CN105754854A - Gastric cancer cell culture instrument - Google Patents
Gastric cancer cell culture instrument Download PDFInfo
- Publication number
- CN105754854A CN105754854A CN201610190102.3A CN201610190102A CN105754854A CN 105754854 A CN105754854 A CN 105754854A CN 201610190102 A CN201610190102 A CN 201610190102A CN 105754854 A CN105754854 A CN 105754854A
- Authority
- CN
- China
- Prior art keywords
- test tube
- casing
- cylinder
- adapter
- controller
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 208000005718 Stomach Neoplasms Diseases 0.000 title claims abstract description 16
- 206010017758 gastric cancer Diseases 0.000 title claims abstract description 16
- 201000011549 stomach cancer Diseases 0.000 title claims abstract description 16
- 238000004113 cell culture Methods 0.000 title abstract description 6
- 239000007788 liquid Substances 0.000 claims abstract description 38
- 239000000523 sample Substances 0.000 claims abstract description 20
- 229910000831 Steel Inorganic materials 0.000 claims abstract description 17
- 239000010959 steel Substances 0.000 claims abstract description 17
- 230000005520 electrodynamics Effects 0.000 claims description 13
- 238000009434 installation Methods 0.000 claims 1
- 239000002699 waste material Substances 0.000 abstract description 4
- 238000001802 infusion Methods 0.000 abstract 1
- 239000012531 culture fluid Substances 0.000 description 15
- 239000013589 supplement Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
Abstract
The invention discloses a gastric cancer cell culture instrument and belongs to the technical field of medical instruments.The gastric cancer cell culture instrument comprises a turning cover, a liquid storage box, a steel tube, an electric valve, a first soft tube, a first tube line connector, a linear motor, a first connecting tube, a motor, a moving block, an electric push rod, a nut, a first cylinder, a machine base, threaded rods, a clamping plate, a first electromagnet, a base, a waste liquid box, an infusion pump, a second tube line connector, a second connecting tube, a controller, second electromagnets, a PH probe, a second cylinder, a multi-stage telescopic rod, a machine shell, a magnetic ball and a test tube.The machine shell is provided with the turning cover, the base is installed on the bottom in the machine shell, the test tube is placed on the base, the base and the test tube are movably connected, the test tube is internally provided with the magnetic ball, the threaded rods are installed on the left side and the right side of the test tube respectively, the second electromagnets are installed on the inner side wall of the test tube, and the first electromagnet is installed on the bottom in the test tube.The gastric cancer cell culture instrument has the advantage that cells in culture liquid can be distributed evenly.
Description
Technical field
The present invention relates to a kind of stomach cancer cell and cultivate instrument, belong to technical field of medical instruments.
Background technology
Current stomach cancer cell is cultivated and is mostly adopted traditional cell culture system to cultivate, complicated operation, takies manpower more, and the training method of the stomach cancer cell of prior art is that sleep mode is cultivated mostly, make the cell distribution in culture fluid uneven, affect the accuracy of follow-up test.In order to solve above-mentioned difficulties, it is necessary to develop and a the uniform stomach cancer cell of the cell distribution in culture fluid can be made to cultivate instrument.
Summary of the invention
It is an object of the invention to provide a kind of stomach cancer cell and cultivate instrument.
The problem to be solved in the present invention be prior art cell culture system in the uneven problem of cell distribution in culture fluid.
