CN104173118A - Device for establishing rectal transplanted tumor in mice - Google Patents
Device for establishing rectal transplanted tumor in mice Download PDFInfo
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- CN104173118A CN104173118A CN201410416546.5A CN201410416546A CN104173118A CN 104173118 A CN104173118 A CN 104173118A CN 201410416546 A CN201410416546 A CN 201410416546A CN 104173118 A CN104173118 A CN 104173118A
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- stationary pipes
- dilating
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Abstract
The invention relates to a device for establishing a rectal transplanted tumor in mice. The device comprises a dilating fixing tube and a microsyringe. The upper portion of the dilating fixing tube is provided with a linear injection hole in the lengthwise direction. When the device is in use, a needle of the microsyringe is inserted into the linear injection hole; the needle is longer than the linear injection hole. A handheld portion having a bent angle with the dilating fixing tube is connected to the tail end of the dilating fixing tube. A bulb is connected to the tail end of the handheld portion. The handheld portion, the dilating fixing tube and a tube head are internally provided with air holes communicated; the bulb is communicated with the tail end of the communicated air holes; a front end opening of the communicated air holes is arranged in the top of the tube head. After the dilating fixing tube is inserted into the rectum, the bulb nipped restores and produces negative pressure so that the rectum is tightly sucked to the joint of the tube head and the dilating fixing tube. A rectal bevel is formed in an area near the joint of the tube head and the dilating fixing tube; the rectum is tightly attached to the front end of the dilating fixing tube; accordingly, the needle of the microsyringe can be easily inserted into the rectum. The device is simple to operate and high in tumor formation rate.
Description
Technical field
The present invention relates to medical machinery, particularly, is a kind of device of setting up mice rectum transplanted tumor.
Background technology
When setting up animal model for tumour, mostly adopt Subcutaneous tumor cell injection at present.No matter be hepatocarcinoma, gastric cancer, cervical cancer, pulmonary carcinoma etc., all by tumor cell inoculation in the subcutaneous or muscle of mice, form body surface or Subcutaneous tumor.Inoculation forms like this tumor and clinical common abiogenous tumor all have larger difference at aspects such as growing environment and course advancements.If want to set up tumor orthotopic transplantation tumor, majority needs to adopt the methods of operation, such as setting up rectal cancer animal model, abdominal cavity need to be opened, tumor cell is put under intestinal wall, experiment is difficult to operation, cause error more, and postoperative care workload is large, and mortality of animals is high, be also difficult to set up a large amount of animal models for experiment.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of device of setting up mice rectum transplanted tumor, and this device has feature simple to operate.
For solving above technical problem, the technical solution used in the present invention is:
A kind of device of setting up mice rectum transplanted tumor, comprise an expansion stationary pipes and microsyringe, expansion stationary pipes front end face bottom is protruded formation tube head along its length, expansion stationary pipes top offers linear injection orifice along its length, during use, the syringe needle of microsyringe inserts injection orifice, needle length is greater than injection orifice length, and expansion stationary pipes front end face, outside port that the inner side port of injection orifice is positioned at tube head top are positioned at the outer face of expanding stationary pipes; Expansion stationary pipes end connect one with the grip part of expanding stationary pipes and have bending angle, grip part end connects air bag, described grip part, expansion stationary pipes and tube head inside offer the pore of connection, pore end communicated air bags, and pore front end exit is opened in tube head end face.
During use, first will expand stationary pipes front end and be coated with lubricant, insert mice anal fixing, syringe will be inserted through injection pin hole, cell suspension will be injected under colon wall, exit syringe needle, and slowly extract and protect a stationary pipes.Expansion stationary pipes plays the fixedly effect of rectum.Before expanding stationary pipes insertion mice anus, clutch air bag, air-out, expansion stationary pipes is inserted after rectum, and air bag recovers, and the negative pressure of generation sucks rectum in tube head and the binding site of expanding stationary pipes.Tube head forms a rectum knuckle with the near zone of the binding site of expansion stationary pipes, and rectum is adjacent to expansion stationary pipes front end face, makes the easily interior rectum of visiting of syringe needle.
