CN105259240A - Rapid sample application device for PCR product electrophoresis - Google Patents
Rapid sample application device for PCR product electrophoresis Download PDFInfo
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- CN105259240A CN105259240A CN201510650641.6A CN201510650641A CN105259240A CN 105259240 A CN105259240 A CN 105259240A CN 201510650641 A CN201510650641 A CN 201510650641A CN 105259240 A CN105259240 A CN 105259240A
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Abstract
The invention relates to a rapid sample application device for PCR product electrophoresis. The rapid sample application device comprises a base and a non-water-absorption sample adding paper scrap. Multiple PCR pipe containing grooves are formed in the base. Multiple sample adding paper scrap fixing rods are movably connected to the base. Multiple sample adding grooves are formed in the non-water-absorption sample adding paper scrap. A notch is formed in the corner of the connecting end, nearest to the sample adding paper scrap fixing rods, of the non-water-absorption sample adding paper scrap, and the non-water-absorption sample adding paper scrap is pressed and fixed to the base through the paper scrap fixing rods. The rapid sample application device for PCR product electrophoresis further comprises a connecting rod, and the sample adding paper scrap fixing rods are all connected with the connecting rod. Eight samples can be applied at the same time, sample application procedures are reduced, and working efficiency is improved. Sample fixing parts are arranged, the problem that the samples are easily disordered is effectively solved, and the situation that the samples are contaminated due to the fact that the samples are mixed together is avoided. In addition, the sample adding paper scrap fixing rods can be opened and pressed at the same time, and use is convenient.
Description
Technical field
The present invention relates to a kind of PCR primer electrophoresis Quick-Point sampling device.
Background technology
PCR primer electrophoresis is the gene magnification laboratory common technology detecting the PCR reaction whether fast and low-cost such as success, PCR primer clip size, the operation of point sample and the interpretation of rate electrophoresis efficiency and result before PCR primer electrophoresis.Current PCR primer electrophoresis point sample is without stationary installation, and between each laboratory, operation differs, and main apparatus is single channel pipettor and suction pipette head, PE gloves or preservative film.The step of PCR primer electrophoresis is as follows, first PE gloves or preservative film are layered on smooth desktop, then single channel pipettor electrophoresis is used by 1-5ml sample-loading buffer (loadingbuffer) point on PE gloves or preservative film, each PCR primer point, subsequently again with single channel pipettor get from each PCR pipe 1-5mlPCR product mix with the electrophoresis sample-loading buffer that PE gloves or preservative film are put after together with click and enter electrophoresis apparatus agarose or polyacrylamide gel sample well in.Existing PCR primer electrophoresis point sample efficiency is low, when PCR primer quantity is large, above printing operation takes time and effort, be difficult to meet and use, the PE gloves of existing point sample or preservative film are not easy to fix, and easily wave, therefore superincumbent PCR primer is put not only easily chaotic, if PE gloves or the preservative film appearance of having put PCR primer drop or wave, different PCR primer will mix and cause sample contamination, and all that has been achieved is spoiled.
Summary of the invention
The technical problem to be solved in the present invention is: provide a kind of hyperchannel point sample, improve work efficiency, effectively prevents from sample from mixing causing the PCR primer electrophoresis Quick-Point sampling device of sample confusion, solves above-mentioned prior art Problems existing.
The technical scheme solved the problems of the technologies described above is: a kind of PCR primer electrophoresis Quick-Point sampling device, comprise base and the application of sample scraps of paper that do not absorb water, described base is provided with multiple PCR pipe standing groove, described base is flexibly connected and is provided with N number of application of sample scraps of paper fixed bar, the described application of sample scraps of paper that do not absorb water are provided with multiple application of sample groove, the described application of sample scraps of paper that do not absorb water are provided with breach near a jiao of application of sample scraps of paper fixed bar link, the application of sample scraps of paper that do not absorb water are pressed abd fixed on base by scraps of paper fixed bar, and the value of described N is 3 ~ 20.
PCR pipe standing groove on described base is identical with the application of sample number of recesses do not absorbed water on the application of sample scraps of paper, and the quantity of PCR pipe standing groove and application of sample groove is 8, and the separation on PCR pipe standing groove is identical with the separation of application of sample groove.
This PCR primer electrophoresis Quick-Point sampling device also includes connecting rod, and described N number of application of sample scraps of paper fixed bar is all connected with connecting rod.
