CN105772121A - Application of microfluidic technology in blood separation - Google Patents
Application of microfluidic technology in blood separation Download PDFInfo
- Publication number
- CN105772121A CN105772121A CN201610130458.8A CN201610130458A CN105772121A CN 105772121 A CN105772121 A CN 105772121A CN 201610130458 A CN201610130458 A CN 201610130458A CN 105772121 A CN105772121 A CN 105772121A
- Authority
- CN
- China
- Prior art keywords
- blood
- application
- separate
- control technique
- blood flow
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
Abstract
The invention discloses application of a microfluidic technology in blood separation, a microflow multilayer blood flow channel is designed, and blood can be separated into an upper layer and a lower layer naturally under the gravity action by the aid of different own weight ratios of plasma and blood cells.According to the application of the microfluidic technology in blood separation, blood separation equipment conditions are not needed, the plasma can be separated, great convenience can be provided for blood analysis on the site, the microfluidic technology can be used for self health diagnose at home and has a great application value on aviation medicine.
Description
Technical field
The present invention relates to microfluidic art, the application in separating in blood particularly to a kind of microflow control technique.
Background technology
Being one of requisite project to blood component analysis in clinical diagnosis project, blood cell, blood plasma must be easily separated by the analysis of blood.Blood plasma isolation technics: respectively by filter membrane and centrifugation two ways, venous samples can sampling to add the materials such as anticoagulant, EDTA, sodium citrate.
Centrifugation: gathering blood sample very little, can not separate and need 15-30 minute；
Filter type: the equipment such as filter membrane and vacuum pump will be adopted.
Summary of the invention
In order to overcome drawbacks described above, the invention provides the application during a kind of microflow control technique overcoming the weak points such as blood plasma disengaging time length, blood sampling volume be big separates in blood.
The present invention is to solve its technical problem be the technical scheme is that a kind of microflow control technique separate in blood in application, the blood flow passage of design miniflow multilamellar, utilize blood plasma different from blood cell own wt ratio, make blood isolate blood cell and blood plasma in blood flow passage under gravity.
As a further improvement on the present invention, described blood flow passage adopts the transparent double face glue material of good biocompatibility, thickness 30-50 micron laminated, forms with 266nm wavelength laser light width 20-100 micron engraving.
As a further improvement on the present invention, multilamellar is engraved as microchannel and is bonded on transparent plastic sheet, with vacuum pressing-combining machine pressing or 100 DEG C-300 DEG C pressure 5Kg/cm of heat bondable material2Pressurization is bonded to a microchannel that one is closed, that circulation is good, it is prevented that dust enters, it is necessary to complete in clean bench.
As a further improvement on the present invention, described blood flow channel depth is between 30-200uL, and width is between 30-300uL.
The invention has the beneficial effects as follows: the microflow control technique of the present invention separate in blood in application without blood separation device condition, blood plasma can be completed separate, hemanalysis can be provided great convenience by scene, it is also possible in autognostic at home is healthy and uses in aviation medicine and has very big practical value.
Accompanying drawing explanation
Fig. 1 is present configuration schematic diagram；
Fig. 2 is the close-up schematic view of the present invention；
Fig. 3 is principles of the invention schematic diagram.
Detailed description of the invention
In order to deepen the understanding of the present invention, below in conjunction with embodiment and accompanying drawing, the invention will be further described, and this embodiment is only used for explaining the present invention, is not intended that limiting the scope of the present invention.
Fig. 1 illustrate a kind of microflow control technique of the present invention separate in blood in a kind of embodiment of application, the blood flow passage of design miniflow multilamellar, its ditch depth 30-200uL, between furrow width 30-300uL, overall channel is within 50mm, at the transparent material that biocompatibility is good, as: polyimides, PEN, PET, thickness 30-50 micron laminated, form with 266nm wavelength laser light width 20-100 micron engraving, multilamellar is engraved as microchannel and is bonded on transparent plastic sheet, with vacuum pressing-combining machine pressing or 100 DEG C-300 DEG C pressure 5Kg/cm of heat bondable material2Pressurization is bonded to a microchannel that one is closed, that circulation is good, it is prevented that dust enters, it is necessary to completing in clean bench, described blood flow channel depth is between 30-200uL, and width is between 30-300uL.
Peripheral blood instills the liquid inlet of blood flow passage, then by blood flow passage vertically downward, liquid inlet placement parallel to the ground, after several minutes, blood plasma can well be separated, in blood flow passage, separated plasma is as shown in Figure 3, blood is separated into two layers under gravity, upper strata is blood plasma, lower floor is blood cell, droplet of blood becomes a mandarin after the end of crossing, and by blood flow passage vertically downward, in the short time, trace blood plasma can be separated, the separated plasma time is that within 1 minute, blood does not find solidification phenomenon.
Claims (4)
1. a microflow control technique separate in blood in application, it is characterised in that: design miniflow multilamellar blood flow passage, utilize blood plasma different from blood cell own wt ratio, make blood isolate blood cell and blood plasma in blood flow passage under gravity.
2. microflow control technique according to claim 1 separate in blood in application, it is characterized in that: described blood flow passage adopts the transparent double face glue material of good biocompatibility, thickness 30-50 micron laminated, forms with 266nm wavelength laser light width 20-100 micron engraving.
