DE60101939D1 - Mit stickstoffheterozyklen substituierte elektronenmangel-fluoresceinfarbstoffe - Google Patents

Mit stickstoffheterozyklen substituierte elektronenmangel-fluoresceinfarbstoffe

Info

Publication number
DE60101939D1
DE60101939D1 DE60101939T DE60101939T DE60101939D1 DE 60101939 D1 DE60101939 D1 DE 60101939D1 DE 60101939 T DE60101939 T DE 60101939T DE 60101939 T DE60101939 T DE 60101939T DE 60101939 D1 DE60101939 D1 DE 60101939D1
Authority
DE
Germany
Prior art keywords
fluorescine
inks
nitrogen heterocycles
substituted
default
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60101939T
Other languages
English (en)
Other versions
DE60101939T2 (de
Inventor
Krishna Upadhya
Steven Menchen
Weiguo Zhen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Publication of DE60101939D1 publication Critical patent/DE60101939D1/de
Application granted granted Critical
Publication of DE60101939T2 publication Critical patent/DE60101939T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/04—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/14—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/06—Hydroxy derivatives of triarylmethanes in which at least one OH group is bound to an aryl nucleus and their ethers or esters
    • C09B11/08—Phthaleins; Phenolphthaleins; Fluorescein
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
DE60101939T 2000-02-07 2001-02-05 Mit stickstoffheterozyklen substituierte elektronenmangel-fluoresceinfarbstoffe Expired - Lifetime DE60101939T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US498702 2000-02-07
US09/498,702 US6221604B1 (en) 2000-02-07 2000-02-07 Electron-deficient nitrogen heterocycle-substituted fluorescein dyes
PCT/US2001/003772 WO2001057264A2 (en) 2000-02-07 2001-02-05 Electron-deficient nitrogen heterocycle-substituted fluorescein dyes

Publications (2)

Publication Number Publication Date
DE60101939D1 true DE60101939D1 (de) 2004-03-11
DE60101939T2 DE60101939T2 (de) 2005-01-05

Family

ID=23982139

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60101939T Expired - Lifetime DE60101939T2 (de) 2000-02-07 2001-02-05 Mit stickstoffheterozyklen substituierte elektronenmangel-fluoresceinfarbstoffe

Country Status (9)

