CN105777921A - Astragalus polysaccharide extraction process and application thereof - Google Patents
Astragalus polysaccharide extraction process and application thereof Download PDFInfo
- Publication number
- CN105777921A CN105777921A CN201410828015.7A CN201410828015A CN105777921A CN 105777921 A CN105777921 A CN 105777921A CN 201410828015 A CN201410828015 A CN 201410828015A CN 105777921 A CN105777921 A CN 105777921A
- Authority
- CN
- China
- Prior art keywords
- astragalus
- astragalus polysaccharide
- extraction process
- warming
- astragalus polysaccharides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses an astragalus polysaccharide extraction process and application thereof, and relates to the field of natural medicine extraction and application.The astragalus polysaccharide extraction process includes the steps of pretreatment, warm water soaking, enzyme hydrolysis, alcohol precipitation and the like, a cellulase and papain mixed enzymic preparation is adopted to increase an astragalus polysaccharide collecting rate and hydrolysis reaction efficiency, then reaction environment is adjusted by adding a calcium hydroxide solution, and enzymes are inactivated while the astragalus polysaccharide collecting rate is increased.The astragalus polysaccharide extraction process is mild in condition and high in extraction rate, and obtained astragalus polysaccharide extract is high in purity and biological activity and can be used for enhancing body immunity functions, sterilizing, resisting viruses, tumors, osteoporosis and ulcers and adjusting blood pressure serving as a medicine raw material .
Description
Technical field
The invention belongs to natural drug to extract and application, more particularly to a kind of astragalus polysaccharides extraction process and application thereof.
Background technology
The Radix Astragali is a kind of common QI invigorating Chinese medicine, for the dry root of leguminous plant Radix Astagali or Radix Astragali.Sweet in the mouth, slightly warm in nature, return spleen, lung meridian, there is QI invigorating, effect of qi-restoratives.Life can consolidating superficial resistance, toxin expelling, anti-skin ulcer granulation promoting；Process with energy QI invigorating invigorating middle warmer.Its micromicro diuresis is relieved oedema or abdominal distension through diuresis or purgation wet, and the persistent period is longer.The Radix Astragali also has enhancing immune system, resists disease, health invigorating, resisting oxidation and delaying senility, improves cardiac status, antiviral, the function such as anticancer, and its drug effect is notable, is widely used.
Containing astragalus polysaccharides, astragaloside, flavone, several amino acids, trace element, carotene, folic acid isoreactivity composition in the Radix Astragali, all relevant with its drug effect.Modern age, pharmacological research proved, astragalus polysaccharides and astragaloside are the main components of the Radix Astragali, and pharmacological action is extensive.Wherein astragalus polysaccharides is most important natural constituent in the Radix Astragali, has significant effect in enhancing human body immunity and sterilizing and anti-virus, is the class macromolecular compound playing deciding factor in Radix Astragali pharmacological action.
Existing literature search finds, the method of existing extraction astragalus polysaccharides has: decoction and alcohol sedimentation technique, alkali carry alcohol deposition method, ferment treatment method, microwave method, ultrafiltration are sent out, supercritical carbon dioxide process etc., decoction and alcohol sedimentation technique therein and alkali carry alcohol deposition method, extraction ratio is relatively low, purity is not high enough, alkaline extraction also to adjust pH, and alkaline environment may damaged portion bioactive ingredients simultaneously；Microwave method, ultrafiltration, supercritical carbon dioxide process then complex steps, operation require that height, equipment investment are big, be not suitable for factory and extract the Radix Astragali on a large scale.
Chinese patent CN1330082A discloses the extraction separation of a kind of astragalus polysaccharides and astragaloside, wherein adopts backflow, flocculation, ultrafiltration, filtration fat post separation and Extraction, length consuming time, step tired many, have high input；Chinese patent CN101029086A discloses the method that United microwave, microfiltration, ultrafiltration and precipitate with ethanol technology prepare astragalus polysaccharides, although DNA purity is higher, but technology requires high, used device costliness, is unsuitable for current factory's large-scale production Radix Astragali.
