DE60324810D1 - HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN - Google Patents

HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN

Info

Publication number
DE60324810D1
DE60324810D1 DE60324810T DE60324810T DE60324810D1 DE 60324810 D1 DE60324810 D1 DE 60324810D1 DE 60324810 T DE60324810 T DE 60324810T DE 60324810 T DE60324810 T DE 60324810T DE 60324810 D1 DE60324810 D1 DE 60324810D1
Authority
DE
Germany
Prior art keywords
helicase
sures
nuclear
dependent amplification
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60324810T
Other languages
English (en)
Inventor
Huimin Kong
Myriam Vincent
Yan Xu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New England Biolabs Inc
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=32033597&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60324810(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Application granted granted Critical
Publication of DE60324810D1 publication Critical patent/DE60324810D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
DE60324810T 2002-09-20 2003-09-19 HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN Expired - Lifetime DE60324810D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US41229802P 2002-09-20 2002-09-20
US44666203P 2003-02-11 2003-02-11
PCT/US2003/029020 WO2004027025A2 (en) 2002-09-20 2003-09-19 Helicase dependent amplification of nucleic acids

Publications (1)

Publication Number Publication Date
DE60324810D1 true DE60324810D1 (de) 2009-01-02

Family

ID=32033597

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60324810T Expired - Lifetime DE60324810D1 (de) 2002-09-20 2003-09-19 HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN

Country Status (8)

Country Link
US (3) US7282328B2 (de)
EP (1) EP1539979B1 (de)
JP (1) JP4632788B2 (de)
AT (1) ATE414792T1 (de)
AU (1) AU2003272438B2 (de)
CA (1) CA2498764C (de)
DE (1) DE60324810D1 (de)
WO (1) WO2004027025A2 (de)

