DE3687889D1 - Apparat fuer zellfusion. - Google Patents

Apparat fuer zellfusion.

Info

Publication number
DE3687889D1
DE3687889D1 DE8686117156T DE3687889T DE3687889D1 DE 3687889 D1 DE3687889 D1 DE 3687889D1 DE 8686117156 T DE8686117156 T DE 8686117156T DE 3687889 T DE3687889 T DE 3687889T DE 3687889 D1 DE3687889 D1 DE 3687889D1
Authority
DE
Germany
Prior art keywords
cell fusion
fusion
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE8686117156T
Other languages
English (en)
Other versions
DE3687889T2 (de
Inventor
Takanori Mochizuki
Mamoru Koga
Shinitiro Takayama
Kenzo Toda
Yoshiyuki Togawa
Katsuyuki Imai
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shimadzu Corp
Original Assignee
Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP19118585U external-priority patent/JPH0131037Y2/ja
Priority claimed from JP60280566A external-priority patent/JPH0657140B2/ja
Priority claimed from JP19193685U external-priority patent/JPH0324077Y2/ja
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Publication of DE3687889D1 publication Critical patent/DE3687889D1/de
Application granted granted Critical
Publication of DE3687889T2 publication Critical patent/DE3687889T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
DE8686117156T 1985-12-11 1986-12-09 Apparat fuer zellfusion. Expired - Lifetime DE3687889T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP19118585U JPH0131037Y2 (de) 1985-12-11 1985-12-11
JP60280566A JPH0657140B2 (ja) 1985-12-13 1985-12-13 細胞融合装置
JP19193685U JPH0324077Y2 (de) 1985-12-13 1985-12-13

Publications (2)

Publication Number Publication Date
DE3687889D1 true DE3687889D1 (de) 1993-04-15
DE3687889T2 DE3687889T2 (de) 1993-06-24

Family

ID=27326450

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8686117156T Expired - Lifetime DE3687889T2 (de) 1985-12-11 1986-12-09 Apparat fuer zellfusion.

Country Status (5)

Country Link
US (1) US4804450A (de)
EP (1) EP0225636B1 (de)
KR (1) KR890003947B1 (de)
CN (1) CN1021460C (de)
DE (1) DE3687889T2 (de)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3522610A1 (de) * 1985-06-12 1986-12-18 Kernforschungsanlage Jülich GmbH, 5170 Jülich Kammer fuer die behandlung von zellen im elektrischen feld
US4923814A (en) * 1986-05-09 1990-05-08 Electropore, Inc. High speed, high power apparatus for vesicle prealignment, poration, loading and fusion in uniform electric fields and method therefor
GB8807271D0 (en) * 1988-03-26 1988-04-27 Preece A W Cell fusion apparatus
US6001617A (en) * 1989-06-07 1999-12-14 Queen's University At Kingston Electroporation device and method of use
US5134070A (en) * 1990-06-04 1992-07-28 Casnig Dael R Method and device for cell cultivation on electrodes
CA2019758C (en) * 1990-06-25 2001-09-04 Kevin L. Firth Improved electroporation device and method
US5173158A (en) * 1991-07-22 1992-12-22 Schmukler Robert E Apparatus and methods for electroporation and electrofusion
DE69433933T2 (de) * 1993-03-23 2005-07-21 CBR Laboratories, Inc., Boston Verfahren und vorrichtung zur einkapselung biologisch aktiver substanzen in zellen
CN1058291C (zh) * 1993-03-26 2000-11-08 中国科学院等离子体物理研究所 低能离子束细胞修饰技术和装置
IL108775A (en) * 1994-02-25 2003-09-17 Univ Ramot Method for efficient incorporation of molecules into cells
US6773669B1 (en) 1995-03-10 2004-08-10 Maxcyte, Inc. Flow electroporation chamber and method
US6074605A (en) * 1995-03-10 2000-06-13 Entremed, Inc. Flow electroporation chamber and method
US5720921A (en) * 1995-03-10 1998-02-24 Entremed, Inc. Flow electroporation chamber and method
GB9517780D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US7029916B2 (en) * 2001-02-21 2006-04-18 Maxcyte, Inc. Apparatus and method for flow electroporation of biological samples
EP1456345B1 (de) 2001-08-22 2016-07-20 Maxcyte, Inc. Vorrichtung und verfahren zur elektroporation biologischer proben
WO2003026599A1 (en) * 2001-09-26 2003-04-03 The Procter & Gamble Company Personal cleansing compositions comprising silicone resin-containing adhesives
WO2004031353A2 (en) * 2002-09-30 2004-04-15 Maxcyte, Inc. Apparatus and method for streaming electroporation
WO2005066342A1 (en) * 2003-12-01 2005-07-21 Walters Richard E Non-uniform electric field chamber for cell fusion
CN101426929B (zh) * 2004-05-12 2011-06-08 麦克赛特股份有限公司 与可调流式电穿孔室相关的方法和装置
EP2270130B1 (de) 2005-06-13 2020-04-29 Tosoh Corporation Zellfusionsvorrichtung und Verfahren zur Zellfusion unter Verwendung derselben
KR100695272B1 (ko) * 2006-01-10 2007-03-14 단국대학교 산학협력단 이종 식물 세포융합장치
ITTO20060273A1 (it) * 2006-04-12 2007-10-13 Silicon Biosystem S P A Metodi ed apparati per la selezione e/o il processamento di particellle, in particolare per la lisi selettiva e/o ottimizzata di cellule
CN102533547B (zh) * 2011-12-15 2013-11-13 北京航空航天大学 一种剪切力-均匀电场联合刺激的细胞培养装置
CN102559492B (zh) * 2011-12-15 2013-08-14 北京航空航天大学 一种剪切力-电刺激平行方向联合加载的细胞培养装置
CA2881685C (en) 2012-08-14 2023-12-05 10X Genomics, Inc. Microcapsule compositions and methods
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9388465B2 (en) * 2013-02-08 2016-07-12 10X Genomics, Inc. Polynucleotide barcode generation
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014127510A1 (en) * 2013-02-20 2014-08-28 Jian Chen Methods and devices for electroporation
EP4053292A1 (de) 2014-06-26 2022-09-07 10X Genomics, Inc. Verfahren zur analyse von nukleinsäuren aus einzelzellen oder zellpopulationen
WO2017047098A1 (en) 2015-09-18 2017-03-23 Okinawa Institute Of Science And Technology School Corporation 3d polymeric insert to apply uniform electric field in circular cultureware

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE213360C (de) *
US4578167A (en) * 1982-09-28 1986-03-25 Biofusion, Inc. Cell fusion
US4441972A (en) * 1983-04-08 1984-04-10 D.E.P. Systems, Inc. Apparatus for electrofusion of biological particles
DE3317415A1 (de) * 1983-05-13 1984-11-15 Kernforschungsanlage Jülich GmbH, 5170 Jülich Kammer zur behandlung von zellen im elektrischen feld
DE3321239C2 (de) * 1983-06-11 1985-05-09 Kernforschungsanlage Jülich GmbH, 5170 Jülich Kammer zur Behandlung von Zellen im elektrischen Feld
DE3327690C2 (de) * 1983-08-01 1986-04-10 Kernforschungsanlage Jülich GmbH, 5170 Jülich Verfahren zur Fusion von Zellen
US4613083A (en) * 1984-06-21 1986-09-23 Nordson Corporation Adjustable powder spray gun
US4561961A (en) * 1984-07-27 1985-12-31 Biotronics Cooled microscope slide and electrode apparatus for use in live cell fusion system
US4578168A (en) * 1984-07-27 1986-03-25 Biotronics Apparatus for fusing live cells with electric fields
DE3501865A1 (de) * 1985-01-22 1986-07-24 GCA Corp., Bedford, Mass. Verfahren und vorrichtung zur elektrofusion von zellen
DE3522610A1 (de) * 1985-06-12 1986-12-18 Kernforschungsanlage Jülich GmbH, 5170 Jülich Kammer fuer die behandlung von zellen im elektrischen feld

Also Published As

Publication number Publication date
EP0225636A2 (de) 1987-06-16
CN1021460C (zh) 1993-06-30
KR890003947B1 (ko) 1989-10-13
KR870006182A (ko) 1987-07-09
US4804450A (en) 1989-02-14
EP0225636B1 (de) 1993-03-03
EP0225636A3 (en) 1988-09-07
DE3687889T2 (de) 1993-06-24
CN86108459A (zh) 1987-07-29

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8320 Willingness to grant licences declared (paragraph 23)