DE60325743D1 - Nanopartikel-polyanion-konjugate sowie verfahren zur verwendung davon beim nachweis von analyten - Google Patents

Nanopartikel-polyanion-konjugate sowie verfahren zur verwendung davon beim nachweis von analyten

Info

Publication number
DE60325743D1
DE60325743D1 DE60325743T DE60325743T DE60325743D1 DE 60325743 D1 DE60325743 D1 DE 60325743D1 DE 60325743 T DE60325743 T DE 60325743T DE 60325743 T DE60325743 T DE 60325743T DE 60325743 D1 DE60325743 D1 DE 60325743D1
Authority
DE
Germany
Prior art keywords
sub
line
formulae
polyanion
polyanionic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60325743T
Other languages
English (en)
Inventor
James J Storhoff
Robert L Letsinger
Susan R Hetzel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanosphere LLC
Original Assignee
Nanosphere LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanosphere LLC filed Critical Nanosphere LLC
Application granted granted Critical
Publication of DE60325743D1 publication Critical patent/DE60325743D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/70—Nanostructure
    • Y10S977/701—Integrated with dissimilar structures on a common substrate
    • Y10S977/702—Integrated with dissimilar structures on a common substrate having biological material component
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2982—Particulate matter [e.g., sphere, flake, etc.]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Nanotechnology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Materials Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Other Resins Obtained By Reactions Not Involving Carbon-To-Carbon Unsaturated Bonds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
DE60325743T 2002-07-02 2003-07-02 Nanopartikel-polyanion-konjugate sowie verfahren zur verwendung davon beim nachweis von analyten Expired - Lifetime DE60325743D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39325502P 2002-07-02 2002-07-02
PCT/US2003/021021 WO2004004647A2 (en) 2002-07-02 2003-07-02 Nanoparticle polyanion conjugates and methods of use thereof in detecting analytes

Publications (1)

Publication Number Publication Date
DE60325743D1 true DE60325743D1 (de) 2009-02-26

Family

ID=30115557

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60325743T Expired - Lifetime DE60325743D1 (de) 2002-07-02 2003-07-02 Nanopartikel-polyanion-konjugate sowie verfahren zur verwendung davon beim nachweis von analyten

Country Status (8)

