DE69017204T2 - Immobilisierte oligonukleotidsonden und ihre verwendungen. - Google Patents

Immobilisierte oligonukleotidsonden und ihre verwendungen.

Info

Publication number
DE69017204T2
DE69017204T2 DE69017204T DE69017204T DE69017204T2 DE 69017204 T2 DE69017204 T2 DE 69017204T2 DE 69017204 T DE69017204 T DE 69017204T DE 69017204 T DE69017204 T DE 69017204T DE 69017204 T2 DE69017204 T2 DE 69017204T2
Authority
DE
Germany
Prior art keywords
polynucleotide
nucleic acids
samples
run
oligonucleotide probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69017204T
Other languages
English (en)
Other versions
DE69017204D1 (de
Inventor
Mark Collins
David Morrissey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BP Corp North America Inc
Original Assignee
BP Corp North America Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BP Corp North America Inc filed Critical BP Corp North America Inc
Application granted granted Critical
Publication of DE69017204D1 publication Critical patent/DE69017204D1/de
Publication of DE69017204T2 publication Critical patent/DE69017204T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00504—Pins
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00511—Walls of reactor vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69017204T 1989-03-10 1990-03-06 Immobilisierte oligonukleotidsonden und ihre verwendungen. Expired - Fee Related DE69017204T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32172889A 1989-03-10 1989-03-10
PCT/US1990/001205 WO1990010716A1 (en) 1989-03-10 1990-03-06 Immobilized oligonucleotide probes and uses therefor

Publications (2)

Publication Number Publication Date
DE69017204D1 DE69017204D1 (de) 1995-03-30
DE69017204T2 true DE69017204T2 (de) 1995-08-03

Family

ID=23251773

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69017204T Expired - Fee Related DE69017204T2 (de) 1989-03-10 1990-03-06 Immobilisierte oligonukleotidsonden und ihre verwendungen.

Country Status (8)

Country Link
US (2) US6268128B1 (de)
EP (1) EP0463036B1 (de)
JP (1) JP3046837B2 (de)
AT (1) ATE118824T1 (de)
AU (1) AU5269590A (de)
CA (1) CA2049043A1 (de)
DE (1) DE69017204T2 (de)
WO (1) WO1990010716A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10328624A1 (de) * 2003-06-25 2005-01-20 Advalytix Ag Verfahren zur Hybridisierung

