CN104749105A - Quantitative detection device and detection method based on near-infrared optical tweezers excited up-conversion luminescence - Google Patents
Quantitative detection device and detection method based on near-infrared optical tweezers excited up-conversion luminescence Download PDFInfo
- Publication number
- CN104749105A CN104749105A CN201510190040.1A CN201510190040A CN104749105A CN 104749105 A CN104749105 A CN 104749105A CN 201510190040 A CN201510190040 A CN 201510190040A CN 104749105 A CN104749105 A CN 104749105A
- Authority
- CN
- China
- Prior art keywords
- conversion luminescence
- microballoon
- determinand
- light
- conversion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention provides a quantitative detection device and detection method based on near-infrared optical tweezers excited up-conversion luminescence. The device comprises a near-infrared semiconductor laser, wherein emitted laser beams are expanded through a telescope lens group after passing through a single-mode optical fiber and an optical fiber collimator; the expanded laser beams are reflected into the pupil of an objective lens through a dichroscope; the reflected laser beams are gathered in a sample pool to form optical tweezers; forward diffused light of microspheres in an optical trap passes through a collecting mirror, is reflected by the dichroscope, and irradiates onto a diffused light detector after being converged by a lens; the up-conversion luminescence of the microspheres in the optical trap irradiate onto an up-conversion luminescence detector through pin holes after passing through an optical filter and the lens. Objects to be detected are captured by using the specificity of the microspheres, and the objects to be detected enriched on the surfaces of the microspheres are marked with an up-conversion luminescence probe. By using the device, real-time quantitative detection can be performed on the components of metal ions, biological molecules, virus particles and the like. The device is simple in structure. The detection method has the advantages of high sensitivity, high selectivity, high speed and the like.
Description
Technical field
The invention belongs to micro-nano operative technique field, be specifically related to a kind of quantitative testing device and the detection method that excite up-conversion luminescence based near infrared light tweezer.
Background technology
Light tweezer and single beam gradient force ligh trap are the potential wells can catching whole Michaelis and Rayleigh scattering scope particle interacting based on scattering force and radiation pressure gradient force and formed.What work the effect of catching to particle is gradient force, is that axial gradient power can overcome scattering force by particle-stabilised the prerequisite be strapped in light field potential well.So usually need to use the microcobjective of bigger numerical aperture to be assembled by laser beam height, thus the enough strong gradient force of generation realizes catching of particle.At biomedical sector, optical tweezer technology often combines with light microscope technique, realizes the observation of single particulate, catches and handle.Light tweezer is when scattering force is less than gradient force to modal the catching of particulate, and particulate is stabilized among solution by gradient force, forms the state suspended, is called 3 D captured.
Up-conversion luminescence nanomaterial is a kind of special rare earth luminescent material, and low-frequency exciting light can be changed into high-frequency utilizing emitted light by two-photon or multi-photon mechanism by it.
Summary of the invention
Technical matters to be solved by this invention is to provide a kind of based near infrared light tweezer quantitative testing device and detection method.
Optical tweezer technology and up-converting phosphor technology combine by the present invention, propose a kind of by adopting near-infrared excitation light to avoid exciting autofluorescence in sample (raising signal-to-background ratio), thus improve quantitative testing device and the detection method of detection sensitivity, for the detection of the metallic ion in all kinds of complex sample, biomolecule and virion etc.
A kind of based near infrared light tweezer quantitative testing device, comprise near infrared semiconductor laser, described near infrared semiconductor laser gives off laser beam after single-mode fiber, optical fiber collimator, expanded by telescopic lenses group, then enter pupil after object lens through dichroic mirror reflects, be gathered in sample cell through object lens and form light tweezer, in ligh trap, the forward scattering light of microballoon is through condenser, again by dichroic mirror reflects, lens are irradiated on light scattering detector after converging;
In ligh trap, the up-conversion luminescence of microballoon is after barrier filter and lens, is irradiated on up-conversion luminescence detecting device by pin hole.
Also comprise the motorized precision translation stage and illumination LED that support sample cell.
Described near infrared semiconductor laser is polytype near infrared semiconductor laser of power adjustable, wavelength 975nm-989nm.
Described near infrared semiconductor laser gives off laser beam through single-mode fiber, and the energy distribution in its exit is Gaussian distribution.
