CN104521527A - Rapid propagation method for hyacinths - Google Patents

Rapid propagation method for hyacinths Download PDF

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Publication number
CN104521527A
CN104521527A CN201510004362.2A CN201510004362A CN104521527A CN 104521527 A CN104521527 A CN 104521527A CN 201510004362 A CN201510004362 A CN 201510004362A CN 104521527 A CN104521527 A CN 104521527A
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China
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ball
seed
balls
mother bulb
mother
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CN104521527B (en
Inventor
贾文杰
王祥宁
崔光芬
吴丽芳
蒋亚莲
段青
马璐琳
杜文文
王继华
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Flower Research Institute of YAAS
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Flower Research Institute of YAAS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

Abstract

The invention provides a rapid propagation method for hyacinths. The method is characterized by comprising the steps that mother balls with developed and robust seed-base root systems are selected out, with the seed-base as a plane, the mother balls are rotatably cut from the inner side of the seed-base by inclining the seed-base by 30 degrees, sulphur powder with the sulphur mass content being 1 % is evenly smeared on cuts of the mother balls, and the cuts are made upward to be aired by 2-3 hours under the sun at the high noon; the mother balls are propagated in a dark culture mode in a well-ventilated culture room with the temperature being 25 DEG C and the humidity being lower than 50 % until small seed balls grow on the mother balls, the small seed balls are stripped off, buried in a matrix and stored for 80-90 days under 25 DEG C for summer dormancy, the small seed balls are taken out, planted in sandy soil good in drainage and managed according to conventions, and seed balls are dug out until June to July of the second year; the seed balls are stored for 80-90 days under 25 DEG C, then planted and dug out until June to July of the third year, and therefore flowered hyacinth seed balls are obtained. The propagation efficiency of the hyacinths is high, and 8-10 small seed balls with the uniform size can be averagely propagated from each mother ball.