Families Citing this family (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2028012A1 (en) * 1989-10-23 1991-04-24 Randall Dimond Hybridization assay for campylobacter rrna
AU640745B2 (en) * 1990-03-30 1993-09-02 Astra Aktiebolag A novel procedure for the detection of pathogens using dna probes
IL99025A0 (en) * 1990-08-15 1992-07-15 Astra Ab Novel process for the detection of pathogens using dna probes
EP0529070A1 (de) * 1991-02-27 1993-03-03 Amoco Corporation Verfahren zur verbesserung der empfindlichkeit von hybridisierungsassays
DE69232753T2 (de) * 1991-11-01 2003-05-15 Diatech Pty Ltd Feststoff-phase erweiterungsverfahren
JPH08500722A (ja) * 1992-01-29 1996-01-30 日立化成工業株式会社 ポリヌクレオチド固定化担体
US5849544A (en) * 1992-07-24 1998-12-15 University Of Australia Amplification and detection process
AU698934B2 (en) * 1992-07-24 1998-11-12 Eppendorf Ag Amplification and detection process
EP0625213B1 (de) * 1992-10-09 1999-12-29 VYSIS, Inc. Analytisches verfahren
RU2041262C1 (ru) * 1993-08-11 1995-08-09 Институт молекулярной биологии им.В.А.Энгельгардта РАН Способ иммобилизации водорастворимых биоорганических соединений на капиллярно-пористый носитель
US20120329677A9 (en) * 1993-10-26 2012-12-27 Cronin Maureen T Arrays of nucleic acid probes for detecting cystic fibrosis
US6893816B1 (en) 1993-10-28 2005-05-17 Houston Advanced Research Center Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions
US5646263A (en) * 1994-09-19 1997-07-08 Promega Corporation High efficiency method for isolating target substances using a multisample separation device
US5693784A (en) * 1994-09-19 1997-12-02 Promega Corporation Methods for creating agglomerates from colloidal particles
US5567326A (en) * 1994-09-19 1996-10-22 Promega Corporation Multisample magnetic separation device
FR2728264B1 (fr) 1994-12-16 1997-01-31 Rhone Poulenc Rorer Sa Purification d'adn par formation de triple helice avec un oligonucleotide immobilise
US7038026B2 (en) 2000-05-26 2006-05-02 Centelion Purification of a triple heli formation with an immobilized oligonucleotide
DE19526431A1 (de) * 1995-07-21 1997-01-23 Boehringer Mannheim Gmbh Verfahren und Reagenz zur spezifischen Bestimmung von mRNA
US6391550B1 (en) 1996-09-19 2002-05-21 Affymetrix, Inc. Identification of molecular sequence signatures and methods involving the same
IL124275A (en) * 1997-05-02 2002-03-10 Bio Merieux Vitek Inc A method to produce sequences of nucleic acids
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
WO1998050583A1 (en) 1997-05-02 1998-11-12 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US6558901B1 (en) * 1997-05-02 2003-05-06 Biomerieux Vitek Nucleic acid assays
ES2246546T3 (es) * 1997-12-12 2006-02-16 Digene Corporation Valoracion de enfermedades relacionadas con el virus de papiloma humano.
US6077673A (en) * 1998-03-31 2000-06-20 Clontech Laboratories, Inc. Mouse arrays and kits comprising the same
US6087112A (en) 1998-12-30 2000-07-11 Oligos Etc. Inc. Arrays with modified oligonucleotide and polynucleotide compositions
US6544777B1 (en) * 1999-06-03 2003-04-08 Jacques Schrenzel Non-cognate hybridization system (NCHS)
US6346423B1 (en) 1999-07-16 2002-02-12 Agilent Technologies, Inc. Methods and compositions for producing biopolymeric arrays
US6844151B1 (en) 1999-09-29 2005-01-18 Oligos Etc. Inc. Methods for production of arrays with modified oligonucleotide and polynucleotide compositions
US7109024B2 (en) * 1999-11-15 2006-09-19 Dr. Chip Biotechnology Inc. Biomolecule-bound substrates
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7439016B1 (en) * 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
DE10126630A1 (de) * 2001-05-31 2003-01-09 Peter Und Traudl Engelhorn Sti Verfahren zur Zellsortierung