Described light scattering detector is quadrant photodetector (QPD).
Described up-conversion luminescence signal detector can be point type photoelectric detector (as PMT and APD), also can adopt image device (as CCD or CMOS etc.).In the present invention, all photoelectric detectors all have ability light signal being changed into electric signal output.
Described sample cell is the microslide covering cover glass, or the transparent vessel of inorganic macromolecule material, and its volume is micro updating.
Use the quantitative detecting method of said apparatus, comprise step:
Step one, utilizes microballoon specificity to catch determinand, and marks the determinand being enriched in microsphere surface with up-conversion luminescence probe, forms microballoon-determinand-up-conversion luminescence probe complex, the i.e. microballoon of enrichment determinand;
Step 2, is placed in sample cell by the microballoon of enrichment determinand, utilizes object lens focus on the laser beam meeting up-conversion luminescence probe shooting conditions of near infrared semiconductor laser generation and in sample cell, form light tweezer;
Step 3, utilize motorized precision translation stage mobile example pond, catch the microballoon of enrichment determinand, light scattering detector synchronously detects the scattered light from sample cell; When the scattered light intensity detected reaches predetermined threshold value, motorized precision translation stage stops mobile, starts the detection carrying out up-conversion luminescence signal;
Step 4, carries out quantitative test according to the Up-conversion Intensity that up-conversion luminescence detecting device detects to determinand.
To be that metallic ion, biomolecule or virion etc. are multiple affine or chemical coupling mode can be enriched to the composition to be measured of microsphere surface by specific biological to described determinand.
Described microballoon is transparent inorganic microsphere or transparent polymer microsphere, and inorganic microsphere can be microballoon prepared by the materials such as silicon dioxide, and polymer microsphere can be microballoon prepared by the materials such as polystyrene.
Described up-conversion luminescence probe is rear-earth-doped up-conversion luminescence nano particle.
Detection method can adopt multiple method specific enrichment determinand.As by dual anti-immuno-sandwich method, utilize microballoon and the identification of up-conversion luminescence probe specificity and catch determinand, forming microballoon-determinand-up-conversion luminescence probe complex; Then move by the two-dimentional automatic precision in the Electrocontrolled sample pond focusing on near-infrared laser and adopt QPD detecting device Quantitative Monitoring microsphere surface scattered light to catch multiple composite inorganic membranes one by one, utilizing up-conversion luminescence detecting device to carry out up-conversion luminescence detection with the quantitative test realizing determinand to composite inorganic membranes simultaneously.
The light tweezer that apparatus of the present invention adopt catches with up-conversion luminescence platform to be microscope, to adopt two-dimentional motorized precision translation stage to reach the demand of mobile example.Simultaneously, the different up-conversion luminescence probe of detection method marks the different determinands with microballoon enrichment, adopt high sensitivity point type photoelectric detector to detect the intensity of the up-conversion luminescence signal of single microballoon, image device also can be adopted to obtain the up-conversion luminescence image of single microballoon.
Compared with prior art, the present invention has following characteristics and beneficial effect:
1, optical tweezer technology and up-converting phosphor technology combine by apparatus of the present invention, and propose a kind of novel quantitative testing device, this device quantitatively can detect multiple composition to be measured.
2, detection method have highly sensitive, selectivity good, speed is fast, amount of samples is few and without the need to advantages such as pre-service.
3, the 980nm near-infrared laser that apparatus of the present invention adopt can not excite the autofluorescence in living things system, avoid the interference of autofluorescence, significantly improve the signal-to-background ratio of detection, thus raising detection sensitivity, therefore the detection of the Multiple components in complex sample is applicable to, without the need to being separated microballoon to be measured, effectively simplify testing process.
Accompanying drawing explanation
Fig. 1 is apparatus of the present invention light path and structural representation;
Fig. 2 is working curve schematic diagram.
Embodiment
As shown in Figure 1, a kind of based near infrared light tweezer quantitative testing device, comprise near infrared semiconductor laser, described near infrared semiconductor laser gives off laser beam after single-mode fiber, optical fiber collimator, is expanded by telescopic lenses group, pupil after object lens is entered again through dichroic mirror reflects, be gathered in sample cell through object lens and form light tweezer, in ligh trap, the forward scattering light of microballoon is through condenser, then by dichroic mirror reflects, lens are irradiated on light scattering detector after converging;
In ligh trap, the up-conversion luminescence of microballoon is after barrier filter and lens, is irradiated on up-conversion luminescence detecting device by pin hole.
