DE20207301U1 - Expression system for synthetic genes in microalgae - Google Patents

Expression system for synthetic genes in microalgae

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Publication number
DE20207301U1
DE20207301U1 DE20207301U DE20207301U DE20207301U1 DE 20207301 U1 DE20207301 U1 DE 20207301U1 DE 20207301 U DE20207301 U DE 20207301U DE 20207301 U DE20207301 U DE 20207301U DE 20207301 U1 DE20207301 U1 DE 20207301U1
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DE
Germany
Prior art keywords
nucleic acids
acids according
nucleic acid
elements
encodes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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DE20207301U
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German (de)
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UNIVERSITAET REGENSBURG, DE
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EICHLER STAHLBERG ALKE
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Application filed by EICHLER STAHLBERG ALKE filed Critical EICHLER STAHLBERG ALKE
Priority to DE20207301U priority Critical patent/DE20207301U1/en
Publication of DE20207301U1 publication Critical patent/DE20207301U1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Description

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßt The description text was not recorded electronically  

Der Beschreibungstext wurde nicht elektronisch erfaßtThe description text was not recorded electronically

Claims (13)

1. Nukleinsäurepolymere, die eine Transkriptionseinheit darstellen, wobei die Nukleinsäure mindestens aus den Elementen A, B und D besteht (Abb. 1), wobei A als Promoter fungiert, B die Exportsequenz kodiert und D das Strukturgen oder Genfragment darstellt, das für ein Protein kodiert, welches erfindungsgemäß synthetisiert und ausgeschleust werden soll.1.Nucleic acid polymers which represent a transcription unit, where the nucleic acid consists of at least the elements A, B and D ( FIG. 1), where A acts as a promoter, B codes the export sequence and D represents the structural gene or gene fragment which is essential for a protein encodes which should be synthesized and discharged according to the invention. 2. Teilsynthetische Nukleinsäurepolymere, die eine Transkriptionseinheit darstellen, wobei die Nukleinsäure mindestens aus den Elementen A, B und D besteht (Abb. 1), wobei A als Promoter fungiert, B die Exportsequenz kodiert und D das Strukturgen oder Genfragment darstellt.2. Partially synthetic nucleic acid polymers which represent a transcription unit, the nucleic acid consisting at least of the elements A, B and D ( FIG. 1), where A acts as a promoter, B encodes the export sequence and D represents the structural gene or gene fragment. 3. Nukleinsäuren nach Anspruch 1, wobei das Nukleinsäurepolymer zusätzlich eins oder mehrere der Elemente C, E und/oder F enthält (Abb. 1), wobei C ein N-terminales Epitop und E ein C-terminales Epitop kodieren, die zur Proteindetektion und/oder Proteinreinigung dienen, und F eine 3' untranslatierte Region (UTR) darstellt.3. Nucleic acids according to claim 1, wherein the nucleic acid polymer additionally contains one or more of the elements C, E and / or F ( FIG. 1), where C encodes an N-terminal epitope and E a C-terminal epitope, which are used for protein detection and / or serve protein purification, and F represents a 3 'untranslated region (UTR). 4. Nukleinsäuren nach Anspruch 1, wobei B die Exportsequenzen der Arylsulfatase = ARS oder des Chlamyopsin 3 = COP3 aus Chlamydomonas reinhardtii darstellen (Abb. 3).4. Nucleic acids according to claim 1, wherein B represent the export sequences of arylsulfatase = ARS or chlamyopsin 3 = COP3 from Chlamydomonas reinhardtii ( Fig. 3). 5. Nukleinsäuren nach Anspruch 1, wobei A den HSP-RBCS2-In1-Promotor nach Lit10 darstellt (Abb. 3).5. Nucleic acids according to claim 1, wherein A represents the HSP-RBCS2-In1 promoter according to Lit 10 ( Fig. 3). 6. Nukleinsäuren nach Anspruch 2, wobei 0 ein synthetisches Nukleinsäurepolymer darstellt, u. z. vorzugsweise den S-Tag [Novageil] und/oder eine Proteaseerkennungssequenz, vorzugsweise für Faktor Xa (Abb. 3).6. Nucleic acids according to claim 2, wherein 0 represents a synthetic nucleic acid polymer, preferably the S-Tag [Novageil] and / or a protease recognition sequence, preferably for factor Xa ( Fig. 3). 7. Nukleinsäuren nach Anspruch 5, wobei E ein synthetisches Nukleinsäurepolymer darstellt, das vorzugsweise den HSV-tag [Novagen] kodiert (Abb. 3).7. Nucleic acids according to claim 5, wherein E represents a synthetic nucleic acid polymer which preferably encodes the HSV tag [Novagen] ( Fig. 3). 8. Nukleinsäuren nach Anspruch 5, wobei F die 3' UTR des RBCS2-Gens aus Chlamydomonas reinhardtii darstellt (Abb. 3).8. Nucleic acids according to claim 5, wherein F represents the 3 'UTR of the RBCS2 gene from Chlamydomonas reinhardtii ( Fig. 3). 9. Nukleinsäuren nach Anspruch 1 bis 7, wobei einzelne Elemente A, B, C, E oder F mutiert sind, aber noch mindestens 80% Homologie zu den ursprünglichen unter 1 bis 8 beschriebenen Elementen aufweisen.9. Nucleic acids according to claim 1 to 7, wherein individual elements A, B, C, E or F mutate are, but still at least 80% homology to the original under 1 to 8 have described elements. 10. Vektoren, die Nukleinsäuren nach Anspruch 1 bis 9 enthalten.10. Vectors containing nucleic acids according to claim 1 to 9. 11. Mikroorganismen, die DNA oder Vektoren gemäß Anspruch 1 bis 11 enthalten.11. Microorganisms containing DNA or vectors according to claims 1 to 11. 12. Proteine, die von Nukleinsäuren gemäß Anspruch 1 bis 11 codiert werden. 12. Proteins which are encoded by nucleic acids according to claims 1 to 11.   13. Proteine, die von Mikroorganismen gemäß Anspruch 11 produziert und sezerniert werden.13. Proteins which are produced and secreted by microorganisms according to claim 11.
DE20207301U 2002-05-10 2002-05-10 Expression system for synthetic genes in microalgae Expired - Lifetime DE20207301U1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE20207301U DE20207301U1 (en) 2002-05-10 2002-05-10 Expression system for synthetic genes in microalgae

