DE3246071A1 - Method of genetic analysis and synthesis - Google Patents

Abstract

Using test products of natural and biosynthetic origin, the synthetic method aims at decoding the genetic code and divides the information of the genes into four internal corpus effects, one internal situs effect and one external situs effect in the gene chain. Artificial gene programmes are obtained in the polyrecombinator also by four-phase inoculation of the bacteria and shape-retaining transfer of complete mononucleotide series of four of different internal situs. They facilitate genetic analysis which takes place with computer and imaging screen assistance with matrix gene mechanics. In this connection, a new way to find species-specific restrictive enzymes used as scalpels for many types of genetic hereditary factors is also deduced from the new corpus and situs analysis. The corpus and situs effects yield a periodic table of living genes as partner of the periodic table of dead elements, and a register of species-specific enzyme scalpels is provided for the periodic gene table, each single one of which aims at a particular gene or its situs. The known function of DNA balance in the reception of interfering signals and defence effect termini is taken over and manipulated both for RNA and for DNA in order in this way to optimise communication.

Description

Method of genetic analysis

and Synthesis The invention described below relates to a method genetic analysis and synthesis. The analysis presupposes the synthesis and Vice versa, because the analysis serves to decipher the genetic code and that can can only be made possible experimentally with biosynthetic test products; and vice versa can systematically create genetic art programs only from knowledge of the gene code The decoding of the gene chain is used for biosynthetic pharmacy in humans and animals and the improvement of species in plants - in these innovations it is assumed that the single gene of the genetic brief is not as a "letter" of conventional writing, but as a matrix comparable to the picture lines of ancient Egyptian picture scripts is to be understood; with such a hieroglyphic matrix rather a whole word or a sentence of the gene chain information content the set consists of four organic bases that act as mononucleotides in the purine ADENIN (a), GUANINE (g) in the purine, CYTOSINE (c) in the pyrimidine and THYMIN (t) in the pyrimidine Their structural design is determined by the internal gene matrix, and there is also one external gene matrix intertwined with it.

According to the Watson-Crick model 1953, the gene in Cleave the above four fragments. They individually become the bacteria of a culture inoculated, multiply with them, and go in the recombiner device after multiplying to the acceptor equipped with the initial mononucleotide inoculation, similar to at chromosome transfer the discoverer Tatum et alii, überlum on it the recombinant to form in several recombination steps. How it comes about that the four gene corpses (a, c, g, t) thousands of different hereditary factors are present in a chromosome chain Question mark of genetic art The following analysis is intended to answer this question, and the analysis should open systematic biosynthesis. When the latter goes one from assigning a corpusoriontierte hereditary disposition to the gene / and consequently in the new biosynthetic processes, the invention consists in d a s s the Inventor and his synthesis one of physical, chemical and biological Data of the corpuses (a, u, g, t) derived corpus effeot together with one the order internal of these four mononucleotides and superimposed on the corpus effect Situseffeot in their sum contain gene-internal inheritance, this binary internal sum effeot However, an external situs effect is superimposed on the gene, whichever is the case in its determinant the classification of the DNA proposed as a gene in the gene chain qn a certain Classification place expresses, so that gene internal effect plus gene external effect in mutual Overlay on the one hand the genetic content of the gene, on the other hand in the multitude of genes integrated and, because of the external effect, intertwined the overall inheritance the chain also represents, with sequence change within the gene between (a, c, g, t) and not only changing the external placement of a gene in the chain change the matrix of the internal gender determinants, but rather the matrix of the whole chain.

This main inventive idea creates for the new systematic gene synthesis process new requirements. The analysis and the thought processes leading to the synthesis and Structures are interpreted in figures of the drawing Fig.1. shows the chemical-analytical Structure of one of the four organic bases involved in biosynthesis, known as Mononucleotides. It is of the type CYTOSIN (t). the Characters H, O, N, C are standard chemical symbols.