For realizing the purpose of the present invention, the technical solution used in the present invention is:
nullA kind of stomach cancer cell cultivates instrument，Including renovating、Reserving liquid tank、Steel pipe、Electrodynamic valve、First flexible pipe、First pipe jointer、Linear electric motors、First adapter、Motor、Movable block、Electric pushrod、Nut、First cylinder、Support、Screw rod、Clamping plate、First electric magnet、Base、Exhausted bath box、Drawing liquid pump、Second pipe adapter、Second adapter、Controller、Second electric magnet、PH probe、Second cylinder、Multistage retractable rod、Casing、Magnetic ball and test tube，Described casing is installed and renovates，Casing inner bottom part mounting seat，Holding test tubes on base，Base and test tube are not fixedly linked，Magnetic ball it is provided with in test tube，Screw rod each side installed by test tube，Test tube medial wall is installed more than one the second electric magnet，Test tube inner bottom part installs the first electric magnet，Magnetic ball is positioned at test tube，First adapter is installed on the left of test tube，First adapter is connected by the first pipe jointer and the first flexible pipe，First flexible pipe is arranged on steel pipe，Casing is stretched out in steel pipe upper end，Steel pipe upper end is arranged on reserving liquid tank，Reserving liquid tank is arranged in casing，Steel pipe is installed electrodynamic valve，Second adapter is installed on the right side of test tube，Second adapter is connected with the pipe that is drawn into of drawing liquid pump by second pipe adapter，Drawing liquid pump is arranged in casing，The discharge pipe of drawing liquid pump is connected with exhausted bath box，Exhausted bath box is arranged on the right side of casing，Support is each side installed in casing，Support is installed the first cylinder，The piston rod of the first cylinder is installed clamping plate，Clamping plate and test tube are not fixedly linked，Linear electric motors are each side installed in casing，Linear electric motors are installed movable block，Movable block is installed electric pushrod，Motor is installed in electric pushrod front end，The output shaft of motor is installed nut，In casing, the second cylinder is installed on right side，The piston rod of the second cylinder is installed multistage retractable rod，PH probe is installed under multistage retractable rod，Controller is arranged on the right side of casing，Controller by wire respectively with motor、Linear electric motors、Electric pushrod、Multistage retractable rod、Drawing liquid pump、Electrodynamic valve、First electric magnet and the second electric magnet are connected.
Built-in first electromagnetic valve of described first cylinder, the first electromagnetic valve is connected with controller by wire.
Built-in second electromagnetic valve of described second cylinder, the second electromagnetic valve is connected with controller by wire.
The invention have the advantage that to open and renovate, cell strain is put in test tube；Controller controls electrodynamic valve and opens, and makes reserving liquid tank carry culture fluid to test tube；Controller controls the first cylinder and does stretching motion, makes clamping plate move forward, and makes clamping plate clamp test tube；Controller controls the first electric magnet and the second electric magnet is separately energized, and makes magnetic ball in vitro move up and down, and makes culture fluid upper and lower convection current occur, makes invisible spectro cell distribution uniform；Controller controls the second cylinder and does stretching motion, makes PH probe move forward, and makes PH probe move on test tube；Controller controls multistage retractable rod and does stretching, makes PH probe enter in test tube, decides whether to supplement new culture fluid in test tube according to the result that PH probe is measured；After cell has been cultivated, controller controls electric pushrod and does stretching motion, makes motor and nut move forward, and makes nut close to screw rod；Controller controls electric motor starting, makes nut rotate on screw rod；Controller controls the first cylinder and does contractile motion, makes clamping plate depart from test tube wall；Controller controls linear electric motors and starts, and makes movable block move up, makes test tube move up, facilitates doctor to extract invisible spectro culture fluid and cell；Controller controls drawing liquid pump and starts, and makes invisible spectro waste liquid enter in exhausted bath box.
Accompanying drawing explanation
Fig. 1 is that one stomach cancer cell of the present invention cultivates instrument overall structure front view；
Fig. 2 is clamping plate top views when clamping test tube；
In figure: 1, renovate 2, reserving liquid tank 3, steel pipe 4, electrodynamic valve 5, first flexible pipe 6, first pipe jointer 7, linear electric motors 8, first adapter 9, motor 10, movable block 11, electric pushrod 12, nut 13, first cylinder 14, support 15, screw rod 16, clamping plate 17, first electric magnet 18, base 19, exhausted bath box 20, drawing liquid pump 21, second pipe adapter 22, second adapter 23, controller 24, second electric magnet 25, PH probe 26, second cylinder 27, multistage retractable rod 28, casing 29, magnetic ball 30, test tube.
Detailed description of the invention
Below in conjunction with drawings and Examples, the present invention is further illustrated.
