DE3476455D1 - Polymer catalyst transducers and use thereof in test kits for analytical methods - Google Patents

Polymer catalyst transducers and use thereof in test kits for analytical methods

Info

Publication number
DE3476455D1
DE3476455D1 DE8484112709T DE3476455T DE3476455D1 DE 3476455 D1 DE3476455 D1 DE 3476455D1 DE 8484112709 T DE8484112709 T DE 8484112709T DE 3476455 T DE3476455 T DE 3476455T DE 3476455 D1 DE3476455 D1 DE 3476455D1
Authority
DE
Germany
Prior art keywords
analytical methods
test kits
polymer catalyst
transducers
polymer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE8484112709T
Other languages
English (en)
Inventor
Kung Wei-Jen Hu
Paul O Vogelhut
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Miles Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Inc filed Critical Miles Inc
Application granted granted Critical
Publication of DE3476455D1 publication Critical patent/DE3476455D1/de
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
DE8484112709T 1983-11-02 1984-10-22 Polymer catalyst transducers and use thereof in test kits for analytical methods Expired DE3476455D1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/548,152 US4560534A (en) 1983-11-02 1983-11-02 Polymer catalyst transducers

Publications (1)

Publication Number Publication Date
DE3476455D1 true DE3476455D1 (en) 1989-03-02

Family

ID=24187632

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8484112709T Expired DE3476455D1 (en) 1983-11-02 1984-10-22 Polymer catalyst transducers and use thereof in test kits for analytical methods

Country Status (11)

Country Link
US (1) US4560534A (de)
EP (1) EP0140322B1 (de)
JP (1) JPS60113142A (de)
AT (1) ATE40472T1 (de)
AU (1) AU569332B2 (de)
CA (1) CA1222932A (de)
DE (1) DE3476455D1 (de)
DK (1) DK160781C (de)
ES (1) ES8603649A1 (de)
IL (1) IL73206A (de)
NO (1) NO163584C (de)

