DE602006017405D1 - Verfahren zur in vitro-rekombination - Google Patents
Verfahren zur in vitro-rekombinationInfo
- Publication number
- DE602006017405D1 DE602006017405D1 DE602006017405T DE602006017405T DE602006017405D1 DE 602006017405 D1 DE602006017405 D1 DE 602006017405D1 DE 602006017405 T DE602006017405 T DE 602006017405T DE 602006017405 T DE602006017405 T DE 602006017405T DE 602006017405 D1 DE602006017405 D1 DE 602006017405D1
- Authority
- DE
- Germany
- Prior art keywords
- molecules
- dna
- region
- stranded
- substantially purified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US70717705P | 2005-08-11 | 2005-08-11 | |
| US80040006P | 2006-05-16 | 2006-05-16 | |
| PCT/US2006/031214 WO2007032837A2 (en) | 2005-08-11 | 2006-08-11 | Method for in vitro recombination |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE602006017405D1 true DE602006017405D1 (de) | 2010-11-18 |
Family
ID=37734039
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE602006017405T Active DE602006017405D1 (de) | 2005-08-11 | 2006-08-11 | Verfahren zur in vitro-rekombination |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US7776532B2 (de) |
| EP (2) | EP1929012B1 (de) |
| AT (1) | ATE483802T1 (de) |
| CA (1) | CA2618665C (de) |
| DE (1) | DE602006017405D1 (de) |
| DK (2) | DK1929012T3 (de) |
| MY (1) | MY144014A (de) |
| WO (1) | WO2007032837A2 (de) |
Families Citing this family (60)
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| US7723077B2 (en) | 2005-08-11 | 2010-05-25 | Synthetic Genomics, Inc. | In vitro recombination method |
| WO2007028147A2 (en) * | 2005-09-01 | 2007-03-08 | Philadelphia Health & Education Corporation D.B.A. Drexel University College Of Medicin | Identification of a prostatic intraepithelial neoplasia (pin)-specific gene and protein (pin-1) useful as a diagnostic treatment for prostate cancer |
| CA2625262A1 (en) * | 2005-10-13 | 2007-10-04 | Bc Cancer Agency | Modular genomes for synthetic biology and metabolic engineering |
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| WO2008027452A2 (en) * | 2006-08-29 | 2008-03-06 | R. Crea And Company | Self-assembling oligonucleotides and methods |
| CA2701923C (en) * | 2007-10-08 | 2018-07-10 | Synthetic Genomics, Inc. | Assembly of large nucleic acids |
| US9267132B2 (en) | 2007-10-08 | 2016-02-23 | Synthetic Genomics, Inc. | Methods for cloning and manipulating genomes |
| WO2009103027A2 (en) * | 2008-02-15 | 2009-08-20 | Synthetic Genomics, Inc. | Methods for in vitro joining and combinatorial assembly of nucleic acid molecules |
| US10093552B2 (en) | 2008-02-22 | 2018-10-09 | James Weifu Lee | Photovoltaic panel-interfaced solar-greenhouse distillation systems |
| US9259662B2 (en) | 2008-02-22 | 2016-02-16 | James Weifu Lee | Photovoltaic panel-interfaced solar-greenhouse distillation systems |
| ES2612749T3 (es) | 2008-02-23 | 2017-05-18 | James Weifu Lee | Organismos de diseño para la producción fotobiológica de butanol a partir de dióxido de carbono y agua |
| US8986963B2 (en) * | 2008-02-23 | 2015-03-24 | James Weifu Lee | Designer calvin-cycle-channeled production of butanol and related higher alcohols |
| SG190572A1 (en) | 2008-04-29 | 2013-06-28 | Abbott Lab | Dual variable domain immunoglobulins and uses thereof |
| RU2010153580A (ru) | 2008-06-03 | 2012-07-20 | Эбботт Лэборетриз (Us) | Иммуноглобулины с двумя вариабельными доменами и их применение |
| CN102124112B (zh) * | 2008-09-10 | 2013-03-20 | 金斯瑞公司 | 基于同源重组dna的克隆方法及组合物 |
| WO2010045326A1 (en) * | 2008-10-14 | 2010-04-22 | Barnes Wayne M | In vitro recombination methods using 5' exonuclease |