One stomach cancer cell of the present invention cultivates instrument, including renovating 1, reserving liquid tank 2, steel pipe 3, electrodynamic valve 4, first flexible pipe 5, first pipe jointer 6, linear electric motors 7, first adapter 8, motor 9, movable block 10, electric pushrod 11, nut 12, first cylinder 13, support 14, screw rod 15, clamping plate 16, first electric magnet 17, base 18, exhausted bath box 19, drawing liquid pump 20, second pipe adapter 21, second adapter 22, controller 23, second electric magnet 24, PH probe 25, second cylinder 26, multistage retractable rod 27, casing 28, magnetic ball 29 and test tube 30, described casing 28 is installed and renovates 1, open and renovate 1, cell strain is put in test tube 30；Casing 28 inner bottom part mounting seat 18, holding test tubes 30 on base 18, base 18 and test tube 30 are not fixedly linked, magnetic ball 29 it is provided with in test tube 30, screw rod 15 each side installed by test tube 30, test tube 30 medial wall is installed more than one the second electric magnet 24, test tube 30 inner bottom part installs the first electric magnet 17, magnetic ball 29 is positioned at test tube 30, controller 23 controls the first electric magnet 17 and the second electric magnet 24 is separately energized, make magnetic ball 29 move up and down in test tube 30, make culture fluid that upper and lower convection current to occur, make the cell distribution in test tube 30 uniform；First adapter 8 is installed on the left of test tube 30, first adapter 8 is connected with the first flexible pipe 5 by the first pipe jointer 6, first flexible pipe 5 is arranged on steel pipe 3, casing 28 is stretched out in steel pipe 3 upper end, steel pipe 3 upper end is arranged on reserving liquid tank 2, and reserving liquid tank 2 is arranged in casing 28, and steel pipe 3 is installed electrodynamic valve 4, controller 23 controls electrodynamic valve 4 and opens, and makes reserving liquid tank 2 carry culture fluid to test tube 30；Second adapter 22 is installed on the right side of test tube 30, second adapter 22 is connected with the pipe that is drawn into of drawing liquid pump 20 by second pipe adapter 21, drawing liquid pump 20 is arranged in casing 28, the discharge pipe of drawing liquid pump 20 is connected with exhausted bath box 19, exhausted bath box 19 is arranged on the right side of casing 28, controller 23 controls drawing liquid pump 20 and starts, the waste liquid in test tube 30 is made to enter in exhausted bath box 19, support 14 is each side installed in casing 28, support 14 is installed the first cylinder 13, first built-in first electromagnetic valve of cylinder 13, first electromagnetic valve is connected with controller 23 by wire, controller 23 controls the first cylinder 13 and does stretching motion, clamping plate 16 are made to move forward, clamping plate 16 are made to clamp test tube 30；Controller 23 controls the first cylinder 13 and does contractile motion, makes clamping plate 16 depart from test tube 30 wall；Installing clamping plate 16 on the piston rod of the first cylinder 13, clamping plate 16 and test tube 30 are not fixedly linked, and each side install linear electric motors 7 in casing 28, and controller 23 controls motor 9 and starts, and makes nut 12 rotate on screw rod 15；Installing movable block 10 on linear electric motors 7, controller 23 controls linear electric motors 7 and starts, and makes movable block 10 move up, and makes test tube 30 move up, and facilitates doctor to extract the culture fluid in test tube 30 and cell；Installing electric pushrod 11 on movable block 10, after cell has been cultivated, controller 23 controls electric pushrod 11 and does stretching motion, makes motor 9 and nut 12 move forward, and makes nut 12 close to screw rod 15；Motor 9 is installed in electric pushrod 11 front end, the output shaft of motor 9 is installed nut 12, in casing 28, the second cylinder 26 is installed on right side, second built-in second electromagnetic valve of cylinder 26, second electromagnetic valve is connected with controller 23 by wire, controller 23 controls the second cylinder 26 and does stretching motion, makes PH probe 25 move forward, and makes PH probe 25 move on test tube 30；Installing multistage retractable rod 27 on the piston rod of the second cylinder 26, controller 23 controls multistage retractable rod 27 and does stretching, makes PH probe 25 enter in test tube 30, decides whether to supplement new culture fluid in test tube 30 according to the result that PH probe 25 is measured；Multistage retractable rod installs PH probe 25 27 times, controller 23 is arranged on the right side of casing 28, and controller 23 is connected with motor 9, linear electric motors 7, electric pushrod 11, multistage retractable rod 27, drawing liquid pump 20, electrodynamic valve the 4, first electric magnet 17 and the second electric magnet 24 respectively by wire.