The laser beam that near infrared semiconductor laser of the present invention produces is through collimating mirror and telescopic lenses group (L1, L2) beams converge degree is adjusted, make laser beam be full of the rear pupil of object lens, the laser beam after the adjustment of telescopic lenses group injects dichroic mirror (DM).
Dichroic mirror reflects laser enters the object lens in appropriate value aperture, to focus on bottom sample cell and form ligh trap and light tweezer through object lens.Scattered light signal (wavelength is identical with laser) from microballoon converges as directional light through condenser, then is reflected by DM, converges, fall to being mapped on light scattering detector through lens L3.
High-NA objective is adopted in the present invention.The small container of to be cover glass or material the be inorganic polymer transparent material of the sample cell in the present invention.
Dichroic mirror DM is short pass filter, can through visible ray, reflect near infrared light.Therefore laser beam is reflexed to object lens by dichroic mirror DM and focuses on formation light tweezer; By the near infrared forward-scattering signal of microballoon scattering in sample cell through the convergence of condenser, reflected by a same dichroic mirror DM, focus on to fall to being mapped to light scattering detector through lens L3.Meanwhile, the up-conversion luminescence signal being positioned at visible region returned by microsphere surface, then through DM, after being focused on, is detected by up-conversion luminescence detecting device by pin hole by barrier filter (BF) and focus lamp L4.
This device adopts two-dimentional motorized precision translation stage to realize the fast Acquisition one by one of many microballoons and up-conversion luminescence measures, and catches and detect can carry out simultaneously with up-conversion luminescence.
When there is certain density microballoon-determinand-up-conversion luminescence probe complex microballoon in solution in sample cell, and when trapping stiffness is enough, a certain microballoon in mobile can be caught by ligh trap near ligh trap.In this process, light scattering detector whole process detects the near-infrared light scattering light from microballoon, and when its intensity reaches the threshold value preset, motorized precision translation stage system stops mobile, and up-conversion luminescence detecting device detects the Up-conversion Intensity from microballoon.When after the mensuration completing a microballoon, motorized precision translation stage continues mobile, catches and detects next microballoon, until the up-conversion luminescence completing some microballoons detects.
The present invention carries out quantitative test by the Up-conversion Intensity from microballoon measured to the determinand of the enrichment of microsphere surface, can adopt working curve method, specific as follows:
First, the immune microsphere of certain determinand is prepared.Then, according to concentration gradient preparation at least 6 parts of determinand standard solution (comprising blank solution) of setting, identical microballoon immuno-sandwich method (up-conversion luminescence probe used is also identical) is adopted to analyze this serial determinand standard solution respectively, every part of standard specimen is all measured to the Up-conversion Intensity of at least 50 microballoons, get its mean value, drawing curve, i.e. testing concentration and Up-conversion Intensity curve, this curve is straight line within the specific limits.Finally, measure the Up-conversion Intensity of identical determinand contained by unknown sample by the same way, calculate determinand content according to above working curve.
Fig. 2 is the schematic diagram of working curve standard measure, prepare 7 parts of determinand standard solution (containing blank solution) altogether, the up-conversion luminescence of each standard model all at least replicate determination 50 microballoons, averages, and calculates its standard deviation and often upper mark is out in the drawings by it simultaneously.The horizontal ordinate of this curve is determinand concentration of standard solution, and ordinate is Up-conversion Intensity, if the Up-conversion Intensity of determinand is in the range of linearity of working curve in sample, then can realize the quantitative test to determinand.
Below embodiment 1 ~ 2 pair of detection method is described further.
Embodiment 1
The quantitative test of influenza A virus H9N2
Using polystyrene microsphere as carrier, adopt dual anti-immuno-sandwich method to carry out enrichment to influenza A virus, specifically modify microsphere surface, with UCNPs labeled monoclonal antibody with monoclonal antibody.
First, immune microsphere is prepared.Get the polystyrene microsphere that surface carboxyl groups is modified, adopt chemical coupling method to obtain immune microsphere in the anti-HA monoclonal antibody of its surperficial coupling H9N2 virus, for subsequent use.