Invention currently for astragalus polysaccharides extracting method is reported more, and the present invention, by astragalus polysaccharides extracting method is furtherd investigate, has invented a kind of astragalus polysaccharides extraction process.The method yield is high, economical and energy saving, and the Radix Astragali of extraction has many-sided advantages such as sugar content is big, biological activity is high.
Summary of the invention
One of the object of the invention is the deficiency existed for prior art, it is provided that a kind of astragalus polysaccharides extraction process.This technique adopts the method for warm water leaching, enzyme hydrolysis, alcohol precipitation, and the astragalus polysaccharides yield of extraction is high, sugar content is big, the biological activity height of economical and energy saving and product.
The two of the object of the invention are to provide a kind of astragalus polysaccharides crude drug, can be made into the health product of various dosage form and pharmaceuticals, for enhancing human body immunity and sterilizing and anti-virus.
To achieve these goals, the present invention is achieved by the following technical solutions:
A kind of astragalus polysaccharides extraction process, including step in detail below:
(1) pretreatment: the raw material Radix Astragali is after choosing, wash, moisten, cutting, standby.
(2) raw material enzymolysis: the pretreated Radix Astragali is put in the distilled water of 8-12 times amount, be warming up to 45-50 DEG C, regulate pH5.5-7, add enzyme preparation, be hydrolyzed 2-3h；Add aqua calcis, regulate pH value to 9-10, be warming up to 75 DEG C, after keeping 60-100min, naturally cool to 50 DEG C, filter cleaner, obtain filtrate I, standby；
(3) residue enzymolysis: collect the residue in (2), put in the distilled water of 6-8 times amount, be warming up to 50-60 DEG C, regulate pH5.5-7, add enzyme preparation, be hydrolyzed 2-3h；Add aqua calcis, regulate pH value to 9-10, be warming up to 75 DEG C, after keeping 40-60min, naturally cool to 50 DEG C, filter cleaner, obtain filtrate II-1；Collect residue, repeat above operation, obtain filtrate II-2；Merging filtrate I, II-1 and II-2.
(4) concentration: the filtrate after merging being put in vacuum decker, concentrating under reduced pressure, concentrated solution is the 70% of stock solution.
(5) alcohol analysis: be slowly added into 95% ethanol in concentrated solution, reach 70% to concentration of alcohol, standing sedimentation 8-12h, precipitating thing is astragalus polysaccharides, mechanical filter, then by 95% washing with alcohol 1-2 time.
(6) the astragalus polysaccharides precipitating thing after washing is carried out lyophilization, then pulverize, cross 100 mesh sieves, obtain astragalus polysaccharide extract.
The described Radix Astragali is the Radix Astragali dry root being purchased from Dingxi.
Described profit refers to infiltration medical material so that it is soften penetrating；The present invention adopts medical material to be placed on the moistening mat make of thin bamboo strips dew moistening method of natural moisture absorption softening；Preferably employ the moistening method of the airtight softening of moistening medical material；The vacuum heating method that more preferably moistening medical material softens in medicine processing device.
Described cutting refers to section, and the Radix Astragali is cut in the present invention inclined tab shape, sheet thickness 2.4-3.6mm.
Described enzyme preparation is the mixture of cellulase and papain, for the 1.5%-3.0% of raw material astragalus weight, wherein cellulase 0%-1.5%, papain 0-1.5%.
Described enzyme preparation consumption is preferably the 2.2% of raw material astragalus weight, wherein cellulase 1.2% and papain 1.0%.
One of purposes of described astragalus polysaccharide extract is the crude drug as a kind of health product, is used alone, and adds diluent and prepares into oral liquid, for enhancing human body immunity function, sterilizing and anti-virus, resisting fatigue, delays naturally-aged.
