DE19742775A1 - Microscopy mounting medium - Google Patents
Microscopy mounting mediumInfo
- Publication number
- DE19742775A1 DE19742775A1 DE1997142775 DE19742775A DE19742775A1 DE 19742775 A1 DE19742775 A1 DE 19742775A1 DE 1997142775 DE1997142775 DE 1997142775 DE 19742775 A DE19742775 A DE 19742775A DE 19742775 A1 DE19742775 A1 DE 19742775A1
- Authority
- DE
- Germany
- Prior art keywords
- additives
- parts
- microscopy
- mounting medium
- isopropanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000386 microscopy Methods 0.000 title claims description 3
- 239000012120 mounting media Substances 0.000 title 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 8
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000000654 additive Substances 0.000 claims abstract description 7
- 230000002380 cytological effect Effects 0.000 claims abstract description 4
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims abstract description 4
- 239000004205 dimethyl polysiloxane Substances 0.000 claims abstract description 4
- 239000000203 mixture Substances 0.000 claims abstract description 4
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims abstract description 4
- 229920002037 poly(vinyl butyral) polymer Polymers 0.000 claims abstract description 4
- 229920001296 polysiloxane Polymers 0.000 claims abstract description 4
- 239000011521 glass Substances 0.000 claims abstract description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 9
- 239000004721 Polyphenylene oxide Substances 0.000 claims description 3
- KXDHJXZQYSOELW-UHFFFAOYSA-N carbonic acid monoamide Natural products NC(O)=O KXDHJXZQYSOELW-UHFFFAOYSA-N 0.000 claims description 3
- 229920000570 polyether Polymers 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 229920005989 resin Polymers 0.000 claims description 3
- 239000011347 resin Substances 0.000 claims description 3
- KJAMZCVTJDTESW-UHFFFAOYSA-N tiracizine Chemical compound C1CC2=CC=CC=C2N(C(=O)CN(C)C)C2=CC(NC(=O)OCC)=CC=C21 KJAMZCVTJDTESW-UHFFFAOYSA-N 0.000 claims description 3
- 229920001577 copolymer Polymers 0.000 claims description 2
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 claims description 2
- 229920001807 Urea-formaldehyde Polymers 0.000 abstract 1
- 230000000996 additive effect Effects 0.000 abstract 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 abstract 1
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 6
- 238000001035 drying Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000008096 xylene Substances 0.000 description 2
- 206010019233 Headaches Diseases 0.000 description 1
- 101100345589 Mus musculus Mical1 gene Proteins 0.000 description 1
- 229920003015 aliphatic polyurethane dispersion Polymers 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
- G01N2001/364—Embedding or analogous mounting of samples using resins, epoxy
Abstract
Description
Die Erfindung betrifft ein flüssiges Einschlußmittel für zytologische Präparate.The invention relates to a liquid inclusion agent for cytological Preparations.
Es kann angewendet werden in der Zytologie, der Hystologie, und der Botanik bei der Fixierung von Untersuchungsobjekten, wie Pro ben und Schnitten auf Objektträgern aus Glas zur Mikroskopierung derselben.It can be used in cytology, hystology, and botany in the fixation of objects under investigation, such as Pro Practice and cuts on glass slides for microscopy the same.
Es ist bekannt, dazu verschiedene Klarlacke auf der Bindemittelbasis von Kunstharzen zu verwenden, die als Lösungsmittel im wesentli chen Toluol und Xylol enthalten. Diese Lösungsmittel sind physiolo gisch nicht unbedenklich. Sie haben kanzerogene Eigenschaften und weisen einen unangenehmen Geruch auf. Sie können auch Kopfschmerzen verursachen. Nachteilig ist weiterhin, bei Anwen dung derselben, deren relativ lange Trockenzeit und auch, daß die ausgehärteten Einschlußmittel nicht reversibel sind, das heißt, daß diese nicht wieder vom Objekt und vom Objektträger ablösbar sind, ohne das Objekt zu beschädigen. Bei der Entsorgung solcher Objektträger sind diese als Sondermüll zu behandeln, was zusätz liche Aufwendungen verursacht.It is known to use various clear coats based on the binder of synthetic resins to be used as a solvent in the main Chen toluene and xylene contain. These solvents are physiolo not harmless. They have carcinogenic properties and have an unpleasant smell. You can also Cause headache. Another disadvantage is with users the same, their relatively long drying time and also that the cured inclusion agents are not reversible, that is these cannot be detached from the object and the slide without damaging the object. When disposing of such Slides are to be treated as special waste, which is additional caused expenses.