FIG. 2 shows a cross section of the DNA two-strand helix, with the helix axis perpendicular to the picture surface, namely Fig. 2a shows the molecular pairing (g + c) of one strand, and Fig. 2b. the molecular pair wig (a + t) of the other strand.

Fig. 3. shows the chemical symbols of Fig.2. used symbols.

Fig. 4. shows the connection of the four mononulcheotids (a, c, g, t) which were referred to here as organic bases with OB. The deoxyribose (sugar) is with Z and phosphoric acid with P;. represent the Greek letters the digits of the Kandewitz picture The pictures of figures 1., 2., 3., 4., are taken from the book Kaudewitz, Molecular and Microbe Genetics HT115-1973.

As modifications of Fig. 4. show Fig.5. a mononucleotide series of four of the No. 1. of Fig. 8, and their links, Fig. 6. a mononucleotide series of four of the No. 9. of Fig. 9, and their links.

These series can be selected as programmable examples from a total of 24 feasible rows No.1 to No.24 of Figures 8 to 11.

7 shows the mononucleotides involved in structuring the linkage of the gene nucleus from FIG. 4. goes. They are also shown in the following with letters designated.

Figures 8 through 11 show as groups A, B, D, E in a set of 4 boxes on row numbers 1 to 24 the 24 gene-internal variations of the mononucleotide sequence, which ground feasible in this selection and the 24 varieties in the internal corpus effect of the same; with each of these 24 corpus effeote the associated geninternal The situs effect is shifting and the result of the intertwining of these two internal effects resulting hereditary disposition of the genes superimposed as an internal sum from the external situs effeot is where the place occurs where the gene is in the chromosome chain is classified.

Fig. 12 shows a gene chain, bent three times for space reasons.

In it are the 24 rows of four from No.1. to No.24 of groups A, B, D, E arranged in such a way that the end of a previous row starts at the beginning of the following is linked.

The distribution of the four mononucleotides is optimal, nowhere in the The same letters follow one another in a gene chain.

Arrows indicate the sense of attachment. The 24 rows of four are the Figures 8 to 11 assigned.

Fig. 13 shows a 96-membered gene chain as it is, for example, hardly in the sense 4. becomes linkable Whether it delivers usable contents if the link successful is uncertain.

To this day, disX effect transmission and di impulse recording are based on the relevant organisms or from the periphery in the genes dollll eriferal metabolism attributed to UNA-ulld RNA cells. Without impulse pick-up and effect transfer would the gene chain cannot bring out any genetic make-up in the organism in which it was innate or artificially introduced. It is also conceivable that also the consistently structured gene chain in FIG. 12.

despite optimal distribution, besides effective hereditary factors also "deaf" Contains genes, i.e. those that contain no vital information. It is known that e.g.

Plasmids alone can transfer "deaf genes".

Fig. 14. shows one of the 24 rows of four No. 1. until 24.

of FIGS. 7 to 11. 96-membered gene chain formed.

Here, two identical letters periodically follow each other at intervals comes from the fact that when the 24 rows of four are linked together, the last link is one Row was not linked to the first of the following row as in Fig. 12, but the last link in a row is attached to the last of the following row The design is programmable and may provide usable genetic makeup in all genes, nevertheless periodically two equals lie next to each other.

Fig.15. shows the both for the analysis and deciphering of the genetic Code like computer indispensable for biosynthetic programming (41) species-specific Design with included memory and switches, the key mechanism (44), the control lines (73), the lines to external software (72); to the computer and the ones it contains Data storage, which is preferably tailored to the determinants of matrix gene mechanics is a data viewing screen (42) and an isometric work process with axes x, y, z drawing room screen coupled, which is labeled (43). In this Analysis equipment will be used to decipher the genetic code resulting from analyzes of the Test samples processed and stored data. The unit aims at a periodic system of living genes, as a parallel to the periodic system of the dead elements. As from the latter from "empty subjects" the incentive came to If new elements are found, the empty compartments of the former are supposed to be new biosynthetic ones Programs for Wiren. -Fig. 16. shows the well-known four-step recombiner. He serves on the one hand the synthesis of routine test products for analysis, on the other hand also new ones Biosytltllesen, whose program is taken from the The structure of this recombiner is known from earlier publications and consists of the suspension tank (55) with bottom (56), upper distributor (53), spring tube cover (51) with removable hollow Acceptor (52). The latter protrudes into the bore of the pipe (54) hanging on (53). In the gap between (52) and (54) the pumped down in this reaction space delivers Bacterial culture prepared the DNA transition from the nutrient solution to the surface d.s. Acceptors (52).