Families Citing this family (264)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
ATE400663T1 (de) * 2002-02-21 2008-07-15 Asm Scient Inc Rekombinase-polymerase-amplifikation
US7662594B2 (en) 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
JP4903560B2 (ja) * 2003-04-25 2012-03-28 ベクトン・ディキンソン・アンド・カンパニー 核酸増幅法による単純ヘルペスウイルス1型および2型の検出
US20050069926A1 (en) * 2003-08-01 2005-03-31 Affymetrix, Inc. Helicase-amplified reverse transcription
EP1692486B1 (de) * 2003-12-12 2015-10-28 Saint Louis University Biosensoren zum nachweis von makromolekülen und anderen analyten
US7378242B2 (en) * 2004-03-18 2008-05-27 Atom Sciences, Inc. DNA sequence detection by limited primer extension
ES2718482T3 (es) * 2004-06-01 2019-07-02 Alere San Diego Inc Kit para amplificación de recombinasa polimerasa
US7700281B2 (en) * 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
US8158388B2 (en) * 2004-10-21 2012-04-17 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
KR20070073917A (ko) * 2004-10-21 2007-07-10 뉴 잉글랜드 바이오랩스, 인크 개선된 증폭을 위한 핵산 복구
US8883487B2 (en) 2004-12-23 2014-11-11 Abbott Point Of Care Inc. Molecular diagnostics system and methods
SI1833995T1 (sl) * 2005-01-05 2017-10-30 Biohelix Corporation Identifikacija tarč RNA z uporabo helikaz
EP1841879A4 (de) * 2005-01-25 2009-05-27 Population Genetics Technologi Isothermische dna-amplifikation
WO2006087574A2 (en) 2005-02-19 2006-08-24 Geneform Technologies Limited Isothermal nucleic acid amplification
US7888125B2 (en) 2005-05-09 2011-02-15 Theranos, Inc. Calibration of fluidic devices
US8956857B2 (en) 2005-06-06 2015-02-17 Mediomics, Llc Three-component biosensors for detecting macromolecules and other analytes
US7795009B2 (en) * 2005-06-15 2010-09-14 Saint Louis University Three-component biosensors for detecting macromolecules and other analytes
EP1907410B1 (de) 2005-06-10 2012-07-04 Saint Louis University Verfahren zur selektion von aptameren
US7811809B2 (en) * 2005-06-15 2010-10-12 Saint Louis University Molecular biosensors for use in competition assays
EP2385140B1 (de) 2005-07-25 2014-07-16 Alere San Diego, Inc. Verfahren zur Multiplexierung einer Rekombinase-Polymerase-Verstärkung
CA2621146A1 (en) * 2005-09-06 2007-03-15 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
US8703445B2 (en) 2005-12-29 2014-04-22 Abbott Point Of Care Inc. Molecular diagnostics amplification system and methods
WO2007087262A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Selective genome amplification
US11287421B2 (en) 2006-03-24 2022-03-29 Labrador Diagnostics Llc Systems and methods of sample processing and fluid control in a fluidic system
US8741230B2 (en) 2006-03-24 2014-06-03 Theranos, Inc. Systems and methods of sample processing and fluid control in a fluidic system
JP5654749B2 (ja) * 2006-04-11 2015-01-14 ニユー・イングランド・バイオレイブス・インコーポレイテツド 改良された増幅のための核酸の修復
WO2007120808A2 (en) * 2006-04-14 2007-10-25 Biohelix Corporation A method of asymmetric helicase-dependent amplification for probe-based detection of targets
JP2009535053A (ja) 2006-05-04 2009-10-01 エーエスエム サイエンティフィック, インコーポレイテッド レコンビナーゼポリメラーゼ増幅
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
PL2017356T3 (pl) 2006-06-06 2012-03-30 Gen Probe Inc Znakowane oligonukleotydy i ich zastosowanie w sposobach amplifikacji kwasu nukleinowego
JP4918409B2 (ja) 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US8980561B1 (en) 2006-08-22 2015-03-17 Los Alamos National Security, Llc. Nucleic acid detection system and method for detecting influenza
US20090047673A1 (en) * 2006-08-22 2009-02-19 Cary Robert B Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
WO2008058018A2 (en) 2006-11-02 2008-05-15 Mayo Foundation For Medical Education And Research Predicting cancer outcome
WO2008103900A2 (en) 2007-02-23 2008-08-28 New England Biolabs, Inc. Selection and enrichment of proteins using in vitro compartmentalization
DK2574681T3 (en) 2007-03-28 2016-07-04 Signal Diagnostics System and method for high-resolution analysis of nucleic acids to detect sequence variations
CA2691451C (en) 2007-06-21 2015-03-24 Sara H. Fan Instrument and receptacles for performing processes
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
SG10201606120XA (en) 2007-10-02 2016-09-29 Theranos Inc Modular Point-Of-Care Devices And Uses Thereof
US20090215050A1 (en) 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
EP2279403B1 (de) 2008-05-05 2016-03-16 Los Alamos National Security, LLC Höchst vereinfachte vorbereitung von nukleinsäureproben auf lateralflussbasis und passive strömungssteuerung
EP2806037B1 (de) 2008-05-13 2016-09-21 Gen-Probe Incorporated Inaktivierbare Target-Capture-Oligomere zur Verwendung bei der selektiven Hybridisierung und beim Fangen von Zielnukleinsäuresequenzen