EP1444364A4 (de) * 2001-10-27 2006-03-15 Sigma Genosys Inc Multiple oligonukleotide pro gen für die anwendung bei gen-arrays
US7508608B2 (en) 2004-11-17 2009-03-24 Illumina, Inc. Lithographically fabricated holographic optical identification element
US7901630B2 (en) 2002-08-20 2011-03-08 Illumina, Inc. Diffraction grating-based encoded microparticle assay stick
US7900836B2 (en) 2002-08-20 2011-03-08 Illumina, Inc. Optical reader system for substrates having an optically readable code
WO2004019277A1 (en) * 2002-08-20 2004-03-04 Cyvera Corporation Diffraction grating-based encoded micro-particles for multiplexed experiments
AU2003265583C1 (en) * 2002-08-20 2009-05-21 Cyvera Corporation Diffraction grating-based optical identification element
US7923260B2 (en) 2002-08-20 2011-04-12 Illumina, Inc. Method of reading encoded particles
US7164533B2 (en) 2003-01-22 2007-01-16 Cyvera Corporation Hybrid random bead/chip based microarray
US7872804B2 (en) 2002-08-20 2011-01-18 Illumina, Inc. Encoded particle having a grating with variations in the refractive index
CA2498916A1 (en) * 2002-09-12 2004-03-25 Cyvera Corporation Chemical synthesis using diffraction grating-based encoded optical elements
US7092160B2 (en) 2002-09-12 2006-08-15 Illumina, Inc. Method of manufacturing of diffraction grating-based optical identification element
US20100255603A9 (en) 2002-09-12 2010-10-07 Putnam Martin A Method and apparatus for aligning microbeads in order to interrogate the same
CA2498913A1 (en) * 2002-09-12 2004-03-25 Cyvera Corporation Assay stick comprising coded microbeads
BRPI0408967B8 (pt) 2003-03-31 2021-07-27 Hoffmann La Roche kit e métodos para detecção de um ácido nucléico de vários membros do sorogrupo do vírus da encefalite japonesa em uma amostra biológica sob condições de hibridização rigorosas
US7920906B2 (en) 2005-03-10 2011-04-05 Dexcom, Inc. System and methods for processing analyte sensor data for sensor calibration
US20050059083A1 (en) * 2003-09-15 2005-03-17 Becton Dickinson And Company High throughput method to identify ligands for cell attachment
US7279280B2 (en) * 2003-09-25 2007-10-09 Mgp Biotech, Inc. Apparatus and method for detecting genetic mutations and single nucleotide polymorphisms
US9247900B2 (en) 2004-07-13 2016-02-02 Dexcom, Inc. Analyte sensor
US7433123B2 (en) 2004-02-19 2008-10-07 Illumina, Inc. Optical identification element having non-waveguide photosensitive substrate with diffraction grating therein
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
DE602005020943D1 (de) 2004-07-01 2010-06-10 Gen Probe Inc Verfahren und zusammensetzungen zum nachweis von nukleinsäuren in einer biologischen probe
US7654956B2 (en) 2004-07-13 2010-02-02 Dexcom, Inc. Transcutaneous analyte sensor
WO2006127694A2 (en) 2004-07-13 2006-11-30 Dexcom, Inc. Analyte sensor
WO2006020363A2 (en) 2004-07-21 2006-02-23 Illumina, Inc. Method and apparatus for drug product tracking using encoded optical identification elements
DE602005019791D1 (de) 2004-11-16 2010-04-15 Illumina Inc Verfahren und vorrichtung zum lesen von kodierten mikrokugeln
WO2006095941A1 (en) 2005-03-05 2006-09-14 Seegene, Inc. Processes using dual specificity oligonucleotide and dual specificity oligonucleotide
KR100977186B1 (ko) 2005-03-05 2010-08-23 주식회사 씨젠 이중 특이성 올리고뉴클레오타이드를 사용한 방법 및 이중특이성 올리고뉴클레오타이드
US7830575B2 (en) 2006-04-10 2010-11-09 Illumina, Inc. Optical scanner with improved scan time
JP2011518333A (ja) * 2008-04-17 2011-06-23 キアジェン ゲイサーズバーグ インコーポレイテッド 標的核酸の存在を判別するための合成プローブを用いた組成物、方法、およびキット
EP2806037B1 (de) * 2008-05-13 2016-09-21 Gen-Probe Incorporated Inaktivierbare Target-Capture-Oligomere zur Verwendung bei der selektiven Hybridisierung und beim Fangen von Zielnukleinsäuresequenzen
US8288520B2 (en) * 2008-10-27 2012-10-16 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and system