Families Citing this family (108)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4855243A (en) * 1983-09-26 1989-08-08 H.S.G. Venture (A Joint Venture Partnership) Neurological and biological molecular electro-optical devices and methods
US4721601A (en) * 1984-11-23 1988-01-26 Massachusetts Institute Of Technology Molecule-based microelectronic devices
US4895705A (en) * 1984-11-23 1990-01-23 Massachusetts Institute Of Technology Molecule-based microelectronic devices
US4717673A (en) * 1984-11-23 1988-01-05 Massachusetts Institute Of Technology Microelectrochemical devices
US4791374A (en) * 1986-12-12 1988-12-13 The Lubrizol Corporation Acid sensor
US5023133A (en) * 1986-12-12 1991-06-11 The Lubrizol Corporation Acid sensor
DE3788356T2 (de) * 1986-12-15 1994-06-23 British Tech Group Usa Monomere phthalocyanin-reagenzien.
US5494793A (en) * 1986-12-15 1996-02-27 British Technology Group Usa Inc. Monomeric phthalocyanine reagents
US5346670A (en) * 1986-12-24 1994-09-13 British Technology Group U.S.A. Inc. Phthalocyanine and tetrabenztriazaporphyrin reagents
US5151224A (en) * 1988-05-05 1992-09-29 Osaka Gas Company, Ltd. Tetrasulfonated metal phthalocyanine doped electrically conducting electrochromic poly(dithiophene) polymers
US5108576A (en) * 1988-06-13 1992-04-28 Ohmicron Corporation Pyroelectric thermometric device
US5156810A (en) * 1989-06-15 1992-10-20 Biocircuits Corporation Biosensors employing electrical, optical and mechanical signals
US5491097A (en) * 1989-06-15 1996-02-13 Biocircuits Corporation Analyte detection with multilayered bioelectronic conductivity sensors
US5818636A (en) * 1990-02-26 1998-10-06 Molecular Displays, Inc. Complementary surface confined polmer electrochromic materials, systems, and methods of fabrication therefor
US5457564A (en) * 1990-02-26 1995-10-10 Molecular Displays, Inc. Complementary surface confined polymer electrochromic materials, systems, and methods of fabrication therefor
US5202261A (en) * 1990-07-19 1993-04-13 Miles Inc. Conductive sensors and their use in diagnostic assays
US5250439A (en) * 1990-07-19 1993-10-05 Miles Inc. Use of conductive sensors in diagnostic assays
US5593852A (en) * 1993-12-02 1997-01-14 Heller; Adam Subcutaneous glucose electrode
JPH04278450A (ja) 1991-03-04 1992-10-05 Adam Heller バイオセンサー及び分析物を分析する方法
US5580527A (en) * 1992-05-18 1996-12-03 Moltech Corporation Polymeric luminophores for sensing of oxygen
US6660484B2 (en) * 1992-11-13 2003-12-09 Regents Of The University Of California Colorimetric glycopolythiophene biosensors
US5547555A (en) * 1993-02-22 1996-08-20 Ohmicron Technology, Inc. Electrochemical sensor cartridge
SE510733C2 (sv) 1995-01-03 1999-06-21 Chemel Ab Kemisk sensor baserad på utbytbar igenkänningskomponent samt användning därav
US5795453A (en) * 1996-01-23 1998-08-18 Gilmartin; Markas A. T. Electrodes and metallo isoindole ringed compounds
US5830341A (en) * 1996-01-23 1998-11-03 Gilmartin; Markas A. T. Electrodes and metallo isoindole ringed compounds
DE69809391T2 (de) 1997-02-06 2003-07-10 Therasense Inc Kleinvolumiger sensor zur in-vitro bestimmung
DE19737714C2 (de) * 1997-08-29 2002-10-02 Volkswagen Ag Ölalterungssensor
US6103033A (en) 1998-03-04 2000-08-15 Therasense, Inc. Process for producing an electrochemical biosensor
US6134461A (en) 1998-03-04 2000-10-17 E. Heller & Company Electrochemical analyte
US8688188B2 (en) 1998-04-30 2014-04-01 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US8974386B2 (en) 1998-04-30 2015-03-10 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US9066695B2 (en) 1998-04-30 2015-06-30 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US8465425B2 (en) 1998-04-30 2013-06-18 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US6175752B1 (en) 1998-04-30 2001-01-16 Therasense, Inc. Analyte monitoring device and methods of use
US8346337B2 (en) 1998-04-30 2013-01-01 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US6949816B2 (en) 2003-04-21 2005-09-27 Motorola, Inc. Semiconductor component having first surface area for electrically coupling to a semiconductor chip and second surface area for electrically coupling to a substrate, and method of manufacturing same
US8480580B2 (en) 1998-04-30 2013-07-09 Abbott Diabetes Care Inc. Analyte monitoring device and methods of use
US6251260B1 (en) 1998-08-24 2001-06-26 Therasense, Inc. Potentiometric sensors for analytic determination
