DE60107468D1 - Nucleinsäure-isolation - Google Patents

Nucleinsäure-isolation

Info

Publication number
DE60107468D1
DE60107468D1 DE60107468T DE60107468T DE60107468D1 DE 60107468 D1 DE60107468 D1 DE 60107468D1 DE 60107468 T DE60107468 T DE 60107468T DE 60107468 T DE60107468 T DE 60107468T DE 60107468 D1 DE60107468 D1 DE 60107468D1
Authority
DE
Germany
Prior art keywords
nucleic acid
charge
ionisable group
acid isolation
biological
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60107468T
Other languages
English (en)
Other versions
DE60107468T2 (de
Inventor
John Baker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
DNA Research Innovations Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DNA Research Innovations Ltd filed Critical DNA Research Innovations Ltd
Application granted granted Critical
Publication of DE60107468D1 publication Critical patent/DE60107468D1/de
Publication of DE60107468T2 publication Critical patent/DE60107468T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
DE60107468T 2000-12-14 2001-12-14 Nucleinsäure-isolation Expired - Lifetime DE60107468T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/736,632 US6914137B2 (en) 1997-12-06 2000-12-14 Isolation of nucleic acids
PCT/GB2001/005524 WO2002048164A2 (en) 2000-12-14 2001-12-14 Isolation of nucleic acids
US736632 2003-12-17

Publications (2)

Publication Number Publication Date
DE60107468D1 true DE60107468D1 (de) 2004-12-30
DE60107468T2 DE60107468T2 (de) 2005-12-29

Family

ID=24960633

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60107468T Expired - Lifetime DE60107468T2 (de) 2000-12-14 2001-12-14 Nucleinsäure-isolation

Country Status (10)

Country Link
US (8) US6914137B2 (de)
EP (2) EP1473299A3 (de)
JP (1) JP4868697B2 (de)
AT (1) ATE283275T1 (de)
AU (1) AU2002216203A1 (de)
CA (1) CA2432075A1 (de)
DE (1) DE60107468T2 (de)
DK (1) DK1345952T3 (de)
ES (1) ES2233560T3 (de)
WO (1) WO2002048164A2 (de)