Description

A kind of hyacinthine method for quickly breeding
Technical field
The present invention relates to a kind of propagation method, particularly a kind of hyacinthine method for quickly breeding, belongs to technical field of plant culture.
Background technology
Hyacinth is draft napiform root class plant for many years, and bulb is avette, has film quality crust, and epithelium color becomes positive correlation with pattern.When not blooming, shape is as garlic, originates in Mediterranean and Asia Minor one band, is a kind the most fragrant in the plant that can bloom at present.In recent years, along with the raising of China's expanding economy and living standards of the people, hyacinth has become high-grade pot flowers important in China flowers market, and is widely used in courtyard greening, home decoration, flower etc. in red-letter day field.Along with the continuous increase of hyacinth demand, conventional method for tissue culture because efficiency is lower, the more high reason of complex procedures, material and cost of labor, and cannot meet the Production requirement of extensive hyacinth kind ball; Scale cuttage breeding method conventional in addition, although workload is relatively less, reproduction coefficient is also high, easily causes the problem of planting the ball underproduction in a large number by damage by disease and insect infects, brings unnecessary loss to enterprise.Therefore be necessary to be improved prior art.
Summary of the invention
In order to solve, existing hyacinth propagation technique efficiency is lower, propagating materials and cost of labor higher; production routine relative complex; be difficult to large-scale production; the hyacinth method for quickly breeding that Technique on Cuttage Propagation easily by problems such as damage by disease and insect infection, the invention provides a kind of time and labour saving, the rate of increase is high, seed ball growth quality is good, diseases and insect pests resistance is strong, can preserve mother bulb merit, meet the large-scale production needs of high quality seedling.
The present invention is to provide so a kind of hyacinthine method for quickly breeding, it is characterized in that comprising the following steps:
A. basal disc well developed root system, healthy and strong mother bulb is selected annual March ~ April, take basal disc as plane, tilt 30 degree from circumgyration incision mother bulb inside basal disc, depth of cut is 1.4 ~ 1.6cm, diameter is 4.0 ~ 6.0cm, and is the alcohol wipe knife face of 95% by mass concentration after cutting at every turn;
B. by sulphur mass content be the Cosan uniform application of 1% on the mother bulb otch of steps A, and make otch upwards, under solar noon sun air-dry 2 ~ 3 hours;
C. the mother bulb of step B is divided in 25 DEG C, relative moisture lower than 50%, in breezy culturing room, continuous light culture breeding 70 ~ 75 days, until mother bulb grows seed ball; Period observes mother bulb growing state in good time, removes the mother bulb that rots in time;
D. from strip seeds ball the mother bulb of step C, obtain mitogenetic seed ball, it is in the matrix of 35 ~ 40% that this mitogenetic seed bag is embedded in biodiversity content, and with gauze bag parcel, stores and carry out aestivation in 80 ~ 90 days at 25 DEG C; Be the carbendazim antibacterial agent of 1% containing mass ratio in described matrix;
E. 10 ~ November then, by the seed ball of step D through aestivation process, be planted in well-drained sand, planting environment requires that illumination, ventilation condition are good, conveniently manage, until Second Year digs out kind of a ball June ~ July at the transplanting of letter kind ball, implantation methods;
F. kind ball step e dug out was stored after 80 ~ 90 days in 25 DEG C of environment, 10 ~ November the kind ball stored is planted in well-drained sand in Second Year, planting environment requires that illumination, ventilation condition are good, conveniently hyacinth kind ball transplanting, implantation methods manage, until in June, the 3rd ~ July digs out kind of a ball, namely obtain hyacinth kind ball of blooming.
The present invention has following advantages and effect:
1. breed hyacinth seed ball with the present invention, can whole year production be realized in Nostoc commune Vanch indoor, both save land resources, turn improved economic benefit.
2. reproductive efficiency is high, cultivates and compares with cottage propagation, substantially increase hyacinthine reproductive efficiency with conventional organization, and average each mother bulb can breed 8 ~ 10, seed ball of uniform size, and reproductive efficiency improves greatly.
3. save a large amount of manpower and materials, special patterning method only needs manually to cut hyacinth mother bulb once, just can breed 8 ~ 10, hyacinth seed ball, and method is easy, and use Conventional solid medium culture, because callus is grown thickly, transfer more difficult, and number of times is various, usually seed ball bottle outlet is linked into from explant, need human relay working altogether 4 ~ 5 times, each switching need reconfigure solid culture medium, thus wastes a large amount of human and material resources.
4. solve the seedling parental source complexity that conventional breeding of method is produced, a difficult problem for seed ball quality instability, pass through the present invention, seed ball proterties is stablized, seed ball source is single, be easy to standardization, batch production operation, effectively improve seed ball quality, can be the excellent hyacinth kind ball that market provides unified standard.
5. seed ball field cultivation survival rate is higher.Because this method seed ball flourish environment is closer to natural environment, do not need to grow in the blake bottle of high humility, plant ball transplanting survival rate close to 100%.
6. the present invention is in the selection of mother bulb, and fancy points is good, disease-resistant, resistance to insects is stronger hyacinth kind ball can be selected as breeding mother bulb.The kind by method for quickly breeding of the present invention, fancy points is good, disease-resistant, resistance to insects is strong is fixed rapidly and keeps getting off, and effectively shortens breeding cycle.
Embodiment
Below in conjunction with embodiment, the present invention is described further.
Embodiment 1