3. microflow control technique according to claim 2 separate in blood in application, it is characterised in that: multilamellar is engraved as microchannel and is bonded on transparent plastic sheet, with vacuum pressing-combining machine pressing or 100 DEG C-300 DEG C pressure 5Kg/cm of heat bondable material2Pressurization is bonded to a microchannel that one is closed, that circulation is good, it is prevented that dust enters, it is necessary to complete in clean bench.
4. microflow control technique according to claim 1 separate in blood in application, it is characterised in that: described blood flow channel depth is between 30-200uL, and width is between 30-300uL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610130458.8A CN105772121A (en) | 2016-03-09 | 2016-03-09 | Application of microfluidic technology in blood separation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610130458.8A CN105772121A (en) | 2016-03-09 | 2016-03-09 | Application of microfluidic technology in blood separation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105772121A true CN105772121A (en) | 2016-07-20 |
Family
ID=56387862
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610130458.8A Pending CN105772121A (en) | 2016-03-09 | 2016-03-09 | Application of microfluidic technology in blood separation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105772121A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004027391A1 (en) * | 2002-09-20 | 2004-04-01 | Japan Science And Technology Agency | Blood analyzer and method of separating plasma |
| US20060078873A1 (en) * | 2003-02-19 | 2006-04-13 | Hiroki Ogawa | Blood analysis device and blood analysis method |
| US20120152858A1 (en) * | 2010-12-15 | 2012-06-21 | Abbott Laboratories | Apparatus and method for separation of whole blood into plasma or serum and cells |
| CN102631959A (en) * | 2012-04-19 | 2012-08-15 | 南京大学 | Microfluidic device for realizing continuous separation of blood plasma and separation method blood plasma |
| US20120276641A1 (en) * | 2009-10-30 | 2012-11-01 | Dublin City University | Microfluidic device providing degassing driven fluid flow |
| WO2015044454A2 (en) * | 2013-09-30 | 2015-04-02 | Göran Stemme | A microfluidic device, use and methods |
-
2016
- 2016-03-09 CN CN201610130458.8A patent/CN105772121A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004027391A1 (en) * | 2002-09-20 | 2004-04-01 | Japan Science And Technology Agency | Blood analyzer and method of separating plasma |
| US20060078873A1 (en) * | 2003-02-19 | 2006-04-13 | Hiroki Ogawa | Blood analysis device and blood analysis method |
| US20120276641A1 (en) * | 2009-10-30 | 2012-11-01 | Dublin City University | Microfluidic device providing degassing driven fluid flow |
| US20120152858A1 (en) * | 2010-12-15 | 2012-06-21 | Abbott Laboratories | Apparatus and method for separation of whole blood into plasma or serum and cells |
| CN102631959A (en) * | 2012-04-19 | 2012-08-15 | 南京大学 | Microfluidic device for realizing continuous separation of blood plasma and separation method blood plasma |
| WO2015044454A2 (en) * | 2013-09-30 | 2015-04-02 | Göran Stemme | A microfluidic device, use and methods |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5608943B2 (en) | Plasma separation apparatus and method | |
| US20210260579A1 (en) | Plasma separating microfluidic device | |
| CN205517817U (en) | Prevent little valve of refluence | |
| CN106902904B (en) | For the liquid control valve door gear and its micro-fluidic chip of micro-fluidic chip | |
| CN106124388B (en) | Capillary tube sample injection system, sample injection method and single cell electrical characteristic detection system | |
| DE102011078961A1 (en) | System for separating constituents such as blood plasma from blood sample, has filter layer arranged between substrates for separating the constituents of sample in one cavity and for discharging separated constituents to other cavity | |
| CN106994369A (en) | Regulatable micro-fluidic integrated device of flux and preparation method thereof | |
| JP6566319B2 (en) | Separation unit, separation method, fluid device, composite fluid device and kit | |
| CN104083819B (en) | Precise liquid medicine filter for medical infusion set | |
| CN107377024A (en) | Micro-fluidic syringe filter and its application method | |
| CN110577884A (en) | White blood cell extraction element | |
| CN109679842B (en) | Micro-fluidic chip | |
| CN105772121A (en) | Application of microfluidic technology in blood separation | |
| CN108160126B (en) | Micro-fluidic chip for high-throughput enrichment of micro-particles | |
| CN205517818U (en) | Little valve of choked flow | |
| CN107126753B (en) | Active micro-fluidic chip and whole blood filter equipment thereof | |
| CN201935921U (en) | Colloidal gold whole blood test card with U-shaped filter structure and three layers of blood separating films | |
| CN205580993U (en) | Liquid drop produces device based on micro -fluidic chip and piezoceramics component | |
| CN106955749A (en) | Blood plasma extraction element based on micro-fluidic chip | |
| CN204072113U (en) | Vaccum blood collecting test tube | |
| CN106479868A (en) | A kind of microdevice based on multistage dialysis scavenger-cell cryoprotective agent | |
| CN203802568U (en) | Isolating draining and specimen collecting laminated film special for percutaneous nephrolithotomy surgery | |
| Chen et al. | Characterization of thermoplastic microfiltration chip for the separation of blood plasma from human blood | |
| CN205517821U (en) | Whole blood separating mechanism | |
| CN107349983A (en) | A kind of micro-fluidic device and its separation method realized whole blood blood plasma and persistently separated |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160720 |
|
| RJ01 | Rejection of invention patent application after publication |