Country Link
US (2) US7253277B2 (de)
EP (1) EP1540006B1 (de)
JP (1) JP2005532456A (de)
AT (1) ATE420357T1 (de)
AU (1) AU2003247788B2 (de)
CA (1) CA2490413C (de)
DE (1) DE60325743D1 (de)
WO (1) WO2004004647A2 (de)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6582921B2 (en) 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US6984491B2 (en) * 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7098320B1 (en) 1996-07-29 2006-08-29 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6750016B2 (en) * 1996-07-29 2004-06-15 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6506564B1 (en) * 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7169556B2 (en) * 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6974669B2 (en) * 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US20050037397A1 (en) * 2001-03-28 2005-02-17 Nanosphere, Inc. Bio-barcode based detection of target analytes
AU774593C (en) * 2000-01-13 2005-06-23 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
AU7687001A (en) * 2000-07-11 2002-01-21 Nanospherre Inc Method of detection by enhancement of silver staining
US20060040286A1 (en) * 2001-03-28 2006-02-23 Nanosphere, Inc. Bio-barcode based detection of target analytes
US20030113740A1 (en) * 2001-04-26 2003-06-19 Mirkin Chad A. Oligonucleotide-modified ROMP polymers and co-polymers
US7147687B2 (en) * 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7238472B2 (en) 2001-05-25 2007-07-03 Nanosphere, Inc. Non-alloying core shell nanoparticles
AU2002365115A1 (en) * 2001-07-20 2003-09-02 North Carolina State University Light addressable electrochemical detection of duplex structures
US20030211488A1 (en) 2002-05-07 2003-11-13 Northwestern University Nanoparticle probs with Raman spectrocopic fingerprints for analyte detection
JP2005532456A (ja) * 2002-07-02 2005-10-27 ナノスフェアー　インコーポレイテッド ナノ粒子ポリアニオン複合物ならびに分析物の検出における同複合物の用途
US20040180369A1 (en) * 2003-01-16 2004-09-16 North Carolina State University Photothermal detection of nucleic acid hybridization
EP1597399A2 (de) * 2003-02-27 2005-11-23 Nanosphere, Inc. Markierungsfreie erstellung von genexpressionsprofilen mittels universeller nanopartikelsonden im mikroarray-testformat
US20050250094A1 (en) * 2003-05-30 2005-11-10 Nanosphere, Inc. Method for detecting analytes based on evanescent illumination and scatter-based detection of nanoparticle probe complexes
US20060240573A1 (en) * 2003-07-29 2006-10-26 Lamdagen, Llc Optical system including nanostructures for biological or chemical sensing
US20100000881A1 (en) * 2003-10-30 2010-01-07 North Carolina State University Electrochemical detection of nucleic acid hybridization
US20050191651A1 (en) * 2003-10-30 2005-09-01 North Carolina State University Temperature-jump enhanced electrochemical detection of nucleic acid hybridization
CA2572178A1 (en) * 2004-07-26 2006-03-16 Nanosphere, Inc. Method for distinguishing methicillin resistant s. aureus from methicillin sensitive s. aureus in a mixed culture
US7738096B2 (en) * 2004-10-21 2010-06-15 University Of Georgia Research Foundation, Inc. Surface enhanced Raman spectroscopy (SERS) systems, substrates, fabrication thereof, and methods of use thereof
US7583379B2 (en) * 2005-07-28 2009-09-01 University Of Georgia Research Foundation Surface enhanced raman spectroscopy (SERS) systems and methods of use thereof
US20060134806A1 (en) * 2004-12-20 2006-06-22 General Electric Company Method of separating unattached Raman-active tag from bioassay or other reaction mixture
WO2007002567A2 (en) * 2005-06-23 2007-01-04 Nanosphere, Inc. Selective isolation and concentration of nucleic acids from complex samples
ATE489482T1 (de) * 2005-08-19 2010-12-15 Nanosphere Inc Verfahren zur herstellung von hybridsubstraten mit dna und antikörpern sowie verwendungen davon
US20070072309A1 (en) * 2005-09-29 2007-03-29 General Electric Company Analytical compositions including nanometer-sized transducers, methods to make thereof, and devices therefrom
JP5400297B2 (ja) * 2005-12-27 2014-01-29 古河電気工業株式会社 蛍光ナノシリカ粒子、ナノ蛍光材料、それを用いたバイオチップ及びそのアッセイ法
WO2008141289A1 (en) * 2007-05-10 2008-11-20 Northwestern University Silver nanoparticle binding agent conjugates based on moieties with triple cyclic disulfide anchoring groups
EP3733871A1 (de) * 2008-05-27 2020-11-04 Dako Denmark A/S Zusammensetzungen und verfahren zur erkennung von chromosomalen abweichungen mit neuen hybridisierungspuffern
SG173636A1 (en) * 2009-02-13 2011-09-29 Agency Science Tech & Res Label-free method for detecting presence or absence of nucleic acids
WO2010097655A1 (en) 2009-02-26 2010-09-02 Dako Denmark A/S Compositions and methods for rna hybridization applications
CA2760774A1 (en) * 2009-05-05 2010-11-11 Altermune Technologies, Llc Chemically programmable immunity
FR2952300B1 (fr) * 2009-11-09 2012-05-11 Oreal Nouveaux colorants fluorescents a motif heterocyclique disulfure, composition de teinture les comprenant et procede de coloration des fibres keratiniques humaines a partir de ces colorants
US10662465B2 (en) 2011-09-30 2020-05-26 Agilent Technologies, Inc. Hybridization compositions and methods using formamide
EP2768974B1 (de) 2011-10-21 2017-07-19 Dako Denmark A/S Hybridisierungszusammensetzungen und -verfahren
US10948491B2 (en) 2015-08-11 2021-03-16 University Of Central Florida Research Foundation, Inc. Passive insect surveillance sensor device
ES2944589T3 (es) 2016-08-25 2023-06-22 Hoffmann La Roche Nanopartículas de silicio multifuncionalizadas y usos de las mismas en procedimientos de detección basados en electroquimioluminiscencia
WO2018076025A1 (en) * 2016-10-21 2018-04-26 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Molecular nanotags
US20200124594A1 (en) * 2017-01-12 2020-04-23 Agency For Science, Technology And Research Method of Detecting the Presence of Different Target Analytes and Related Kits Thereof
CN107643277B (zh) * 2017-08-16 2020-01-07 广西师范大学 适配体调控量子点催化表面增强拉曼光谱测定Pb2+的方法