The boiler is mounted on the hollow foot (57). Therein as (58) a Hydraulic cylinder installed, which lifts the lifting frame (59) with the piston rod (60 () and can lower. At the top (59) is attached to Deokel (51) and the rod (60) can Lift the lid to remove the coated acceptor so that afterwards a new acceptor with attached initial mononucleotide coupled to the lid and this can be lowered again to the next cycle the suspension (a) from tank (66) or with the suspension (o) from (67) or

recombined with (g) from (68) or with (t) from (69), where (a, c, g, t) the four mononucleotide vaccinations of the bacteria in question. Between The steps are rinsed with tap water from (70) the boiler, the water over (71). pumped out again into the environmental protection tank. (61) is vent pipe, (62) Filling and emptying pipes (63,64) are circulation pipes; It is circulated with a pump (45). The box (65) contains all valves and other fittings and the box (46) the relays and contactors.

The recombiner is controlled with the program from (44).

Fig.17. In contrast to the mono-recombiner type of FIG. 16, FIG Poly recombinator type. With this innovation, there is an en block DNA junction at every step arranged from rows of four (a, c, g, t). For this purpose the bacteria of the tank simultaneously four mononucleotides (a, c, g, t) in a certain order of the Four rows of inoculated, all bacteria in a tank in the same order.

The bacteria reproduce this four-chain true to shape when cells divide thanks to the well-known principle of replication. This is how the four-mononucleotides go form scattered on the acceptor, although in the conventional framework also metabolism and mutation occur.

For example, if you put 24 tanks (74 to 97) in place, the bacteria from each tank the rows of four of the type of row No.1.

vaccinated up to No.24 of Figures 8 to 11, then you get 24 recombination holes a recombine according to the table in FIG. 19 .; while the monitor combiner of Fig. 16.

in 4 Sohritten a recombine according to the table in FIG. 18. generated.

Fig. 18. Step by step, within a cycle, (66) or from (67) or from (68) or from (69) recombined; in the individual sohritten only 1 mononucleotide each (a) or (c) or (g) or (t) recombined from bacteria, each of which carries the same one-piece inoculation in the same tanks.

The four steps can be iterated in several rotations, such as this table shows.

Fig. 19. As you can see, the 24 tanks (74) to (97) are each used once Four recombination has been used and it formed on the acceptor of the Polyrekombinators Kekombinate with iterating four-mononucleotide groups.

One after the other, the group is sedimented on croup from the 24 tanks The order results from the row numbers No. 1. to No..24. of Figures 8, until 11.

Recombinations of Tables 18 and 19 form the above as for the Analysis of indispensable named test products.

Their chemical, physical, biological data are used as matrices deposited in the memory and processed in the computer.

With gene matrix ~ mechanics you get the desired connections from the large number of tosts. The skeleton of the entrance is derived from this mentioned periodic system of genes, which starting point for the new biosynthesis shall be.

Carrying out the analysis With or without increased yield with MASSER = Molecularbiological Amplification by Shake-Stimulating the Effect of Recombination A variety of test products are generated. The StSSER yield enhancement is provided by the pulse device (49) is supplied, which is indicated by dashed lines in Fig. 16 because it is not necessarily is necessary. Ultrasound from 500 to 500,000 Hertz is used as a reinforcing impulse with a motor (47) or a friction or. Compressor device (48) in the sound shaper (49) and from there into the tube (50) and the hollow acceptor (52). The latter transmits the impulses to the suspension in the tubular reaction gap between (52) and (54) and generates high-frequency, high-impulse molecular motion in it, which reproduces on the bacteria as a stimulus.