Owing to adopting said structure, the present invention has following beneficial effect:
1, base of the present invention is provided with multiple PCR pipe standing groove, eight connecting legs can be placed, do not absorb water simultaneously and the application of sample scraps of paper are arranged multiple application of sample grooves of respective amount, the employing volley of rifle fire can simultaneously from eight connecting leg point samples in the multiple application of sample grooves do not absorbed water the application of sample scraps of paper, namely once can with time point 8 samples, decrease point sample operation, improve work efficiency.
2, the present invention is provided with multiple application of sample scraps of paper fixed bar, the application of sample scraps of paper that can simultaneously multiple do not absorbed water are fixed on base and carry out point sample, do not absorb water the application of sample scraps of paper owing to there being being fixed of application of sample scraps of paper fixed bar, after application of sample or in application of sample process, sample not easily moves, efficiently solve the problem that sample is easily chaotic, prevent from causing sample situation that is chaotic and that pollute to occur because sample mixes.
3, multiple application of sample scraps of paper fixed bar of the present invention is connected on connecting rod simultaneously, is easy to open and depresses multiple application of sample scraps of paper fixed bar, easy to use.
Below, in conjunction with the accompanying drawings and embodiments the technical characteristic of the PCR primer electrophoresis Quick-Point sampling device of the present invention is further described.
Accompanying drawing explanation
Fig. 1: the PCR primer electrophoresis Quick-Point sampling device structural representation of the present invention.
Fig. 2: the application of sample scraps of paper structural representation that do not absorb water of the present invention.
Fig. 3: the understructure schematic diagram of the present invention.
Fig. 4: the base stereographic map of the present invention.
The A-A cut-open view of Fig. 5: Fig. 1.
The B portion enlarged drawing of Fig. 6: Fig. 5.
In figure: 1-base, 2-does not absorb water the application of sample scraps of paper, 21-application of sample groove, 3-PCR pipe standing groove, 4-application of sample scraps of paper fixed bar, 5-connecting rod.
Embodiment
Embodiment 1: a kind of PCR primer electrophoresis Quick-Point sampling device, as shown in figs 1 to 6, comprise base 1 and the application of sample scraps of paper 2 that do not absorb water, described base 1 is provided with multiple PCR pipe standing groove 3, described base 1 is flexibly connected and is provided with 8 application of sample scraps of paper fixed bars 4, namely application of sample scraps of paper fixed bar 4 can lift and depress, the described application of sample scraps of paper 2 that do not absorb water are provided with multiple application of sample groove 21, the described application of sample scraps of paper 2 that do not absorb water are provided with breach 22 near a jiao of application of sample scraps of paper fixed bar link, the application of sample scraps of paper 2 that do not absorb water are pressed abd fixed on base 1 by scraps of paper fixed bar 4.The breach 22 of the application of sample scraps of paper 2 of not absorbing water is corresponding with application of sample scraps of paper fixed bar 4 link shape, and the application of sample scraps of paper 2 that make not absorb water can be pressed abd fixed on base 1 by scraps of paper fixed bar 4 more easily.
In the present embodiment, PCR pipe standing groove 3 on described base is identical with application of sample groove 21 quantity do not absorbed water on the application of sample scraps of paper, pitch of holes on PCR pipe standing groove 3 is identical with the pitch of holes of application of sample groove 21, and the quantity of PCR pipe standing groove and application of sample groove is 8, can place eight connecting legs.As one conversion, described PCR pipe standing groove also can not be identical with the quantity of application of sample groove, and the quantity of PCR pipe standing groove and application of sample groove can increase or reduce as required.
In the present embodiment, the application of sample scraps of paper 2 that do not absorb water are for single use.
In the present embodiment, this PCR primer electrophoresis Quick-Point sampling device also includes connecting rod 5, and described N number of application of sample scraps of paper fixed bar 4 is all connected with connecting rod 5, is convenient to open simultaneously and depress multiple application of sample scraps of paper fixed bar 4.As one conversion, also connecting rod 5 can not be set, make multiple application of sample scraps of paper fixed bar 4 open separately and to depress.
One as the present embodiment converts, and the quantity of application of sample scraps of paper fixed bar 4 can increase according to actual needs or reduce, and is generally 3 ~ 20.