Family Cites Families (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4193983A (en) * 1978-05-16 1980-03-18 Syva Company Labeled liposome particle compositions and immunoassays therewith
NL7807532A (nl) * 1978-07-13 1980-01-15 Akzo Nv Metaal-immunotest.
US4318707A (en) * 1978-11-24 1982-03-09 Syva Company Macromolecular fluorescent quencher particle in specific receptor assays
US4256834A (en) * 1979-04-09 1981-03-17 Syva Company Fluorescent scavenger particle immunoassay
US4261968A (en) * 1979-05-10 1981-04-14 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4650770A (en) * 1981-04-27 1987-03-17 Syntex (U.S.A.) Inc. Energy absorbing particle quenching in light emitting competitive protein binding assays
US4713348A (en) * 1983-04-05 1987-12-15 Syntex (U.S.A.) Inc. Fluorescent multiparameter particle analysis
US5288609A (en) * 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
US4868104A (en) * 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US4996143A (en) * 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US5137827A (en) * 1986-03-25 1992-08-11 Midwest Research Technologies, Inc. Diagnostic element for electrical detection of a binding reaction
US5514602A (en) * 1986-06-09 1996-05-07 Ortho Diagnostic Systems, Inc. Method of producing a metal sol reagent containing colloidal metal particles
US5360895A (en) * 1987-04-22 1994-11-01 Associated Universities, Inc. Derivatized gold clusters and antibody-gold cluster conjugates
US4853335A (en) * 1987-09-28 1989-08-01 Olsen Duane A Colloidal gold particle concentration immunoassay
GB8800702D0 (en) 1988-01-13 1988-02-10 Nycomed As Test method & reagent kit therefor
WO1990002205A1 (en) 1988-08-25 1990-03-08 Angenics, Inc. Detection of nucleic acid sequences using particle agglutination
US5460831A (en) * 1990-06-22 1995-10-24 The Regents Of The University Of California Targeted transfection nanoparticles
GB9019512D0 (en) 1990-09-06 1990-10-24 Ici Plc Assay method
AU8951191A (en) * 1990-10-29 1992-05-26 Dekalb Plant Genetics Isolation of biological materials using magnetic particles
US5665582A (en) * 1990-10-29 1997-09-09 Dekalb Genetics Corp. Isolation of biological materials
US5294369A (en) * 1990-12-05 1994-03-15 Akzo N.V. Ligand gold bonding
EP0613585A4 (de) * 1991-11-22 1995-06-21 Univ California Halbleitende nanokristalle, die durch selbstzusammengesetzte einzelschichten mit festen anorganischen oberflüchen verbunden sind.
US5225064A (en) * 1992-01-15 1993-07-06 Enzyme Technology Research Group, Inc. Peroxidase colloidal gold oxidase biosensors for mediatorless glucose determination
GB9212164D0 (en) 1992-06-09 1992-07-22 Medical Res Council Preparation of nucleic acids
US5472881A (en) * 1992-11-12 1995-12-05 University Of Utah Research Foundation Thiol labeling of DNA for attachment to gold surfaces
US5637508A (en) * 1993-03-26 1997-06-10 Geo-Centers, Inc. Biomolecules bound to polymer or copolymer coated catalytic inorganic particles, immunoassays using the same and kits containing the same
US5384265A (en) * 1993-03-26 1995-01-24 Geo-Centers, Inc. Biomolecules bound to catalytic inorganic particles, immunoassays using the same
US5681943A (en) * 1993-04-12 1997-10-28 Northwestern University Method for covalently linking adjacent oligonucleotides
KR960703174A (ko) 1993-06-09 1996-06-19 미안 알렉 자기장 순환식 반응방법(magnetic cycle reaction)
EP0630974A3 (de) 1993-06-25 1995-11-15 Clinical Diagnostic Syst Verfahren und Testkit zum Nachweis von anorganischen Orthophosphat bei der Amplifizierung von Zielnukleinsäuren.
US5543158A (en) * 1993-07-23 1996-08-06 Massachusetts Institute Of Technology Biodegradable injectable nanoparticles
JPH07227299A (ja) 1994-02-14 1995-08-29 Kyoto Daiichi Kagaku:Kk Ｄｎａの特定塩基配列の検出方法及びその装置
US5521289A (en) * 1994-07-29 1996-05-28 Nanoprobes, Inc. Small organometallic probes
US5599668A (en) * 1994-09-22 1997-02-04 Abbott Laboratories Light scattering optical waveguide method for detecting specific binding events
US6025202A (en) * 1995-02-09 2000-02-15 The Penn State Research Foundation Self-assembled metal colloid monolayers and detection methods therewith
US5609907A (en) * 1995-02-09 1997-03-11 The Penn State Research Foundation Self-assembled metal colloid monolayers