Families Citing this family (252)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US6048734A (en) 1995-09-15 2000-04-11 The Regents Of The University Of Michigan Thermal microvalves in a fluid flow method
US20060003447A1 (en) * 2003-12-30 2006-01-05 Richard Fike Dry powder cells and cell culture reagents and methods of production thereof
US7078224B1 (en) * 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
EP1147226B1 (de) * 1999-01-27 2013-01-23 Folim G. Halaka Materialien und verfahren zur reinigung von polyelektrolyten
DE10006590B4 (de) * 2000-02-11 2007-10-18 Qiagen North American Holdings, Inc. Verwendung funktionalisierter Membranen bzw. Matrizes zur Aufreinigung von Nukleinsäuren sowie entsprechende Verfahren
EP1339829B1 (de) * 2000-11-06 2018-10-24 Life Technologies Corporation Zellkulturmedium in trockenpulverform und herstellungsverfahren dafür
EP1349951A4 (de) * 2001-01-09 2005-08-24 Whitehead Biomedical Inst Verfahren und reagenzien zur isolierung von nukleinsäuren
EP1360490B1 (de) * 2001-01-23 2011-12-21 President and Fellows of Harvard College Nukleinsäure-programmierbare protein-arrays
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7718354B2 (en) 2001-03-02 2010-05-18 Ibis Biosciences, Inc. Methods for rapid identification of pathogens in humans and animals
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US20040121313A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in organs for transplantation
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US7323140B2 (en) 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
US8073627B2 (en) 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
WO2003062462A2 (en) 2002-01-16 2003-07-31 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
GB0212826D0 (en) 2002-05-31 2002-07-10 Dna Res Innovations Ltd Materials and methods relating to polyions and substance delivery
US7597936B2 (en) * 2002-11-26 2009-10-06 University Of Utah Research Foundation Method of producing a pigmented composite microporous material
AU2003293015A1 (en) * 2002-11-26 2004-06-18 University Of Utah Research Foundation Microporous materials, methods, and articles for localizing and quantifying analytes
JP2006516193A (ja) 2002-12-06 2006-06-29 アイシス・ファーマシューティカルス・インコーポレーテッド ヒトおよび動物における病原体の迅速な同定方法
GB0229287D0 (en) * 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
US20040157219A1 (en) * 2003-02-06 2004-08-12 Jianrong Lou Chemical treatment of biological samples for nucleic acid extraction and kits therefor
US7601491B2 (en) * 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
US20040197780A1 (en) * 2003-04-02 2004-10-07 Agencourt Bioscience Corporation Method for isolating nucleic acids
US8046171B2 (en) 2003-04-18 2011-10-25 Ibis Biosciences, Inc. Methods and apparatus for genetic evaluation
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US7964343B2 (en) * 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
GB0317044D0 (en) * 2003-07-21 2003-08-27 Dna Res Innovations Ltd Nucleic acid isolation
EP2402089A1 (de) 2003-07-31 2012-01-04 Handylab, Inc. Verarbeitung partikelhaltiger Proben
US7329742B2 (en) * 2003-09-04 2008-02-12 The Regents Of The University Of California Aptamers and methods for their in vitro selection and uses thereof
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US7956175B2 (en) 2003-09-11 2011-06-07 Ibis Biosciences, Inc. Compositions for use in identification of bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
GB0323814D0 (en) * 2003-10-10 2003-11-12 Cobra Biolog Ltd Purification of biological macromolecules
US20050106577A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Cleavable solid phases for isolating nucleic acids
US20050106576A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Methods of using cleavable solid phases for isolating nucleic acids
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
MXPA05001815A (es) * 2004-02-20 2005-08-24 Hoffmann La Roche Adsorcion de acidos nucleicos a una fase solida.
US8119336B2 (en) 2004-03-03 2012-02-21 Ibis Biosciences, Inc. Compositions for use in identification of alphaviruses
WO2005108615A2 (en) * 2004-04-14 2005-11-17 President And Fellows Of Harvard College Nucleic-acid programmable protein arrays
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
ES2553097T3 (es) * 2004-05-03 2015-12-04 Handylab, Inc. Procesamiento de muestras que contienen polinucleótidos
AU2005243187A1 (en) * 2004-05-11 2005-11-24 Wyeth Oligonucleotide arrays to monitor gene expression and methods for making and using same
CA2567839C (en) 2004-05-24 2011-06-28 Isis Pharmaceuticals, Inc. Mass spectrometry with selective ion filtration by digital thresholding
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
EP1778877B1 (de) 2004-07-28 2009-10-21 Canon U.S. Life Sciences, Inc. Verfahren zur überwachung genomischer dna von organismen