US6338790B1 (en) 1998-10-08 2002-01-15 Therasense, Inc. Small volume in vitro analyte sensor with diffusible or non-leachable redox mediator
US6591125B1 (en) 2000-06-27 2003-07-08 Therasense, Inc. Small volume in vitro analyte sensor with diffusible or non-leachable redox mediator
JP4801301B2 (ja) 1999-06-18 2011-10-26 アボット ダイアベティス ケア インコーポレイテッド 物質移動が制限された生体内分析物センサー
US6616819B1 (en) 1999-11-04 2003-09-09 Therasense, Inc. Small volume in vitro analyte sensor and methods
EP1344219A2 (de) 2000-12-11 2003-09-17 Branimir Simic-Glavaski Molekulare elektrooptische schalter- oder speichervorrichtung und verfahren zu deren herstellung
US6560471B1 (en) 2001-01-02 2003-05-06 Therasense, Inc. Analyte monitoring device and methods of use
US7041468B2 (en) 2001-04-02 2006-05-09 Therasense, Inc. Blood glucose tracking apparatus and methods
US7381184B2 (en) 2002-11-05 2008-06-03 Abbott Diabetes Care Inc. Sensor inserter assembly
US8771183B2 (en) 2004-02-17 2014-07-08 Abbott Diabetes Care Inc. Method and system for providing data communication in continuous glucose monitoring and management system
US7811231B2 (en) 2002-12-31 2010-10-12 Abbott Diabetes Care Inc. Continuous glucose monitoring system and methods of use
US20040223912A1 (en) * 2003-05-07 2004-11-11 Montalto Michael Christopher Compositions and methods for non-invasive imaging of soluble beta-amyloid
EP1631561B1 (de) 2003-05-07 2010-08-18 General Electric Company Zusammensetzungen und verfahren zur in vivo abbildung von loeslichen beta-amyloid
US8066639B2 (en) 2003-06-10 2011-11-29 Abbott Diabetes Care Inc. Glucose measuring device for use in personal area network
USD914881S1 (en) 2003-11-05 2021-03-30 Abbott Diabetes Care Inc. Analyte sensor electronic mount
US8571624B2 (en) 2004-12-29 2013-10-29 Abbott Diabetes Care Inc. Method and apparatus for mounting a data transmission device in a communication system
US10226207B2 (en) 2004-12-29 2019-03-12 Abbott Diabetes Care Inc. Sensor inserter having introducer
US8333714B2 (en) 2006-09-10 2012-12-18 Abbott Diabetes Care Inc. Method and system for providing an integrated analyte sensor insertion device and data processing unit
US9788771B2 (en) 2006-10-23 2017-10-17 Abbott Diabetes Care Inc. Variable speed sensor insertion devices and methods of use
US9572534B2 (en) 2010-06-29 2017-02-21 Abbott Diabetes Care Inc. Devices, systems and methods for on-skin or on-body mounting of medical devices
US7731657B2 (en) 2005-08-30 2010-06-08 Abbott Diabetes Care Inc. Analyte sensor introducer and methods of use
US8512243B2 (en) 2005-09-30 2013-08-20 Abbott Diabetes Care Inc. Integrated introducer and transmitter assembly and methods of use
US7883464B2 (en) 2005-09-30 2011-02-08 Abbott Diabetes Care Inc. Integrated transmitter unit and sensor introducer mechanism and methods of use
US9743862B2 (en) 2011-03-31 2017-08-29 Abbott Diabetes Care Inc. Systems and methods for transcutaneously implanting medical devices
US20090105569A1 (en) 2006-04-28 2009-04-23 Abbott Diabetes Care, Inc. Introducer Assembly and Methods of Use
US9398882B2 (en) 2005-09-30 2016-07-26 Abbott Diabetes Care Inc. Method and apparatus for providing analyte sensor and data processing device
US7697967B2 (en) 2005-12-28 2010-04-13 Abbott Diabetes Care Inc. Method and apparatus for providing analyte sensor insertion
US9259175B2 (en) 2006-10-23 2016-02-16 Abbott Diabetes Care, Inc. Flexible patch for fluid delivery and monitoring body analytes
US8112240B2 (en) 2005-04-29 2012-02-07 Abbott Diabetes Care Inc. Method and apparatus for providing leak detection in data monitoring and management systems
US9521968B2 (en) 2005-09-30 2016-12-20 Abbott Diabetes Care Inc. Analyte sensor retention mechanism and methods of use
US7766829B2 (en) 2005-11-04 2010-08-03 Abbott Diabetes Care Inc. Method and system for providing basal profile modification in analyte monitoring and management systems
US11298058B2 (en) 2005-12-28 2022-04-12 Abbott Diabetes Care Inc. Method and apparatus for providing analyte sensor insertion
EP1968432A4 (de) 2005-12-28 2009-10-21 Abbott Diabetes Care Inc Einführung eines medizinischen gerätes
US7885698B2 (en) 2006-02-28 2011-02-08 Abbott Diabetes Care Inc. Method and system for providing continuous calibration of implantable analyte sensors
US7620438B2 (en) 2006-03-31 2009-11-17 Abbott Diabetes Care Inc. Method and system for powering an electronic device