One of purposes of described astragalus polysaccharide extract is the crude drug as a kind of medicine, it is configured to pharmaceutical composition with pharmaceutically acceptable carrier, excipient, diluent, injection, oral liquid, tablets and other formulations is prepared into, for enhancing human body immunity function, sterilizing and anti-virus, antitumor, osteoporosis, antiulcer and adjustment blood pressure etc. with conventional method of Chinese medicinal.
The invention has the beneficial effects as follows:
Compared with prior art, the astragalus polysaccharides extraction process of the present invention, when washing softening, adopts the one in dew moistening method, moistening method, vacuum heating method, avoid the Radix Astragali repeatedly and to soak in water for a long time and lose water soluble polysaccharide, virtually improve polysaccharide extract rate；When enzymolysis, adopt the mixing enzyme preparation of cellulase and papain, improve the efficiency of enzyme digestion reaction and the extraction ratio of astragalus polysaccharides, and to twice enzymolysis of residue, make the polysaccharide in the Radix Astragali extract as far as possible, improve the extraction ratio of astragalus polysaccharides from another point of view；Add aqua calcis and also regulate pH value, making stock solution is alkalescence, while improving extraction ratio, substitutes the mode of high temperature enzyme denaturing activity, it is to avoid the high temperature damage to astragalus polysaccharides activity, makes process conditions become gentle, and energy consumption reduces；During concentration, it is concentrated into the 70% of stock solution, so that amount of alcohol added reduces in alcohol analysis step, reduces process costs.
The process conditions of the present invention are gentle, and extraction ratio and purity are high, have the features such as efficient, safe, energy-saving and cost-reducing, belong to resource-conserving, environmentally friendly friendly process.
Detailed description of the invention
Further the present invention is set forth with experimental example in conjunction with the embodiments, be not construed as limiting the invention.
Embodiment 1
Taking Milkvetch Root 2kg, add 24kg distilled water, temperature rises to 58 DEG C, pH5.5, add cellulase 24g and papain 20g, after keeping 120min, add aqua calcis, regulate pH value to 10, after being warming up to 75 DEG C of maintenance 60min, naturally cooling to 50 DEG C, deslagging filtering, filtrate is standby；Residue puts in 16kg distilled water, is warming up to 58 DEG C, regulates pH5.5, adds enzyme preparation, cellulase 24g and papain 20g, adds aqua calcis after keeping 120min, regulates pH value to 10, after being warming up to 75 DEG C of maintenance 60min, naturally cool to 50 DEG C, deslagging filtering, merging filtrate；Filtrate, to the 70% of material liquid, is settled, mechanical filter by vacuum-concentrcted with 95% ethanol, and precipitating thing is astragalus polysaccharide extract.
With Phenol sulfuric acid procedure, at 500nm place, the content of determined by ultraviolet spectrophotometry total polysaccharides, with glucose meter for 51.8%.
Embodiment 2
Taking Milkvetch Root and be about 3kg, add 30kg distilled water, temperature rises to 55 DEG C, pH6, add cellulase 36g and papain 30g, after keeping 120min, add aqua calcis, regulate pH value to 9, after being warming up to 75 DEG C of maintenance 60min, naturally cooling to 50 DEG C, deslagging filtering, filtrate is standby；Residue puts in 21kg distilled water, is warming up to 55 DEG C, regulates pH6, adds cellulase 36g and papain 30g, add aqua calcis after keeping 120min, regulate pH value to 10, after being warming up to 75 DEG C of maintenance 60min, naturally cool to 50 DEG C, deslagging filtering, merging filtrate；Filtrate, to the 70% of material liquid, is settled, mechanical filter by vacuum-concentrcted with 95% ethanol, and precipitating thing is astragalus polysaccharide extract.
With Phenol sulfuric acid procedure, at 500nm place, the content of determined by ultraviolet spectrophotometry total polysaccharides, with glucose meter for 50.2%.
Embodiment 3
Make aqueous injection
Embodiment 1 gained astragalus polysaccharide extract is further purified separation through agarose gel post sephacrylS300, sephadexG50 chromatograph: obtain purity astragalus polysaccharide extract more than 98%, add appropriate additives, dissolve mixing, filter clarification, by injection technological requirement, embedding in ampoule, sterilizing, thus obtaining the product.