Using method of the present invention: open and renovate 1, puts into cell strain in test tube 30；Controller 23 controls electrodynamic valve 4 and opens, and makes reserving liquid tank 2 carry culture fluid to test tube 30；Controller 23 controls the first cylinder 13 and does stretching motion, makes clamping plate 16 move forward, and makes clamping plate 16 clamp test tube 30；Controller 23 controls the first electric magnet 17 and the second electric magnet 24 is separately energized, and makes magnetic ball 29 move up and down in test tube 30, makes culture fluid upper and lower convection current occur, makes the cell distribution in test tube 30 uniform；Controller 23 controls the second cylinder 26 and does stretching motion, makes PH probe 25 move forward, and makes PH probe 25 move on test tube 30；Controller 23 controls multistage retractable rod 27 and does stretching, makes PH probe 25 enter in test tube 30, decides whether to supplement new culture fluid in test tube 30 according to the result that PH probe 25 is measured；After cell has been cultivated, controller 23 controls electric pushrod 11 and does stretching motion, makes motor 9 and nut 12 move forward, and makes nut 12 close to screw rod 15；Controller 23 controls motor 9 and starts, and makes nut 12 rotate on screw rod 15；Controller 23 controls the first cylinder 13 and does contractile motion, makes clamping plate 16 depart from test tube 30 wall；Controller 23 controls linear electric motors 7 and starts, and makes movable block 10 move up, and makes test tube 30 move up, and facilitates doctor to extract the culture fluid in test tube 30 and cell；Controller 23 controls drawing liquid pump 20 and starts, and makes the waste liquid in test tube 30 enter in exhausted bath box 19.
Claims (3)
- null1. a stomach cancer cell cultivates instrument，Including renovating (1)、Reserving liquid tank (2)、Steel pipe (3)、Electrodynamic valve (4)、First flexible pipe (5)、First pipe jointer (6)、Linear electric motors (7)、First adapter (8)、Motor (9)、Movable block (10)、Electric pushrod (11)、Nut (12)、First cylinder (13)、Support (14)、Screw rod (15)、Clamping plate (16)、First electric magnet (17)、Base (18)、Exhausted bath box (19)、Drawing liquid pump (20)、Second pipe adapter (21)、Second adapter (22)、Controller (23)、Second electric magnet (24)、PH probe (25)、Second cylinder (26)、Multistage retractable rod (27)、Casing (28)、Magnetic ball (29) and test tube (30)，It is characterized in that: (1) is renovated in the upper installation of described casing (28)，Casing (28) inner bottom part mounting seat (18)，The upper holding test tubes (30) of base (18)，Base (18) and test tube (30) are not fixedly linked，Magnetic ball (29) it is provided with in test tube (30)，Screw rod (15) each side installed by test tube (30)，Test tube (30) medial wall is installed more than one the second electric magnet (24)，Test tube (30) inner bottom part installs the first electric magnet (17)，Magnetic ball (29) is positioned at test tube (30)，The first adapter (8) is installed in test tube (30) left side，First adapter (8) is connected with the first flexible pipe (5) by the first pipe jointer (6)，First flexible pipe (5) is arranged on steel pipe (3)，Casing (28) is stretched out in steel pipe (3) upper end，Steel pipe (3) upper end is arranged on reserving liquid tank (2)，Reserving liquid tank (2) is arranged in casing (28)，Steel pipe (3) is installed electrodynamic valve (4)，The second adapter (22) is installed on test tube (30) right side，Second adapter (22) is connected with the pipe that is drawn into of drawing liquid pump (20) by second pipe adapter (21)，Drawing liquid pump (20) is arranged in casing (28)，The discharge pipe of drawing liquid pump (20) is connected with exhausted bath box (19)，Exhausted bath box (19) is arranged on casing (28) right side，Support (14) is each side installed in casing (28)，Support (14) is upper installs the first cylinder (13)，The piston rod of the first cylinder (13) is installed clamping plate (16)，Clamping plate (16) and test tube (30) are not fixedly linked，Linear electric motors (7) are each side installed in casing (28)，Linear electric motors (7) are installed movable block (10)，Movable block (10) is installed electric pushrod (11)，Motor (9) is installed in electric pushrod (11) front end，The output shaft of motor (9) is installed nut (12)，In casing (28), the second cylinder (26) is installed on right side，The piston rod of the second cylinder (26) is installed multistage retractable rod (27)，PH probe (25) is installed under multistage retractable rod (27)，Controller (23) is arranged on casing (28) right side，Controller (23) by wire respectively with motor (9)、Linear electric motors (7)、Electric pushrod (11)、Multistage retractable rod (27)、Drawing liquid pump (20)、Electrodynamic valve (4)、First electric magnet (17) is connected with the second electric magnet (24).