Es wurde auch bereits eine aliphatische Polyurethandispersion mit Wasser verdünnbar zur Verwendung als flüssiges Einschlußmittel vorgeschlagen. Nachteilig ist auch dabei, daß ein solches Einschluß mittel ebenfalls nicht reversibel ist und daß sich die damit behandel ten bzw. die darin eingeschlossenen Objekte bei längerer Aufbe wahrungszeit verfärben, was zur Verfälschung der Untersuchungs ergebnisse führen kann.An aliphatic polyurethane dispersion has also been used Water thinnable for use as a liquid inclusion agent suggested. Another disadvantage is that such an inclusion medium is also not reversible and that they are treated with it objects or the objects enclosed in them in the event of prolonged exposure discolor, causing falsification of the investigation can lead to results.
Problem der Erfindung ist es, ein physiologisch unbedenkliches Ein schlußmittel für Objekte zur Fixierung auf Objektträgern zu schaffen welches bei kurzer Trockenzeit ein gutes Haftvermögen aufweist, eine dauerhafte Farberhaltung des Objektes gewährleistet und welches sich einfach, ohne das Erfordernis eines Deckglases am Objektträger verarbeiten läßt.The problem of the invention is a physiologically harmless one to create closing means for objects for fixation on slides which has good adhesion in a short drying time, ensures permanent color preservation of the object and which is easy to do without the need for a cover slip Can process slides.
Erfindungsgemäß wird ein Einschlußmittel geschaffen, das aus 15 bis 25% Polyvinylbutyral, 45 bis 55% Ethanol, 25 bis 30% Isopropanol und 1 bis 6% Additive besteht, wobei die Additive ein Gemisch aus etwa einem Teil Silikon, drei Teilen Polyäther modifiziertes Dimethyl polysiloxan Copolymer und dreißig Teilen Carbamedsäureharz sind.According to the invention, an inclusion means is created which consists of 15 to 25% polyvinyl butyral, 45 to 55% ethanol, 25 to 30% isopropanol and 1 to 6% additives, the additives being a mixture of about one part silicone, three parts polyether modified dimethyl polysiloxane copolymer and thirty parts of carbamic acid resin.
Das erfindungsgemäße Einschlußmittel ist physiologisch unbedenk lich und nicht geruchsbelästigend. Es weist ein gutes Haftungsver mögen sowie einen dauerhaften Farberhalt auf und läßt sich mittels Pinsel ohne die Verwendung von Deckgläsern für die Objektträger leicht verarbeiten. Das erfindungsgemäße Einschlußmittel ist rever sibel und läßt auch vom Objekt und vom Objektträger ablösen, womit eine Neueinfärbung oder eine Umfärbung des Objektes er möglicht wird. Die Trocknungszeit beträgt 15 bis 20 Minuten, gegen über einer Trocknungszeit von 20 bis 30 Minuten bei der Verwen dung bekannter Einschlußmittel. Die Entsorgung ist ohne besonde ren Aufwand möglich und die Verwendung desselben in zytolo gischen und anderen speziellen Laboratorien erfordert dort keine besondere Bevorratung von Toluol und/oder Xylol.The inclusion agent according to the invention is physiologically harmless Lich and not bad smell. It has a good liability record like as well as permanent color retention and can be Brushes without the use of cover glasses for the slides easy to process. The inclusion agent according to the invention is rever sibel and can also be detached from the object and the slide, with which he recolored or recolored the object is possible. The drying time is 15 to 20 minutes, against over a drying time of 20 to 30 minutes when used of known inclusion agents. The disposal is without special possible effort and the use of the same in cytolo nical and other special laboratories does not require any special storage of toluene and / or xylene.
Nachstehend wird die Erfindung an einem Ausführungsbeispiel näher erläutert.The invention is illustrated below using an exemplary embodiment explained in more detail.
Mit der zugehörigen Tabelle sind die Eigenschaften des erfindungs gemäßen Produktes mit einem Produkt nach dem Stand der Tech nik vergleichsweise dargestellt.With the associated table, the properties of the invention according product with a product according to the state of the art nik shown comparatively.
