DE60126786D1 - Verfahren zur ortspezifischen massenmutagenese - Google Patents

Verfahren zur ortspezifischen massenmutagenese

Info

Publication number
DE60126786D1
DE60126786D1 DE60126786T DE60126786T DE60126786D1 DE 60126786 D1 DE60126786 D1 DE 60126786D1 DE 60126786 T DE60126786 T DE 60126786T DE 60126786 T DE60126786 T DE 60126786T DE 60126786 D1 DE60126786 D1 DE 60126786D1
Authority
DE
Germany
Prior art keywords
mutagenesis
site
specific mass
concerns
directed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60126786T
Other languages
English (en)
Other versions
DE60126786T2 (de
Inventor
Marc Delcourt
Stephane Blesa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomethodes SA
Original Assignee
Biomethodes SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomethodes SA filed Critical Biomethodes SA
Application granted granted Critical
Publication of DE60126786D1 publication Critical patent/DE60126786D1/de
Publication of DE60126786T2 publication Critical patent/DE60126786T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
DE60126786T 2000-08-25 2001-08-24 Verfahren zur ortspezifischen massenmutagenese Expired - Lifetime DE60126786T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0010962 2000-08-25
FR0010962A FR2813314B1 (fr) 2000-08-25 2000-08-25 Procede de mutagenese dirigee massive
PCT/FR2001/002666 WO2002016606A2 (fr) 2000-08-25 2001-08-24 Procede de mutagenese dirigee massive

Publications (2)

Publication Number Publication Date
DE60126786D1 true DE60126786D1 (de) 2007-04-05
DE60126786T2 DE60126786T2 (de) 2007-11-08

Family

ID=8853747

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60126786T Expired - Lifetime DE60126786T2 (de) 2000-08-25 2001-08-24 Verfahren zur ortspezifischen massenmutagenese

Country Status (11)

Country Link
US (1) US7202086B2 (de)
EP (1) EP1311670B1 (de)
JP (1) JP5068917B2 (de)
AT (1) ATE354653T1 (de)
AU (1) AU2001286009A1 (de)
CA (1) CA2420721C (de)
DE (1) DE60126786T2 (de)
DK (1) DK1311670T3 (de)
ES (1) ES2282285T3 (de)
FR (1) FR2813314B1 (de)
WO (1) WO2002016606A2 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
US20050153283A1 (en) * 2002-08-08 2005-07-14 Padgett Hal S. Method of increasing complementarity in a heteroduplex
US20040142433A1 (en) * 2001-02-02 2004-07-22 Padgett Hal S. Polynucleotide sequence variants
US7582423B2 (en) 2001-02-02 2009-09-01 Novici Biotech Llc Population of polynucleotide sequence variants
US7838219B2 (en) * 2001-02-02 2010-11-23 Novici Biotech Llc Method of increasing complementarity in a heteroduplex
JP4689940B2 (ja) * 2001-02-02 2011-06-01 ノビシ バイオテック，リミティド ライアビリティ カンパニー ヘテロ二本鎖の相補性を増大させる方法
FR2823219B1 (fr) * 2001-04-10 2003-07-04 Pasteur Institut Mutants de la desoxycytidine kinase possedant une activite enzymatique elargie
US7647184B2 (en) 2001-08-27 2010-01-12 Hanall Pharmaceuticals, Co. Ltd High throughput directed evolution by rational mutagenesis
US7078211B2 (en) * 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
CA2498319A1 (en) 2002-09-09 2004-03-18 Nautilus Biotech Rational evolution of cytokines for higher stability, the cytokines and encoding nucleic acid molecules
EP1544296B8 (de) * 2003-12-18 2009-08-05 Biomethodes Verfahren zur ortspezifischen Massenmutagenese
FR2883885B1 (fr) * 2005-04-05 2010-12-10 Biomethodes Recombinaison in vitro circulaire
FR2886943B1 (fr) 2005-06-10 2007-09-07 Biomethodes Sa Methode de selection de proteines stables dans des conditions physico-chimiques non standard
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
FR2897068B1 (fr) * 2006-02-03 2008-04-18 Biomethodes Sa Kanamycine kinase ii amelioree et utilisations de celle-ci
WO2007101949A2 (fr) * 2006-03-08 2007-09-13 Biomethodes Variants de l'interferon- gamma humain (infgamma)
BRPI0815046A2 (pt) 2007-07-31 2014-10-14 Verenium Corp Personalização de montagem combinatória de múltiplos locais
US20160185847A1 (en) 2012-12-17 2016-06-30 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Use of monoclonal antibodies for the treatment of inflammation and bacterial infections
EP3024930A2 (de) 2013-07-26 2016-06-01 Biomethodes Neuartige variante trichoderma-reesei-endoglucanasen
CN111492063A (zh) * 2017-12-20 2020-08-04 巴斯夫欧洲公司 基因位点饱和诱变(gssm)方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4960707A (en) * 1987-08-17 1990-10-02 Associated Universities, Inc. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae
CA2079802C (en) * 1990-04-05 2001-09-18 Roberto Crea Walk-through mutagenesis
WO1993020096A1 (en) * 1991-05-08 1993-10-14 Stratagene Oligonucleotide libraries useful for producing primers
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
CA2317896C (en) * 1998-01-09 2004-08-10 University Of Utah Research Foundation Method for in vitro amplification of circular dna
US6368861B1 (en) * 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination

Also Published As

Publication number Publication date
JP2004507243A (ja) 2004-03-11
DK1311670T3 (da) 2007-06-18
CA2420721A1 (fr) 2002-02-28
WO2002016606A3 (fr) 2002-06-27
DE60126786T2 (de) 2007-11-08
FR2813314B1 (fr) 2004-05-07
EP1311670B1 (de) 2007-02-21
AU2001286009A1 (en) 2002-03-04
ES2282285T3 (es) 2007-10-16
WO2002016606A2 (fr) 2002-02-28
FR2813314A1 (fr) 2002-03-01
ATE354653T1 (de) 2007-03-15
CA2420721C (fr) 2013-11-12
EP1311670A2 (de) 2003-05-21
US20040048268A1 (en) 2004-03-11
JP5068917B2 (ja) 2012-11-07
US7202086B2 (en) 2007-04-10

Similar Documents

Publication Publication Date Title
DE60126786D1 (de) Verfahren zur ortspezifischen massenmutagenese
AU2001267232A1 (en) Method and apparatus for improved process control in combustion applications
ATE426021T1 (de) Verfahren zur herstellung von biopterinen
HK1071698A1 (en) Autologous t-cell vaccines materials and method
DE60126933D1 (de) Verfahren zur fermentativen herstellung von l-threonine
GB2380201B (en) Method of fuel production from fischer-tropsch process
FR2812661B1 (fr) Acier haute proprete et son procede de production
DE502005007004D1 (de) Hartkern-mantelgeschoss mit leuchtsatz und verfahren zu seiner herstellung
HK1087417A1 (en) Method and apparatus for continuous large-scale radiolabeling of proteins
MY141859A (en) Method for producing gamma-carboxylated proteins
HK1056292A1 (en) Method of culturing zooplankton, apparatus for culturing by the same, and culture obtained by the same.
ATE361334T1 (de) Verfahren zur herstellung von hochreinem polycarbonat
EP1662002A4 (de) Verfahren zur einführung eines gens in pflanzenmaterial
IL164625A (en) Method for producing a plurality of monoclonal antibodies and method for identifying a plurality of monoclonal antibodies
BR0013857B1 (pt) processo de produÇço de exopolissacarÍdeos.
HK1041250A1 (zh) 生物反應物載體，產生該載體的過程及其使用方法
AU2003247599A1 (en) Methods of modulating cytokinin related processes in a plant using b3 domain proteins
ATE277919T1 (de) Verfahren zur herstellung von baccatin iii- derivativen
WO2002061611A3 (de) Verfahren zur darstellung von einem internetobjekt
DE50213120D1 (de) Mikrobiologisches verfahren zur herstellung von amiden
ATE549405T1 (de) Bakterienmutanten zur l-lysinherstellung
ATE522604T1 (de) Verfahren zur herstellung von proteinen durch elektropulsierung
FR2792337B1 (fr) Heteropolysaccharide produit par un pseudomonas sp
EP1295942A3 (de) Methode zur Bearbeitung einer Bibliothek unter Verwendung von Ligation-Inhibierung
DE50203529D1 (de) Verfahren zur herstellung von desclarithromycin, sowie zwischenprodukte

Legal Events

Date Code Title Description
8364 No opposition during term of opposition