Embodiment 4
Make tablet
Embodiment 2 gained astragalus polysaccharide extract is taken in right amount, adds the right amount of auxiliary materials such as diluent, disintegrating agent, mixing, make granule, dry, tabletted, coating or spray film-coat and get final product.
Experimental example 1
Astragalus polysaccharides is on the immune impact experiment of porkling
Select 24 20 age in days three way cross piglets, be divided into matched group and 3 test group (test group piglet when 20 ages in days, 35 ages in days, 50 age in days respectively intramuscular injection astragalus polysaccharides 2,4,6mL), and respectively at immune indexes such as 20 ages in days, 35 ages in days, 50 ages in days blood sampling detection piglet peripheral blood neutrophil percent, hog cholera antibody level, lymhocyte transformation rates.
Result shows: the astragalus polysaccharides of application doses can significantly improve the antibody horizontal (P < 0.01) of piglet swine Fever Vaccine in pole, significantly improves piglet peripheral blood neutrophil percent and lymhocyte transformation rate (P < 0.05).Show that the immunologic function of piglet is had certain facilitation by astragalus polysaccharides.
Experimental example 2
The impact of chickling antiviral is tested by astragalus polysaccharides
In 50 ages in days, healthy Hai Lanbai chickling is for object of study, by astragalus polysaccharides (APS) by 0mg/, 5mg/
| Group | Dosage/mg | E-C3bRR | ERER | E-ICRR | ERIR |
| Experimental group 1 | 0 | 8 | 11 | 26 | 32 |
| Experimental group 2 | 5 | 15 | 18 | 19 | 21 |
| Experimental group 3 | 10 | 34 | 29 | 13 | 14 |
Only only giving in the way of feeding test chickling with 10mg/, give experiment chickling ND IV is vaccine virus immunization simultaneously.
Result shows, chickling C3b receptor rosette rate (E-C3bRR) and C 3 b receptor of red blood cells garland promotion rate (ERER) significantly raise, red blood cell immune complexes rosett (E-ICRR) and C 3 b receptor of red blood cells garland suppression ratio (ERIR) then reduce, namely APS is remarkably improved the hematid immunity function of chickling, and APS10mg/ only organizes effect and is better than 5mg/ group；Being Seedling immunized chicks for ND IV, with APS10mg/ intramuscular injection, ND IV collunarium eye dripping is the most notable to the potentiation of hematid immunity function.
Claims (4)
1. an astragalus polysaccharides extraction process, it is characterised in that: include step in detail below:
(1) pretreatment: the raw material Radix Astragali is after choosing, wash, moisten, cutting, standby.
(2) raw material enzymolysis: the pretreated Radix Astragali is put in the distilled water of 8-12 times amount, be warming up to 45-50 DEG C, regulate pH5.5-7, add enzyme preparation, be hydrolyzed 2-3h；Add aqua calcis, regulate pH value to 9-10, be warming up to 75 DEG C, after keeping 60-100min, naturally cool to 50 DEG C, filter cleaner, obtain filtrate I, standby；
(3) residue enzymolysis: collect the residue in (2), put in the distilled water of 6-8 times amount, be warming up to 50-60 DEG C, regulate pH5.5-7, add enzyme preparation, be hydrolyzed 2-3h；Add aqua calcis, regulate pH value to 9-10, be warming up to 75 DEG C, after keeping 40-60min, naturally cool to 50 DEG C, filter cleaner, obtain filtrate II-1；Collect residue, repeat above operation, obtain filtrate II-2；Merging filtrate I, II-1 and II-2.
(4) concentration: the filtrate after merging being put in vacuum decker, concentrating under reduced pressure, concentrated solution is the 70% of stock solution.
(5) alcohol analysis: be slowly added into 95% ethanol in concentrated solution, reach 70% to concentration of alcohol, standing sedimentation 8-12h, precipitating thing is astragalus polysaccharides, mechanical filter, then by 95% washing with alcohol 1-2 time.