EP2806054A1 (de) 2008-05-28 2014-11-26 Genomedx Biosciences Inc. Systeme und Verfahren zur expressionsbasierten Unterscheidung verschiedener klinischer Krankheitszustände bei Prostatakrebs
US10407731B2 (en) 2008-05-30 2019-09-10 Mayo Foundation For Medical Education And Research Biomarker panels for predicting prostate cancer outcomes
US9495515B1 (en) 2009-12-09 2016-11-15 Veracyte, Inc. Algorithms for disease diagnostics
US10236078B2 (en) 2008-11-17 2019-03-19 Veracyte, Inc. Methods for processing or analyzing a sample of thyroid tissue
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
EP2703816B1 (de) 2008-11-21 2016-10-05 Saint Louis University Biosensor zur erkennung mehrerer epitope auf einem ziel
JP2012516155A (ja) * 2009-01-27 2012-07-19 キアゲン ゲーザーズバーグ エンドポイント均一蛍光検出を用いた好熱性ヘリカーゼ依存性増幅技術
JP2010172248A (ja) * 2009-01-29 2010-08-12 National Institute Of Advanced Industrial Science & Technology 核酸の新規定量方法並びにそれに用いる新規試薬キット
CA2750900C (en) 2009-01-30 2017-03-28 Gen-Probe Incorporated Systems and methods for detecting a signal and applying thermal energy to a signal transmission element
WO2010091111A1 (en) 2009-02-03 2010-08-12 Biohelix Corporation Endonuclease-enhanced helicase-dependent amplification
US9074258B2 (en) 2009-03-04 2015-07-07 Genomedx Biosciences Inc. Compositions and methods for classifying thyroid nodule disease
WO2010123625A1 (en) 2009-04-24 2010-10-28 University Of Southern California Cd133 polymorphisms predict clinical outcome in patients with cancer
US8669057B2 (en) 2009-05-07 2014-03-11 Veracyte, Inc. Methods and compositions for diagnosis of thyroid conditions
WO2010135310A1 (en) 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
EP3663750B1 (de) 2009-05-29 2021-11-03 Life Technologies Corporation Nukleinsäuregerüstpolymerpartikel und verfahren zur herstellung und verwendung
EP3360974A1 (de) 2009-06-05 2018-08-15 Alere San Diego, Inc. Reagentien zur rekombinase polymerase amplifikation
US8628924B2 (en) 2009-07-21 2014-01-14 Gen-Probe Incorporated Methods and compositions for quantitative amplification and detection over a wide dynamic range
CA2770888C (en) 2009-08-31 2020-04-14 Gen-Probe Incorporated Dengue virus assay
US8951733B2 (en) 2009-10-16 2015-02-10 Monsanto Technology Llc Methods of polynucleotide detection
US9121054B2 (en) * 2009-12-08 2015-09-01 Biohelix Corporation Detection of nucleic acid amplification products in the presence of an internal control sequence on an immunochromatographic strip
US10446272B2 (en) 2009-12-09 2019-10-15 Veracyte, Inc. Methods and compositions for classification of samples
WO2011084757A1 (en) 2009-12-21 2011-07-14 University Of Southern California Germline polymorphisms in the sparc gene associated with clinical outcome in gastric cancer
CA2786473A1 (en) * 2010-01-08 2011-07-14 Qiagen Gaithersburg, Inc. Materials and methods for isothermal nucleic acid amplification
WO2011085334A1 (en) 2010-01-11 2011-07-14 University Of Southern California Cd44 polymorphisms predict clinical outcome in patients with gastric cancer
WO2011100561A1 (en) 2010-02-12 2011-08-18 Saint Louis University Molecular biosensors capable of signal amplification
WO2011106634A2 (en) 2010-02-26 2011-09-01 Life Technologies Corporation Modified proteins and methods of making and using same
DK2556171T3 (en) 2010-04-05 2015-12-14 Prognosys Biosciences Inc Spatially CODED BIOLOGICAL ASSAYS
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
WO2011150227A1 (en) 2010-05-26 2011-12-01 Qiagen Gaithersburg, Inc. Quantitative helicase assay
WO2012003289A1 (en) 2010-06-30 2012-01-05 Gen-Probe Incorporated Method and apparatus for identifying analyte-containing samples using single-read determination of analyte and process control signals
US9121053B2 (en) * 2010-07-08 2015-09-01 University Of Central Florida Research Foundation, Inc. Method of detecting single nucleotide polymorphisms
EP2420579A1 (de) 2010-08-17 2012-02-22 QIAGEN GmbH Helikasenabhängige isothermale Amplifikation mit Nicking-Enzymen
CN103270173B (zh) 2010-10-12 2017-11-21 孟山都技术公司 对应于转基因事件mon87712的大豆植物和种子及其检测方法
CN106248582B (zh) 2011-01-21 2020-10-20 拉布拉多诊断有限责任公司 样品使用最大化的系统和方法
EP3978620A1 (de) 2011-04-07 2022-04-06 Abbott Diagnostics Scarborough, Inc. Überwachung von rekombinase-polymerase-verstärkungsmischungen
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
DK2699700T3 (en) 2011-04-20 2016-08-01 Mesa Biotech Inc Integrated device for nukleinsyreregistrering and identification
JP6223332B2 (ja) 2011-06-30 2017-11-01 モンサント テクノロジー エルエルシー 形質転換事象kk179−2に対応するアルファルファ植物および種子、ならびにその検出方法
US20130260422A1 (en) 2011-09-01 2013-10-03 New England Biolabs, Inc. Compositions and Methods Relating to Variant DNA Polymerases and Synthetic DNA Polymerases
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
