DE3851910D1 - Abtastsystem zur Bestimmung der Fluoreszenz. - Google Patents

Abtastsystem zur Bestimmung der Fluoreszenz.

Info

Publication number
DE3851910D1
DE3851910D1 DE3851910T DE3851910T DE3851910D1 DE 3851910 D1 DE3851910 D1 DE 3851910D1 DE 3851910 T DE3851910 T DE 3851910T DE 3851910 T DE3851910 T DE 3851910T DE 3851910 D1 DE3851910 D1 DE 3851910D1
Authority
DE
Germany
Prior art keywords
scanning system
determining fluorescence
fluorescence
determining
scanning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE3851910T
Other languages
English (en)
Other versions
DE3851910T2 (de
Inventor
Charles William Robertson
Rudy Johan Dam
James Merrill Prober
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PerkinElmer Health Sciences Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Application granted granted Critical
Publication of DE3851910D1 publication Critical patent/DE3851910D1/de
Publication of DE3851910T2 publication Critical patent/DE3851910T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J1/00—Photometry, e.g. photographic exposure meter
    • G01J1/02—Details
    • G01J1/04—Optical or mechanical part supplementary adjustable parts
    • G01J1/06—Restricting the angle of incident light
    • G01J2001/062—Restricting the angle of incident light by fibre-optic packed bundle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/12—Generating the spectrum; Monochromators
    • G01J2003/1226—Interference filters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
DE3851910T 1987-06-12 1988-06-01 Abtastsystem zur Bestimmung der Fluoreszenz. Expired - Lifetime DE3851910T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/060,874 US4833332A (en) 1987-06-12 1987-06-12 Scanning fluorescent detection system

Publications (2)

Publication Number Publication Date
DE3851910D1 true DE3851910D1 (de) 1994-12-01
DE3851910T2 DE3851910T2 (de) 1995-05-24

Family

ID=22032284

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3851910T Expired - Lifetime DE3851910T2 (de) 1987-06-12 1988-06-01 Abtastsystem zur Bestimmung der Fluoreszenz.

Country Status (7)

Country Link
US (1) US4833332A (de)
EP (1) EP0294996B1 (de)
JP (1) JPS63317770A (de)
AU (1) AU601048B2 (de)
CA (1) CA1299772C (de)
DE (1) DE3851910T2 (de)
IL (1) IL86695A (de)

Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6004446A (en) * 1984-03-29 1999-12-21 Li-Cor, Inc. DNA Sequencing
US4729947A (en) * 1984-03-29 1988-03-08 The Board Of Regents Of The University Of Nebraska DNA sequencing
US6086737A (en) * 1984-03-29 2000-07-11 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes and suitable labels therefor
US5207880A (en) * 1984-03-29 1993-05-04 The Board Of Regents Of The University Of Nebraska DNA sequencing
US6207421B1 (en) 1984-03-29 2001-03-27 Li-Cor, Inc. DNA sequencing and DNA terminators
US5863403A (en) * 1984-03-29 1999-01-26 The Board Of Regents Of The University Of Nebraska Digital DNA typing
US5360523A (en) * 1984-03-29 1994-11-01 Li-Cor, Inc. DNA sequencing
US5571388A (en) * 1984-03-29 1996-11-05 Li-Cor, Inc. Sequencing near infrared and infrared fluorescense labeled DNA for detecting using laser diodes and suitable labels thereof
US5135717A (en) * 1986-12-24 1992-08-04 British Technology Group Usa Inc. Tetrabenztriazaporphyrin reagents and kits containing the same
US5346670A (en) * 1986-12-24 1994-09-13 British Technology Group U.S.A. Inc. Phthalocyanine and tetrabenztriazaporphyrin reagents
US4919512A (en) * 1988-10-17 1990-04-24 Mcdonnell Douglas Corporation Wavelength shift switch and sensing system
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
JPH083481B2 (ja) * 1989-06-07 1996-01-17 日立ソフトウェアエンジニアリング株式会社 蛍光式電気泳動パターン読み取り装置
FR2649487B1 (fr) * 1989-06-21 1991-09-20 Europhor Sa Capillaire d'electrophorese a dispositif optique incorpore
US5108179A (en) * 1989-08-09 1992-04-28 Myers Stephen A System and method for determining changes in fluorescence of stained nucleic acid in electrophoretically separated bands
IT1234466B (it) * 1989-09-20 1992-05-18 Consiglio Nazionale Ricerche Lidar a fluorescenza differenziale e relativo metodo di rilevamento
US5091652A (en) * 1990-01-12 1992-02-25 The Regents Of The University Of California Laser excited confocal microscope fluorescence scanner and method
US5274240A (en) * 1990-01-12 1993-12-28 The Regents Of The University Of California Capillary array confocal fluorescence scanner and method
JP2524243B2 (ja) * 1990-04-12 1996-08-14 日立ソフトウェアエンジニアリング株式会社 蛍光式電気泳動パタ―ン読み取り装置
JP2814408B2 (ja) * 1990-05-22 1998-10-22 日立ソフトウェアエンジニアリング 株式会社 蛍光パターン読み取り装置および蛍光パターン読み取り方法
US5119316A (en) * 1990-06-29 1992-06-02 E. I. Du Pont De Nemours And Company Method for determining dna sequences
JP2814409B2 (ja) * 1990-11-30 1998-10-22 日立ソフトウェアエンジニアリング 株式会社 多色泳動パターン読み取り装置
US5162654A (en) * 1991-02-01 1992-11-10 Wisconsin Alumni Research Foundation Detection apparatus for electrophoretic gels
AU3816993A (en) * 1992-03-19 1993-10-21 Regents Of The University Of California, The Multiple tag labeling method for DNA sequencing
US5294799A (en) * 1993-02-01 1994-03-15 Aslund Nils R D Apparatus for quantitative imaging of multiple fluorophores
US5424841A (en) * 1993-05-28 1995-06-13 Molecular Dynamics Apparatus for measuring spatial distribution of fluorescence on a substrate
US6035914A (en) * 1993-10-22 2000-03-14 Martin Marietta Energy Systems Inc. Counterfeit-resistant materials and a method and apparatus for authenticating materials
WO1995019359A1 (en) * 1994-01-12 1995-07-20 Massachusetts Institute Of Technology Process for making xanthene or cubane based compounds, and protease inhibitors
JP3060001B2 (ja) * 1995-05-12 2000-07-04 日立ソフトウエアエンジニアリング株式会社 蛍光式電気泳動パターン読み取り装置
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system
JP3012794B2 (ja) * 1995-10-02 2000-02-28 日立ソフトウエアエンジニアリング株式会社 スキャナ装置
US5699157A (en) * 1996-07-16 1997-12-16 Caliper Technologies Corp. Fourier detection of species migrating in a microchannel
US5871628A (en) * 1996-08-22 1999-02-16 The University Of Texas System Automatic sequencer/genotyper having extended spectral response
AU4920297A (en) 1996-10-25 1998-05-15 Applied Imaging, Inc. Multifluor-fluorescence in situ hybridization (m-fish) imaging techniques using multiple multiband filters with image registration
US5880473A (en) * 1997-07-28 1999-03-09 Applied Imaging, Inc. Multifluor-fluorescence in-situ hybridization (M-FISH) imaging techniques using multiple multiband filters with image registration
US20030135122A1 (en) * 1997-12-12 2003-07-17 Spectrx, Inc. Multi-modal optical tissue diagnostic system
US6055451A (en) * 1997-12-12 2000-04-25 Spectrx, Inc. Apparatus and method for determining tissue characteristics
JP2949220B2 (ja) * 1998-02-12 1999-09-13 工業技術院長 極微小顕微鏡分光装置
US6027628A (en) * 1998-02-23 2000-02-22 Yamamura; Hidetaka Gel cassette for electrophoresis
DE19816487A1 (de) * 1998-04-14 1999-10-21 Bodenseewerk Perkin Elmer Co Vorrichtung zum Nachweis eines Fluoreszenzfarbstoffs
US6078390A (en) * 1998-05-04 2000-06-20 General Scanning, Inc. Scanning system and method of operation for automatically setting detection sensitivity
EP1112022A4 (de) * 1998-09-11 2004-08-04 Spectrx Inc Multi-modale optische gewebediagnosevorrichtung
US6036021A (en) * 1999-02-17 2000-03-14 C.C. Imex Package for electrophoresis gel
US6403947B1 (en) 1999-03-18 2002-06-11 Cambridge Research & Instrumentation Inc. High-efficiency multiple probe imaging system
US20040147843A1 (en) * 1999-11-05 2004-07-29 Shabbir Bambot System and method for determining tissue characteristics
US6696022B1 (en) * 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6462816B1 (en) * 2000-07-21 2002-10-08 Symyx Technologies, Inc. Parallel capillary electrophoresis system having signal averaging and noise cancellation
JP3690271B2 (ja) * 2000-11-29 2005-08-31 株式会社島津製作所 核酸の塩基配列決定のためのマトリックス値を得る方法
JP2002277326A (ja) * 2001-03-19 2002-09-25 Nireco Corp 分光測光装置
US6844563B2 (en) * 2001-05-22 2005-01-18 Matsushita Electric Industrial Co., Ltd. Fluorescence detecting device with integrated circuit and photodiode, and detection method
CA2453390A1 (en) * 2001-07-25 2003-02-06 Applera Corporation Time-delay integration in electrophoretic detection systems
US7262017B2 (en) 2001-09-14 2007-08-28 Torrey Pines Institute For Molecular Studies Diagnostic markers for ischemia
US7383134B2 (en) * 2002-01-15 2008-06-03 Piper James R Method and/or system for analyzing biological samples using a computer system
CA2477702A1 (en) * 2002-03-05 2003-09-18 Caliper Life Sciences, Inc. Mixed mode microfluidic systems abstract of the disclosure
US20050143933A1 (en) * 2002-04-23 2005-06-30 James Minor Analyzing and correcting biological assay data using a signal allocation model
US6999173B2 (en) * 2003-09-25 2006-02-14 Ffa Sciences Llc Method and apparatus for ratio fluorometry
AU2005227312B2 (en) * 2004-03-22 2011-04-07 Ffa Sciences, Llp Development and use of fluorescent probes of unbound analytes
US7351538B2 (en) * 2004-08-23 2008-04-01 U.S. Genomics Systems and methods for detecting and analyzing polymers
US20060257938A1 (en) * 2005-05-10 2006-11-16 Kleinfeld Alan M Method of screening for drugs that block ligand binding to a lipid binding protein
US7388665B2 (en) * 2005-05-20 2008-06-17 Tir Technology Lp Multicolour chromaticity sensor
EP2044403A1 (de) * 2006-07-18 2009-04-08 TIR Technology LP Verfahren und vorrichtung zur bestimmung von intensitäten und spitzenwellenlängen von licht
US7381972B1 (en) * 2006-07-24 2008-06-03 Science Applications International Corporation System and method for light fluorescence detection
US9164109B2 (en) 2006-10-27 2015-10-20 Alan Kleinfeld Use of probes for unbound metabolites
US8999636B2 (en) * 2007-01-08 2015-04-07 Toxic Report Llc Reaction chamber
US20100294665A1 (en) * 2007-07-12 2010-11-25 Richard Allen Method and system for transferring and/or concentrating a sample
DE102008011013B4 (de) * 2008-02-25 2014-11-13 Mevitec Gmbh Verfahren und Einrichtung zur komplexen Stoffwechselanalyse
US8361716B2 (en) * 2008-10-03 2013-01-29 Pathogenetix, Inc. Focusing chamber
EP2391883B1 (de) 2009-01-30 2018-03-07 Micronics, Inc. Tragbares hochleistungsfluoreszenznachweissystem
KR101851117B1 (ko) 2010-01-29 2018-04-23 마이크로닉스 인코포레이티드. 샘플-투-앤서 마이크로유체 카트리지
DE102012002086A1 (de) * 2012-02-06 2013-08-08 Carl Zeiss Meditec Ag Verfahren zum Untersuchen von biologischem Gewebe und Vorrichtungen zum Untersuchen und Behandeln des Gewebes
US9028776B2 (en) 2012-04-18 2015-05-12 Toxic Report Llc Device for stretching a polymer in a fluid sample
US8685708B2 (en) 2012-04-18 2014-04-01 Pathogenetix, Inc. Device for preparing a sample
WO2014100725A1 (en) 2012-12-21 2014-06-26 Micronics, Inc. Portable fluorescence detection system and microassay cartridge
EP2935908B1 (de) 2012-12-21 2019-08-14 PerkinElmer Health Sciences, Inc. Fluidkreisläufe und zugehörige herstellungsverfahren
WO2014100743A2 (en) 2012-12-21 2014-06-26 Micronics, Inc. Low elasticity films for microfluidic use
CA2911303C (en) 2013-05-07 2021-02-16 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
WO2014182847A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Device for preparation and analysis of nucleic acids
WO2014182844A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3588257A (en) * 1968-10-14 1971-06-28 Us Navy Flame photometric method and apparatus for quantitatively analyzing material specimens
US3764512A (en) * 1972-05-02 1973-10-09 Singer Co Laser scanning electrophoresis instrument and system
US4152723A (en) * 1977-12-19 1979-05-01 Sperry Rand Corporation Method of inspecting circuit boards and apparatus therefor
US4162405A (en) * 1978-05-23 1979-07-24 Britton Chance Flying spot fluoro-meter for oxidized flavoprotein and reduced pyridine nucleotide
US4451149A (en) * 1980-07-28 1984-05-29 Noeller Hans Guenter Polarization fluoroimmunoassay apparatus
US4421860A (en) * 1980-10-07 1983-12-20 The Regents Of The University Of California Homogeneous fluoroimmunoassay involving autocorrelation processing of optically sensed signals
AT390840B (de) * 1983-06-08 1990-07-10 Avl Verbrennungskraft Messtech Verfahren zur fluorimetrischen bestimmung der konzentration von in einer substanz enthaltenen stoffen und anordnung zur durchfuehrung dieses verfahrens
FR2556726B1 (fr) * 1983-12-20 1987-02-20 California Inst Of Techn Compositions a base d'oligonucleotides monocatenaires et procede pour leur preparation
GB2155176B (en) * 1984-01-16 1988-05-11 California Inst Of Techn Sequencing dna
US4729947A (en) * 1984-03-29 1988-03-08 The Board Of Regents Of The University Of Nebraska DNA sequencing
US4651011A (en) * 1985-06-03 1987-03-17 At&T Technologies, Inc. Non-destructive method for determining the extent of cure of a polymer
CA1340806C (en) * 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing

Also Published As

Publication number Publication date
EP0294996A3 (de) 1991-04-10
EP0294996A2 (de) 1988-12-14
IL86695A0 (en) 1988-11-30
CA1299772C (en) 1992-04-28
DE3851910T2 (de) 1995-05-24
IL86695A (en) 1992-01-15
JPS63317770A (ja) 1988-12-26
US4833332A (en) 1989-05-23
EP0294996B1 (de) 1994-10-26
AU1762088A (en) 1988-12-15
AU601048B2 (en) 1990-08-30

Similar Documents

Publication Publication Date Title
DE3851910T2 (de) Abtastsystem zur Bestimmung der Fluoreszenz.
DE3774488D1 (de) Verfahren zur routen-festlegung.
DE3768088D1 (de) Verfahren zur bestimmung der perfusion.
DE3772121D1 (de) Abtastendoskop zur chirurgischen verwendung.
DE69016951T2 (de) System zur Bestimmung der Tonerfüllhöhe.
DE69023577T2 (de) Ultraschallsystem zur Bestimmung des Profils von Festkörpern.
DE3789073D1 (de) System zur maschinellen Übersetzung.
DE3786279D1 (de) Verfahren zur selektivbestimmung von mikrobiellen nukleotiden.
DE3668060D1 (de) System zur fuellstandsmessung.
DE3750847D1 (de) Einrichtung zur Gefriertrocknung.
DE3781307T2 (de) Signalverarbeitungsmethode zur bestimmung des grundaufbaus von nukleinsaeuren.
DE3775388D1 (de) System zur aufgestaenderte fussboeden.
NO880982D0 (no) Avbildningssystemer.
DE3577000D1 (de) Pruefstelle zur bestimmung der adhaesion.
DE3780938T2 (de) Reagens zur bestimmung von chloridionen.
DE3789914T2 (de) Vorrichtung zur Bestimmung der Prioritätsordnung.
DE3854639T2 (de) Verfahren zur Bestimmung des gewünschten Bildsignalbereichs.
DE3887528T2 (de) Verfahren zur bestimmung des sauerstoffgehaltes.
DE3677708D1 (de) Vorrichtung zur bestimmung der nordrichtung.
DE3887753T2 (de) System zur bestimmung der cyanursäure in wasser.
DE3786490T2 (de) Optisches System zur Bilderzeugung.
DE68906167D1 (de) Zusammensetzung zur erkennung von paradontalen krankheiten.
DE3850087T2 (de) System zur Anzeige von Objekten.
DE3780201D1 (de) Verfahren zur bestimmung der cholinesteraseaktivitaet.
DE3586511D1 (de) Diagnostisches reagenz zur bestimmung von harnstickstoff.

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: NEN LIFE SCIENCE PRODUCTS, INC.(N.D.GES.DES STAATE