(6) the astragalus polysaccharides precipitating thing after washing is carried out lyophilization, then pulverize, cross 100 mesh sieves, obtain astragalus polysaccharide extract.
2. a kind of astragalus polysaccharides extraction process as claimed in claim 1, it is characterized in that: described enzyme preparation is the mixture of cellulase and papain, for the 1.5%-3.0% of raw material astragalus weight, wherein cellulase 0%-1.5%, papain 0-1.5%；It is preferably the 2.2% of raw material astragalus weight, wherein cellulase 1.2% and papain 1.0%.
3. a kind of astragalus polysaccharides extraction process as claimed in claim 1, it is characterised in that: described astragalus polysaccharide extract total sugar content is more than 50.2%, and yield is more than 9.78%.
4. the astragalus polysaccharide extract as described in claim 1,3, it is characterised in that: as a kind of crude drug of enhancing human body immunity and sterilizing and anti-virus medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410828015.7A CN105777921A (en) | 2014-12-26 | 2014-12-26 | Astragalus polysaccharide extraction process and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410828015.7A CN105777921A (en) | 2014-12-26 | 2014-12-26 | Astragalus polysaccharide extraction process and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105777921A true CN105777921A (en) | 2016-07-20 |
Family
ID=56388638
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410828015.7A Pending CN105777921A (en) | 2014-12-26 | 2014-12-26 | Astragalus polysaccharide extraction process and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105777921A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109125275A (en) * | 2018-09-30 | 2019-01-04 | 河南黑马动物药业有限公司 | A kind of preparation method of astragalus root polysaccharide frozen dried powder |
| CN112791151A (en) * | 2021-02-20 | 2021-05-14 | 山东新稀宝股份有限公司 | Composition with function of improving sleep and application |
| CN113749981A (en) * | 2021-10-25 | 2021-12-07 | 广州煜明生物科技有限公司 | Application of astragalus polysaccharide in preparation of cosmetics with moisture absorption, moisture retention or relieving effects |
| CN114948792A (en) * | 2022-06-23 | 2022-08-30 | 伊深细胞生物技术(上海)有限公司 | Preparation method of wash-free smearing type stock solution for hair growth and hair loss prevention |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4886666A (en) * | 1987-01-27 | 1989-12-12 | Yaguang Liu | Pharmaceutical composition for inhibiting viruses and increasing immune function (PII) |
| CN1510143A (en) * | 2002-12-24 | 2004-07-07 | 中国农业大学 | Extraction of milk vetch polysaccharide by enzyme treatment |
| JP2006070217A (en) * | 2004-09-03 | 2006-03-16 | Kitasato Inst:The | Polysaccharide and biophylactic activation agent originated from aboveground part of astragalus mongholicus bunge |
| CN101934009A (en) * | 2010-08-03 | 2011-01-05 | 北京同仁堂（亳州）饮片有限责任公司 | Preparation method for processed arisaema tubers |
| CN103288974A (en) * | 2013-05-13 | 2013-09-11 | 茅兴娟 | Method for extracting astragalus polysaccharide |
-
2014
- 2014-12-26 CN CN201410828015.7A patent/CN105777921A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4886666A (en) * | 1987-01-27 | 1989-12-12 | Yaguang Liu | Pharmaceutical composition for inhibiting viruses and increasing immune function (PII) |
| CN1510143A (en) * | 2002-12-24 | 2004-07-07 | 中国农业大学 | Extraction of milk vetch polysaccharide by enzyme treatment |
| JP2006070217A (en) * | 2004-09-03 | 2006-03-16 | Kitasato Inst:The | Polysaccharide and biophylactic activation agent originated from aboveground part of astragalus mongholicus bunge |
| CN101934009A (en) * | 2010-08-03 | 2011-01-05 | 北京同仁堂（亳州）饮片有限责任公司 | Preparation method for processed arisaema tubers |
| CN103288974A (en) * | 2013-05-13 | 2013-09-11 | 茅兴娟 | Method for extracting astragalus polysaccharide |
Non-Patent Citations (2)
| Title |
|---|