US8380541B1 (en) 2011-09-25 2013-02-19 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US8475739B2 (en) 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US8435738B2 (en) 2011-09-25 2013-05-07 Theranos, Inc. Systems and methods for multi-analysis
US9268915B2 (en) 2011-09-25 2016-02-23 Theranos, Inc. Systems and methods for diagnosis or treatment
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US8840838B2 (en) 2011-09-25 2014-09-23 Theranos, Inc. Centrifuge configurations
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
EP2758547B1 (de) 2011-09-21 2015-09-16 Gen-Probe Incorporated Verfahren zur amplifikation von nukleinsäuren unter verwendung von tag-vermittelter verschiebung
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
BR112014007073A2 (pt) 2011-09-25 2017-04-11 Theranos Inc sistemas e métodos para multi-análise
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
US9250229B2 (en) 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
KR20140090633A (ko) 2011-10-21 2014-07-17 옥스포드 나노포어 테크놀로지즈 리미티드 포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법
EP2791359B1 (de) 2011-12-13 2020-01-15 Decipher Biosciences, Inc. Krebsdiagnostik mit nichtcodierenden transkripten
CN104126018B (zh) 2011-12-29 2021-09-14 牛津纳米孔技术公司 酶方法
CA2881200A1 (en) 2012-02-14 2013-08-22 Great Basin Scientific Methods of isothermal amplification using blocked primers
WO2013121201A1 (en) 2012-02-15 2013-08-22 Oxford Nanopore Technologies Limited Aptamer method
CN114134207A (zh) 2012-04-09 2022-03-04 一龙公司 用于量化样品中核酸序列的组合物和方法
EP2859111B1 (de) 2012-06-08 2020-07-08 Ionian Technologies, LLC Nukleinsäureamplifizierungen
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
WO2014013262A1 (en) * 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Enzyme construct
EP2875128B8 (de) 2012-07-19 2020-06-24 Oxford Nanopore Technologies Limited Modifizierte helikasen
AU2013202793B2 (en) 2012-07-31 2014-09-18 Gen-Probe Incorporated System, method and apparatus for automated incubation
EP3435084B1 (de) 2012-08-16 2023-02-22 Decipher Biosciences, Inc. Prostatakrebsprognose unter verwendung von biomarkern
US8993298B1 (en) 2012-08-31 2015-03-31 New England Biolabs, Inc. DNA polymerases
MX2015002919A (es) 2012-09-11 2015-08-14 Theranos Inc Sistemas y metodos de manejo de informacion que utilizan una firma biologica.
CN102912038B (zh) * 2012-10-25 2014-05-28 中华人民共和国北京出入境检验检疫局 一种检测禽流感病毒的rt-hda试剂盒及引物
CN102876813B (zh) * 2012-10-25 2013-11-13 中华人民共和国北京出入境检验检疫局 一种检测禽流感病毒的实时荧光rt-hda试剂盒及引物
US9074248B1 (en) 2012-12-18 2015-07-07 Qiagen Gmbh Primers for helicase dependent amplification and their methods of use
WO2014095931A1 (en) * 2012-12-18 2014-06-26 Qiagen Gmbh Nucleic acid amplification method
MX354033B (es) 2013-02-18 2018-02-09 Theranos Ip Co Llc Sistemas y métodos para recolectar y transmitir resultados de ensayos.
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
US10975423B2 (en) 2013-03-11 2021-04-13 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
CN104707674B (zh) 2013-03-11 2018-06-15 克忧公司 用于检测和量化分析物的系统和方法
US9623409B2 (en) 2013-03-11 2017-04-18 Cue Inc. Cartridges, kits, and methods for enhanced mixing for detection and quantification of analytes
EP2971106B1 (de) 2013-03-11 2020-02-26 ELITechGroup, Inc. Verfahren für echte isothermale strangverschiebungsamplifikation
US10590474B2 (en) 2013-03-11 2020-03-17 Elitechgroup B.V. Methods for true isothermal strand displacement amplification
US10545161B2 (en) 2013-03-11 2020-01-28 Cue Health Inc. Systems and methods for detection and quantification of analytes
CN111662960B (zh) 2013-06-25 2024-04-12 普罗格诺西斯生物科学公司 采用微流控装置的空间编码生物分析
US9920358B2 (en) 2013-06-27 2018-03-20 New England Biolabs, Inc. Helicase suppression of non-template amplification
US10392670B2 (en) 2013-07-25 2019-08-27 Dch Molecular Diagnostics, Inc. Methods and compositions for detecting bacterial contamination
US9315842B2 (en) * 2013-08-19 2016-04-19 Steven A Benner Helicase dependent amplification of DNA molecules using nucleotide analogs
EP3054764B1 (de) 2013-10-09 2018-12-05 Monsanto Technology LLC Transgenes ereignis mon87403 in mais und nachweisverfahren dafür
AU2014331828B2 (en) 2013-10-09 2020-05-14 Fluoresentric, Inc. Multiplex probes
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
EP3058088A2 (de) 2013-10-18 2016-08-24 Oxford Nanopore Technologies Limited Modifizierte enzyme
MX367192B (es) 2013-10-30 2019-08-08 Green Life Biotech Llc Sistemas de cuantificacion de patogenesis y metodos de tratamiento para el enverdecimiento de los citricos.
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
CN109706222A (zh) 2013-12-11 2019-05-03 安可济控股有限公司 用于检测罕见序列变体的组合物和方法
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
BR112016024622A2 (pt) 2014-04-22 2017-10-10 Envirologix Inc composições e métodos para melhorar e/ou prever a amplificação de dna