JP2008510456A (ja) * 2004-07-30 2008-04-10 アジェンコート バイオサイエンス コーポレーション 多官能基コート化固相担体を用いる核酸の単離方法
AU2005271687A1 (en) * 2004-08-03 2006-02-16 Becton, Dickinson And Company Use of magnetic material to fractionate samples
AU2005271688B2 (en) * 2004-08-03 2011-10-06 Becton, Dickinson And Company Use of magnetic material to direct isolation of compounds and fractionation of multipart samples
US7541060B2 (en) * 2004-08-17 2009-06-02 Xerox Corporation Bichromal balls
WO2006135400A2 (en) 2004-08-24 2006-12-21 Isis Pharmaceuticals, Inc. Methods for rapid identification of recombinant organisms
KR100613734B1 (ko) * 2004-09-07 2006-08-22 굿젠 주식회사 키토산을 이용한 ｄｎａ 보관방법 및 분석방법 및 이를이용한 ｄｎａ 제품
EP1650297B1 (de) * 2004-10-19 2011-04-13 Samsung Electronics Co., Ltd. Verfahren und Apparatur zur schnellen Zerstörung von Zellen und Viren durch die Verwendung von mikro magnet Kügelchen und Laser
KR100624450B1 (ko) * 2004-12-10 2006-09-18 삼성전자주식회사 히드로겔을 이용한 생물분자의 분리 및 정제 방법
KR100647306B1 (ko) 2004-12-23 2006-11-23 삼성전자주식회사 아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법
KR100601983B1 (ko) * 2005-01-20 2006-07-18 삼성전자주식회사 시료 내 핵산 증폭 저해 물질의 제거 방법
US7964380B2 (en) 2005-01-21 2011-06-21 Argylia Technologies Nanoparticles for manipulation of biopolymers and methods of thereof
US20090215124A1 (en) * 2005-02-15 2009-08-27 Weidong Cao Nucleic acid isolation methods and materials and devices thereof
WO2006089154A1 (en) * 2005-02-18 2006-08-24 Gen-Probe Incorporated Sample preparation method incorporating an alkaline shock
EP1871903B1 (de) * 2005-02-18 2011-12-21 Canon U.S. Life Sciences, Inc. Vorrichtung und verfahren zum identifizieren genomischer dna von organismen
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
US8182992B2 (en) 2005-03-03 2012-05-22 Ibis Biosciences, Inc. Compositions for use in identification of adventitious viruses
DE102005015005A1 (de) 2005-04-01 2006-10-05 Qiagen Gmbh Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe
KR100657957B1 (ko) * 2005-04-12 2006-12-14 삼성전자주식회사 아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법 및 상기방법에 사용될 수 있는 핵산 분리용 고상 물질
US20070105181A1 (en) * 2005-05-04 2007-05-10 Invitrogen Corporation Identification of cancer biomarkers and phosphorylated pdroteins
KR100668337B1 (ko) * 2005-05-21 2007-01-12 삼성전자주식회사 단백질에 비하여 핵산에 대한 결합력이 선택적으로 높은ｐＨ 의존성 이온 교환물질, 그가 고정화되어 있는 고체기판, 및 상기 물질 및 고체 기판을 이용하여 핵산을분리하는 방법
KR100668338B1 (ko) 2005-05-21 2007-01-12 삼성전자주식회사 신규한 ｐＨ 의존성 이온 교환물질, 그가 고정화되어 있는고체 기판, 및 상기 물질 및 고체 기판을 이용하여 핵산을분리하는 방법
WO2006130632A2 (en) * 2005-05-31 2006-12-07 Invitrogen Corporation Separation and purification of nucleic acid from paraffin-containing samples
US20070015191A1 (en) * 2005-07-01 2007-01-18 Promega Corporation Network of buoyant particles for biomolecule purification and use of buoyant particles or network of buoyant particles for biomolecule purification
AU2006272776B2 (en) 2005-07-21 2012-01-19 Ibis Biosciences, Inc. Methods for rapid identification and quantitation of nucleic acid variants
EP1941035B1 (de) 2005-09-01 2017-11-22 Canon U.S. Life Sciences, Inc. Verfahren und molekulardiagnostische vorrichtung zum nachweis, zur analyse und zur identifizierung genomischer dns
DE102005047736B4 (de) * 2005-09-29 2008-08-14 Aj Innuscreen Gmbh Verfahren und System zur Isolierung von Nukleinsäuren aus beliebigen komplexen Ausgangsmaterialien
US8916375B2 (en) * 2005-10-12 2014-12-23 University Of Virginia Patent Foundation Integrated microfluidic analysis systems
WO2007070381A2 (en) * 2005-12-09 2007-06-21 Promega Corporation Nucleic acid purification with a binding matrix
KR100829574B1 (ko) * 2006-01-03 2008-05-14 삼성전자주식회사 마이크로어레이용 기판, 그 마이크로어레이용 기판을이용하여 생분자를 분석하는 방법, 및 그 마이크로어레이용기판을 포함하는 랩온어칩
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
CN101389954A (zh) * 2006-02-23 2009-03-18 世诺临床诊断制品株式会社 金属的比色测定方法和测定试剂
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
EP2001990B1 (de) 2006-03-24 2016-06-29 Handylab, Inc. Integriertes system zur verarbeitung von mikrofluidischen proben und verwendungsverfahren
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8088616B2 (en) 2006-03-24 2012-01-03 Handylab, Inc. Heater unit for microfluidic diagnostic system
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
KR100785011B1 (ko) 2006-04-07 2007-12-11 삼성전자주식회사 쌍이온 화합물을 이용한 핵산 혼성화 특이성을 증가시키는방법
EP2010910A2 (de) * 2006-04-21 2009-01-07 Wyeth Profilbestimmungsanalyse der differentiellen expression von zellkulturphänotypen und verwendung damit
US8178316B2 (en) * 2006-06-29 2012-05-15 President And Fellows Of Harvard College Evaluating proteins
EP1882739A1 (de) * 2006-06-30 2008-01-30 Qiagen GmbH Verfahren zur Extraktion von Nukleinsäuren
US20080026374A1 (en) * 2006-07-31 2008-01-31 Sigma Aldrich Co. Compositions and Methods for Isolation of Biological Molecules