| 国家中医药管理局专业技术资格考试专家委员会编: "《全国中医药专业技术资格考试大纲与细则 中药专业 中级》", 31 December 2011 * |
| 钱存忠主编: "《兽药速查手册》", 30 June 2014 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109125275A (en) * | 2018-09-30 | 2019-01-04 | 河南黑马动物药业有限公司 | A kind of preparation method of astragalus root polysaccharide frozen dried powder |
| CN112791151A (en) * | 2021-02-20 | 2021-05-14 | 山东新稀宝股份有限公司 | Composition with function of improving sleep and application |
| CN113749981A (en) * | 2021-10-25 | 2021-12-07 | 广州煜明生物科技有限公司 | Application of astragalus polysaccharide in preparation of cosmetics with moisture absorption, moisture retention or relieving effects |
| CN113749981B (en) * | 2021-10-25 | 2024-01-26 | 广州煜明生物科技有限公司 | Application of astragalus polysaccharide in preparing cosmetics with moisture absorption, moisturizing or relieving effects |
| CN114948792A (en) * | 2022-06-23 | 2022-08-30 | 伊深细胞生物技术(上海)有限公司 | Preparation method of wash-free smearing type stock solution for hair growth and hair loss prevention |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102816251B (en) | Preparation method of high-activity astragalus polysaccharide and pharmaceutical composition of high-activity astragalus polysaccharide | |
| CN102988827B (en) | Dendrobium officinale granula | |
| CN101698819B (en) | Method for preparing traditional Chinese medicine health-care liquor | |
| CN102988828B (en) | Preparation method of dendrobium officinale granula | |
| CN103768152B (en) | Flos Osmanthi Fragrantis phenethyl alcohol glycoside extract and its production and use | |
| CN102417546B (en) | Extraction method of rose crude polysaccharide | |
| CN104292352B (en) | A kind of Cortex Eucommiae fine powder, eucommia bark polycose and gutta-percha coproduction extraction separation method | |
| CN105777921A (en) | Astragalus polysaccharide extraction process and application thereof | |
| CN105504079A (en) | Process for producing astragalus polysaccharide by using ultrasonic technology | |
| CN110917291A (en) | Composition with function of improving immunity and preparation method and application thereof | |
| CN102408494B (en) | Grifola frondosa polysaccharide ZZK component and preparation method thereof | |
| CN105400845A (en) | Technology for producing radix astragali polysaccharide by utilization of microbial fermentation technology | |
| CN112535239B (en) | Compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock and preparation and application thereof | |
| CN103860616A (en) | Manyprickle Acathopanax Root extrat, and preparation method and application thereof | |
| CN102617893A (en) | Preparation and application of composition of panaxoside Rg3, ginseng polysaccharide and ganoderan | |
| CN107912771A (en) | A kind of stem of noble dendrobium composition with enhancing immune function and preparation method thereof | |
| CN101899120A (en) | Method for refining jujube polysaccharide | |
| CN108261418B (en) | Preparation and drying method of pachyman powder | |
| CN105399852A (en) | Technology for producing radix astragali polysaccharide by utilization of alkaline process extraction technology | |
| CN104031171A (en) | High molecular weight selfheal polysaccharide, and preparation method and application thereof in immune medicaments | |
| CN113694152A (en) | Method for obtaining coix seed extracting solution by high-stability enzymolysis method | |
| CN103054911A (en) | Crocodile antineoplastic preparation and production method thereof | |
| CN105440148A (en) | Process for producing astragalus polysaccharide by using water extraction technology | |
| CN101358222A (en) | Method for microwave auxiliarily extracting antiradiation polysaccharide using potentilla anserine as feedstock | |
| CN109528857A (en) | A kind of campanulaceae particle and preparation method thereof for treating respiratory disease caused by haze |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160720 |
|
| RJ01 | Rejection of invention patent application after publication |