H9N2 avian influenza virus is quantitatively detected
Enrichment H9N2 avian influenza virus on immune microsphere: add finite concentration (10 in containing the sample solution of H9N2 avian influenza virus
4-10
6individual/mL) immune microsphere in, and add the monoclonal antibody being marked with UCNPs at 37 DEG C, hatch the microballoon that 1 hours obtains enrichment determinand, the emission wavelength ranges of the UCNPs adopted is 547 ± 25nm.
This device is adopted to measure the up-conversion luminescence signal intensity of 50-100 microballoon, and quantitative with mean value; According to the up-conversion luminescence signal intensity measured, adopt working curve method to obtain the concentration of determinand, thus realize quantitatively detecting.Testing result precision (RSD) can reach 5%, detects to be limited to can detect about 50 virions in the reaction system of 100 μ L, and the detectability of fluorescence quantifying PCR method the sensitiveest in Viral diagnosis present is at present on the same order of magnitude.
Embodiment 2
The quantitative test of lung cancer marker CEA
Using polystyrene microsphere as carrier, adopt aptamers coupling method to carry out enrichment to CEA, specifically modify microsphere surface with the aptamers of CEA, mark aptamers with UCNPs.Article two, aptamers, aptamers 1 sequence is: 3 '-ATACCAGCTTATTCAATT-5 ', aptamers 2 sequence is: 3 '-AGGGGGTGAAGGGATACCC-5 ', article two, the different loci on the corresponding CEA antigen of aptamers difference, aptamers 1 is for the preparation of catching microballoon, and aptamers 2 is for the preparation of upper transition probe.
First, microballoon is caught in preparation.Get the polystyrene microsphere that surface carboxyl groups is modified, adopt chemical coupling method to obtain catching microballoon in the aptamers of its surperficial coupling CEA, for subsequent use.
Then, in containing the sample solution of CEA, finite concentration (10 is added
4-10
6individual/mL) catch microballoon, and add be marked with UCNPs aptamers in incubated at room temperature 1 hours, the emission wavelength of the UCNPs adopted is 547 ± 25nm.
This device is adopted to measure the individual up-conversion luminescence signal intensity being enriched the microballoon of CEA of 50-100, and quantitative with mean value; According to the up-conversion luminescence signal intensity measured, working curve method is adopted quantitatively to detect CEA.Testing result precision (RSD) can reach 5%, and detectability is at the order of magnitude of pg/mL.Improve about two orders of magnitude than conventional clinical testing procedure, there is good application prospect.
Claims (10)
1. one kind excites the quantitative testing device of up-conversion luminescence based near infrared light tweezer, it is characterized in that, comprise near infrared semiconductor laser, described near infrared semiconductor laser gives off laser beam after single-mode fiber, optical fiber collimator, expanded by telescopic lenses group, pupil after object lens is entered again through dichroic mirror reflects, be gathered in sample cell through object lens and form light tweezer, in ligh trap, the forward scattering light of microballoon is through condenser, again by dichroic mirror reflects, lens are irradiated on light scattering detector after converging;
In ligh trap, the up-conversion luminescence of microballoon is after barrier filter and lens, is irradiated on up-conversion luminescence detecting device by pin hole.
2. pick-up unit according to claim 1, is characterized in that, also comprises the motorized precision translation stage and illumination LED that support sample cell.
3. pick-up unit according to claim 1, is characterized in that, described near infrared semiconductor laser power adjustable, wavelength 975nm-989nm.
4. pick-up unit according to claim 1, is characterized in that, described light scattering detector is quadrant photodetector.
5. pick-up unit according to claim 1, is characterized in that, described up-conversion luminescence signal detector is point type photoelectric detector, or CCD or CMOS.
6. use the quantitative detecting method of device described in claim 1, comprise step:
Step one, utilizes microballoon specificity to catch determinand, and marks the determinand being enriched in microsphere surface with up-conversion luminescence probe, forms microballoon-determinand-up-conversion luminescence probe complex, the i.e. microballoon of enrichment determinand;
Step 2, is placed in sample cell by the microballoon of enrichment determinand, utilizes object lens focus on the laser beam meeting up-conversion luminescence probe shooting conditions of near infrared semiconductor laser generation and in sample cell, form light tweezer;
Step 3, utilize motorized precision translation stage mobile example pond, catch the microballoon of enrichment determinand, light scattering detector synchronously detects the scattered light from sample cell; When the scattered light intensity detected reaches predetermined threshold value, motorized precision translation stage stops mobile, starts the detection carrying out up-conversion luminescence signal;
Step 4, carries out quantitative test according to the Up-conversion Intensity that up-conversion luminescence detecting device detects to determinand.
