DE60140487D1 - Asynchrones pcr priming - Google Patents

Asynchrones pcr priming

Info

Publication number
DE60140487D1
DE60140487D1 DE60140487T DE60140487T DE60140487D1 DE 60140487 D1 DE60140487 D1 DE 60140487D1 DE 60140487 T DE60140487 T DE 60140487T DE 60140487 T DE60140487 T DE 60140487T DE 60140487 D1 DE60140487 D1 DE 60140487D1
Authority
DE
Germany
Prior art keywords
probe
reporter dye
stranded
annealed
fluorescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60140487T
Other languages
English (en)
Inventor
Caifu Chen
Michael Egholm
Lawrence Haff
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Applied Biosystems LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Biosystems LLC filed Critical Applied Biosystems LLC
Application granted granted Critical
Publication of DE60140487D1 publication Critical patent/DE60140487D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
DE60140487T 2000-06-06 2001-06-06 Asynchrones pcr priming Expired - Lifetime DE60140487D1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20988300P 2000-06-06 2000-06-06
US09/875,211 US6887664B2 (en) 2000-06-06 2001-06-05 Asynchronous primed PCR
PCT/US2001/018464 WO2001094638A2 (en) 2000-06-06 2001-06-06 Asynchronous primed pcr

Publications (1)

Publication Number Publication Date
DE60140487D1 true DE60140487D1 (en) 2009-12-24

Family

ID=26904613

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60140487T Expired - Lifetime DE60140487D1 (en) 2000-06-06 2001-06-06 Asynchrones pcr priming

Country Status (8)

Country Link
US (2) US6887664B2 (de)
EP (3) EP2256213A1 (de)
JP (1) JP2004509613A (de)
AT (1) ATE448325T1 (de)
AU (1) AU6823501A (de)
CA (1) CA2412413A1 (de)
DE (1) DE60140487D1 (de)
WO (1) WO2001094638A2 (de)

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Also Published As

Publication number Publication date
EP2256213A1 (de) 2010-12-01
ATE448325T1 (de) 2009-11-15
US20030207266A1 (en) 2003-11-06
WO2001094638A3 (en) 2003-07-10
US20050130178A1 (en) 2005-06-16
US6887664B2 (en) 2005-05-03
EP1368489A2 (de) 2003-12-10
WO2001094638A8 (en) 2002-04-11
CA2412413A1 (en) 2001-12-13
EP1368489B1 (de) 2009-11-11
EP2316975A1 (de) 2011-05-04
JP2004509613A (ja) 2004-04-02
AU6823501A (en) 2001-12-17
WO2001094638A2 (en) 2001-12-13

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