CA2253710A1 (en) * 1996-04-25 1997-10-30 Spectrametrix Inc. Analyte assay using particulate labels
CA2262018C (en) * 1996-07-29 2007-10-02 Nanosphere Llc Nanoparticles having oligonucleotides attached thereto and uses therefor
US6582921B2 (en) 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US6361944B1 (en) * 1996-07-29 2002-03-26 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US7169556B2 (en) * 1996-07-29 2007-01-30 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6750016B2 (en) * 1996-07-29 2004-06-15 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6506564B1 (en) * 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6984491B2 (en) * 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US5830986A (en) * 1996-10-28 1998-11-03 Massachusetts Institute Of Technology Methods for the synthesis of functionalizable poly(ethylene oxide) star macromolecules
US5900481A (en) * 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
US5922537A (en) * 1996-11-08 1999-07-13 N.o slashed.AB Immunoassay, Inc. Nanoparticles biosensor
US5939021A (en) * 1997-01-23 1999-08-17 Hansen; W. Peter Homogeneous binding assay
US6974669B2 (en) * 2000-03-28 2005-12-13 Nanosphere, Inc. Bio-barcodes based on oligonucleotide-modified nanoparticles
US6149868A (en) * 1997-10-28 2000-11-21 The Penn State Research Foundation Surface enhanced raman scattering from metal nanoparticle-analyte-noble metal substrate sandwiches
JP2002509443A (ja) 1997-10-31 2002-03-26 ジェン−プローブ・インコーポレーテッド 核酸検出法
US5990479A (en) * 1997-11-25 1999-11-23 Regents Of The University Of California Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes
US5972615A (en) * 1998-01-21 1999-10-26 Urocor, Inc. Biomarkers and targets for diagnosis, prognosis and management of prostate disease
US6290839B1 (en) * 1998-06-23 2001-09-18 Clinical Micro Sensors, Inc. Systems for electrophoretic transport and detection of analytes
US6406921B1 (en) * 1998-07-14 2002-06-18 Zyomyx, Incorporated Protein arrays for high-throughput screening
US6251303B1 (en) * 1998-09-18 2001-06-26 Massachusetts Institute Of Technology Water-soluble fluorescent nanocrystals
US6306610B1 (en) * 1998-09-18 2001-10-23 Massachusetts Institute Of Technology Biological applications of quantum dots
US6203989B1 (en) * 1998-09-30 2001-03-20 Affymetrix, Inc. Methods and compositions for amplifying detectable signals in specific binding assays
US6277489B1 (en) * 1998-12-04 2001-08-21 The Regents Of The University Of California Support for high performance affinity chromatography and other uses
AU774593C (en) * 2000-01-13 2005-06-23 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
AU7687001A (en) * 2000-07-11 2002-01-21 Nanospherre Inc Method of detection by enhancement of silver staining
WO2002046483A2 (en) * 2000-12-06 2002-06-13 Northwestern University Silver stain removal from dna detection chips by cyanide etching or sonication
US20030113740A1 (en) * 2001-04-26 2003-06-19 Mirkin Chad A. Oligonucleotide-modified ROMP polymers and co-polymers
US7147687B2 (en) * 2001-05-25 2006-12-12 Nanosphere, Inc. Non-alloying core shell nanoparticles
US7238472B2 (en) * 2001-05-25 2007-07-03 Nanosphere, Inc. Non-alloying core shell nanoparticles
WO2003053535A2 (en) * 2001-08-03 2003-07-03 Nanosphere, Inc. Nanoparticle imaging system and method
DE60228128D1 (de) 2001-11-09 2008-09-18 Nanosphere Inc Biokonjugat-nanopartikelsonden
US20030211488A1 (en) * 2002-05-07 2003-11-13 Northwestern University Nanoparticle probs with Raman spectrocopic fingerprints for analyte detection
US6702411B2 (en) * 2002-06-21 2004-03-09 Oscar Helver Hands free cabinet drawer assist system
JP2005532456A (ja) 2002-07-02 2005-10-27 ナノスフェアー　インコーポレイテッド ナノ粒子ポリアニオン複合物ならびに分析物の検出における同複合物の用途

Also Published As

Publication number Publication date
EP1540006B1 (de) 2009-01-07
US7253277B2 (en) 2007-08-07
US20080153082A1 (en) 2008-06-26
WO2004004647A3 (en) 2004-03-25
EP1540006A2 (de) 2005-06-15
AU2003247788A1 (en) 2004-01-23
EP1540006A4 (de) 2006-08-09
AU2003247788B2 (en) 2007-04-19
CA2490413C (en) 2011-08-30
JP2005532456A (ja) 2005-10-27
WO2004004647A2 (en) 2004-01-15
ATE420357T1 (de) 2009-01-15
CA2490413A1 (en) 2004-01-15
US20040053222A1 (en) 2004-03-18

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