US20080023395A1 (en) * 2006-07-31 2008-01-31 Sigma Aldrich Co. Compositions and Methods for Isolation of Biological Molecules
US20080026375A1 (en) * 2006-07-31 2008-01-31 Sigma Aldrich Co. Compositions and Methods for Isolation of Biological Molecules
JP2009545317A (ja) * 2006-08-01 2009-12-24 アプライド バイオシステムズ， エルエルシー 分析物および核酸の検出
JP5233176B2 (ja) * 2006-08-04 2013-07-10 ソニー株式会社 燃料電池および電子機器
EP2064332B1 (de) 2006-09-14 2012-07-18 Ibis Biosciences, Inc. Gezielte gesamtgenomamplifizierung zur identifizierung von krankheitserregern
WO2008035991A2 (en) * 2006-09-19 2008-03-27 Michael Ronald Cook A nucleic acid extraction method
KR100862660B1 (ko) * 2006-09-25 2008-10-10 삼성전자주식회사 단일 비드에 의한 핵산의 분리 및 정제 방법 및 장치
EP1911844A1 (de) * 2006-10-10 2008-04-16 Qiagen GmbH Verfahren und Zusammenstellung zur Isolierung von Nukleinsäure
WO2008061165A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
US7492312B2 (en) * 2006-11-14 2009-02-17 Fam Adly T Multiplicative mismatched filters for optimum range sidelobe suppression in barker code reception
US20080131954A1 (en) * 2006-11-30 2008-06-05 Canon U.S. Life Sciences, Inc. Method of Separating Target DNA from Mixed DNA
US20080131955A1 (en) * 2006-11-30 2008-06-05 Canon U.S. Life Sciences, Inc. Method of Separating Target DNA from Mixed DNA
EP2066709B1 (de) * 2006-12-21 2012-04-11 Invitrogen Dynal AS Partikel und ihre verwendung in einem verfahren zur nukleinsäureisolierung oder einem verfahren zur phosphorproteinisolierung
US8163886B2 (en) * 2006-12-21 2012-04-24 Emd Millipore Corporation Purification of proteins
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US8999636B2 (en) 2007-01-08 2015-04-07 Toxic Report Llc Reaction chamber
WO2008104002A2 (en) 2007-02-23 2008-08-28 Ibis Biosciences, Inc. Methods for rapid forensic dna analysis
DE102007009347B4 (de) 2007-02-27 2009-11-26 Agowa Gmbh Verfahren zur Isolierung von Nukleinsäuren
CA2629589C (en) 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
JP5205818B2 (ja) * 2007-06-05 2013-06-05 ソニー株式会社 燃料電池および電子機器
WO2008152841A1 (ja) * 2007-06-13 2008-12-18 Sony Corporation 燃料電池および電子機器
US20090017460A1 (en) * 2007-06-15 2009-01-15 Wyeth Differential expression profiling analysis of cell culture phenotypes and uses thereof
US20090061497A1 (en) * 2007-06-29 2009-03-05 Becton, Dickinson And Company Methods for Extraction and Purification of Components of Biological Samples
US20100294665A1 (en) * 2007-07-12 2010-11-25 Richard Allen Method and system for transferring and/or concentrating a sample
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US20090136385A1 (en) 2007-07-13 2009-05-28 Handylab, Inc. Reagent Tube
WO2009012185A1 (en) 2007-07-13 2009-01-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
USD621060S1 (en) 2008-07-14 2010-08-03 Handylab, Inc. Microfluidic cartridge
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US20090048439A1 (en) * 2007-08-06 2009-02-19 Weisburg William G Isolation of nucleic acids molecules using modified solid supports
JP5298479B2 (ja) 2007-08-17 2013-09-25 ソニー株式会社 燃料電池および電子機器
US20110300608A1 (en) * 2007-09-21 2011-12-08 Streck, Inc. Nucleic acid isolation in preserved whole blood
US20090137024A1 (en) * 2007-11-28 2009-05-28 Canon U.S. Life Sciences, Inc. Method of Separating Target DNA from Mixed DNA
US20090186358A1 (en) * 2007-12-21 2009-07-23 Wyeth Pathway Analysis of Cell Culture Phenotypes and Uses Thereof
JP2009245920A (ja) * 2008-03-11 2009-10-22 Sony Corp 燃料電池、電子機器および燃料電池用緩衝液
JP2009245930A (ja) * 2008-03-12 2009-10-22 Sony Corp 燃料電池およびその製造方法ならびに酵素固定化電極およびその製造方法ならびに電子機器
USD618820S1 (en) 2008-07-11 2010-06-29 Handylab, Inc. Reagent holder
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
EP2315848B1 (de) 2008-07-18 2014-12-10 Canon U.S. Life Sciences, Inc. Verfahren und systeme für mikrofluidische dna-probenvorbereitung
US8304185B2 (en) 2009-07-17 2012-11-06 Canon U.S. Life Sciences, Inc. Methods and systems for DNA isolation on a microfluidic device
US10718909B2 (en) 2008-07-29 2020-07-21 Glenair, Inc. Expanded beam fiber optic connection system
GB0814570D0 (en) * 2008-08-08 2008-09-17 Diagnostics For The Real World Isolation of nucleic acid
EP2157181A1 (de) 2008-08-13 2010-02-24 AGOWA Gesellschaft für molekularbiologische Technologie mbH Verfahren zur Isolation von Nukleinsäuren und Testkit
US20110152510A1 (en) * 2008-08-25 2011-06-23 Ge Healthcare Bio-Sciences Corp. Simple load and elute process for purification of genomic dna
WO2010025302A2 (en) 2008-08-27 2010-03-04 Life Technologies Corporation Apparatus for and method of processing biological samples
US11235323B2 (en) 2008-08-27 2022-02-01 Life Technologies Corporation Apparatus for and method of processing biological samples
KR101015502B1 (ko) * 2008-09-09 2011-02-22 삼성전자주식회사 제1 ｐＨ에서 양전하 및 제2 ｐＨ에서 음전하를 띠는 화합물 및 그를 이용하여 핵산을 분리하는 방법
