DE3855762T2 - Direkte Methode zum Nachweis von sehr niedrigerem Grad der Coliform-Kontamination - Google Patents

Direkte Methode zum Nachweis von sehr niedrigerem Grad der Coliform-Kontamination

Info

Publication number
DE3855762T2
DE3855762T2 DE3855762T DE3855762T DE3855762T2 DE 3855762 T2 DE3855762 T2 DE 3855762T2 DE 3855762 T DE3855762 T DE 3855762T DE 3855762 T DE3855762 T DE 3855762T DE 3855762 T2 DE3855762 T2 DE 3855762T2
Authority
DE
Germany
Prior art keywords
microorganisms
low levels
direct method
methylumbelliferone
substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE3855762T
Other languages
English (en)
Other versions
DE3855762D1 (de
Inventor
James D Berg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of DE3855762D1 publication Critical patent/DE3855762D1/de
Publication of DE3855762T2 publication Critical patent/DE3855762T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/20—Coumarin derivatives
    • C12Q2334/22—4-Methylumbelliferyl, i.e. beta-methylumbelliferone, 4MU
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/245—Escherichia (G)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli
DE3855762T 1987-11-05 1988-11-03 Direkte Methode zum Nachweis von sehr niedrigerem Grad der Coliform-Kontamination Expired - Fee Related DE3855762T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US11748187A 1987-11-05 1987-11-05

Publications (2)

Publication Number Publication Date
DE3855762D1 DE3855762D1 (de) 1997-02-27
DE3855762T2 true DE3855762T2 (de) 1997-05-07

Family

ID=22373184

Family Applications (2)

Application Number Title Priority Date Filing Date
DE3852825T Expired - Fee Related DE3852825T2 (de) 1987-11-05 1988-11-03 Schnellverfahren zum nachweis von coliformen mikroorganismen.
DE3855762T Expired - Fee Related DE3855762T2 (de) 1987-11-05 1988-11-03 Direkte Methode zum Nachweis von sehr niedrigerem Grad der Coliform-Kontamination

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE3852825T Expired - Fee Related DE3852825T2 (de) 1987-11-05 1988-11-03 Schnellverfahren zum nachweis von coliformen mikroorganismen.

Country Status (6)

Country Link
US (1) US5292644A (de)
EP (2) EP0386051B1 (de)
AT (2) ATE117378T1 (de)
AU (1) AU2602488A (de)
DE (2) DE3852825T2 (de)
WO (1) WO1989004372A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19841588A1 (de) * 1998-09-11 2000-03-23 Nitzsche Frank Verfahren zum Nachweis von Zellen in einer Probe

