DE602004027310D1 - Herstellungsverfahren zur produktion aktiver und löslicher proteine in prokaryonten und polycistronische vektoren dafür - Google Patents

Herstellungsverfahren zur produktion aktiver und löslicher proteine in prokaryonten und polycistronische vektoren dafür

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Publication number
DE602004027310D1
DE602004027310D1 DE602004027310T DE602004027310T DE602004027310D1 DE 602004027310 D1 DE602004027310 D1 DE 602004027310D1 DE 602004027310 T DE602004027310 T DE 602004027310T DE 602004027310 T DE602004027310 T DE 602004027310T DE 602004027310 D1 DE602004027310 D1 DE 602004027310D1
Authority
DE
Germany
Prior art keywords
prokaryons
soluble proteins
manufacturing active
polycistronic vectors
vectors therefor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602004027310T
Other languages
English (en)
Inventor
Bong Yong Lee
S K Park
Shin Hye Park
Oh Byung Kwon
Chae Ha Yoon
Young Ju Kim
Song Young Kim
Kyung Hyun Min
Yoon Seok Lee
Tae Hee Lee
Tai Young Koo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Daewoong Co Ltd
Original Assignee
Daewoong Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Daewoong Co Ltd filed Critical Daewoong Co Ltd
Publication of DE602004027310D1 publication Critical patent/DE602004027310D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
DE602004027310T 2004-05-06 2004-06-11 Herstellungsverfahren zur produktion aktiver und löslicher proteine in prokaryonten und polycistronische vektoren dafür Active DE602004027310D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020040031977A KR100484653B1 (ko) 2004-05-06 2004-05-06 원핵세포에서 활성형의 가용성 단백질을 제조하는 방법 및 이를 위한 폴리시스트론 벡터
PCT/KR2004/001393 WO2005108585A1 (en) 2004-05-06 2004-06-11 Preparation method for the production of active and soluble proteins in prokaryotes and polycistronic vectors therefor

Publications (1)

Publication Number Publication Date
DE602004027310D1 true DE602004027310D1 (de) 2010-07-01

Family

ID=35320239

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004027310T Active DE602004027310D1 (de) 2004-05-06 2004-06-11 Herstellungsverfahren zur produktion aktiver und löslicher proteine in prokaryonten und polycistronische vektoren dafür

Country Status (8)

Country Link
US (1) US7811786B1 (de)
EP (1) EP1747274B9 (de)
JP (1) JP4510884B2 (de)
KR (1) KR100484653B1 (de)
CN (1) CN101023174B (de)
AT (1) ATE468398T1 (de)
DE (1) DE602004027310D1 (de)
WO (1) WO2005108585A1 (de)