Country Link
US (2) US6221604B1 (de)
EP (1) EP1196640B1 (de)
JP (5) JP2003521571A (de)
AT (1) ATE258992T1 (de)
AU (1) AU772739B2 (de)
CA (1) CA2368495C (de)
DE (1) DE60101939T2 (de)
ES (1) ES2211768T3 (de)
WO (1) WO2001057264A2 (de)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6221604B1 (en) * 2000-02-07 2001-04-24 Pe Corporation Electron-deficient nitrogen heterocycle-substituted fluorescein dyes
US6811979B2 (en) * 2000-10-11 2004-11-02 Applera Corporation Fluorescent nucleobase conjugates having anionic linkers
US6887690B2 (en) * 2001-06-22 2005-05-03 Pe Corporation Dye-labeled ribonucleotide triphosphates
US8569516B2 (en) * 2001-09-07 2013-10-29 Elitech Holding B.V. Compounds and methods for fluorescent labeling
US6972339B2 (en) 2001-09-07 2005-12-06 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
WO2003040395A2 (en) * 2001-11-07 2003-05-15 Applera Corporation Universal nucleotides for nucleic acid analysis
US20030148284A1 (en) * 2001-12-17 2003-08-07 Vision Todd J. Solid phase detection of nucleic acid molecules
US7026166B2 (en) * 2002-01-22 2006-04-11 Chiron Corporation Fluorogenic dyes
US7074597B2 (en) * 2002-07-12 2006-07-11 The Trustees Of Columbia University In The City Of New York Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry
US7704756B2 (en) * 2003-01-21 2010-04-27 Novartis Vaccines And Diagnostics, Inc. Fluorogenic dyes
BRPI0408967B8 (pt) 2003-03-31 2021-07-27 Hoffmann La Roche kit e métodos para detecção de um ácido nucléico de vários membros do sorogrupo do vírus da encefalite japonesa em uma amostra biológica sob condições de hibridização rigorosas
US7399639B2 (en) * 2003-05-04 2008-07-15 Massachusetts Institute Of Technology Sensors, and methods of making and using the same
US20060029948A1 (en) * 2003-09-19 2006-02-09 Gary Lim Sealing cover and dye compatibility selection
US7570443B2 (en) 2003-09-19 2009-08-04 Applied Biosystems, Llc Optical camera alignment
US7417726B2 (en) * 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
US7541454B2 (en) * 2003-10-24 2009-06-02 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
WO2005056613A2 (en) * 2003-12-08 2005-06-23 The Research Foundation Of State University Of New York Site selectively tagged and templated molecularly imprinted polymers for sensor applications
JP4127204B2 (ja) * 2003-12-17 2008-07-30 セイコーエプソン株式会社 液晶表示装置の製造方法
AU2005205341B2 (en) * 2004-01-07 2009-11-19 The Research Foundation Of State University Of New York Protein imprinted polymers with integrated emission sites
WO2005077125A2 (en) * 2004-02-11 2005-08-25 Applera Corporation Methods and compositions for detecting nucleic acids
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
PL1748767T3 (pl) 2004-05-28 2012-08-31 Unigen Inc 1-(3-metylo-2,4-dimetoksyfenylo)-3-(2',4'-dihydroksyfenylo)-propan jako silny inhibitor tyrozynazy
US7767834B2 (en) * 2004-08-13 2010-08-03 Elitech Holding B.V. Phosphonylated fluorescent dyes and conjugates
WO2006020947A2 (en) 2004-08-13 2006-02-23 Epoch Biosciences, Inc. Phosphonate fluorescent dyes and conjugates
EP2312317A3 (de) 2005-05-03 2011-06-08 Life Technologies Corporation Fluoreszentes Erkennungssystem und Farbstoffsatz zur Verwendung damit
EP2674417A3 (de) * 2007-11-21 2014-04-09 Decode Genetics EHF Biaryl-PDE4-Inhibitoren zur Behandlung von Entzündungen
KR102397221B1 (ko) * 2008-07-21 2022-05-12 유니젠, 인크. 일련의 피부-화이트닝(라이트닝) 화합물
KR101718994B1 (ko) * 2009-09-16 2017-03-23 포항공과대학교 산학협력단 무기 및 유기수은종을 형광을 통하여 감지하는 화학량계의 개발
JP5958339B2 (ja) 2010-06-07 2016-07-27 コニカミノルタ株式会社 近接場増強蛍光センサチップ
WO2012023391A1 (ja) 2010-08-17 2012-02-23 コニカミノルタホールディングス株式会社 非特異吸着の精製機構を備えたｓｐｆｓ用センサ
EP2658998B1 (de) 2010-12-29 2015-04-22 Life Technologies Corporation Ddao-verbindungen als fluorezenzreferenzstandards
KR102113823B1 (ko) 2011-03-24 2020-05-21 유니젠, 인크. 디아릴프로판의 제조를 위한 화합물 및 방법
ES2570591T3 (es) 2011-05-24 2016-05-19 Elitechgroup B V Detección de Staphylococcus aureus resistente a meticilina
CN102321063B (zh) * 2011-07-19 2015-07-01 华东理工大学 一种制备不对称罗丹明的方法
EP2738556B1 (de) 2011-07-28 2019-11-06 Konica Minolta, Inc. Verfahren zum messen der menge eines analyten und vorrichtung für spfs
CN103012354A (zh) * 2012-12-24 2013-04-03 广州医药研究总院 5（6）-羧基荧光素异构体的制备方法
EP2971106B1 (de) 2013-03-11 2020-02-26 ELITechGroup, Inc. Verfahren für echte isothermale strangverschiebungsamplifikation
WO2014186147A2 (en) 2013-05-13 2014-11-20 Elitech Holding B.V. Droplet digital pcr with short minor groove probes
CN103508941B (zh) * 2013-10-29 2015-07-22 郑州大学 2,4-二苯基-5,6-二吡啶基-苯酚及其制备方法
EP3230467A1 (de) 2014-12-12 2017-10-18 ELITechGroup B.V. Verfahren und zusammensetzungen zum nachweis von antibiotikaresistenten bakterien
US9988670B2 (en) 2014-12-12 2018-06-05 Elitechgroup B.V. Methods and compositions for detecting antibiotic resistant bacteria
EP3247797B1 (de) 2015-01-12 2020-04-29 Life Technologies Corporation Verfahren, systeme und zusammensetzungen daraus zur kontrolle und quantifizierung der nukleinsäurebibliotheksqualität
JP6675758B2 (ja) * 2015-02-20 2020-04-01 国立大学法人名古屋大学 ホスファフルオレセイン化合物若しくはその塩、又はそれを用いた蛍光色素
WO2019069885A1 (ja) 2017-10-02 2019-04-11 コニカミノルタ株式会社 アナライトの検出方法
WO2020132487A1 (en) 2018-12-20 2020-06-25 Life Technologies Corporation Modified rhodamine dye and use thereof in biological assays
BR112021012154A2 (pt) 2018-12-20 2021-09-08 Life Technologies Corporation Corante de rodamina assimétrica e uso da mesma em ensaios biológicos
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
WO2021092408A1 (en) * 2019-11-08 2021-05-14 The University Of North Carolina At Charlotte Asymmetric donor-acceptor molecular dyes
US20230021481A1 (en) 2019-12-06 2023-01-26 Otsuka Pharmaceutical Co., Ltd. Standard substance for psa quantification, preparation method therefor, standard solution for psa quantification, and psa quantification method
US20240060117A1 (en) 2020-07-23 2024-02-22 Life Technologies Corporation Energy transfer dye conjugates for use in biological assays
KR20230056685A (ko) 2020-07-23 2023-04-27 라이프 테크놀로지스 코포레이션 염료를 사용한 생물학적 분석을 위한 조성물, 시스템 및 방법
CN113563360A (zh) * 2021-08-16 2021-10-29 中国科学技术大学 一种两亲性荧光染料及其合成方法和应用