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DE3811097A1 (de) * 1988-03-31 1989-10-12 Orpegen Med Molekularbioforsch Verfahren zur steuerung biologischer klaerstufen
US5429933A (en) * 1986-06-30 1995-07-04 Edberg; Stephen C. Detection of first generation environmental sourced microbes in an environmentally-derived sample
IL85948A0 (en) * 1987-04-08 1988-09-30 Cambridge Bioscience Corp Method and kit for detecting bacteria or fungi
US5518894A (en) * 1987-11-05 1996-05-21 Berg; James D. Rapid coliform detection system
AU616957B2 (en) * 1988-06-20 1991-11-14 Becton Dickinson & Company Device for enhancing fluorescence and kinetics and methods of using the device
US6306621B1 (en) * 1991-11-18 2001-10-23 The United States Of America As Represented By The Administrator Of The U.S. Environmental Protection Agency Membrane filter agar medium for simultaneous detection of total coliforms and E. coli
US5650290A (en) * 1994-04-01 1997-07-22 Hach Company Method & Medium for use in detecting E. coli and total coliforms
US5849515A (en) * 1994-04-01 1998-12-15 Hach Company Method and medium for use in detecting E. coli and total coliforms
US5601998A (en) * 1994-08-18 1997-02-11 Minnesota Mining & Mfg Culture medium and device for detection and enumeration of enterobacteriaceae
CA2204121C (en) * 1994-11-04 2010-12-21 Elizabeth Ehrenfeld Medium for detecting target microbes in a sample
US5610029A (en) * 1994-11-04 1997-03-11 Idexx Laboratories, Inc. Medium for detecting target microbes in a sample
US5985594A (en) * 1995-11-14 1999-11-16 Idexx Laboratories, Inc. Method for quantification of biological material in a sample
US5700655A (en) * 1995-11-14 1997-12-23 Idexx Laboratories, Inc. Method for quantification of biological material in a sample
US7122338B2 (en) * 1995-11-14 2006-10-17 Biocontrol Systems, Inc. Method for quantification of biological material in a sample
IT1284658B1 (it) * 1996-06-03 1998-05-21 Isrim S C A R L Stimolatore dell'attivita' enzimatica in cellule batteriche
IT1284178B1 (it) * 1996-06-27 1998-05-08 Isrim S C A R L Procedimento per la rapida rilevazione in un campione della presenza di cellule batteriche anche se in numero limitatissimo.
US5843699A (en) * 1997-04-08 1998-12-01 Difco Laboratories, Inc. Rapid microorganism detection method
FR2769092B1 (fr) 1997-09-29 1999-11-12 Lyonnaise Eaux Eclairage Procede de regulation de la desinfection de liquides
AU8826598A (en) 1997-10-10 1999-05-03 President And Fellows Of Harvard College Surface-bound, double-stranded dna protein arrays
US5968762A (en) * 1998-03-19 1999-10-19 The University Of Connecticut Method for detecting bacteria in a sample
US5972641A (en) * 1998-08-28 1999-10-26 Colifast Systems Asa Rapid coliform detection system
US6511819B2 (en) 1998-08-28 2003-01-28 Nye Colifast As Rapid coliform detection system
US6723505B1 (en) 1999-08-13 2004-04-20 Nye Colifast As Method for identification of the indicators of contamination in liquid samples
US8021848B2 (en) 2001-09-06 2011-09-20 Straus Holdings Inc. Rapid and sensitive detection of cells and viruses
FR2829500B1 (fr) * 2001-09-13 2003-12-12 Hemosystem Procede de concentration et de detection de germes pathogenes a partir de produits sanguins et/ou de leurs derives et dispositif pour le mettre en oeuvre
JP3973876B2 (ja) * 2001-10-30 2007-09-12 株式会社三菱化学ヤトロン 新規の細菌分析方法
US20030138906A1 (en) * 2001-11-05 2003-07-24 Ingun Tryland Fluorescence test for measuring heterotrophic bacteria in water
US20030143658A1 (en) * 2002-01-28 2003-07-31 Casella Linda J. Richardson Rapid methods and devices for the detection of coliform and the detection and confirmation of E. coil
FR2845097B1 (fr) 2002-10-01 2006-06-16 Metis Biotechnologies Procede de detection et de comptage de microorganismes dans un echantillon
US7419797B2 (en) * 2002-10-17 2008-09-02 Conestoga - Rovers And Associates Limited Rapid coliform detection system
ITTR20030002A1 (it) * 2003-07-31 2005-02-01 Isrim Societa Consortile A Respon Sabilita Limit Metodo per la rilevazione di coliformi ed in particolare
DE05706799T1 (de) * 2004-03-01 2011-12-01 Mycometer A/S Kontaminationsmessung
DE102004011822A1 (de) * 2004-03-11 2005-09-29 Cognis Deutschland Gmbh & Co. Kg Qualitätssicherungssystem zum Nachweis von Mikroorganismen
US8373861B2 (en) * 2004-05-11 2013-02-12 Les Entreprises Biospec Global Solutions Inc. System for rapid analysis of microbiological materials in liquid samples
CA2895994A1 (en) 2004-06-23 2006-01-05 Zyzeba Testing Limited Improvements in and relating to micro-organism test apparatus and methods of using the same
FR2886651B1 (fr) * 2005-06-03 2007-09-21 Anjou Rech Procede de quantification rapide des micro-organismes coliformes vivants dans un echantillon d'eau
WO2007038478A2 (en) 2005-09-26 2007-04-05 Rapid Micro Biosystems, Inc Cassette containing growth medium
CA2738317C (en) 2008-09-24 2020-01-14 Straus Holdings Inc. Imaging analyzer for testing analytes
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
US20100112630A1 (en) * 2008-11-03 2010-05-06 Scott Martell Boyette Methods for measuring microbiological content in aqueous media
MX368123B (es) 2011-11-07 2019-09-19 Rapid Micro Biosystems Inc Cassette para pruebas de esterilidad.
CN104364388B (zh) 2012-04-16 2017-12-19 快速微型生物系统公司 细胞培养装置
DE102013225037B4 (de) 2013-12-05 2016-08-25 Asklepios Kliniken Verwaltungsgesellschaft mbH Verfahren zur Erkennung resistenter Keime und Vorrichtung zum Durchführen desselben
EP3740585B1 (de) 2018-01-17 2023-09-27 Bactobyte Ltd. Verbesserung der detektion von mikroorganismen

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US3870601A (en) * 1973-05-04 1975-03-11 Schering Corp Novel diagnostic system for differentiation of enterobacteriaceae
US4070247A (en) * 1977-03-30 1978-01-24 Indiana University Foundation Diagnostic media
FR2405301A1 (fr) * 1977-10-04 1979-05-04 Api Labor Substrats et procede permettant l'identification rapide de bacteries du genre streptococcus
US4242447A (en) * 1978-11-29 1980-12-30 Bioresearch Rapid detection of bacteria
JPH0321160B2 (de) * 1979-05-02 1991-03-22 Nat Res Dev
FR2457323A1 (fr) * 1979-05-25 1980-12-19 Api Labor Test permettant la mise en evidence des bacteries du genre salmonella et serratia et leur distinction des genres proteus et providencia
JPS5817598B2 (ja) * 1979-10-31 1983-04-08 味の素株式会社 微生物の迅速検出方法
GB2128204B (en) * 1982-04-14 1987-02-25 Unilever Plc Microbiological test processes and apparatus
US4693972A (en) * 1984-01-16 1987-09-15 Becton, Dickinson And Company Composition and method for rapid detection of microorganisms in clinical samples
FR2558847B1 (fr) * 1984-01-31 1986-06-20 Millipore Sa Dispositif et procede de controle microbiologique de liquides
EP0215066A1 (de) * 1985-02-27 1987-03-25 University Of Cincinnati Nachweis von lebensfähigen mikroorganismen durch induzierte fluoreszenz
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19841588A1 (de) * 1998-09-11 2000-03-23 Nitzsche Frank Verfahren zum Nachweis von Zellen in einer Probe

Also Published As

Publication number Publication date
EP0574977B1 (de) 1997-01-15
ATE117378T1 (de) 1995-02-15
WO1989004372A1 (en) 1989-05-18
DE3852825D1 (de) 1995-03-02
AU2602488A (en) 1989-06-01
ATE147790T1 (de) 1997-02-15
US5292644A (en) 1994-03-08
EP0386051A1 (de) 1990-09-12
DE3855762D1 (de) 1997-02-27
EP0386051B1 (de) 1995-01-18
EP0574977A1 (de) 1993-12-22
DE3852825T2 (de) 1995-05-18

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Legal Events

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8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee