DE60126786D1 - Verfahren zur ortspezifischen massenmutagenese - Google Patents

Verfahren zur ortspezifischen massenmutagenese

Info

Publication number
DE60126786D1
DE60126786D1 DE60126786T DE60126786T DE60126786D1 DE 60126786 D1 DE60126786 D1 DE 60126786D1 DE 60126786 T DE60126786 T DE 60126786T DE 60126786 T DE60126786 T DE 60126786T DE 60126786 D1 DE60126786 D1 DE 60126786D1
Authority
DE
Germany
Prior art keywords
mutagenesis
site
specific mass
concerns
directed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60126786T
Other languages
English (en)
Other versions
DE60126786T2 (de
Inventor
Marc Delcourt
Stephane Blesa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomethodes SA
Original Assignee
Biomethodes SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomethodes SA filed Critical Biomethodes SA
Application granted granted Critical
Publication of DE60126786D1 publication Critical patent/DE60126786D1/de
Publication of DE60126786T2 publication Critical patent/DE60126786T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
DE60126786T 2000-08-25 2001-08-24 Verfahren zur ortspezifischen massenmutagenese Expired - Lifetime DE60126786T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0010962A FR2813314B1 (fr) 2000-08-25 2000-08-25 Procede de mutagenese dirigee massive
FR0010962 2000-08-25
PCT/FR2001/002666 WO2002016606A2 (fr) 2000-08-25 2001-08-24 Procede de mutagenese dirigee massive

Publications (2)

Publication Number Publication Date
DE60126786D1 true DE60126786D1 (de) 2007-04-05
DE60126786T2 DE60126786T2 (de) 2007-11-08

Family

ID=8853747

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60126786T Expired - Lifetime DE60126786T2 (de) 2000-08-25 2001-08-24 Verfahren zur ortspezifischen massenmutagenese

Country Status (11)

Country Link
US (1) US7202086B2 (de)
EP (1) EP1311670B1 (de)
JP (1) JP5068917B2 (de)
AT (1) ATE354653T1 (de)
AU (1) AU2001286009A1 (de)
CA (1) CA2420721C (de)
DE (1) DE60126786T2 (de)
DK (1) DK1311670T3 (de)
ES (1) ES2282285T3 (de)
FR (1) FR2813314B1 (de)
WO (1) WO2002016606A2 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
US7582423B2 (en) 2001-02-02 2009-09-01 Novici Biotech Llc Population of polynucleotide sequence variants
US20040142433A1 (en) * 2001-02-02 2004-07-22 Padgett Hal S. Polynucleotide sequence variants
US20050153283A1 (en) * 2002-08-08 2005-07-14 Padgett Hal S. Method of increasing complementarity in a heteroduplex
US7838219B2 (en) * 2001-02-02 2010-11-23 Novici Biotech Llc Method of increasing complementarity in a heteroduplex
CA2436214C (en) * 2001-02-02 2012-12-04 Large Scale Biology Corporation A method of increasing complementarity in a heteroduplex polynucleotide
FR2823219B1 (fr) * 2001-04-10 2003-07-04 Pasteur Institut Mutants de la desoxycytidine kinase possedant une activite enzymatique elargie
US7647184B2 (en) 2001-08-27 2010-01-12 Hanall Pharmaceuticals, Co. Ltd High throughput directed evolution by rational mutagenesis
US7078211B2 (en) 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
AU2003263552A1 (en) 2002-09-09 2004-03-29 Nautilus Biotech Rational evolution of cytokines for higher stability, the cytokines and encoding nucleic acid molecules
DE602004020570D1 (de) * 2003-12-18 2009-05-28 Biomethodes Verfahren zur ortspezifischen Massenmutagenese
FR2883885B1 (fr) * 2005-04-05 2010-12-10 Biomethodes Recombinaison in vitro circulaire
FR2886943B1 (fr) 2005-06-10 2007-09-07 Biomethodes Sa Methode de selection de proteines stables dans des conditions physico-chimiques non standard
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
FR2897068B1 (fr) * 2006-02-03 2008-04-18 Biomethodes Sa Kanamycine kinase ii amelioree et utilisations de celle-ci
CA2644127A1 (fr) * 2006-03-08 2007-09-13 Biomethodes Variants ameliores de l'interferon-gamma humain (ifny)
NZ582876A (en) 2007-07-31 2012-07-27 Bp Corp North America Inc Tailored multi-site combinatorial assembly of polynucleotides
CA2894225A1 (fr) 2012-12-17 2014-06-26 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Utilisation d'anticorps monoclonaux pour le traitement de l'inflammation et d'infections bacteriennes
US9637728B2 (en) 2013-07-26 2017-05-02 Biomethodes Variant Trichoderma reesei endoglucanases
CN111492063A (zh) * 2017-12-20 2020-08-04 巴斯夫欧洲公司 基因位点饱和诱变(gssm)方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4960707A (en) * 1987-08-17 1990-10-02 Associated Universities, Inc. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae
EP0527809B1 (de) * 1990-04-05 1995-08-16 CREA, Roberto "walk-through"-mutagenese
WO1993020096A1 (en) * 1991-05-08 1993-10-14 Stratagene Oligonucleotide libraries useful for producing primers
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
DE69934088T2 (de) * 1998-01-09 2007-06-21 University Of Utah Research Foundation, Salt Lake City Verfahren zur in vitro amplifikation zirkulärer dna
US6368861B1 (en) * 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination

Also Published As

Publication number Publication date
US20040048268A1 (en) 2004-03-11
ES2282285T3 (es) 2007-10-16
WO2002016606A2 (fr) 2002-02-28
AU2001286009A1 (en) 2002-03-04
FR2813314B1 (fr) 2004-05-07
WO2002016606A3 (fr) 2002-06-27
FR2813314A1 (fr) 2002-03-01
CA2420721A1 (fr) 2002-02-28
ATE354653T1 (de) 2007-03-15
JP5068917B2 (ja) 2012-11-07
JP2004507243A (ja) 2004-03-11
DK1311670T3 (da) 2007-06-18
CA2420721C (fr) 2013-11-12
EP1311670B1 (de) 2007-02-21
EP1311670A2 (de) 2003-05-21
DE60126786T2 (de) 2007-11-08
US7202086B2 (en) 2007-04-10

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