What can be determined from the test pieces would be the following: 1. Outcrops via the formation of layers, the equal or uneven distribution biologically identical and biologically different genes.

2. Information about the chain formation 3. Information about metabolism and mutations.

4. Information about periodicities of the test data Internal corpus and situs effects.

6. Information about external site effects of the classification of genes in gene chains.

7. Digestions of the above types in the case of test products made from natural products generated by gene surgery and gene recombination are 8. Information about the important Ask why restrictive enzymes past thousands of genes in a chain are their target of the chain gate.

9. Inclusions that follow from point 8. and that can lead to all possible genetic surgical incision sites of a gene chain are assigned to the incision site-specific ones Identify enzymes as scalpels.

10. From the result of point 9. Information about how to additionally a restrictive species-specific gene surgery with a Variety of enzyme scalpels can plan, which in addition to the synthesis of new gene chains can be formed from natural chains.

Dialysis and synthesis are carried out in the computer using matrix gene mechanics. Treating their matrices in detail is neither sensible nor possible. Bas is also not necessary. Because mathematical elements are logic, i.e. rules of the game, and they as Auxiliary rubbing is not a matter for P patent claims do not have to be defeated. by the way, the matrix is too Already known in the description of the gene, if it is also processed differently here.

Logic and analogy in analysis and synthesis.

With logical elements of the description, the living nucleus can be described as Carriers of the gene chain-coated as an analog of the atomic or molecular structure This is most evident in the outward effects of structural changes: If in a gene within the row of four there is a corpus changes its situs, changes the gene matrix or its influence in the genetic make-up, analogous to the effeot of the quantum emission of a molecular amplifier which occurs when In its atomic shell structure an electron changes the situs, i.e. the level four corpuses (a to t) of the gene represent the core components of the atom, the four Situses represent the shell structure of atomic nucleus electrons, which is what the gene represents Atom. The gene chain is the molecule of chemistry made up of a multitude of different atoms structure, similar to the gene chain. The external site effect of the gene in the gene chain is analogous to the effect of molecular agglomeration of several different ones Atoms. The chemical properties of the molecule change with the properties its structural components, analogous to the change in biological properties the gene chain.

Implementation of the synthesis analogies are in the course of the technical Progress has almost always been the starting point for innovation. What here in Connection of (corpus + situs) -Interneffeoten of the gene and its external situs effect Innovations should arise in the chain of several genes: 1. Monosynthesis in the sense of Figures 5., 6., 18., 18.

2. The polysynthesis in the sense of Figures 5.6.17.19.

3. In search of new restriction enzymes an expansion in the pairing of gene surgery with gene combination.

While in points 1 and 2, simpler gene reconstructions are preferred n from the retort "are pending, it is at point 2.

consequent continuations of the current enzymatic practice of targeted gene surgery and subsequent recombination to the most diverse bariations; whereby natural products are almost always assumed.

When finding new restrictive enzymes, one can assume that their 'aiming ability "prefers that which still aims at occult information in the genes to be excluded from a chain, the "interneffeot" of the gene is, i.

the sum of four corpus effeotes and their situs effeotes in the row of four. Whether the trace which, starting from the gene, leads to the enzyme as a "beacon", is in the effeotes cell metabolism, or whether it is a type of biological emission (Ray) is, stays open. It can also be a digital counting track, though looking for the enzyme on the chain and for example a 1 or a 0 seeks to insert itself at 0, and there to separate the chain.

When looking for this enzyme for a particular target gene, the "Agreement" play an important role in the electronic process. In the older one Shooting range practice of the sports and military shooters was called "agreement" by the shooter The number of rings is reported after the shot, which the shooter has with after the shot the rifle calmly continued to aim to find out what he was getting at with the Sohuss.

For this search for enzymes and the notification of the agreement could Cumulative gene chains of biosynthetic design of the type shown in Figure 13 may be useful.