EP2344893B1 (de) 2008-09-16 2014-10-15 Ibis Biosciences, Inc. Mikroplatten-handhabungssysteme und verfahren
EP2347254A2 (de) 2008-09-16 2011-07-27 Ibis Biosciences, Inc. Probenverarbeitungseinheiten, -systeme und damit verbundene verfahren
EP2349549B1 (de) 2008-09-16 2012-07-18 Ibis Biosciences, Inc. Mischpatronen, mischstationen und verwandte ausrüstungen, und system
DE102008047790A1 (de) 2008-09-17 2010-04-15 Qiagen Gmbh Verfahren zur Normierung des Gehalts von Biomolekülen in einer Probe
US8361716B2 (en) * 2008-10-03 2013-01-29 Pathogenetix, Inc. Focusing chamber
KR101569832B1 (ko) * 2008-11-19 2015-11-18 삼성전자주식회사 시료 중의 게놈 ｄｎａ 및 플라스미드 ｄｎａ를 서로 분리하는 방법 및 그를 위한 키트
WO2010074953A1 (en) 2008-12-16 2010-07-01 Millipore Corporation Stirred tank reactor and method
DE102008063003A1 (de) * 2008-12-23 2010-06-24 Qiagen Gmbh Nukleinsäureaufreinigungsverfahren
DE102008063001A1 (de) * 2008-12-23 2010-06-24 Qiagen Gmbh Nukleinsäureaufreinigungsverfahren
CA2751455C (en) 2009-02-03 2019-03-12 Netbio, Inc. Nucleic acid purification
EP2396803A4 (de) 2009-02-12 2016-10-26 Ibis Biosciences Inc Ionisationssondenanordnungen
EP2256195A1 (de) * 2009-05-12 2010-12-01 Qiagen GmbH Nukleinsäureaufreinigungsverfahren
GB2473868A (en) 2009-09-28 2011-03-30 Invitrogen Dynal As Apparatus and method of automated processing of biological samples
WO2011008972A1 (en) 2009-07-17 2011-01-20 Ibis Biosciences, Inc. Systems for bioagent identification
US8950604B2 (en) 2009-07-17 2015-02-10 Ibis Biosciences, Inc. Lift and mount apparatus
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) * 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
EP2480666B1 (de) 2009-09-24 2017-03-08 QIAGEN Gaithersburg, Inc. Verfahren und Kits zur Isolierung von Nukleinsäuren mittels Anionentauschmatrix
EP2851399B1 (de) * 2009-09-29 2017-08-16 Covalon Technologies Inc. System und Verfahren zur Beschichtung medizinischer Vorrichtungen
EP3225695A1 (de) 2009-10-15 2017-10-04 Ibis Biosciences, Inc. Multiple-displacement-amplifikation
TWI407994B (zh) 2009-10-22 2013-09-11 Ind Tech Res Inst 分離核酸的方法、試劑及套組
EP2513335A4 (de) * 2009-12-14 2013-09-11 Betty Wu Verfahren und materialien zur abscheidung von nukleinsäurematerialien
US20130203606A1 (en) 2010-02-25 2013-08-08 Advanced Liquid Logic Inc Method of Preparing a Nucleic Acid Library
US8691918B2 (en) 2010-05-17 2014-04-08 Emd Millipore Corporation Stimulus responsive polymers for the purification of biomolecules
EP2576779B1 (de) 2010-06-01 2017-08-09 Qiagen GmbH Verfahren zum Isolieren und/oder Reinigen von Nukleinsäure(n)
CN101956000A (zh) * 2010-07-19 2011-01-26 博奥生物有限公司 可控释放生物分子的方法及可控释放生物分子的生物芯片
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
BR112013026451B1 (pt) 2011-04-15 2021-02-09 Becton, Dickinson And Company sistema e método para realizar ensaios de diagnóstico molecular em várias amostras em paralelo e simultaneamente amplificação em tempo real em pluralidade de câmaras de reação de amplificação
US10407713B2 (en) 2011-07-04 2019-09-10 Qiagen Gmbh Reagent usable for the isolation and/or purification of nucleic acids
JP5924888B2 (ja) * 2011-08-26 2016-05-25 関東化學株式会社 核酸抽出方法、核酸抽出試薬キットおよび核酸抽出用試薬
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
JP5899731B2 (ja) 2011-09-13 2016-04-06 ソニー株式会社 核酸精製方法、核酸抽出方法、及び核酸精製用キット
CN103930546A (zh) * 2011-09-26 2014-07-16 凯杰有限公司 用于分离细胞外核酸的快速方法
EP2760999A1 (de) 2011-09-26 2014-08-06 Qiagen GmbH Verfahren zur trennung von nukleinsäuren nach grösse
EP2761305B1 (de) 2011-09-30 2017-08-16 Becton, Dickinson and Company Vereinheitlichter reagenzienstreifen
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
US9340828B2 (en) 2011-10-27 2016-05-17 Ge Healthcare Bio-Sciences Ab Purification of nucleic acid
EP2773892B1 (de) 2011-11-04 2020-10-07 Handylab, Inc. Vorrichtung zur vorbereitung von polynukleotidproben
AU2013214849B2 (en) 2012-02-03 2016-09-01 Becton, Dickinson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests
US9637775B2 (en) 2012-02-13 2017-05-02 Neumodx Molecular, Inc. System and method for processing biological samples
US11648561B2 (en) 2012-02-13 2023-05-16 Neumodx Molecular, Inc. System and method for processing and detecting nucleic acids
US9604213B2 (en) 2012-02-13 2017-03-28 Neumodx Molecular, Inc. System and method for processing and detecting nucleic acids
US9339812B2 (en) 2012-02-13 2016-05-17 Neumodx Molecular, Inc. System and method for processing and detecting nucleic acids
US8685708B2 (en) 2012-04-18 2014-04-01 Pathogenetix, Inc. Device for preparing a sample
WO2013159116A1 (en) 2012-04-20 2013-10-24 University Of Chicago Fluidic devices for biospecimen preservation
JP6196661B2 (ja) 2012-04-20 2017-09-13 スリップチップ， エルエルシー サンプル調製または自律分析のための流体デバイスおよびシステム
US9803237B2 (en) 2012-04-24 2017-10-31 California Institute Of Technology Slip-induced compartmentalization
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