As a kind of preferred scheme, the diameter of injection orifice is 0.45-0.5mm, and length is 8-10mm.
As the preferred scheme of another kind, microsyringe syringe needle diameter is 0.35-0.45mm, and needle length is 14-16mm.
Adopting the present invention to set up mice rectal cancer orthotopic transplantation tumor animal model has easy and simple to handle, little to animal operation, the high convenient and practical advantage of tumor formation rate, under the prerequisite of skilled grasp operating technology, success ratio of inoculation can reach 100%, does not need to carry out special care after operation.The model building up has with common clinical example and has higher similarity, more directly perceived to the observational study pathological change of pathological changes part and that shift, is to carry out the animal model that colorectal cancer study on prevention is more suitable.
Accompanying drawing explanation
Fig. 1 is the structural representation that the present invention sets up the device of mice rectum transplanted tumor.
In figure, 1-expands stationary pipes, 2-microsyringe, 3-tube head, 4-injection orifice, 5-grip part, 6-air bag, 7-pore.
The specific embodiment
As shown in Figure 1, a kind of device of setting up mice rectum transplanted tumor that the present invention is claimed, comprise an expansion stationary pipes 1 and microsyringe 2, expansion stationary pipes 1 front end face bottom is protruded along its length and is formed tube head 3, expansion stationary pipes 1 top offers linear injection orifice 4 along its length, during use, the syringe needle of microsyringe inserts injection orifice 4, needle length is greater than injection orifice 4 length, and expansion stationary pipes 1 front end face, outside port that the inner side port of injection orifice 4 is positioned at tube head top are positioned at the outer face of expanding stationary pipes 1; Expansion stationary pipes 1 end connect one with the grip part 5 of expanding stationary pipes and have bending angle, grip part 5 ends connect air bag 6, described grip part 5, expansion stationary pipes 1 and tube head 3 inside offer the pore 7 of connection, pore 7 end communicated air bags 6, pore 7 front end exit are opened in tube head 3 end faces.
The diameter of injection orifice 4 is 0.45-0.5mm, and length is 8-10mm.The diameter of expansion stationary pipes is 3-4mm, and the length of tube head is 3-4mm.
Microsyringe syringe needle diameter is 0.35-0.45mm, and needle length is 14-16mm, and requirement can be controlled injection volume between 20-50 microlitre, and also available common disposable sterilized injector replaces.The length requirement of syringe needle is very accurate, and the length that its Shen goes out stationary pipes is 4-6mm.
Below the foundation that adopts the human colon carcinoma SW480 mice orthotopic transplantation tumor animal model that the device of setting up mice rectum transplanted tumor of the present invention carries out.
Laboratory animal, SPF level Balb/c nude mice, male and female half and half, purchased from Beijing dimension tonneau China laboratory animal company limited,
Cell strain, human colon cancer cell strain SW480, HCT-116 is given by Australian Newcastle professor Zhang Xudong of university,
Liquid paraffin, Beijing Zhong Lian chemical reagent factory; PBS, voluntarily preparation.
Rectum inoculator, according to the present invention's self-control, structure and specification are as mentioned above.
1ml syringe, Jiangxi Hongda Medical Equipment Group Corp., Ltd., needle gauge, 0.45 * 16.0mm.
Test method: SW-480 cell strain after 48 hours, digests collecting cell with the RBMI1640 culture medium culturing containing 10% hyclone, and PBS washes 1 time, adjusts cell density to 2.5 * 10
7/ ml, stand-by.
Operational approach a: people fixedly mice makes it in dorsal position, and another people holds rectum inoculated tube, clutches air bag, dips in a little liquid paraffin at expansion stationary pipes tube head, from anus, slowly inserts, and the inserting paragraph anus that all submerges, loosens air bag.Syringe extracts cell suspension, and syringe needle is all inserted to injection orifice, slowly injects cell suspension 40 microlitres, and rotary syringe 90 degree, slowly extract syringe needle gently.