Mechanism: first eight connecting legs are placed in PCR pipe standing groove 3, then the application of sample scraps of paper 2 that will not absorb water are pressed abd fixed on base 1 by scraps of paper fixed bar 4, adopt the volley of rifle fire simultaneously from eight connecting leg point samples in the multiple application of sample grooves do not absorbed water the application of sample scraps of paper, once can with time point 8 samples, after point sample terminates, scraps of paper fixed bar 4 is opened, the excellent application of sample scraps of paper 2 that do not absorb water will be put and take out.
Claims (3)
1. a PCR primer electrophoresis Quick-Point sampling device, it is characterized in that: comprise base (1) and the application of sample scraps of paper (2) that do not absorb water, described base (1) is provided with multiple PCR pipe standing groove (3), the upper flexible connection of described base (1) is provided with N number of application of sample scraps of paper fixed bar (4), the described application of sample scraps of paper (2) that do not absorb water are provided with multiple application of sample groove (21), the described application of sample scraps of paper (2) that do not absorb water are provided with breach (22) near a jiao of application of sample scraps of paper fixed bar link, the application of sample scraps of paper (2) that do not absorb water are pressed abd fixed on base (1) by scraps of paper fixed bar (4), the value of described N is 3 ~ 20.
2. PCR primer electrophoresis Quick-Point sampling device according to claim 1, it is characterized in that: the PCR pipe standing groove (3) on described base is identical with application of sample groove (21) quantity do not absorbed water on the application of sample scraps of paper, the quantity of PCR pipe standing groove and application of sample groove is 8, and the separation on PCR pipe standing groove (3) is identical with the separation of application of sample groove (21).
3. PCR primer electrophoresis Quick-Point sampling device according to claim 1 and 2, is characterized in that: this PCR primer electrophoresis Quick-Point sampling device also includes connecting rod (5), and described N number of application of sample scraps of paper fixed bar (4) is all connected with connecting rod (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510650641.6A CN105259240B (en) | 2015-10-10 | 2015-10-10 | The quick spot sample device of PCR primer electrophoresis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510650641.6A CN105259240B (en) | 2015-10-10 | 2015-10-10 | The quick spot sample device of PCR primer electrophoresis |
Publications (2)
| Publication Number | Publication Date |
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| CN105259240A true CN105259240A (en) | 2016-01-20 |
| CN105259240B CN105259240B (en) | 2017-09-22 |
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| CN201510650641.6A Active CN105259240B (en) | 2015-10-10 | 2015-10-10 | The quick spot sample device of PCR primer electrophoresis |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2258618Y (en) * | 1996-02-12 | 1997-07-30 | 中国人民解放军第四军医大学 | Sample trough for multi-function PCR gene amplification instrument |
| CN2564585Y (en) * | 2002-07-15 | 2003-08-06 | 陕西西大北美基因股份有限公司 | Biochip hybridizing device |
| US20100200405A1 (en) * | 2009-02-09 | 2010-08-12 | Thomas Lenz | Devices, systems and methods for separating magnetic particles |
| CN201990685U (en) * | 2011-03-31 | 2011-09-28 | 杭州金源生物技术有限公司 | Novel compound PCR (polymerase chain reaction) plate |
| US20140370593A1 (en) * | 2011-12-08 | 2014-12-18 | Duke University | Flow chamber assembly and methods of using the same |
| CN205301234U (en) * | 2015-10-10 | 2016-06-08 | 柳州市妇幼保健院 | Quick sample application device of PCR result electrophoresis |
-
2015
- 2015-10-10 CN CN201510650641.6A patent/CN105259240B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2258618Y (en) * | 1996-02-12 | 1997-07-30 | 中国人民解放军第四军医大学 | Sample trough for multi-function PCR gene amplification instrument |
| CN2564585Y (en) * | 2002-07-15 | 2003-08-06 | 陕西西大北美基因股份有限公司 | Biochip hybridizing device |
| US20100200405A1 (en) * | 2009-02-09 | 2010-08-12 | Thomas Lenz | Devices, systems and methods for separating magnetic particles |
| CN201990685U (en) * | 2011-03-31 | 2011-09-28 | 杭州金源生物技术有限公司 | Novel compound PCR (polymerase chain reaction) plate |
| US20140370593A1 (en) * | 2011-12-08 | 2014-12-18 | Duke University | Flow chamber assembly and methods of using the same |
| CN205301234U (en) * | 2015-10-10 | 2016-06-08 | 柳州市妇幼保健院 | Quick sample application device of PCR result electrophoresis |
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|---|---|
| CN105259240B (en) | 2017-09-22 |
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