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
USD745423S1 (en) 2014-05-12 2015-12-15 Cue Inc. Automated analyzer test cartridge and sample collection device for analyte detection
EP3169793B1 (de) 2014-07-16 2022-07-06 Tangen Biosciences Inc. Isothermische verfahren zur amplifizierung von nukleinsäureproben
WO2016013620A1 (ja) * 2014-07-24 2016-01-28 学校法人関西学院 好熱性アーキア由来DEAD-box型RNAへリカーゼ及びその改変体を用いるDNA増幅方法、並びにその方法のために使用する好熱性アーキア由来DEAD-box型RNAへリカーゼ及びその改変体
WO2016040830A1 (en) 2014-09-12 2016-03-17 Mediomics, Llc Molecular biosensors with a modular design
EP3207155B1 (de) * 2014-10-17 2019-06-05 Oxford Nanopore Technologies Limited Verfahren zur charakterisierung von nanoporen-rna
US11559801B2 (en) 2014-11-03 2023-01-24 Tangen Biosciences, Inc. Apparatus and method for cell, spore, or virus capture and disruption
US20170335396A1 (en) 2014-11-05 2017-11-23 Veracyte, Inc. Systems and methods of diagnosing idiopathic pulmonary fibrosis on transbronchial biopsies using machine learning and high dimensional transcriptional data
US10619204B2 (en) 2014-11-11 2020-04-14 Illumina Cambridge Limited Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
EP3218467A4 (de) 2014-11-13 2018-04-11 The Board of Trustees of the University of Illinois Biologisch manipulierte hyperfunktionale »super -helikasen
PT3234187T (pt) 2014-12-15 2021-05-26 Illumina Inc Método para colocação de moleculas de alvo unico num substrato
RU2755495C2 (ru) 2015-01-30 2021-09-16 Инвайролоджикс Инк. Молекула субстрата
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
DK3901281T3 (da) 2015-04-10 2023-01-23 Spatial Transcriptomics Ab Rumligt adskilt, multipleks nukleinsyreanalyse af biologiske prøver
WO2016168755A1 (en) 2015-04-17 2016-10-20 Distributed Bio, Inc. Method for mass humanization of non-human antibodies
AU2016252998B2 (en) 2015-04-24 2021-11-04 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
EP3303611B1 (de) 2015-05-29 2019-10-02 Roche Diagnostics GmbH Verfahren zur inaktivierung von mikroben durch citraconsäureanhydrid
EP3303614B1 (de) 2015-05-29 2020-03-11 Illumina Cambridge Limited Verbesserte verwendung von oberflächenprimern bei clustern
CN108136391B (zh) 2015-07-17 2021-01-26 克忧健康公司 用于增强检测和分析物定量的系统及方法
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
US10781494B2 (en) 2015-09-15 2020-09-22 Neogen Corporation Methods of detecting listeria from an environmental sample
EP3359693A4 (de) 2015-10-09 2019-03-06 Accuragen Holdings Limited Verfahren und zusammensetzungen zur anreicherung von amplifikationsprodukten
EP3383994A4 (de) 2015-12-03 2019-08-28 Accuragen Holdings Limited Verfahren und zusammensetzungen zum formen von ligationsprodukten
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
AU2017246899B2 (en) 2016-04-07 2020-04-09 Illumina, Inc. Methods and systems for construction of normalized nucleic acid libraries
EP3458586B1 (de) 2016-05-16 2022-12-28 Accuragen Holdings Limited Verfahren zur verbesserten sequenzierung durch strangidentifizierung
EP3464628B1 (de) 2016-06-06 2024-01-31 RedVault Biosciences, LP Targetreporterkonstrukte und verwendungen davon
JP7155021B2 (ja) 2016-07-22 2022-10-18 オレゴン ヘルス アンド サイエンス ユニヴァーシティ 単細胞全ゲノムライブラリおよびそれを作成する組み合わせインデックス付加方法
JP6853523B2 (ja) * 2016-07-27 2021-03-31 国立大学法人京都大学 ヘリカーゼを用いたpcr
WO2018035170A1 (en) 2016-08-15 2018-02-22 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
CN110506127B (zh) 2016-08-24 2024-01-12 维拉科特Sd公司 基因组标签预测前列腺癌患者对术后放射疗法应答性的用途
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
CN107058287B (zh) * 2017-01-16 2020-06-16 浙江大学 一种恒温扩增体系中生成单链产物的方法
AU2018210695A1 (en) 2017-01-20 2019-08-08 The University Of British Columbia Molecular subtyping, prognosis, and treatment of bladder cancer
US11237161B2 (en) 2017-01-25 2022-02-01 Cue Health Inc. Systems and methods for enhanced detection and quantification of analytes
WO2018165600A1 (en) 2017-03-09 2018-09-13 Genomedx Biosciences, Inc. Subtyping prostate cancer to predict response to hormone therapy
JP6448695B2 (ja) 2017-03-21 2019-01-09 株式会社東芝 Rna増幅方法、rna検出方法及びアッセイキット
WO2018175883A1 (en) 2017-03-24 2018-09-27 Gen-Probe Incorporated Compositions and methods for detecting or quantifying parainfluenza virus
DK3872187T3 (da) 2017-04-23 2022-12-05 Illumina Cambridge Ltd Sammensætninger og fremgangsmåder til forbedring af prøveidentificering i indekserede nukleinsyrebiblioteker
ES2937929T3 (es) 2017-04-23 2023-04-03 Illumina Inc Composiciones y métodos para mejorar la identificación de muestras en bibliotecas de ácido nucleico indexadas
AU2018259202B2 (en) 2017-04-23 2022-03-24 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
WO2018197950A1 (en) 2017-04-23 2018-11-01 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
