DE60028819D1 - METHOD OF TREATING A MATRIX NUCLEIC ACID USING AN INTEGRATED MICROFLUIDIC PLATE - Google Patents

METHOD OF TREATING A MATRIX NUCLEIC ACID USING AN INTEGRATED MICROFLUIDIC PLATE

Info

Publication number
DE60028819D1
DE60028819D1 DE60028819T DE60028819T DE60028819D1 DE 60028819 D1 DE60028819 D1 DE 60028819D1 DE 60028819 T DE60028819 T DE 60028819T DE 60028819 T DE60028819 T DE 60028819T DE 60028819 D1 DE60028819 D1 DE 60028819D1
Authority
DE
Germany
Prior art keywords
nucleic acid
treating
integrated microfluidic
thermocycling reaction
microfluidic plate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60028819T
Other languages
German (de)
Other versions
DE60028819T2 (en
Inventor
Eric Tooke
Per Andersson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gyros Patent AB
Original Assignee
Gyros Patent AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP1999/010347 external-priority patent/WO2000040750A1/en
Priority claimed from GBGB0011425.6A external-priority patent/GB0011425D0/en
Application filed by Gyros Patent AB filed Critical Gyros Patent AB
Application granted granted Critical
Publication of DE60028819D1 publication Critical patent/DE60028819D1/en
Publication of DE60028819T2 publication Critical patent/DE60028819T2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B01L2300/0806—Standardised forms, e.g. compact disc [CD] format
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/088—Passive control of flow resistance by specific surface properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Abstract

Disclosed is a method for performing the steps of nucleic acid template purification, a thermocycling reaction and purification of the products of the thermocycling reaction characterised in that the steps take place sequentially in a microfluidic disc. Also disclosed is a microstructure for fluids comprising at least one inlet opening connected to a first chamber incorporating a means for purifying template nucleic acid which, in turn, is connected to a second chamber incorporating a means for a thermocycling reaction which, in turn, is connected to a third chamber incorporating a means for purifying products of the thermocycling reaction, and a microfluidic disc comprising a plurality of such microstructures.
DE60028819T 1999-12-23 2000-12-20 METHOD OF TREATING A MATRIX NUCLEIC ACID USING AN INTEGRATED MICROFLUIDIC PLATE Expired - Lifetime DE60028819T2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
WOPCT/EP99/10347 1999-12-23
PCT/EP1999/010347 WO2000040750A1 (en) 1998-12-30 1999-12-23 Method for sequencing dna using a microfluidic device
GB0011425 2000-05-12
GBGB0011425.6A GB0011425D0 (en) 1999-12-23 2000-05-12 Integrated microfluidic disc
PCT/EP2000/013014 WO2001047638A2 (en) 1999-12-23 2000-12-20 Integrated microfluidic disc

Publications (2)

Publication Number Publication Date
DE60028819D1 true DE60028819D1 (en) 2006-07-27
DE60028819T2 DE60028819T2 (en) 2007-01-11

Family

ID=26070393

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60028819T Expired - Lifetime DE60028819T2 (en) 1999-12-23 2000-12-20 METHOD OF TREATING A MATRIX NUCLEIC ACID USING AN INTEGRATED MICROFLUIDIC PLATE

Country Status (5)

