DE602004022282D1 - Verfahren zur amplifikation von nukleinsäuresequenzen mittels gestaffelter ligation - Google Patents

Verfahren zur amplifikation von nukleinsäuresequenzen mittels gestaffelter ligation

Info

Publication number
DE602004022282D1
DE602004022282D1 DE602004022282T DE602004022282T DE602004022282D1 DE 602004022282 D1 DE602004022282 D1 DE 602004022282D1 DE 602004022282 T DE602004022282 T DE 602004022282T DE 602004022282 T DE602004022282 T DE 602004022282T DE 602004022282 D1 DE602004022282 D1 DE 602004022282D1
Authority
DE
Germany
Prior art keywords
sense
double stranded
rna polymerase
rna
oligodeoxynucleotide tail
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602004022282T
Other languages
English (en)
Inventor
Robert C Getts
Jaime Schwalm
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genisphere LLC
Original Assignee
Genisphere Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genisphere Inc filed Critical Genisphere Inc
Publication of DE602004022282D1 publication Critical patent/DE602004022282D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
DE602004022282T 2003-05-09 2004-05-07 Verfahren zur amplifikation von nukleinsäuresequenzen mittels gestaffelter ligation Active DE602004022282D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46938303P 2003-05-09 2003-05-09
PCT/US2004/014325 WO2004101749A2 (en) 2003-05-09 2004-05-07 Methods for amplification of nucleic acid sequences using staggered ligation

Publications (1)

Publication Number Publication Date
DE602004022282D1 true DE602004022282D1 (de) 2009-09-10

Family

ID=33452281

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004022282T Active DE602004022282D1 (de) 2003-05-09 2004-05-07 Verfahren zur amplifikation von nukleinsäuresequenzen mittels gestaffelter ligation

Country Status (7)

Country Link
US (1) US7494789B2 (de)
EP (1) EP1623044B1 (de)
JP (1) JP4599483B2 (de)
AT (1) ATE437964T1 (de)
AU (1) AU2004239283B2 (de)
DE (1) DE602004022282D1 (de)
WO (1) WO2004101749A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE602005017252D1 (de) * 2004-04-01 2009-12-03 Genisphere Inc Verfahren zur amplifikation von nukleinsäuren mittels promotor-templates
US7550264B2 (en) 2005-06-10 2009-06-23 Datascope Investment Corporation Methods and kits for sense RNA synthesis
US20070105124A1 (en) 2005-11-08 2007-05-10 Getts Robert C Methods and kits for nucleic acid amplification
US7998423B2 (en) 2007-02-27 2011-08-16 Basf Corporation SCR on low thermal mass filter substrates
AU2009321519A1 (en) * 2008-11-03 2011-06-30 Alchemy Biosciences Pty Ltd Method of cloning DNA
JP6509723B2 (ja) * 2012-03-13 2019-05-08 スウィフト バイオサイエンシーズ， インコーポレイテッド 核酸ポリメラーゼによる基質ポリヌクレオチドの大きさ制御されたホモポリマーテーリングのための方法および組成物
WO2014108850A2 (en) * 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
GB201903391D0 (en) * 2019-03-12 2019-04-24 Cancer Research Tech Ltd Nucleic acid amplification methods

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5215899A (en) 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription
US5194370A (en) * 1990-05-16 1993-03-16 Life Technologies, Inc. Promoter ligation activated transcription amplification of nucleic acid sequences
PT1056884E (pt) * 1998-02-27 2002-06-28 Pamgene Bv Processo para a amplificacao nao especifica de acido nucleico
US20020102589A1 (en) 2000-10-13 2002-08-01 Ryoiti Kiyama Microarrays and methods for evaluating activity of compounds having estrogen-like activity
JP2002360249A (ja) * 2000-10-13 2002-12-17 National Institute Of Advanced Industrial & Technology マイクロアレイ及びエストロゲン類似活性化合物の活性評価方法
US20030104432A1 (en) * 2001-07-27 2003-06-05 The Regents Of The University Of California Methods of amplifying sense strand RNA
GB0123401D0 (en) * 2001-09-28 2001-11-21 Novartis Forschungsstiftung Methods of inducing gene expression
DE602005017252D1 (de) 2004-04-01 2009-12-03 Genisphere Inc Verfahren zur amplifikation von nukleinsäuren mittels promotor-templates

Also Published As

Publication number Publication date
ATE437964T1 (de) 2009-08-15
EP1623044A4 (de) 2007-11-07
AU2004239283A1 (en) 2004-11-25
WO2004101749A3 (en) 2005-02-10
JP2007515938A (ja) 2007-06-21
US7494789B2 (en) 2009-02-24
AU2004239283B2 (en) 2010-01-28
WO2004101749A2 (en) 2004-11-25
EP1623044A2 (de) 2006-02-08
JP4599483B2 (ja) 2010-12-15
US20070072182A1 (en) 2007-03-29
EP1623044B1 (de) 2009-07-29

Similar Documents

Publication Publication Date Title
ATE418609T1 (de) Verfahren für die kontinuierliche zielgerichtete evolution von proteinen in vitro
WO2006081222A3 (en) Isothermal dna amplification
PT1218542E (pt) Metodos e composicoes para amplificacao isotermica linear de sequencias polinucleotidicas
JP2017537626A5 (de)
ATE328075T1 (de) Verfahren zur hemmung der expression eine zielgens
DE69827745D1 (de) Kovalente bindung von dna zu rna strängen katalysiert durch vaccinia topoisomerase
AU2008339215A8 (en) Novel siRNA structure for minimizing off-target effects and relaxing saturation of RNAi machinery and the use thereof
DE60227525D1 (de) Verfahren zur Herstellung von Nukleinsäuremolekülen
CA2610929A1 (en) Methods and kits for sense rna synthesis
WO2004007718A3 (en) Rna-interference by single-stranded rna molecules
ATE550427T1 (de) Modifikationen von rna, die zu einer erhöhten transkriptstabilität und translationseffizienz führen
ATE411400T1 (de) Verfahren zur verwendung von riboprimern zur strangverdrängungsreplikation von zielsequenzen
DE602004022282D1 (de) Verfahren zur amplifikation von nukleinsäuresequenzen mittels gestaffelter ligation
MX2007004686A (es) Moleculas de arn de multidominio que comprenden al menos un aptamero para suministrar arn de doble hebra a organismos nocivos.
WO2004065600A3 (en) Rna interference by palindromic or modified rna molecules
RU2020130258A (ru) Способ получения шпилечной одноцепочечной молекулы рнк
WO2003102232A3 (de) Vermehrung von ribonukleinsäuren
ATE220427T1 (de) Verfahren zum nachweis von nukleinsäure- zielsequenzen mittels in vitro transkription von einem rna-promoter
WO2005054270A3 (en) Identification and design of sirna molecules
WO2005042705A3 (en) Short interfering rna and micro-rna compounds and methods of designing, making and using the same
ATE539167T1 (de) Verfahren zur amplifikation von rna-sequenzen
AU2003288105A1 (en) Microarray-based method for amplifying and detecting nucleic acids during a continuous process
ATE427996T1 (de) Verfahren zur herstellung von (s)-butan-2-ol
JP2007515938A5 (de)
DE69902799T2 (de) Von kreisförmiger Matrize abhängige Kettenreaktion

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: GENISPHERE, LLC, HATFIELD, PA., US

8328 Change in the person/name/address of the agent

Representative=s name: PETERS, H., DIPL.-BIOCHEM., PAT.-ASS., 80335 MUENC