US11078542B2 (en) 2017-05-12 2021-08-03 Decipher Biosciences, Inc. Genetic signatures to predict prostate cancer metastasis and identify tumor aggressiveness
DK3635136T3 (da) 2017-06-07 2022-01-10 Univ Oregon Health & Science Enkeltcelle-helgenombiblioteker til methyleringssekvensering
US11217329B1 (en) 2017-06-23 2022-01-04 Veracyte, Inc. Methods and systems for determining biological sample integrity
CN111032209A (zh) 2017-07-10 2020-04-17 简·探针公司 分析系统和方法
WO2019027767A1 (en) 2017-07-31 2019-02-07 Illumina Inc. SEQUENCING SYSTEM COMPRISING AGGREGATION OF MULTIPLEXED BIOLOGICAL SAMPLES
US20190100811A1 (en) 2017-10-02 2019-04-04 Quidel Corporation Phage-based detection method for antimicrobial susceptibility testing and identification of bacterial species
WO2019118550A1 (en) 2017-12-13 2019-06-20 Gen-Probe Incorporated Methods for biological sample processing
US20200318171A1 (en) 2017-12-15 2020-10-08 Gen-Probe Incorporated Compositions and Methods for Detecting Toxigenic Clostridium Difficile
US20210071242A1 (en) 2018-01-29 2021-03-11 Gen-Probe Incorporated Analytical systems and methods
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
JP6974504B2 (ja) 2018-04-02 2021-12-01 イルミナ インコーポレイテッド 配列に基づく遺伝子試験のための対照を作製するための組成物及び方法
CN108588050B (zh) * 2018-05-14 2021-06-25 北京艾克伦医疗科技有限公司 Dna聚合酶以及核酸检测方法和试剂盒
EP4269618A3 (de) 2018-06-04 2024-01-10 Illumina, Inc. Verfahren zur herstellung von einzelzelltranskriptombibliotheken mit hohem durchsatz
GB2597567B (en) 2018-06-13 2022-11-23 Gen Probe Inc Compositions and methods for detecting group B streptococcus nucleic acid
EP3814527B1 (de) * 2018-06-26 2023-02-22 Massachusetts Institute of Technology Verfahren, systeme und diagnostika auf der basis von crispr-effektorsystemen
US20210317515A1 (en) 2018-07-10 2021-10-14 Gen-Probe Incorporated Methods and systems for detecting and quantifying nucleic acids
EP3830302B1 (de) 2018-08-01 2022-10-05 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von nukleinsäuren des epstein-barr-virus
EP3841223A1 (de) 2018-08-08 2021-06-30 Gen-Probe Incorporated Zusammensetzungen, verfahren und kits zum nachweis von mycoplasma genitalium
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020069085A2 (en) 2018-09-27 2020-04-02 Gen-Probe Incorporated Compositions and methods for detecting bordetella pertussis and bordetella parapertussis nucleic acid
WO2020123320A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Imaging system hardware
PL3899037T3 (pl) 2018-12-19 2024-04-08 Illumina, Inc. Sposoby poprawy priorytetu klonalności klastrów polinukleotydowych
WO2020142347A2 (en) 2018-12-31 2020-07-09 Gen-Probe Incorporated Systems and methods for filling multi-well cartridges with solid reagents
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3931354A1 (de) 2019-02-28 2022-01-05 10X Genomics, Inc. Profilierung von biologischen analyten mit räumlich barcodierten oligonukleotid-arrays
BR112021006234A2 (pt) 2019-03-01 2021-09-28 Illumina, Inc. Bibliotecas de células únicas e núcleos únicos de alto rendimento e métodos de preparo e uso
EP3937780A4 (de) 2019-03-14 2022-12-07 InSilixa, Inc. Verfahren und systeme zur zeitgesteuerten fluoreszenzbasierten detektion
EP3938538A1 (de) 2019-03-15 2022-01-19 10X Genomics, Inc. Verfahren zur verwendung von räumlichen anordnungen für einzelzellsequenzierung
US20220098647A1 (en) 2019-03-22 2022-03-31 Gen-Probe Incorporated Compositions and Methods for Detecting Group A Streptococcus
WO2020198071A1 (en) 2019-03-22 2020-10-01 10X Genomics, Inc. Three-dimensional spatial analysis
CA3176696A1 (en) 2019-05-03 2020-11-12 Gen-Probe Incorporated Receptacle transport system for an analytical system
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
AU2020335996A1 (en) 2019-08-23 2022-03-31 Gen-Probe Incorporated Compositions, methods and kits for detecting Treponema pallidum
CA3153514A1 (en) 2019-09-05 2021-03-11 Gen-Probe Incorporated Detection of chlamydia trachomatis nucleic acid variants
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
CN114600215A (zh) 2019-10-30 2022-06-07 三菱电机株式会社 螺线管装置以及起动器
EP4025711A2 (de) 2019-11-08 2022-07-13 10X Genomics, Inc. Erhöhung der spezifität einer analytbindung
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
US20230002812A1 (en) 2019-11-13 2023-01-05 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2021102003A1 (en) 2019-11-18 2021-05-27 10X Genomics, Inc. Systems and methods for tissue classification
EP4062373A1 (de) 2019-11-21 2022-09-28 10X Genomics, Inc. Räumliche analyse von analyten
CN115023734B (zh) 2019-11-22 2023-10-20 10X基因组学有限公司 使用基准对齐对分析物进行空间分析的系统和方法
CN114008199A (zh) 2019-12-19 2022-02-01 伊路敏纳公司 高通量单细胞文库及其制备和使用方法
ES2946357T3 (es) 2019-12-23 2023-07-17 10X Genomics Inc Métodos para el análisis espacial usando ligación con molde de ARN
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
IL296604A (en) 2020-03-30 2022-11-01 Illumina Inc Methods and preparations for preparing nucleic acid libraries
EP4242325A3 (de) 2020-04-22 2023-10-04 10X Genomics, Inc. Verfahren zur räumlichen analyse unter verwendung von gezieltem rna-abbau
JP2023524117A (ja) 2020-04-30 2023-06-08 スタブ ヴィーダ - インヴェスティガサォン エ セルヴィソス エム シエンシアス バイオロジカス エルディーエー コロナウイルスの疑いのある試料中の核酸配列を検出するための方法及びポータブルデバイス
CA3177286A1 (en) 2020-05-12 2021-11-18 Illumina Inc. Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
EP4165207A1 (de) 2020-06-10 2023-04-19 10X Genomics, Inc. Verfahren zur bestimmung einer position eines analyten in einer biologischen probe
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
CA3177299A1 (en) 2020-06-30 2022-01-06 Illumina Inc. Catalytically controlled sequencing by synthesis to produce scarless dna
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
AU2021308095A1 (en) 2020-07-17 2023-03-09 Gen-Probe Incorporated Detection of macrolide-resistant mycoplasma genitalium
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
CN116323440A (zh) 2020-10-21 2023-06-23 简·探针公司 流体容器管理系统
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4121555A1 (de) 2020-12-21 2023-01-25 10X Genomics, Inc. Verfahren, zusammensetzungen und systeme zur erfassung von sonden und/oder barcodes
WO2022165188A1 (en) 2021-01-29 2022-08-04 Illumina, Inc. Methods, compositions and kits to improve seeding efficiency of flow cells with polynucleotides
WO2022197634A1 (en) 2021-03-15 2022-09-22 Gen-Probe Incorporated Compositions and methods for biological sample processing
EP4314327A1 (de) 2021-03-22 2024-02-07 Illumina Cambridge Limited Verfahren zur verbesserung der klonalität von nukleinsäureclustern
US20220396778A1 (en) 2021-06-12 2022-12-15 Shi-Lung Lin Novel RNA Composition and Production Method for Use in iPS Cell Generation
AU2022319876A1 (en) 2021-07-27 2024-01-18 Gen-Probe Incorporated Compositions and methods for detecting gastrointestinal pathogens
WO2023014222A1 (en) 2021-08-03 2023-02-09 Wageningen Universiteit Argonaute-based nucleic acid detection system
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
US20230279462A1 (en) * 2022-03-01 2023-09-07 Spectacular Labs, Inc. Methods for detecting microorganisms
WO2023196572A1 (en) 2022-04-07 2023-10-12 Illumina Singapore Pte. Ltd. Altered cytidine deaminases and methods of use
EP4282980A1 (de) 2022-05-23 2023-11-29 Mobidiag Oy Methoden zum verstärken einer nukleinsäure
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5176995A (en) * 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5541099A (en) * 1989-08-10 1996-07-30 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase reduced in 3'-to-5' exonuclease activity
US5494810A (en) * 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
EP0894860B1 (de) * 1990-09-28 2012-12-12 F. Hoffmann-La Roche AG Thermostabile DNA Polymerasen und deren Deletionsmutanten
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
JPH05146299A (ja) 1991-11-27 1993-06-15 Toyobo Co Ltd 核酸配列の増幅方法およびそのための試薬キツト
US6555349B1 (en) * 1993-01-22 2003-04-29 Cornell Research Foundation, Inc. Methods for amplifying and sequencing nucleic acid molecules using a three component polymerase
US5714320A (en) * 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US5470723A (en) * 1993-05-05 1995-11-28 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
JPH0716094A (ja) 1993-06-15 1995-01-20 Toyobo Co Ltd Rnaポリメラーゼのプロモーター配列をもつ1本鎖オリゴヌクレオチドを製造する方法
AU687535B2 (en) * 1994-03-16 1998-02-26 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
US6306588B1 (en) * 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
AU2228599A (en) * 1998-01-26 1999-08-09 Saigene Corporation An exponential nucleic acid amplification method using a single primer of opposing polarity
US6218122B1 (en) 1998-06-19 2001-04-17 Rosetta Inpharmatics, Inc. Methods of monitoring disease states and therapies using gene expression profiles
US6235502B1 (en) * 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
WO2000041524A2 (en) * 1999-01-11 2000-07-20 President And Fellows Of Harvard College Isothermal amplification of dna
KR20020079364A (ko) 1999-06-28 2002-10-19 소스 프리시전 메디슨, 인코포레이티드 보정된 유전자 발현 프로파일을 이용한 생물학적 상태또는 물질을 특성화하기 위한 시스템 및 방법
AU6385800A (en) * 1999-07-30 2001-02-19 Stratagene Archaeal replication accessory factors and methods of use
WO2002002740A2 (en) 2000-07-05 2002-01-10 Rosetta Inpharmatics, Inc. Methods and compositions for determining gene function
EP1386006B1 (de) * 2001-04-20 2008-05-28 The Penn State Research Foundation Verfahren zur manipulation von nukleinsäuren
US6977148B2 (en) * 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
ATE400663T1 (de) * 2002-02-21 2008-07-15 Asm Scient Inc Rekombinase-polymerase-amplifikation