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EP2468768A3 (de) 2005-07-21 2012-10-31 Abbott Laboratories Mehrfache Genexpression mit sORF-Konstrukten und Verfahren mit Polyproteinen, Pro-Proteinen und Proteolyse
EP3399025A1 (de) 2007-03-23 2018-11-07 Wisconsin Alumini Research Foundation Neuprogrammierung von körperzellen
DK2297307T3 (en) 2008-06-04 2016-07-25 Cellular Dynamics Int Inc PROCEDURES FOR THE MANUFACTURE OF IPS CELLS USING NON-VIRAL METHODS
EP3330371A1 (de) 2008-08-12 2018-06-06 Cellular Dynamics International, Inc. Verfahren zur herstellung von ips-zellen
WO2011060488A1 (en) * 2009-11-18 2011-05-26 Murray Goulburn Co-Operative Co. Limited Recombinant microorganisms
GB201018664D0 (en) * 2010-11-05 2010-12-22 Msd Biolog Uk Ltd Expression process
CN102329811B (zh) * 2011-09-15 2014-08-13 生工生物工程（上海）股份有限公司 一种氨苄青霉素抗性质粒载体及其制备与应用
HUP1200171A1 (hu) * 2012-03-19 2013-09-30 Richter Gedeon Nyrt Módszerek polipeptidek elõállítására
KR101477235B1 (ko) 2012-09-28 2015-01-02 선문대학교 산학협력단 합성생물학에서 사용되는 멀티-모노시스트로닉 벡터의 제작과 응용
WO2014082080A2 (en) * 2012-11-26 2014-05-30 Callidus Biopharma, Inc. Methods for coupling targeting peptides onto recombinant lysosomal enzymes for improved treatments of lysosomal storage diseases
JP6803328B2 (ja) 2014-08-28 2021-01-06 シンセティック バイオロジクス，インコーポレイテッド 大腸菌ベースでのベータラクタマーゼ生産
CN104946647A (zh) * 2015-06-08 2015-09-30 南京理工大学 一种莱茵衣藻基因间表达处理元件
CN105420263B (zh) * 2015-12-16 2019-07-26 温州医科大学 重组人成纤维细胞生长因子-17的生产方法
CN106434733B (zh) * 2016-08-01 2019-09-27 江南大学 一种适用于谷氨酸棒杆菌的表达载体及其应用
JP7083124B2 (ja) 2017-04-25 2022-06-10 国立大学法人弘前大学 ムコン酸産生形質転換微生物及びその利用
EP3643781A4 (de) * 2017-06-23 2021-07-14 Zhuhai Essex Bio-pharmaceutical Co., Ltd. Verfahren zur herstellung eines löslichen rekombinanten humanen basischen fibroblasten-wachstumsfaktors (rh-bfgf)
KR102480402B1 (ko) * 2017-06-23 2022-12-22 주하이 에섹스 바이오-파머슈티컬 코., 엘티디. 재조합 인간-염기성 섬유아세포 성장 인자 (rh-bFGF) 및 rh-bFGF를 포함하는 약학적 조성물
KR102440312B1 (ko) * 2020-08-28 2022-09-05 한국해양과학기술원 온도안정성을 향상시킨 fgf7 폴리펩타이드 및 그 용도

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Publication number Priority date Publication date Assignee Title
IL80511A0 (en) * 1985-11-05 1987-02-27 Genetics Inst Multiply amplifiable vectors for high level expression of exogenous dna
JP2570651B2 (ja) * 1986-10-09 1997-01-08 藤沢薬品工業株式会社 Ｍｅｔ−インスリン様成長因子Ｉの製造方法
US5648254A (en) 1988-01-15 1997-07-15 Zymogenetics, Inc. Co-expression in eukaryotic cells
AU2660397A (en) 1996-04-05 1997-10-29 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells
KR100203919B1 (ko) 1996-10-04 1999-06-15 신동권 수용성 단백질을 생산하는 새로운 발현 플라스미드
KR100254063B1 (ko) 1997-09-30 2000-04-15 신동권 재조합대장균을이용한재조합단백질의제조방법
ATE463580T1 (de) 2000-12-26 2010-04-15 Sekisui Chemical Co Ltd Verfahren zur herstellung von rekombinantem protein und fusionsprotein
SI1368483T1 (sl) 2001-03-09 2013-01-31 Genentech, Inc. Postopek za pripravo polipeptidov
KR20040007892A (ko) 2002-07-11 2004-01-28 주식회사 바이오프로젠 재조합 인간 훼리틴 단백질 및 그 제조방법

Also Published As

Publication number Publication date
EP1747274B9 (de) 2011-02-02
JP2007535923A (ja) 2007-12-13
EP1747274B1 (de) 2010-05-19
JP4510884B2 (ja) 2010-07-28
US7811786B1 (en) 2010-10-12
KR100484653B1 (ko) 2005-04-20
ATE468398T1 (de) 2010-06-15
CN101023174A (zh) 2007-08-22
CN101023174B (zh) 2011-03-02
EP1747274A4 (de) 2007-07-11
EP1747274A1 (de) 2007-01-31
WO2005108585A1 (en) 2005-11-17

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