In the analysis and in the analytical search for new goals aiming at Enzymes are provided by the isometric screen made up of linear gene and gene chain matrices running lines, from their shape, lead, mutual position, especially from their Important statements follow intersections: All lines and also the data on the data screen (42) can be triggered and triggered items are saved spontaneously.

Bacteria serve as carriers for the enzyme samples in nutrient solution Plasmids which are oingo-planted to them.

As a complement to the periodic table of genes is in the course of discovery Each gene is assigned a species-specific enzyme and all together are used as an enzyme register added to the periodic gene system; where the genetic periodic system and its enzyme register then replaces the conventional gene mapping.

The emerging realization, namely that apparently the metabolic balance The DNA components are responsible as receivers of interfering signals and special Reject commands and they insofar the communication between hereditary disposition, The disturbance mechanism and defense mechanism are supplied in the new corpus and situs-oriented Gene analysis and gene synthesis taken over with the extension that also the metabolic functions the le - as the carrier of the l) nt in this communication, presumably as a signal leader; he, partnetlmen.

In this respect, the optimizing manipulation is particularly important in synthesis processes of the DNA and IZNA S t ofi variants in the production of pharmaceutical products ex Vitro included. With in vivo therapy, the DNA metabolism is manipulated ex vitro, that of the RNA is adapted because it is preprogrammed.

Litteratur Kaudewirtz "Molecular and microbe genetics Heidelberger Pocket b. llT 115 1973.

L e r s e i t e

Claims (16)