| CN102282255B (zh) * | 2008-11-19 | 2014-05-07 | 阿迈瑞斯公司 | 多核苷酸装配的组合物和方法 |
| CN105274004B (zh) * | 2009-03-06 | 2019-08-09 | 合成基因组股份有限公司 | 用于克隆和操作基因组的方法 |
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| PE20131412A1 (es) | 2010-08-03 | 2014-01-19 | Abbvie Inc | Inmunoglobulinas con dominio variable dual y usos de las mismas |
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| JP6004423B2 (ja) * | 2012-05-07 | 2016-10-05 | 国立研究開発法人産業技術総合研究所 | 遺伝子連結法およびそれを用いた単鎖抗体作製方法 |
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| KR101398764B1 (ko) | 2013-08-29 | 2014-05-27 | 강릉원주대학교산학협력단 | 입자의 이동에 의해 분석물질을 검출하는 장치 및 방법 |
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| US9981239B2 (en) | 2015-04-21 | 2018-05-29 | Twist Bioscience Corporation | Devices and methods for oligonucleic acid library synthesis |
| AU2016324296A1 (en) | 2015-09-18 | 2018-04-12 | Twist Bioscience Corporation | Oligonucleic acid variant libraries and synthesis thereof |
| US11512347B2 (en) | 2015-09-22 | 2022-11-29 | Twist Bioscience Corporation | Flexible substrates for nucleic acid synthesis |
| CN115920796A (zh) | 2015-12-01 | 2023-04-07 | 特韦斯特生物科学公司 | 功能化表面及其制备 |
| CA3034769A1 (en) | 2016-08-22 | 2018-03-01 | Twist Bioscience Corporation | De novo synthesized nucleic acid libraries |
| WO2018057526A2 (en) | 2016-09-21 | 2018-03-29 | Twist Bioscience Corporation | Nucleic acid based data storage |
| CN106701738B (zh) * | 2016-11-10 | 2020-05-08 | 中国科学院成都生物研究所 | 一种等温解开双链dna及制备单链dna的方法 |
| US10907274B2 (en) | 2016-12-16 | 2021-02-02 | Twist Bioscience Corporation | Variant libraries of the immunological synapse and synthesis thereof |
| CN110892485B (zh) | 2017-02-22 | 2024-03-22 | 特韦斯特生物科学公司 | 基于核酸的数据存储 |
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| EP3595674A4 (de) | 2017-03-15 | 2020-12-16 | Twist Bioscience Corporation | Variante bibliotheken der immunologischen synapse und synthese davon |
| CN106995813B (zh) * | 2017-03-23 | 2020-06-16 | 山东大学 | 基因组大片段直接克隆和dna多分子组装新技术 |
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-
2006
- 2006-08-11 AT AT06836102T patent/ATE483802T1/de not_active IP Right Cessation
- 2006-08-11 EP EP06836102A patent/EP1929012B1/de active Active
- 2006-08-11 DK DK06836102.1T patent/DK1929012T3/da active
- 2006-08-11 US US11/502,624 patent/US7776532B2/en active Active
- 2006-08-11 CA CA2618665A patent/CA2618665C/en active Active
- 2006-08-11 DE DE602006017405T patent/DE602006017405D1/de active Active
- 2006-08-11 WO PCT/US2006/031214 patent/WO2007032837A2/en active Application Filing
- 2006-08-11 DK DK10168787.9T patent/DK2239327T3/en active
- 2006-08-11 MY MYPI20063911A patent/MY144014A/en unknown
- 2006-08-11 EP EP10168787.9A patent/EP2239327B1/de active Active
-
2010
- 2010-06-22 US US12/820,861 patent/US8435736B2/en active Active
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2013
- 2013-04-16 US US13/864,108 patent/US20130295645A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| DK1929012T3 (da) | 2011-01-31 |
| CA2618665C (en) | 2012-11-13 |
| WO2007032837A3 (en) | 2007-05-24 |
| MY144014A (en) | 2011-07-29 |
| EP2239327A2 (de) | 2010-10-13 |
| ATE483802T1 (de) | 2010-10-15 |
| EP2239327A3 (de) | 2012-04-25 |
| US20130295645A1 (en) | 2013-11-07 |
| US20100311126A1 (en) | 2010-12-09 |
| EP2239327B1 (de) | 2015-02-25 |
| EP1929012A2 (de) | 2008-06-11 |
| WO2007032837A2 (en) | 2007-03-22 |
| US20070037196A1 (en) | 2007-02-15 |
| US7776532B2 (en) | 2010-08-17 |
| DK2239327T3 (en) | 2015-05-18 |
| CA2618665A1 (en) | 2007-03-22 |
| US8435736B2 (en) | 2013-05-07 |
| EP1929012B1 (de) | 2010-10-06 |
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