- 2. a kind of stomach cancer cell according to claim 1 cultivates instrument, it is characterized in that: described built-in first electromagnetic valve of first cylinder (13), and the first electromagnetic valve is connected with controller (23) by wire.
- 3. a kind of stomach cancer cell according to claim 1 cultivates instrument, it is characterized in that: described built-in second electromagnetic valve of second cylinder (26), and the second electromagnetic valve is connected with controller (23) by wire.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610190102.3A CN105754854B (en) | 2016-03-30 | 2016-03-30 | A kind of stomach cancer cell culture instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610190102.3A CN105754854B (en) | 2016-03-30 | 2016-03-30 | A kind of stomach cancer cell culture instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105754854A true CN105754854A (en) | 2016-07-13 |
| CN105754854B CN105754854B (en) | 2017-11-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610190102.3A Expired - Fee Related CN105754854B (en) | 2016-03-30 | 2016-03-30 | A kind of stomach cancer cell culture instrument |
Country Status (1)
| Country | Link |
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| CN (1) | CN105754854B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107384791A (en) * | 2017-08-22 | 2017-11-24 | 江苏省肿瘤医院 | Stomach cancer cell incubator |
| CN111500447A (en) * | 2020-04-26 | 2020-08-07 | 海口健康岛生物科技有限公司 | Cell culture device for immune cell amplification |
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| US5443791A (en) * | 1990-04-06 | 1995-08-22 | Perkin Elmer - Applied Biosystems Division | Automated molecular biology laboratory |
| JP2006000105A (en) * | 2004-06-16 | 2006-01-05 | Sunao Otake | Culture apparatus for applying pressure and stimulation of pulsatile flow |
| CN1912627A (en) * | 2006-08-23 | 2007-02-14 | 陕西西大北美基因股份有限公司 | Blood treatment working station based on micronano magnetic particle and its control method |
| CN103298922A (en) * | 2011-03-29 | 2013-09-11 | 南京新诺丹生物技术有限公司 | Multifunctional bioreactor system and methods for cell sorting and culturing |
| CN104694474A (en) * | 2015-03-31 | 2015-06-10 | 南京新诺丹生物技术有限公司 | Cell culture method |
| CN105087378A (en) * | 2015-09-02 | 2015-11-25 | 舟山医院 | Magnetic bead sorting instrument |
| CN105121621A (en) * | 2012-10-18 | 2015-12-02 | 张永新 | Bioreactor system and methods for alternative cell culture between static and dynamic |
-
2016
- 2016-03-30 CN CN201610190102.3A patent/CN105754854B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5443791A (en) * | 1990-04-06 | 1995-08-22 | Perkin Elmer - Applied Biosystems Division | Automated molecular biology laboratory |
| JP2006000105A (en) * | 2004-06-16 | 2006-01-05 | Sunao Otake | Culture apparatus for applying pressure and stimulation of pulsatile flow |
| CN1912627A (en) * | 2006-08-23 | 2007-02-14 | 陕西西大北美基因股份有限公司 | Blood treatment working station based on micronano magnetic particle and its control method |
| CN103298922A (en) * | 2011-03-29 | 2013-09-11 | 南京新诺丹生物技术有限公司 | Multifunctional bioreactor system and methods for cell sorting and culturing |
| CN105121621A (en) * | 2012-10-18 | 2015-12-02 | 张永新 | Bioreactor system and methods for alternative cell culture between static and dynamic |
| CN104694474A (en) * | 2015-03-31 | 2015-06-10 | 南京新诺丹生物技术有限公司 | Cell culture method |
| CN105087378A (en) * | 2015-09-02 | 2015-11-25 | 舟山医院 | Magnetic bead sorting instrument |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107384791A (en) * | 2017-08-22 | 2017-11-24 | 江苏省肿瘤医院 | Stomach cancer cell incubator |
| CN111500447A (en) * | 2020-04-26 | 2020-08-07 | 海口健康岛生物科技有限公司 | Cell culture device for immune cell amplification |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105754854B (en) | 2017-11-24 |
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Effective date of registration: 20220413 Address after: 102600 Room 403, unit 1, 15th floor, changfengyuan, Huangcun Town, Daxing District, Beijing Patentee after: Du Lihong Address before: 316021 No. 739, dingshen Road, Lincheng street, Dinghai District, Zhoushan City, Zhejiang Province Patentee before: ZHOUSHAN Hospital |
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