Claims 1. A method of genetic analysis and synthesis, thereby denotes that as the starting point for the gene, the hereditary disposition caused by the substance and they as the internal inheritance effect sum of the gene as the corpus effect of the four organic bases (a, o, g, t) and as a situs effect of the internal order of (a, o, g, t) is assigned whereby the situses superimpose the corpuses in their effects, and as external effect this internal effect sum is superimposed by the effect of the external Classification of the gene in the ohromosomal gene chain, with those from these two types of situs and the corpus effect formed effect sum for the current gene structure and gene classification, its breeding information is as long as the internal and the external situs does not change, but this genetic disposition changes as soon as one of the both types of situs sic changed, and whereby from the sum of the genetic inheritance in the Chain integrates a chain inheritance that is only valid as long as all internal ones and external sites remain unchanged, but also the integrated ones when there are changes Genetic chain pea sum is changed. 2. Method according to claim 1, characterized in that it is at The analysis is used to decipher the genetic code by using natural gene chains or artificial gene chains of known hereditary structures with gene surgery and recombination changed, the previous and subsequent physical, chemical, biological Data is stored and such tests in a wide variety of computer and screen aids The effects and functions of the gene chain changes are compared and evaluated to obtain and evaluate with matrix genetic mechanics. 3. METHOD according to claims 1. and R. However, marked by comparing known gene chains with restructured ones according to FIGS. 7. to 11., according to which the new structures in the four corpuses (a, c, g, t) of the genes in the sense Yet Claim 3. of Fig.4. of the Kaudewitz model in the 24 possible internal sequences of the rows of four No.1 to No.24 are linked, so that 24 different situses there are options for which no letter in the row of four occurs twice, which is the case with Thousands of genes in a chain, many thousands of hereditary factors from these four sites can be expected if one makes external situations variant. 4. The method according to claims 1 to 3. characterized in that Test products with gene chains in 24 differently ordered gene corpses, screen-supported compared and derived conclusions for the influence of the internal gene sites will. 5. The method according to claims 1 to 4, characterized in that In a poly recombiner, the bacteria in the suspension are each a complete set of four the Genoorpusse (a, c, g, t) is inoculated, this set of the individual bacterium Cell division is doubled and decoupled to the acceptor, creating a convenient Process for reproduction and sedimentation that is true to shape because it is supported by replication in the manufacture of test products with different internal situs effects the genes are created. 6. The method according to claim 5, according to Fig.17. and 19. characterized in that On the poly recombiner 24 tanks with nutrient solution contain bacteria, one per tank the 24 four-goal turning of Fig. 8. carry up to 11th as a four-phase vaccination, multiply and <in selective 24 steps transferred to the acceptor0 7. Procedure according to Claims 1 to 6, characterized in that the determined from fading Data regarding the internal genital influence of the four components (a, c, g, t) and the external genetic influence processed into a periodic system of genes and this system as the basis for the selection of targeted genetic biosynthetic Programs and syntheses is used. 8. The method according to claims 1 to 7, characterized in that with biosynthetic products as the target of planned programming, all possible enzymes are tested for their restriction properties and compared to the results of the analysis, which shows the connection between corpus factor, internal Situs factor and external situs factor provided, a key to the question of restrictiveii Suitability of enzymes and an explanation of the restrictive targeting ability of the enzymes is found, according to which new enzymes assigned to their targets obtained from analysis and thus new genetic engineering is obtained. 9. The method of synthesis according to claim 8, characterized in that that then preferably from natural gene chains of special choice with the matching ones found enzymes genes excluded and inserted in the recombiner other genes to get gene chain restructuring. 10. The method according to claims 8 and 9 and according to claim 7. characterized by the fact that the poriodic system of the living Genes from the systematic finding of assigned genes and finding all possible gene targets Enzymes as a scalpel register are assigned to the register of enzymes distributed pleriodically will. 11. The method according to claims 1 to 10 for analysis and synthesis characterized in that the processing sequences are linearly programmed gene and Gene chain matrices can be triggered on the data screen (42) together with the teletext and on the isometrically calibrated screen (43) as curved and straight spatial lines shown from their mutual position and in particular from shapes and intersections triggerable conclusions about the searched connections, with all data storable and triggered data are stored. 12. Ancestors according to claims 8 to 9, thereby gekeniizoichnet, that in the testing of enzymes as scalpels of gene surgery for untorodious Aims bacteria to serve as their implantation vehicle in nutrient solution, preferably too those which contain plasmids, or wherein the enzyme is a plasmid and the plasmid then it is applied to the bacterium. 13. The method according to claims 1 .bis12. Characterized in that be comparison of the system of dead elements to the periodic system of living elements Genes the structure of the dead atom corresponds to the structure of the living single gene, where in living genes the four organic bases (a, c, g, t) are the core particles Neutrons and protons of the atomic nucleus of dead matter, and the four situses of the nucleus particles (a, c, g, t) of the gene are equivalent to the electrons of the atomic sohale, according to which from a dead Atomic structure of the physical molecular amplifier is emitted as a quantum Signal that an idlectron has changed its level position during the Gene of the internal situs change or the outer situs via the RNA metabolism or delivers a signal of the disturbance to the organism on another path, and these analogics also look for enzymes with different targets are used, and thus new synthesis or. Surgical procedures are opened, wherein Analogy and analysis are synthetic or surgical instruments. 14. The method according to claims 1 to 13 when evaluating the data of situs superimpositions of the corpus effects characterized in that in the computer the superimposed internal and external situs effects of the 4 corpus effects of the GenkerFe from matrix according to the principles of phase demodulation with disoriminator formula or the amplitude demodulation is de-ciphered with the principle of the rectifier, or that it is based on the principle of interference patterns, as with optical overlays is decoded. 15. The method according to claims 1 to 14, characterized in that that with the new one tailored to corpus and situs effects of the genes and gene chains Make the biosynthesis so the current knowledge is adopted that namely the metabolic balance of the DNA components for communication as a recipient of interference pulses and transmitters of defense and similar effects in the application that hereditary disposition is responsible, and consequently when it is based on corpus and situs effects new synthesis processes the metabolic effect of both DNA and the one the RNA carrying them is manipulated in an optimizing manner accordingly. 16. The method according to claim 15, characterized in that bci applications of the in vitro process, this metabolism affects DNA and preselected for RNA When used in vivo, it is used at the same time as the preparation produced in vitro the genetic makeup is manipulated, both on the DNA members as well as on the they carry RNA which can no longer be selected and optimized in vivo.