed in claim 14, wherein this checkout equipment also comprises optical sensor (7), this optical sensor (7) be arranged on described pick-up unit (6) above, for the color of collecting and detecting device (6).
16. checkout equipments as claimed in claim 14, wherein said equipment also comprises the pressure apparatus that damping fluid is provided to described pick-up unit (6), and adds described damping fluid to sample adding device on described pick-up unit (6).
17. checkout equipments as claimed in claim 14, wherein said equipment also comprises:
Make the device of pick-up unit (6) motion that comprises multiple parts (5), thereby detecting after a sample to be tested, make the untapped part of pick-up unit (6) (5) move to the position of adding water and sample to be tested, to detect next sample to be tested; With
The mechanism that sample adding device (10,11) is moved between the different surveyed areas of a part (5).
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| CN201310064607.1A CN104020300A (en) | 2013-02-28 | 2013-02-28 | Detection device and method and equipment for detecting by using device |
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| CN201310064607.1A CN104020300A (en) | 2013-02-28 | 2013-02-28 | Detection device and method and equipment for detecting by using device |
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| CN201310064607.1A Pending CN104020300A (en) | 2013-02-28 | 2013-02-28 | Detection device and method and equipment for detecting by using device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107727851A (en) * | 2017-11-15 | 2018-02-23 | 北京舜雷科技有限公司 | The system for detecting HIT antibody that a kind of immune agglutination and colloid gold chromatographic test paper strip are combined |
| CN110780081A (en) * | 2019-11-29 | 2020-02-11 | 安邦（厦门）生物科技有限公司 | Reagent strip for detecting orthostereotyped blood types, preparation method of reagent strip and reagent card |
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Application publication date: 20140903 |