Das flüssige Einschlußmittel für zytologische Präparate enthält 20% Polyvinylbutyral, 50% Ethanol, 25% Isopropanol und 5% Additive, wobei die Additive ein Gemisch von einem Teil Silikon, drei Teilen Polyäther modifiziertes Dimethylpolysiloxan Copolymer und 30 Teilen Carbamedsäureharz besteht. The liquid inclusion agent for cytological preparations contains 20% Polyvinyl butyral, 50% ethanol, 25% isopropanol and 5% additives, the additives being a mixture of one part silicone, three parts Polyether modified dimethylpolysiloxane copolymer and 30 parts Carbamic acid resin exists.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE1997142775 DE19742775B4 (en) | 1997-09-27 | 1997-09-27 | entrapment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE1997142775 DE19742775B4 (en) | 1997-09-27 | 1997-09-27 | entrapment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE19742775A1 true DE19742775A1 (en) | 1999-04-01 |
| DE19742775B4 DE19742775B4 (en) | 2005-03-17 |
Family
ID=7843873
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE1997142775 Expired - Fee Related DE19742775B4 (en) | 1997-09-27 | 1997-09-27 | entrapment |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE19742775B4 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1055123A1 (en) * | 1998-02-10 | 2000-11-29 | Lee H. Angros | Analytic plate and method |
| US7731811B2 (en) | 2005-04-15 | 2010-06-08 | Angros Lee H | Analytic substrate coating apparatus and method |
| US8192994B2 (en) | 1998-02-10 | 2012-06-05 | Angros Lee H | Method of applying a biological specimen to an analytic plate |
| US11333587B2 (en) | 2006-08-25 | 2022-05-17 | Lee H. Angros | Analytic plates with markable portions and methods of use |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5938997B2 (en) * | 1979-05-15 | 1984-09-20 | 触媒化成工業株式会社 | coating composition |
| JPH0743275A (en) * | 1993-07-28 | 1995-02-14 | Sumitomo Metal Mining Co Ltd | Powder observation sample and production thereof |
| JP3071678B2 (en) * | 1995-08-31 | 2000-07-31 | 三洋化成工業株式会社 | Immune reaction buffer |
-
1997
- 1997-09-27 DE DE1997142775 patent/DE19742775B4/en not_active Expired - Fee Related
Cited By (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8192994B2 (en) | 1998-02-10 | 2012-06-05 | Angros Lee H | Method of applying a biological specimen to an analytic plate |
| EP1055123A4 (en) * | 1998-02-10 | 2004-12-01 | Lee H Angros | Analytic plate and method |
| US9176029B2 (en) | 1998-02-10 | 2015-11-03 | Lee H. Angros | Method of applying a biological specimen to an analytic plate |
| EP1055123A1 (en) * | 1998-02-10 | 2000-11-29 | Lee H. Angros | Analytic plate and method |
| US8450116B2 (en) | 1998-02-10 | 2013-05-28 | Lee H. Angros | Method of applying a biological specimen to an analytic plate |
| US8820378B2 (en) | 2005-04-15 | 2014-09-02 | Lee H. Angros | Analytic substrate coating apparatus and method |
| US9255863B2 (en) | 2005-04-15 | 2016-02-09 | Lee H. Angros | Analytic substrate coating apparatus and method |
| US8048472B2 (en) | 2005-04-15 | 2011-11-01 | Angros Lee H | Analytic substrate coating method |
| US8470109B2 (en) | 2005-04-15 | 2013-06-25 | Lee H. Angros | Analytic substrate coating method |
| US8470264B2 (en) | 2005-04-15 | 2013-06-25 | Lee H. Angros | Analytic substrate coating method |
| US8006638B2 (en) | 2005-04-15 | 2011-08-30 | Angros Lee H | Analytic substrate coating apparatus and method |
| US7731811B2 (en) | 2005-04-15 | 2010-06-08 | Angros Lee H | Analytic substrate coating apparatus and method |
| US8048245B2 (en) | 2005-04-15 | 2011-11-01 | Angros Lee H | Analytic substrate coating method |
| US9568401B2 (en) | 2005-04-15 | 2017-02-14 | Lee H. Angros | Analytic substrate coating apparatus and method |
| US9909961B2 (en) | 2005-04-15 | 2018-03-06 | Lee H. Angros | Analytic substrate coating apparatus and method |
| US10222306B2 (en) | 2005-04-15 | 2019-03-05 | Lee H. Angros | Analytic substrate coating apparatus and method |
| US10578524B2 (en) | 2005-04-15 | 2020-03-03 | Lee H. Angros | Analytic substrate coating apparatus and method |
| US10996148B2 (en) | 2005-04-15 | 2021-05-04 | Lee H. Angros | Analytic substrate coating apparatus and method |
| US11307121B2 (en) | 2005-04-15 | 2022-04-19 | Lee H. Angros | Analytic substrate coating apparatus and method |
| US11692915B2 (en) | 2005-04-15 | 2023-07-04 | Lee H. Angros | Analytic substrate coating apparatus and method |
| US11333587B2 (en) | 2006-08-25 | 2022-05-17 | Lee H. Angros | Analytic plates with markable portions and methods of use |
Also Published As
| Publication number | Publication date |
|---|---|
| DE19742775B4 (en) | 2005-03-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8122 | Nonbinding interest in granting licenses declared | ||
| 8110 | Request for examination paragraph 44 | ||
| 8127 | New person/name/address of the applicant |
Owner name: LADEMANN, CHRISTL, 02763 ZITTAU, DE Owner name: ZITTWITZ, WOLFHARD, 02763 ZITTAU, DE Owner name: REGEL, GABRIELE, 32547 BAD OEYNHAUSEN, DE |
|
| 8364 | No opposition during term of opposition | ||
| R084 | Declaration of willingness to license | ||
| R119 | Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee |