DE3851910T2 - Abtastsystem zur Bestimmung der Fluoreszenz. - Google Patents

Abtastsystem zur Bestimmung der Fluoreszenz.

Info

Publication number
DE3851910T2
DE3851910T2 DE3851910T DE3851910T DE3851910T2 DE 3851910 T2 DE3851910 T2 DE 3851910T2 DE 3851910 T DE3851910 T DE 3851910T DE 3851910 T DE3851910 T DE 3851910T DE 3851910 T2 DE3851910 T2 DE 3851910T2
Authority
DE
Germany
Prior art keywords
scanning system
determining fluorescence
fluorescence
determining
scanning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE3851910T
Other languages
English (en)
Other versions
DE3851910D1 (de
Inventor
Charles William Robertson
Rudy Johan Dam
James Merrill Prober
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PerkinElmer Health Sciences Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of DE3851910D1 publication Critical patent/DE3851910D1/de
Application granted granted Critical
Publication of DE3851910T2 publication Critical patent/DE3851910T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J1/00—Photometry, e.g. photographic exposure meter
    • G01J1/02—Details
    • G01J1/04—Optical or mechanical part supplementary adjustable parts
    • G01J1/06—Restricting the angle of incident light
    • G01J2001/062—Restricting the angle of incident light by fibre-optic packed bundle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/12—Generating the spectrum; Monochromators
    • G01J2003/1226—Interference filters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
DE3851910T 1987-06-12 1988-06-01 Abtastsystem zur Bestimmung der Fluoreszenz. Expired - Lifetime DE3851910T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/060,874 US4833332A (en) 1987-06-12 1987-06-12 Scanning fluorescent detection system

Publications (2)

Publication Number Publication Date
DE3851910D1 DE3851910D1 (de) 1994-12-01
DE3851910T2 true DE3851910T2 (de) 1995-05-24

Family

ID=22032284

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3851910T Expired - Lifetime DE3851910T2 (de) 1987-06-12 1988-06-01 Abtastsystem zur Bestimmung der Fluoreszenz.

Country Status (7)

Country Link
US (1) US4833332A (de)
EP (1) EP0294996B1 (de)
JP (1) JPS63317770A (de)
AU (1) AU601048B2 (de)
CA (1) CA1299772C (de)
DE (1) DE3851910T2 (de)
IL (1) IL86695A (de)

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Also Published As

Publication number Publication date
DE3851910D1 (de) 1994-12-01
AU1762088A (en) 1988-12-15
JPS63317770A (ja) 1988-12-26
AU601048B2 (en) 1990-08-30
IL86695A0 (en) 1988-11-30
EP0294996B1 (de) 1994-10-26
CA1299772C (en) 1992-04-28
EP0294996A2 (de) 1988-12-14
IL86695A (en) 1992-01-15
EP0294996A3 (de) 1991-04-10
US4833332A (en) 1989-05-23

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Owner name: NEN LIFE SCIENCE PRODUCTS, INC.(N.D.GES.DES STAATE