The conventional feeding and management of animal after inoculation, the movable and situation of searching for food of observing animal every day, if find that animal has death, dissects in time, and whether observed and recorded rectum place has tumor growth.Be 30 days observing time, and the observation period finishes, and animal is put to death in cervical vertebra dislocation, and anatomic observation becomes tumor situation and lymphatic metastasis situation, tumor production rate and the rate of lymph-node metastasis of statistics mice.
As a result, SW480 cell, Mel-CV cell, HCT-116 cell are respectively inoculated 20 of animals, all become tumor, and tumor formation rate is 100%, and various tumors all have lymph metastasis phenomenon after becoming tumor, but lymph metastasis rate is different.The results are shown in following table.
Various tumor cell tumor formation rate statistical tables
| Cell strain | Number of animals | Inoculum concentration (microlitre) | Inoculating cell amount | Become tumor number | Tumor formation rate % | Lymph metastasis rate |
| SW-480 | 10 | 40 | 1×10 6 | 10 | 100 | 60 |
| HCT-116 | 10 | 40 | 1×10 6 | 10 | 100 | 40 |
Claims (3)
1. a device of setting up mice rectum transplanted tumor, it is characterized in that: comprise an expansion stationary pipes (1) and microsyringe (2), expansion stationary pipes (1) front end face bottom is protruded along its length and is formed tube head (3), expansion stationary pipes (1) top offers linear injection orifice (4) along its length, during use, the syringe needle of microsyringe inserts injection orifice (4), needle length is greater than injection orifice (4) length, the inner side port of injection orifice (4) is positioned at expansion stationary pipes (1) front end face of tube head top, outside port is positioned at the outer face of expansion stationary pipes (1), expansion stationary pipes (1) end connect one with the grip part (5) of expanding stationary pipes and have bending angle, grip part (5) end connects air bag (6), described grip part (5), expansion stationary pipes (1) and tube head (3) inside offer the pore (7) of connection, pore (7) end communicated air bags (6), pore (7) front end exit is opened in tube head (3) end face.
2. the device of setting up mice rectum transplanted tumor according to claim 1, is characterized in that: the diameter of injection orifice (4) is 0.45-0.5mm, and length is 8-10mm.
3. the device of setting up mice rectum transplanted tumor according to claim 1, is characterized in that: microsyringe syringe needle diameter is 0.35-0.45mm, and needle length is 14-16mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410416546.5A CN104173118A (en) | 2014-08-22 | 2014-08-22 | Device for establishing rectal transplanted tumor in mice |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410416546.5A CN104173118A (en) | 2014-08-22 | 2014-08-22 | Device for establishing rectal transplanted tumor in mice |
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| CN104173118A true CN104173118A (en) | 2014-12-03 |
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| CN201410416546.5A Pending CN104173118A (en) | 2014-08-22 | 2014-08-22 | Device for establishing rectal transplanted tumor in mice |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105582600A (en) * | 2015-12-16 | 2016-05-18 | 中国计量学院 | Insect micro-injection method based on manual microsyringe |
| CN117016487A (en) * | 2023-08-02 | 2023-11-10 | 复旦大学附属中山医院 | Construction method of in-situ transplantation tumor model of colorectal cancer of mice |
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| US5370675A (en) * | 1992-08-12 | 1994-12-06 | Vidamed, Inc. | Medical probe device and method |
| WO2001012102A1 (en) * | 1999-08-13 | 2001-02-22 | Enteric Medical Technologies, Inc. | Apparatus for forming implants in gastrointestinal tract and kit for use therewith |
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| CN117016487A (en) * | 2023-08-02 | 2023-11-10 | 复旦大学附属中山医院 | Construction method of in-situ transplantation tumor model of colorectal cancer of mice |
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Application publication date: 20141203 |
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