Country Link
JP (1) JP4791670B2 (en)
AT (1) ATE329691T1 (en)
AU (1) AU3537001A (en)
DE (1) DE60028819T2 (en)
WO (1) WO2001047638A2 (en)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7014815B1 (en) 1998-10-30 2006-03-21 Burstein Technologies, Inc. Trackable optical discs with concurrently readable nonoperational features
EP1284817A2 (en) * 2000-05-15 2003-02-26 Tecan Trading AG Microfluidics devices and methods for performing cell based assays
US7087203B2 (en) 2000-11-17 2006-08-08 Nagaoka & Co., Ltd. Methods and apparatus for blood typing with optical bio-disc
US7026131B2 (en) 2000-11-17 2006-04-11 Nagaoka & Co., Ltd. Methods and apparatus for blood typing with optical bio-discs
WO2002046721A2 (en) 2000-12-08 2002-06-13 Burstein Technologies, Inc. Optical discs for measuring analytes
US7054258B2 (en) 2000-12-08 2006-05-30 Nagaoka & Co., Ltd. Optical disc assemblies for performing assays
US7091034B2 (en) 2000-12-15 2006-08-15 Burstein Technologies, Inc. Detection system for disk-based laboratory and improved optical bio-disc including same
US7429354B2 (en) 2001-03-19 2008-09-30 Gyros Patent Ab Structural units that define fluidic functions
EP1384076B1 (en) 2001-03-19 2012-07-25 Gyros Patent Ab Characterization of reaction variables
US20080288178A1 (en) 2001-08-24 2008-11-20 Applera Corporation Sequencing system with memory
US6726820B1 (en) 2001-09-19 2004-04-27 Applera Corporation Method of separating biomolecule-containing samples with a microdevice with integrated memory
US6919058B2 (en) 2001-08-28 2005-07-19 Gyros Ab Retaining microfluidic microcavity and other microfluidic structures
EP2283924B1 (en) 2001-08-28 2013-04-17 Gyros Patent Ab Inlet unit with means supporting liquid entrance into a microchannel structure
US7189368B2 (en) 2001-09-17 2007-03-13 Gyros Patent Ab Functional unit enabling controlled flow in a microfluidic device
CN100382870C (en) * 2001-12-28 2008-04-23 株式会社日立高新技术 Extractor, chemical analyzer and chemical analyzing method
US6532997B1 (en) 2001-12-28 2003-03-18 3M Innovative Properties Company Sample processing device with integral electrophoresis channels
JP4895504B2 (en) * 2002-04-30 2012-03-14 ユィロス・パテント・アクチボラグ Centralized microfluidic device (EA)
US7005265B1 (en) 2002-06-20 2006-02-28 Wenhong Fan Nonenzymatic catalytic signal amplification for nucleic acid hybridization assays
JP2004354364A (en) 2002-12-02 2004-12-16 Nec Corp Fine particle manipulating unit, chip mounted with the same and detector, and method for separating, capturing and detecting protein
US7390464B2 (en) 2003-06-19 2008-06-24 Burstein Technologies, Inc. Fluidic circuits for sample preparation including bio-discs and methods relating thereto
US7238269B2 (en) * 2003-07-01 2007-07-03 3M Innovative Properties Company Sample processing device with unvented channel
US7776272B2 (en) 2003-10-03 2010-08-17 Gyros Patent Ab Liquid router
US7837947B2 (en) * 2003-12-12 2010-11-23 3M Innovative Properties Company Sample mixing on a microfluidic device
SE0400181D0 (en) * 2004-01-29 2004-01-29 Gyros Ab Segmented porous and preloaded microscale devices
US7187286B2 (en) 2004-03-19 2007-03-06 Applera Corporation Methods and systems for using RFID in biological field
TWI295730B (en) * 2004-11-25 2008-04-11 Ind Tech Res Inst Microfluidic chip for sample assay and method thereof
US7947235B2 (en) 2005-04-14 2011-05-24 Gyros Ab Microfluidic device with finger valves
US10816563B2 (en) 2005-05-25 2020-10-27 Boehringer Ingelheim Vetmedica Gmbh System for operating a system for the integrated and automated analysis of DNA or protein
US9110044B2 (en) 2005-05-25 2015-08-18 Boehringer Ingelheim Vetmedica Gmbh System for the integrated and automated analysis of DNA or protein and method for operating said type of system
US20090075801A1 (en) * 2007-09-19 2009-03-19 Dalibor Hodko Counter-centrifugal force device
WO2012033765A1 (en) 2010-09-07 2012-03-15 The Arizona Board Of Regents On Behalf Of The University Of Arizona Microdroplet-manipulation systems and methods for automated execution of molecular biological protocols
US9993819B2 (en) 2014-12-30 2018-06-12 Stmicroelectronics S.R.L. Apparatus for actuating and reading a centrifugal microfluidic disk for biological and biochemical analyses, and use of the apparatus
BR112019006862A2 (en) 2016-10-07 2019-06-25 Boehringer Ingelheim Vetmedica Gmbh Analysis device and method for testing a sample
EP3523046B1 (en) 2016-10-07 2022-02-16 Boehringer Ingelheim Vetmedica GmbH Method and analysis system for testing a sample
KR20200115579A (en) 2018-01-30 2020-10-07 라이프 테크놀로지스 코포레이션 Instruments, devices and consumables for use in the workflow of smart molecular analysis systems

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5585069A (en) * 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
EP0862647A1 (en) * 1995-11-03 1998-09-09 Sarnoff Corporation Assay system and method for conducting assays
CA2239613A1 (en) * 1995-12-05 1997-06-12 Alec Mian Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system with on-board informatics
KR20000075815A (en) * 1997-02-28 2000-12-26 번스타인 리차드 Laboratory in a disk
US6013513A (en) * 1997-10-30 2000-01-11 Motorola, Inc. Molecular detection apparatus
GB9828785D0 (en) * 1998-12-30 1999-02-17 Amersham Pharm Biotech Ab Sequencing systems
AU5887000A (en) * 1999-06-22 2001-01-09 Tecan Trading Ag Devices and methods for the performance of miniaturized in vitro amplification assays

Also Published As

Publication number Publication date
AU3537001A (en) 2001-07-09
DE60028819T2 (en) 2007-01-11
WO2001047638A2 (en) 2001-07-05
ATE329691T1 (en) 2006-07-15
JP2003520582A (en) 2003-07-08
WO2001047638A3 (en) 2002-05-30
JP4791670B2 (en) 2011-10-12

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