WO2014015269A1 (en) 2012-07-19 2014-01-23 Ariosa Diagnostics, Inc. Multiplexed sequential ligation-based detection of genetic variants
US9957553B2 (en) 2012-10-24 2018-05-01 Genmark Diagnostics, Inc. Integrated multiplex target analysis
US20140322706A1 (en) 2012-10-24 2014-10-30 Jon Faiz Kayyem Integrated multipelx target analysis
CN103789297A (zh) * 2012-10-26 2014-05-14 上海医脉赛科技有限公司 快速纯化核酸的方法及试剂盒
GB201304797D0 (en) 2013-03-15 2013-05-01 Diagnostics For The Real World Ltd Apparatus and method for automated sample preparation and adaptor for use in the apparatus
CN105228748B (zh) 2013-03-15 2017-10-10 金马克诊断股份有限公司 用于操纵可变形流体容器的系统、方法和设备
US9670479B2 (en) 2013-03-15 2017-06-06 F Cubed, LLC Sample preparation device and methods of use
USD881409S1 (en) 2013-10-24 2020-04-14 Genmark Diagnostics, Inc. Biochip cartridge
US9498778B2 (en) 2014-11-11 2016-11-22 Genmark Diagnostics, Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
US9719082B2 (en) 2013-10-31 2017-08-01 General Electric Company Substrates and associated methods for elution of nucleic acids
KR102207922B1 (ko) 2014-03-06 2021-01-26 삼성전자주식회사 반코마이신 저항성 엔테로코커스 특이적인 프라이머 세트, 그를 포함하는 조성물 및 시료 중 반코마이신 저항성 엔테로코커스 속 미생물을 검출하는 방법
WO2015195949A2 (en) 2014-06-18 2015-12-23 Clear Gene, Inc. Methods, compositions, and devices for rapid analysis of biological markers
JP2017539080A (ja) 2014-10-23 2017-12-28 コーニング インコーポレイテッド 高分子被包磁性ナノ粒子
KR102287811B1 (ko) 2014-10-31 2021-08-09 삼성전자주식회사 2개 표면을 결합시키는 방법 및 그에 의하여 제조된 구조체, 및 상기 구조체를 포함하는 미세유동 장치
US9598722B2 (en) 2014-11-11 2017-03-21 Genmark Diagnostics, Inc. Cartridge for performing assays in a closed sample preparation and reaction system
US10005080B2 (en) 2014-11-11 2018-06-26 Genmark Diagnostics, Inc. Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation
WO2016077294A1 (en) 2014-11-14 2016-05-19 Corning Incorporated Methods and kits for post-ivt rna purification
JP6709799B2 (ja) * 2015-03-24 2020-06-17 スリーエム イノベイティブ プロパティズ カンパニー 生物学的組成物の精製方法及びそのための物品
EP3885438A1 (de) 2015-06-10 2021-09-29 QIAGEN GmbH Verfahren zur isolierung extrazellulärer nukleinsäuren mittels anionenaustauschpartikeln
JP6675167B2 (ja) * 2015-08-28 2020-04-01 シスメックス株式会社 ペプチドの遊離方法及び回収方法、並びにペプチド遊離剤及び試薬キット
CA3008273A1 (en) 2015-12-18 2017-06-22 Clear Gene, Inc. Methods, compositions, kits and devices for rapid analysis of biological markers
WO2017151195A1 (en) * 2016-02-29 2017-09-08 The Penn State Research Foundation Nucleic acid molecular diagnosis
US20170298413A1 (en) * 2016-04-13 2017-10-19 New York Genome Center Methods for the isolation of biomolecules and uses thereof
US11161107B2 (en) 2016-05-25 2021-11-02 Integrated Micro-Chromatography Systems, Inc. Dispersive pipette extraction system for purification of large biomolecules
WO2018031739A1 (en) 2016-08-10 2018-02-15 New York Genome Center, Inc. Ultra-low coverage genome sequencing and uses thereof
EP3570974B1 (de) * 2017-01-20 2021-03-31 Dionex Corporation Multimodale chromatografische medien zur proteintrennung
CN110506203A (zh) 2017-04-07 2019-11-26 塞奇科学股份有限公司 用于通过使用集成电泳dna纯化来检测遗传结构变异的系统和方法
CN111094564A (zh) 2017-07-12 2020-05-01 伊鲁米纳公司 核酸提取材料、系统和方法
GB201819726D0 (en) 2018-12-03 2019-01-16 Diagnostics For The Real World Ltd HCV detection
GB2580385B (en) * 2019-01-08 2022-10-12 Quantumdx Group Ltd Oligonucleotide deposition onto polypropylene substrates
GB2580384B (en) * 2019-01-08 2021-01-27 Quantumdx Group Ltd Oligonucleotide deposition onto polypropylene substrates
JP2020124129A (ja) * 2019-02-01 2020-08-20 積水化学工業株式会社 核酸の単離方法及び核酸単離用デバイス
CN114787349A (zh) 2019-12-16 2022-07-22 凯杰有限公司 富集方法
CA3161785A1 (en) 2019-12-18 2021-06-24 Alexander Vlassov Systems, methods, and devices for automated nucleic acid and protein isolation
WO2021191795A1 (en) 2020-03-23 2021-09-30 Diagnostics For The Real World, Ltd Coronavirus detection
EP4168545A1 (de) * 2020-06-17 2023-04-26 Entegris, Inc. Ionenaustauschmembranen, filter und verfahren
JP2023535332A (ja) * 2020-07-20 2023-08-17 インスメッド インコーポレイテッド 好中球セリンプロテアーゼを抽出するおよびジペプチジルペプチダーゼ１媒介性病態を治療するための方法
GB202017751D0 (en) 2020-11-10 2020-12-23 Life Tech As Sample protection
KR20220075978A (ko) 2020-11-30 2022-06-08 (주)바이오니아 중저 비유전율을 가진 화합물을 포함한 핵산 결합 완충액을 이용한 핵산 분리방법
WO2022263500A1 (en) 2021-06-17 2022-12-22 Qiagen Gmbh Method for isolating non-vesicular mirna
WO2023084369A1 (en) 2021-11-12 2023-05-19 3M Innovative Properties Company Method of processing polynucleic acids
EP4215613A1 (de) * 2022-01-24 2023-07-26 Sartorius BIA Separations d.o.o. Verfahren zur trennung von einzelsträngigen rna-molekülen von anderen nukleinsäurespezies, verwendung einer festphase dafür und verfahren zur trennung von doppelsträngigen rna-molekülen nach grösse
US20230382875A1 (en) * 2022-04-22 2023-11-30 Promega Corporation Compounds, compositions, and methods for isolation of nucleic acids