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4318846A (en) 1979-09-07 1982-03-09 Syva Company Novel ether substituted fluorescein polyamino acid compounds as fluorescers and quenchers
US5047519A (en) 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5654442A (en) * 1989-11-14 1997-08-05 The Perkin-Elmer Corporation 4,7-dichlorofluorescein dyes as molecular probes
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
WO1994005688A1 (en) 1992-09-03 1994-03-17 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5453505A (en) 1994-06-30 1995-09-26 Biometric Imaging, Inc. N-heteroaromatic ion and iminium ion substituted cyanine dyes for use as fluorescence labels
US6020481A (en) * 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
US5863727A (en) 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5821356A (en) 1996-08-12 1998-10-13 The Perkin Elmer Corporation Propargylethoxyamino nucleotides
US5770716A (en) 1997-04-10 1998-06-23 The Perkin-Elmer Corporation Substituted propargylethoxyamido nucleosides, oligonucleotides and methods for using same
US6130101A (en) * 1997-09-23 2000-10-10 Molecular Probes, Inc. Sulfonated xanthene derivatives
US6008379A (en) 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
US6221604B1 (en) * 2000-02-07 2001-04-24 Pe Corporation Electron-deficient nitrogen heterocycle-substituted fluorescein dyes

Also Published As

Publication number Publication date
USRE39663E1 (en) 2007-05-29
WO2001057264A3 (en) 2002-02-21
ATE258992T1 (de) 2004-02-15
JP2003521571A (ja) 2003-07-15
US6221604B1 (en) 2001-04-24
JP2006070270A (ja) 2006-03-16
EP1196640A2 (de) 2002-04-17
JP4942150B2 (ja) 2012-05-30
CA2368495C (en) 2009-09-29
AU3483901A (en) 2001-08-14
ES2211768T3 (es) 2004-07-16
AU772739B2 (en) 2004-05-06
WO2001057264A2 (en) 2001-08-09
DE60101939T2 (de) 2005-01-05
JP2007277565A (ja) 2007-10-25
JP2007277564A (ja) 2007-10-25
CA2368495A1 (en) 2001-08-09
JP2006257427A (ja) 2006-09-28
EP1196640B1 (de) 2004-02-04

Similar Documents

Publication Publication Date Title
DE60101939D1 (de) Mit stickstoffheterozyklen substituierte elektronenmangel-fluoresceinfarbstoffe
ATE445615T1 (de) Pyrido(2,1-a)isochinolinderivative als dpp-iv- hemmer
MXPA03002572A (es) Secuencias de nucleotidos que intervienen en la fertilidad de los machos y metodo de su uso.
WO2003019145A3 (en) Non-fluorescent quencher compounds and biomolecular assays
WO2004038378A3 (en) Phthalocyanine dyes
DE60005787D1 (de) Wasserlösliche rhodaminfarbstoffe und ihre konjugate
ES2181006T3 (es) Uso de amidas de acidos de piridil alcanos, piridil alquenos y/o piridil alquinos en el tratamiento de tumores o en inmunosupresion.
DE60018105D1 (de) Polypeptidzusammensetzungen mit verbesserter stabilität
WO2001004265A3 (en) C-terminal protein tagging
WO2005047459A3 (en) Sars nucleic acids, proteins, antibodies, and uses thereof
WO2001036685A3 (en) Differential gene expression in cancer
WO1999024452A8 (en) Pyrimidine derivatives for labeled binding partners
DE60211949D1 (de) Abscheidung von kupfer mit kupferformatkomplexen
WO2004055117A3 (de) Carboxamid-substituierte farbstoffe für analytische anwendungen
ATE505549T1 (de) Männliche fertilitätvermittelnde nukleotidsequenzen und deren verwendung
PT1023310E (pt) Sintese melhorada de oligonucleotidos sulfurados
WO2005014723A8 (en) Cyanin-type compounds having an alkynyl linker arm
TR200101551T2 (tr) Farklı kütinaz şekilleri.
TR200501541T2 (tr) Leu-hirudinin e.coli ile kültür ortamında ayrışarak üretilmesinin sinyal sekansları
EP1543007A4 (de) Neue zwitterionische fluoreszierende farbstoffe zur markierung bei proteomischen und anderen biologischen analysen
WO2006100200A3 (de) Metallkomplexfarbstoffmischungen
CA2529001A1 (en) Binding peptides: methods for their generation and use
MXPA02012178A (es) Colorantes azo de hidroxipiridonmetido.
WO2001075163A3 (en) High throughput methods for haplotyping
WO2001066752A3 (en) Reproduction-specific genes

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: APPLIED BIOSYSTEMS, LLC (N. D. GES. D. STAATES, US

8380 Miscellaneous part iii

Free format text: PFANDRECHT

8380 Miscellaneous part iii

Free format text: PFANDRECHT AUFGEHOBEN