US8226891B2 (en) 2006-03-31 2012-07-24 Abbott Diabetes Care Inc. Analyte monitoring devices and methods therefor
WO2007143225A2 (en) 2006-06-07 2007-12-13 Abbott Diabetes Care, Inc. Analyte monitoring system and method
GB0611736D0 (en) * 2006-06-14 2006-07-26 Johnson Matthey Plc Ion-conducting membrane
US8930203B2 (en) 2007-02-18 2015-01-06 Abbott Diabetes Care Inc. Multi-function analyte test device and methods therefor
US8732188B2 (en) 2007-02-18 2014-05-20 Abbott Diabetes Care Inc. Method and system for providing contextual based medication dosage determination
US8123686B2 (en) 2007-03-01 2012-02-28 Abbott Diabetes Care Inc. Method and apparatus for providing rolling data in communication systems
US8461985B2 (en) 2007-05-08 2013-06-11 Abbott Diabetes Care Inc. Analyte monitoring system and methods
US8665091B2 (en) 2007-05-08 2014-03-04 Abbott Diabetes Care Inc. Method and device for determining elapsed sensor life
US7928850B2 (en) 2007-05-08 2011-04-19 Abbott Diabetes Care Inc. Analyte monitoring system and methods
US8456301B2 (en) 2007-05-08 2013-06-04 Abbott Diabetes Care Inc. Analyte monitoring system and methods
WO2008150917A1 (en) 2007-05-31 2008-12-11 Abbott Diabetes Care, Inc. Insertion devices and methods
US8103456B2 (en) 2009-01-29 2012-01-24 Abbott Diabetes Care Inc. Method and device for early signal attenuation detection using blood glucose measurements
US9402544B2 (en) 2009-02-03 2016-08-02 Abbott Diabetes Care Inc. Analyte sensor and apparatus for insertion of the sensor
US20100213057A1 (en) 2009-02-26 2010-08-26 Benjamin Feldman Self-Powered Analyte Sensor
US9226701B2 (en) 2009-04-28 2016-01-05 Abbott Diabetes Care Inc. Error detection in critical repeating data in a wireless sensor system
US9184490B2 (en) 2009-05-29 2015-11-10 Abbott Diabetes Care Inc. Medical device antenna systems having external antenna configurations
US9314195B2 (en) 2009-08-31 2016-04-19 Abbott Diabetes Care Inc. Analyte signal processing device and methods
WO2011026148A1 (en) 2009-08-31 2011-03-03 Abbott Diabetes Care Inc. Analyte monitoring system and methods for managing power and noise
JP5431079B2 (ja) * 2009-09-09 2014-03-05 メタウォーター株式会社 新規化学発光試薬および新規化学発光法ならびにそれを用いた被検出物質の測定方法およびそれに使用するキット
WO2011032630A1 (de) * 2009-09-18 2011-03-24 Merck Patent Gmbh Polymerisierbare verbindungen und flüssigkristallmedien
WO2011041469A1 (en) 2009-09-29 2011-04-07 Abbott Diabetes Care Inc. Method and apparatus for providing notification function in analyte monitoring systems
WO2011041531A1 (en) 2009-09-30 2011-04-07 Abbott Diabetes Care Inc. Interconnect for on-body analyte monitoring device
USD924406S1 (en) 2010-02-01 2021-07-06 Abbott Diabetes Care Inc. Analyte sensor inserter
ES2881798T3 (es) 2010-03-24 2021-11-30 Abbott Diabetes Care Inc Insertadores de dispositivos médicos y procedimientos de inserción y uso de dispositivos médicos
US11064921B2 (en) 2010-06-29 2021-07-20 Abbott Diabetes Care Inc. Devices, systems and methods for on-skin or on-body mounting of medical devices
GB201107039D0 (en) 2011-04-26 2011-06-08 Syngenta Ltd Formulation component
US9980669B2 (en) 2011-11-07 2018-05-29 Abbott Diabetes Care Inc. Analyte monitoring device and methods
FI3300658T3 (fi) 2011-12-11 2024-03-01 Abbott Diabetes Care Inc Analyyttianturimenetelmiä
US9968306B2 (en) 2012-09-17 2018-05-15 Abbott Diabetes Care Inc. Methods and apparatuses for providing adverse condition notification with enhanced wireless communication range in analyte monitoring systems
US10259688B1 (en) * 2013-11-13 2019-04-16 Mumser, Inc. V-sling with hook positioning adapter
KR20170005457A (ko) * 2014-05-08 2017-01-13 주식회사 다이셀 유기 헤테로 고분자 및 그의 제조 방법
WO2016183493A1 (en) 2015-05-14 2016-11-17 Abbott Diabetes Care Inc. Compact medical device inserters and related systems and methods
US10213139B2 (en) 2015-05-14 2019-02-26 Abbott Diabetes Care Inc. Systems, devices, and methods for assembling an applicator and sensor control device
US11401426B1 (en) * 2015-05-22 2022-08-02 University Of South Florida Composite conducting polymer films
CN110461217B (zh) 2017-01-23 2022-09-16 雅培糖尿病护理公司 用于分析物传感器插入的系统、装置和方法
US11369973B2 (en) * 2017-11-14 2022-06-28 Eco Tec Mineria Corp. Method and device for milling and separation of solids and granular materials including metal containing materials as well as phytogenic materials with high level of silicon in a controlled airflow

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL102893C (de) * 1959-03-27
US3008879A (en) * 1960-03-18 1961-11-14 Miles Lab Diagnostic composition for the quantitative determination of glucose
US3421982A (en) * 1965-05-14 1969-01-14 Lab Equipment Corp Enzymatic analysis
US3574072A (en) * 1968-04-03 1971-04-06 Universal Oil Prod Co Polymerization of heterocyclic compounds
BE758864A (fr) * 1969-11-13 1971-04-16 Miles Lab Instrument de mesure de conductivite differentielle
US4334880A (en) * 1980-10-20 1982-06-15 Malmros Mark K Analytical device having semiconductive polyacetylene element associated with analyte-binding substance
US4722905A (en) * 1981-12-01 1988-02-02 National Research Development Corporation Semiconductors
DE3217883A1 (de) * 1982-05-12 1983-11-17 Siemens AG, 1000 Berlin und 8000 München Gassensor
DE3223545A1 (de) * 1982-06-24 1983-12-29 Basf Ag, 6700 Ludwigshafen Copolymere von pyrrolen, verfahren zu ihrer herstellung sowie ihre verwendung
US4413151A (en) * 1982-07-14 1983-11-01 Exxon Research & Engineering Co. Process for hydroxylating olefins using a supported osmium catalyst
ZA8420B (en) * 1983-01-24 1985-02-27 Lubrizol Enterprises Inc Electronically conducting polypyrrole and co-polymers of pyrrole,compositions containing them,methods for making them,and electro-chemical cells using them

Also Published As

Publication number Publication date
JPH0346064B2 (de) 1991-07-15
AU3419484A (en) 1985-05-09
DK520884A (da) 1985-05-03
NO163584C (no) 1990-06-20
IL73206A0 (en) 1985-01-31
CA1222932A (en) 1987-06-16
NO163584B (no) 1990-03-12
NO844143L (no) 1985-05-03
ES537227A0 (es) 1985-12-16
EP0140322B1 (de) 1989-01-25
EP0140322A2 (de) 1985-05-08
DK160781B (da) 1991-04-15
DK520884D0 (da) 1984-11-01
DK160781C (da) 1991-09-30
ATE40472T1 (de) 1989-02-15
JPS60113142A (ja) 1985-06-19
EP0140322A3 (en) 1986-07-16
ES8603649A1 (es) 1985-12-16
US4560534A (en) 1985-12-24
AU569332B2 (en) 1988-01-28
IL73206A (en) 1988-11-30

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