Also Published As

Publication number Publication date
JP4632788B2 (ja) 2011-02-16
AU2003272438B2 (en) 2009-04-02
US20040058378A1 (en) 2004-03-25
EP1539979A2 (de) 2005-06-15
US7829284B2 (en) 2010-11-09
CA2498764C (en) 2015-11-10
JP2006500028A (ja) 2006-01-05
WO2004027025A2 (en) 2004-04-01
US7282328B2 (en) 2007-10-16
US20070207495A1 (en) 2007-09-06
US20070254304A1 (en) 2007-11-01
AU2003272438A1 (en) 2004-04-08
EP1539979A4 (de) 2006-01-11
EP1539979B1 (de) 2008-11-19
WO2004027025A3 (en) 2004-07-08
CA2498764A1 (en) 2004-04-01
ATE414792T1 (de) 2008-12-15

Similar Documents

Publication Publication Date Title
DE60324810D1 (de) HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN
HUP0302803A2 (hu) Nukleinsavak izotermikus amplifikációja szilárd hordozón
ATE329059T1 (de) Eine vollständig intern kontrollierte amplifikationsreaktion ermöglichende zusammensetzungen und verfahren
DE602004025801D1 (de) Dna-polymerasefusionen und deren verwendungen
ATE483194T1 (de) Verfahren und system zum auswählen und manipulieren von mehreren objekten
WO2004048931A3 (en) Compositions, methods and kits for polynucleotide amplification reactions and microfluidic devices
BRPI0413949A (pt) aparelho e sistema para a manipulação de entidades celulares
ATE437945T1 (de) Verfahren zur amplifikation und sequenzierung von nukleinsäuren
DK1576132T3 (da) Nukleinsyrer benævnt 273p4b7 og anvendelse heraf til detektering af cancer
DK1252328T3 (da) Fremgangsmåde og midler til isolering af mikroorganismer
WO2002101022A3 (en) Method for rapid amplification of dna
TW200616578A (en) Fluid handling devices
DK0702090T3 (da) Coamplifikation af målnucleinsyrer ved anvendelse af volumenudelukkelsesmiddel i reaktionssammensætning, testkit og testanordning, som er anvendelige dertil
MX2007012486A (es) Ensayos de investigacion sobre estilbazio.
WO2006030455A8 (en) Dna polymerases having strand displacement activity
EP1065213A3 (de) HCV Polymerase die für eine Krystalstruktur Analyse geeignet ist, und Verfahren zur Benutzung diesem Enzym
ATE439441T1 (de) Veränderte, fluoreszierende proteine
DK1465927T3 (da) BAG3-antistoffer til anvendelse til forskning, diagnostik og behandling af sygdomme, der omfatter celledød
ATE403663T1 (de) Nukleinsäureamplifikation mit duplexzwischenprodukten
ATE526418T1 (de) Verbesserte zusammensetzungen zur in-vitro- amplifikation von nukleinsäuren
DE60236996D1 (de) Kulturmedium zum nachweis von bakterien der gattung listeria
AU2002367750A8 (en) Isothermal amplification in nucleic acid analysis
WO2008146649A1 (ja) 酵素含有ゲル及び核酸の増幅キット
DE602004009036D1 (de) Verbessertes verfahren zum nachweis von proteolytischen enzymen
ATE340272T1 (de) In gegenwart von collagen und disacchariden lyophilisierte nukleinsäuren umfassende zusammensetzung

Legal Events

Date Code Title Description
8363 Opposition against the patent