Family Cites Families (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4055469A (en) * 1976-12-10 1977-10-25 Eastman Kodak Company Purification of microbial enzyme extracts using synthetic polyelectrolytes
US4648975A (en) * 1983-08-17 1987-03-10 Pedro B. Macedo Process of using improved silica-based chromatographic supports containing additives
US4843012A (en) 1986-09-17 1989-06-27 Genetics Institute, Inc. Novel composition for nucleic acid purification
DE3639949A1 (de) 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US5599667A (en) * 1987-03-02 1997-02-04 Gen-Probe Incorporated Polycationic supports and nucleic acid purification separation and hybridization
JPH01125395A (ja) 1987-07-29 1989-05-17 Life Technol Inc 核酸浦獲試薬
US4921805A (en) 1987-07-29 1990-05-01 Life Technologies, Inc. Nucleic acid capture method
US4908318A (en) 1987-09-04 1990-03-13 Integrated Genetics, Inc. Nucleic acid extraction methods
US4923978A (en) 1987-12-28 1990-05-08 E. I. Du Pont De Nemours & Company Process for purifying nucleic acids
JP2791367B2 (ja) 1988-04-21 1998-08-27 マイクロプローブ・コーポレーション 核酸抽出方法
IE883226L (en) 1988-10-25 1990-04-25 Dna Prep Galway Ltd S 22 Separation of nucleic acids from protein material using¹cation exchangers
US5512439A (en) * 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
WO1990008159A1 (en) 1989-01-23 1990-07-26 Invitron Corporation Method for removing dna from protein preparations
NL8900725A (nl) 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5334499A (en) 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
US5124444A (en) 1989-07-24 1992-06-23 Microprobe Corporation Lactam-containing compositions and methods useful for the extraction of nucleic acids
US5128247A (en) 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
US5641628A (en) 1989-11-13 1997-06-24 Children's Medical Center Corporation Non-invasive method for isolation and detection of fetal DNA
GB9003253D0 (en) 1990-02-13 1990-04-11 Amersham Int Plc Precipitating polymers
IT1240870B (it) 1990-02-14 1993-12-17 Talent Procedimento per l'estrazione e la purificazione di dna genomico umano
JPH06510363A (ja) 1990-10-29 1994-11-17 ディカルブ　プラント　ジェネティクス 磁気性粒子を使用する生物学的材料の単離
US5654179A (en) 1990-11-14 1997-08-05 Hri Research, Inc. Nucleic acid preparation methods
US5204246A (en) 1990-12-26 1993-04-20 Pioneer Hi-Bred International, Inc. Dna isolation method
CA2067711C (en) 1991-05-03 2000-08-08 Daniel Lee Woodard Solid phase extraction purification of dna
WO1993003167A1 (en) 1991-08-06 1993-02-18 The World Health Organisation Method of isolation of dna
AT397510B (de) * 1991-11-06 1994-04-25 Wimmer Theodor Verfahren zur herstellung von fettsäureestern kurzkettiger alkohole
US6051380A (en) 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US5710028A (en) * 1992-07-02 1998-01-20 Eyal; Nurit Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
GB9223334D0 (en) * 1992-11-06 1992-12-23 Hybaid Ltd Magnetic solid phase supports
GB2282138B (en) 1993-09-28 1997-09-03 Tosoh Corp Method of extracting nucleic acids and method of detecting specified nucleic acid sequences
GB9323305D0 (en) 1993-11-11 1994-01-05 Medinnova Sf Isoaltion of nucleic acid
GB2284208A (en) * 1993-11-25 1995-05-31 Pna Diagnostics As Nucleic acid analogues with a chelating functionality for metal ions
DE4432654C2 (de) 1994-09-14 1998-03-26 Qiagen Gmbh Verfahren zur Isolierung von Nukleinsäuren aus natürlichen Quellen
CN1150431A (zh) 1994-04-08 1997-05-21 海布里顿公司 用阴离子交换层析法纯化寡脱氧核苷酸硫代磷酸酯
US5622822A (en) 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5582988A (en) * 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5652348A (en) 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
US5660984A (en) 1994-12-09 1997-08-26 Davis; Thomas E. DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof
WO1996018732A2 (en) 1994-12-15 1996-06-20 Board Of Trustees Of The University Of Illinois Sequence-specific inhibition of dna synthesis by triplex-forming oligonucleotides
US5631146A (en) 1995-01-19 1997-05-20 The General Hospital Corporation DNA aptamers and catalysts that bind adenosine or adenosine-5'-phosphates and methods for isolation thereof
AR003122A1 (es) 1995-05-19 1998-07-08 Merck & Co Inc Un proceso para aislacion y purificacion de plasmidos en fermentadores de gran escala y el adn aislado y purificado obtenido mediante dicho proceso.
EP0830364A1 (de) 1995-06-08 1998-03-25 Progen Industries Limited Verfahren und vorrichtung zur dns-extraktion
US5783686A (en) 1995-09-15 1998-07-21 Beckman Instruments, Inc. Method for purifying nucleic acids from heterogenous mixtures
US5612473A (en) 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
DE29601618U1 (de) 1996-01-31 1996-07-04 Invitek Gmbh Vorrichtung zur gleichzeitigen multiplen Isolierung
US5981735A (en) * 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
SE9600590D0 (sv) 1996-02-19 1996-02-19 Pharmacia Biotech Ab Sätt för kromatografisk separation av peptider och nukleinsyra samt ny högaffin jonbytesmatris
US5916746A (en) * 1996-05-09 1999-06-29 Kirkegaard & Perry Laboratories, Inc. Formazan-based immunoassay
US5981235A (en) 1996-07-29 1999-11-09 Promega Corporation Methods for isolating nucleic acids using alkaline protease
US5874221A (en) * 1996-08-28 1999-02-23 The United States Of America As Represented By The Secretary Of Agriculture Species specific method for the PCR detection of phythophthora
CA2214495C (en) 1996-09-25 2002-02-05 Daniel L. Woodard Hydrated zirconium silicate composition for purification of nucleic acids
US5795748A (en) 1996-09-26 1998-08-18 Becton Dickinson And Company DNA microwell device and method
US6060246A (en) * 1996-11-15 2000-05-09 Avi Biopharma, Inc. Reagent and method for isolation and detection of selected nucleic acid sequences
EP0853123A1 (de) 1997-01-10 1998-07-15 Roche Diagnostics GmbH Reinigung von DNA durch Cross-Flow-Filtration
US5770712A (en) 1997-03-14 1998-06-23 Virginia Tech Intellectual Properties, Inc. Crosslinked hydrogel beads from chitosan
EP0897978A3 (de) 1997-08-22 2001-10-17 Becton, Dickinson and Company Zirkoniumoxyd und verwandten Verbindungen zur Reinigung von Nukleinsäuren
JP4304348B2 (ja) 1997-09-22 2009-07-29 独立行政法人理化学研究所 Ｄｎａの単離方法
DE19746874A1 (de) 1997-10-23 1999-04-29 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
EP1036082B1 (de) * 1997-12-06 2002-05-29 DNA Research Innovations Limited Isolierung von nukleinsäuren
US6194562B1 (en) 1998-04-22 2001-02-27 Promega Corporation Endotoxin reduction in nucleic acid purification
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
US6534262B1 (en) 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
EP1147226B1 (de) 1999-01-27 2013-01-23 Folim G. Halaka Materialien und verfahren zur reinigung von polyelektrolyten
DE19907023A1 (de) 1999-02-19 2000-08-24 Bayer Ag Verfahren zur Isolierung von Nucleinsäuren
CA2270106C (en) 1999-04-23 2006-03-14 Yousef Haj-Ahmad Nucleic acid purification and process
WO2000070040A1 (en) 1999-05-14 2000-11-23 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
AU2001275995A1 (en) 2000-07-31 2002-02-13 Applera Corporation Apparatus and method for specific release of captured extension products
WO2002016580A2 (en) 2000-08-24 2002-02-28 Bio-Rad Laboratories, Inc. Flow-through system for lysate clarification and nucleic acid binding in a single step

Also Published As

Publication number Publication date
JP4868697B2 (ja) 2012-02-01
DK1345952T3 (da) 2005-03-21
EP1473299A2 (de) 2004-11-03
US20010018513A1 (en) 2001-08-30
DE60107468T2 (de) 2005-12-29
CA2432075A1 (en) 2002-06-20
EP1345952B1 (de) 2004-11-24
JP2004521881A (ja) 2004-07-22
ES2233560T3 (es) 2005-06-16
ATE283275T1 (de) 2004-12-15
US6914137B2 (en) 2005-07-05
EP1473299A3 (de) 2006-08-16
US20030054395A1 (en) 2003-03-20
WO2002048164A3 (en) 2002-10-17
AU2002216203A1 (en) 2002-06-24
US20120196944A1 (en) 2012-08-02
US20120197009A1 (en) 2012-08-02
US20080305528A1 (en) 2008-12-11
EP1345952A2 (de) 2003-09-24
WO2002048164A2 (en) 2002-06-20
US20030008320A1 (en) 2003-01-09
US20070231892A1 (en) 2007-10-04
US20130338245A1 (en) 2013-12-19

Similar Documents

Publication Publication Date Title
DE60107468D1 (de) Nucleinsäure-isolation
DE69925856D1 (de) Verfahren zur klonierung von schweinen
DE60238801D1 (de) Zelltrennungszusammensetzungen und -verfahren
ES2162875T3 (es) Metodo para la supresion de la inhibicion de reacciones inducidas por enzimas mediante detergentes ionicos.
ATE513039T1 (de) Nukleinsäureisolationsmethode und entsprechender reagentiensatz dafür
CA2337762A1 (en) MASS MARKERS FOR NUCLEIC ACIDS BINDING TO METAL IONS
DK1036082T3 (da) Isolering af nucleinsyrer
EP0875584A3 (de) Nukleinsäuretestverfahren
DE69802191T2 (de) Festphase nukleinsäure-isolierung
DE69524560T2 (de) Verfahren zum Einfangen und selektiven Freisetzen von Nukleinsäure
ATE335815T1 (de) Isolierung von nukleinsäuren
EP0847452A4 (de) Fluoreszenz resonanz energietransfer nachweisverfahren für die erkennung von hiv-1 hüllenglykoprotein zellen
WO2002010337A3 (en) Method of cloning porcine animals
EP1310793A4 (de) Substrataktivierungskit und verfahren zum nachweis von dna oder so ähnlich
EP1522898A3 (de) Nachfüllösung für Entwickler
BR9910367A (pt) Processo para extrair carotenos de materiais contendo caroteno
CA2092917A1 (en) Hair analysis method
CA2339821A1 (en) Enzymatic measurement of mycophenolic acid
ES2174778T3 (es) Procedimiento de crioconservacion de esperma de pez cebra.
CA2199835A1 (en) Method for preparing nucleic acids for analysis and kits useful therefor
ATE454471T1 (de) Neuartige pufferformulierungen zur isolierung, reinigung und rückgewinnung lang- und kurzkettiger nukleinsäuren
DE50102470D1 (de) Verwendung von quervernetztem polyvinylpyrrolidon als sprengmittel in kompakten, teilchenförmigen wasch- und reinigungsmitteln
DE50212981D1 (de) Immunmodulierende wirkstoffe aus parasitären würmern und verfahren zu deren isolierung
ES2179214T3 (es) Metodos de captura y procedimientos de ensayos.
WO2002056003A3 (en) Method and formulations for the separation of biological macromolecules

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: INVITROGEN CORP.(N.D.GES.D.STAATES DELAWARE), CARL