7. quantitative detecting method according to claim 6, is characterized in that, described determinand is metallic ion, biomolecule or virion.
8. quantitative detecting method according to claim 6, is characterized in that, described microballoon is transparent inorganic microsphere or transparent polymer microsphere.
9. quantitative detecting method according to claim 8, described inorganic microsphere is prepared by silicon dioxide, and described polymer microsphere is prepared by polystyrene.
10. quantitative detecting method according to claim 6, is characterized in that, described up-conversion luminescence probe is rear-earth-doped up-conversion luminescence nano particle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510190040.1A CN104749105A (en) | 2015-04-21 | 2015-04-21 | Quantitative detection device and detection method based on near-infrared optical tweezers excited up-conversion luminescence |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510190040.1A CN104749105A (en) | 2015-04-21 | 2015-04-21 | Quantitative detection device and detection method based on near-infrared optical tweezers excited up-conversion luminescence |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104749105A true CN104749105A (en) | 2015-07-01 |
Family
ID=53589117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510190040.1A Pending CN104749105A (en) | 2015-04-21 | 2015-04-21 | Quantitative detection device and detection method based on near-infrared optical tweezers excited up-conversion luminescence |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104749105A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106373706A (en) * | 2016-09-05 | 2017-02-01 | 电子科技大学 | Double-focusing field electromagnetic tweezer implementation method based on time reversal spatial and temporal focusing mechanism |
| CN108375560A (en) * | 2017-10-25 | 2018-08-07 | 南京大学 | A kind of laser co-focusing-two-photon-goes up changing multi state fluoroscopic imaging systems and its application |
| CN108717934A (en) * | 2018-05-23 | 2018-10-30 | 上海华力微电子有限公司 | metal ion monitoring method |
| CN109147983A (en) * | 2018-09-18 | 2019-01-04 | 湖北第二师范学院 | One kind is based on just setting microscopical single beam laser light forceps device |
| CN110082282A (en) * | 2019-04-18 | 2019-08-02 | 天津大学 | The method and apparatus for realizing optical ultra-discrimination imaging based on optical tweezer |
| WO2022082930A1 (en) * | 2020-10-22 | 2022-04-28 | 南京诺源医疗器械有限公司 | Fluorescence microscope system for visible-near-infrared real-time image fusion |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6159686A (en) * | 1992-09-14 | 2000-12-12 | Sri International | Up-converting reporters for biological and other assays |
| US6399397B1 (en) * | 1992-09-14 | 2002-06-04 | Sri International | Up-converting reporters for biological and other assays using laser excitation techniques |
| US6537829B1 (en) * | 1992-09-14 | 2003-03-25 | Sri International | Up-converting reporters for biological and other assays using laser excitation techniques |
| CN102998293A (en) * | 2012-12-20 | 2013-03-27 | 武汉大学 | Multichannel quantitative detection device and detection method of two-photon fluorescence optical tweezers |
| CN103913444A (en) * | 2014-04-25 | 2014-07-09 | 武汉大学 | Single-photon fluorescence excitation multi-channel quantitative determination device and detection method based on blue light optical tweezers |
-
2015
- 2015-04-21 CN CN201510190040.1A patent/CN104749105A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6159686A (en) * | 1992-09-14 | 2000-12-12 | Sri International | Up-converting reporters for biological and other assays |
| US6399397B1 (en) * | 1992-09-14 | 2002-06-04 | Sri International | Up-converting reporters for biological and other assays using laser excitation techniques |
| US6537829B1 (en) * | 1992-09-14 | 2003-03-25 | Sri International | Up-converting reporters for biological and other assays using laser excitation techniques |
| CN102998293A (en) * | 2012-12-20 | 2013-03-27 | 武汉大学 | Multichannel quantitative detection device and detection method of two-photon fluorescence optical tweezers |
| CN103913444A (en) * | 2014-04-25 | 2014-07-09 | 武汉大学 | Single-photon fluorescence excitation multi-channel quantitative determination device and detection method based on blue light optical tweezers |
Non-Patent Citations (1)
| Title |
|---|
| WENXIA YANG,ET.AL: "Highly sensitive detection of protein kinase activity using upconversion luminescent nanoparticles", 《RSC ADVANCES》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106373706A (en) * | 2016-09-05 | 2017-02-01 | 电子科技大学 | Double-focusing field electromagnetic tweezer implementation method based on time reversal spatial and temporal focusing mechanism |
| CN106373706B (en) * | 2016-09-05 | 2018-06-19 | 电子科技大学 | The double focusing field electromagnetism tweezer implementation method of focus mechanism during based on time reversal sky |
| CN108375560A (en) * | 2017-10-25 | 2018-08-07 | 南京大学 | A kind of laser co-focusing-two-photon-goes up changing multi state fluoroscopic imaging systems and its application |
| CN108717934A (en) * | 2018-05-23 | 2018-10-30 | 上海华力微电子有限公司 | metal ion monitoring method |
| CN109147983A (en) * | 2018-09-18 | 2019-01-04 | 湖北第二师范学院 | One kind is based on just setting microscopical single beam laser light forceps device |
| CN110082282A (en) * | 2019-04-18 | 2019-08-02 | 天津大学 | The method and apparatus for realizing optical ultra-discrimination imaging based on optical tweezer |
| WO2022082930A1 (en) * | 2020-10-22 | 2022-04-28 | 南京诺源医疗器械有限公司 | Fluorescence microscope system for visible-near-infrared real-time image fusion |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104749105A (en) | Quantitative detection device and detection method based on near-infrared optical tweezers excited up-conversion luminescence | |
| Oheim et al. | Calibrating evanescent-wave penetration depths for biological TIRF microscopy | |
| CN102998293B (en) | Multichannel quantitative detection device and detection method of two-photon fluorescence optical tweezers | |
| CN108387505B (en) | Multifunctional optical tweezers system and method based on micro-fluidic chip | |
| JP6099108B2 (en) | Apparatus and method for detecting a substance to be detected | |
| JP4923065B2 (en) | Sample collection apparatus and method for detection of fluorescently labeled biological components | |
| JP5802653B2 (en) | Optical analysis apparatus, optical analysis method, and computer program for optical analysis | |
| Welsh et al. | A compendium of single extracellular vesicle flow cytometry | |
| Arpali et al. | High-throughput screening of large volumes of whole blood using structured illumination and fluorescent on-chip imaging | |
| CN103913444A (en) | Single-photon fluorescence excitation multi-channel quantitative determination device and detection method based on blue light optical tweezers | |
| CN105300943B (en) | A kind of microscope integrated optical circuit system for drop fluorescence detection | |
| JP2015531490A (en) | Nanoparticle detection method | |
| US20220326249A1 (en) | Method for Detecting Analyte and System for Detecting Analyte | |
| CN103430009A (en) | Axial light loss sensor system for flow cytometery | |
| CN106198951B (en) | A kind of bio-sensing scaling method, calibration system and disease detecting system | |
| CN100543460C (en) | A kind of high flux real-time minimum multifunctional fluorescent detector | |
| CN106124477B (en) | The detection method of concentration of silver ions and rate is discharged in a kind of nano silver course of dissolution | |
| JP5673211B2 (en) | Optical specimen detector | |
| US20150056649A1 (en) | Method for quantifying cell of interest in blood, and method for evaluating system for quantifying said cell | |
| US20170030833A1 (en) | Surface plasmon enhanced fluorescence measurement device and surface plasmon enhanced fluorescence measurement method | |
| Yorulmaz et al. | Single-particle imaging for biosensor applications | |
| CN110462374B (en) | Method and system for analyzing biological samples having label-free cells for the presence of infectious agents | |
| WO2014188621A1 (en) | Examination method and sensor | |
| WO2013136891A1 (en) | Spectroscopic device, spectroscopic lens and spectroscopic container | |
| JP2003028796A (en) | Height calibration method for sample in sample analyzer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150701 |
|
| RJ01 | Rejection of invention patent application after publication |