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE20207301U DE20207301U1 (en) 2002-05-10 2002-05-10 Expression system for synthetic genes in microalgae

Publications (1)

Publication Number Publication Date
DE20207301U1 true DE20207301U1 (en) 2002-12-19

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Country Link
DE (1) DE20207301U1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1481059A1 (en) * 2002-02-13 2004-12-01 XOMA Technology Ltd. Eukaryotic signal sequences for polypeptide expression and polypeptide display libraries

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1481059A1 (en) * 2002-02-13 2004-12-01 XOMA Technology Ltd. Eukaryotic signal sequences for polypeptide expression and polypeptide display libraries
US7396661B2 (en) 2002-02-13 2008-07-08 Xoma Technology Ltd. Eukaryotic signal sequences for polypeptide expression and polypeptide display libraries
US7972811B2 (en) 2002-02-13 2011-07-05 Xoma Technology Ltd. Eukaryotic signal sequences for polypeptide expression and polypeptide display libraries
US7977068B2 (en) 2002-02-13 2011-07-12 Xoma Technology Ltd. Eukaryotic signal sequences for polypeptide expression and polypeptide display libraries
US8476040B2 (en) 2002-02-13 2013-07-02 Xoma Technology Ltd. Eukaryotic signal sequences for polypeptide expression and polypeptide display libraries

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Date Code Title Description
R086 Non-binding declaration of licensing interest
R207 Utility model specification

Effective date: 20030130

R150 Term of protection extended to 6 years

Effective date: 20051123

R081 Change of applicant/patentee

Owner name: UNIVERSITAET REGENSBURG, DE

Free format text: FORMER OWNER: MARKUS FUHRMANN,ALKE EICHLER-STAHLBERG,PETER HEGEMANN, , DE

Effective date: 20060316

R157 Lapse of ip right after 6 years

Effective date: 20081202