A. selecting basal disc well developed root system, healthy and strong mother bulb annual March, with lunar shape knife, take basal disc as plane, oblique 30 degree from circumgyration incision mother bulb inside basal disc, degree of depth 1.5cm, diameter 5.0cm; Cut rear use 95% alcohol wipe knife face at every turn;
B. by sulphur mass content be the Cosan uniform application of 1% on the mother bulb otch of steps A, afterwards by otch upwards, under solar noon sun air-dry 3 hours;
C. the mother bulb of step B is divided in 25 DEG C, relative moisture lower than 50%, in the good culturing room of ventilated environment, continuous light culture breeds 72 days, until mother bulb grows seed ball; Period observes mother bulb growing state in good time, removes the mother bulb that rots in time;
D. from strip seeds ball the mother bulb of step C, obtain mitogenetic seed ball, it is in the matrix of 37% that this mitogenetic seed bag is embedded in biodiversity content, and with gauze bag parcel, stores and carry out aestivation in 85 days at 25 DEG C; Be the carbendazim antibacterial agent of 1% containing mass ratio in described matrix;
E. in October the seed ball through step D aestivation process being planted in well-drained sand then, planting environment requires that illumination, ventilation condition are good, conveniently hyacinth kind ball transplanting, implantation methods manage, until the June of Second Year digs out kind of a ball;
F. kind ball step e dug out was placed in 25 DEG C of environment storages after 85 days, seed ball is planted in well-drained sand, planting environment requires that illumination, ventilation condition are good, conveniently hyacinth kind ball transplanting, implantation methods manage, until dig out kind of a ball June of the 3rd year, obtain hyacinth kind ball of blooming.
Average each mother bulb can breed 8, seed ball of uniform size, and reproductive efficiency improves greatly, and keeps the feature that mother bulb is healthy and strong, good and disease-resistant, the pest-resistant performance of fancy points is strong, survival rate 99% after transplanting.
Embodiment 2
Hyacinthine method for quickly breeding, is characterized in that comprising the following steps:
A. selecting basal disc well developed root system, healthy and strong mother bulb annual April, take basal disc as plane, and tilt 30 degree from circumgyration incision mother bulb inside basal disc, depth of cut is 1.4cm, and diameter is 6.0cm, and is the alcohol wipe knife face of 95% by mass concentration after cutting at every turn;
B. by sulphur mass content be the Cosan uniform application of 1% on the mother bulb otch of steps A, and make otch upwards, under solar noon sun air-dry 2 hours;
C. the mother bulb of step B is divided in 25 DEG C, relative moisture lower than 50%, in breezy culturing room, continuous light culture breeds 70 days, until mother bulb grows seed ball; Period observes mother bulb growing state in good time, removes the mother bulb that rots in time;
D. from strip seeds ball the mother bulb of step C, obtain mitogenetic seed ball, it is in the matrix of 35% that this mitogenetic seed bag is embedded in biodiversity content, and with gauze bag parcel, stores and carry out aestivation in 80 days at 25 DEG C; Be the carbendazim antibacterial agent of 1% containing mass ratio in described matrix;
E. November then, by the seed ball of step D through aestivation process, be planted in well-drained sand, planting environment requires that illumination, ventilation condition are good, conveniently manage, until Second Year digs out kind of a ball July at the transplanting of letter kind ball, implantation methods;
F. kind ball step e dug out was stored after 80 days in 25 DEG C of environment, November the kind ball stored is planted in well-drained sand in Second Year, planting environment requires that illumination, ventilation condition are good, conveniently hyacinth kind ball transplanting, implantation methods manage, until in June, the 3rd digs out kind of a ball, namely obtain hyacinth kind ball of blooming.
Average each mother bulb can breed 10, seed ball of uniform size, and reproductive efficiency improves greatly, and keeps the feature that mother bulb is healthy and strong, good and disease-resistant, the pest-resistant performance of fancy points is strong, survival rate 99.5% after transplanting.
Embodiment 3
Hyacinthine method for quickly breeding, is characterized in that comprising the following steps:
A. selecting basal disc well developed root system, healthy and strong mother bulb annual March, take basal disc as plane, and tilt 30 degree from circumgyration incision mother bulb inside basal disc, depth of cut is 1.6cm, and diameter is 6.0cm, and is the alcohol wipe knife face of 95% by mass concentration after cutting at every turn;
B. by sulphur mass content be the Cosan uniform application of 1% on the mother bulb otch of steps A, and make otch upwards, under solar noon sun air-dry 3 hours;
C. the mother bulb of step B is divided in 25 DEG C, relative moisture lower than 50%, in breezy culturing room, continuous light culture breeds 75 days, until mother bulb grows seed ball; Period observes mother bulb growing state in good time, removes the mother bulb that rots in time;
D. from strip seeds ball the mother bulb of step C, obtain mitogenetic seed ball, it is in the matrix of 40% that this mitogenetic seed bag is embedded in biodiversity content, and with gauze bag parcel, stores and carry out aestivation in 90 days at 25 DEG C; Be the carbendazim antibacterial agent of 1% containing mass ratio in described matrix;
E. October then, by the seed ball of step D through aestivation process, be planted in well-drained sand, planting environment requires that illumination, ventilation condition are good, conveniently manage, until Second Year digs out kind of a ball July at the transplanting of letter kind ball, implantation methods;
F. kind ball step e dug out was stored after 90 days in 25 DEG C of environment, November the kind ball stored is planted in well-drained sand in Second Year, planting environment requires that illumination, ventilation condition are good, conveniently hyacinth kind ball transplanting, implantation methods manage, until in July, the 3rd digs out kind of a ball, namely obtain hyacinth kind ball of blooming.
Average each mother bulb can breed 9, seed ball of uniform size, and reproductive efficiency improves greatly, and keeps the feature that mother bulb is healthy and strong, good and disease-resistant, the pest-resistant performance of fancy points is strong, survival rate 99.8% after transplanting.

Claims (1)

1. a hyacinthine method for quickly breeding, is characterized in that comprising the following steps:
A. basal disc well developed root system, healthy and strong mother bulb is selected annual March ~ April, take basal disc as plane, tilt 30 degree from circumgyration incision mother bulb inside basal disc, depth of cut is 1.4 ~ 1.6cm, diameter is 4.0 ~ 6.0cm, and is the alcohol wipe knife face of 95% by mass concentration after cutting at every turn;
B. by sulphur mass content be the Cosan uniform application of 1% on the mother bulb otch of steps A, and make otch upwards, under solar noon sun air-dry 2 ~ 3 hours;
C. the mother bulb of step B is divided in 25 DEG C, relative moisture lower than 50%, in breezy culturing room, continuous light culture breeding 70 ~ 75 days, until mother bulb grows seed ball; Period observes mother bulb growing state in good time, removes the mother bulb that rots in time;
D. from strip seeds ball the mother bulb of step C, obtain mitogenetic seed ball, it is in the matrix of 35 ~ 40% that this mitogenetic seed bag is embedded in biodiversity content, and with gauze bag parcel, stores and carry out aestivation in 80 ~ 90 days at 25 DEG C; Be the carbendazim antibacterial agent of 1% containing mass ratio in described matrix;
E. 10 ~ November then, by the seed ball of step D through aestivation process, be planted in well-drained sand, planting environment requires that illumination, ventilation condition are good, conveniently manage, until Second Year digs out kind of a ball June ~ July at the transplanting of letter kind ball, implantation methods;
F. kind ball step e dug out was stored after 80 ~ 90 days in 25 DEG C of environment, 10 ~ November the kind ball stored is planted in well-drained sand in Second Year, planting environment requires that illumination, ventilation condition are good, conveniently hyacinth kind ball transplanting, implantation methods manage, until in June, the 3rd ~ July digs out kind of a ball, namely obtain hyacinth kind ball of blooming.
CN201510004362.2A 2015-01-06 2015-01-06 Rapid propagation method for hyacinths Active CN104521527B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106212009A (en) * 2016-08-01 2016-12-14 上海交通大学 The borehole propagation method of Narcissus Tazetta L. Var
CN107173195A (en) * 2017-06-08 2017-09-19 合肥慧明瀚生态农业科技有限公司 A kind of hyacinthine ciltivating process

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JP2000197423A (en) * 1998-11-06 2000-07-18 Japan Tobacco Inc Culture and proliferation of solanaceous plant
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CN102511391A (en) * 2011-12-07 2012-06-27 上海交通大学 Hyacinthus orientalis L. in-vitro rapid propagation method
CN103168580A (en) * 2011-12-26 2013-06-26 江苏美尚生态景观股份有限公司 Hormone treatment method for hyacinth cutting scales
CN103891515A (en) * 2014-04-15 2014-07-02 李蓁 Planting method for common grape hyacinth flowers

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106212009A (en) * 2016-08-01 2016-12-14 上海交通大学 The borehole propagation method of Narcissus Tazetta L. Var
CN107173195A (en) * 2017-06-08 2017-09-19 合肥慧明瀚生态农业科技有限公司 A kind of hyacinthine ciltivating process

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