AU2009313808B2 (en) 2008-11-14 2013-05-16 Gen-Probe Incorporated Compositions, kits and methods for detection of campylobacter nucleic acid
EP2367958B1 (de) * 2008-11-25 2017-08-23 Gen-Probe Incorporated ZUSAMMENSETZUNGEN UND VERFAHREN ZUR ERKENNUNG VON sRNA UND IHRE VERWENDUNG
ES2644516T3 (es) * 2009-01-28 2017-11-29 Qiagen Gaithersburg, Inc. Método y ensayo de preparación de muestras de gran volumen específico de secuencia
WO2010088517A1 (en) * 2009-01-30 2010-08-05 The U.S.A., As Represented By The Secretary, Department Of Health And Human Services Methods and systems for purifying transferring and/or manipulating nucleic acids
US9797000B2 (en) * 2009-05-01 2017-10-24 Qiagen Gaithersburg Inc. Non-target amplification method for detection of RNA splice-forms in a sample
WO2011027966A2 (en) 2009-09-03 2011-03-10 Seegene, Inc. Td probe and its uses
EP2478087B1 (de) 2009-09-14 2017-01-18 QIAGEN Gaithersburg, Inc. Zusammensetzungen und verfahren zur rückgewinnung von nukleinsäuren oder proteinen aus in zytologischen medien fixierten gewebeproben
JP5623888B2 (ja) * 2009-12-10 2014-11-12 エフ．ホフマン−ラ ロシュアーゲーＦ．Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 分析対象物を分離して検出する方法
JP2013517803A (ja) 2010-01-29 2013-05-20 キアジェン ゲイサーズバーグ インコーポレイテッド サンプル中のｈｐｖの存在を決定および確認する方法
CN102822189B (zh) 2010-01-29 2016-06-29 奇亚根盖瑟斯堡股份有限公司 用于核酸的序列特异性纯化和多重复合体分析(multiplex analysis)的方法和组合物
JP2013528049A (ja) 2010-05-19 2013-07-08 キアゲン ガイサーズバーグ アイエヌシー． 核酸の配列特異的な精製及び多重分析のための方法及び組成物
AU2011258501B2 (en) 2010-05-25 2016-07-07 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically-truncated probes
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
US9885092B2 (en) 2011-02-24 2018-02-06 Qiagen Gaithersburg Inc. Materials and methods for detection of HPV nucleic acids
AT512416B1 (de) 2012-02-13 2013-10-15 Greiner Bio One Gmbh Anordnung und verfahren zum nachweis von mikroorganismen in einem kulturgefäss
US9651549B2 (en) * 2012-07-13 2017-05-16 Genisphere, Llc Lateral flow assays using DNA dendrimers
MY195756A (en) 2012-09-17 2023-02-09 Grace W R & Co Functionalized Particulate Support Material and Methods of Making and Using the Same
CA2885263C (en) 2012-09-17 2021-11-16 W. R. Grace & Co.-Conn. Chromatography media and devices
JP6853670B2 (ja) 2014-01-16 2021-04-07 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ 親和性クロマトグラフィー媒体及びクロマトグラフィーデバイス
WO2015168383A1 (en) 2014-05-02 2015-11-05 W. R. Grace & Co.-Conn. Functionalized support material and methods of making and using functionalized support material
SG10201911134QA (en) 2015-06-05 2020-01-30 Grace W R & Co Adsorbent bioprocessing clarification agents and methods of making and using the same
DE102018103215B3 (de) 2018-02-13 2019-08-14 Gna Biosolutions Gmbh Verfahren und Vorrichtung zur Extraktion einer Nukleinsäure aus einer Probenflüssigkeit

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4358535A (en) * 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
NO843838L (no) * 1983-09-26 1985-03-27 Ortho Diagnostic Systems Inc Fremgangsmaate for detektering av nukleinsyre
US5288609A (en) 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
FI72146C (fi) 1985-01-02 1987-04-13 Orion Yhtymae Oy Foerfarande foer identifiering av nukleinsyror.
US4753775A (en) * 1985-04-12 1988-06-28 E. I. Du Pont De Nemours And Company Rapid assay processor
US4767699A (en) * 1985-05-02 1988-08-30 Allied Corporation Diagnostic reagent, kit and method employing polynucleotide displacement, separation, enzymatic cleavage and adenosine phosphate detection
US4797355A (en) 1985-06-13 1989-01-10 Amgen Inc. Methods for attaching polynucleotides to supports
US4751177A (en) * 1985-06-13 1988-06-14 Amgen Methods and kits for performing nucleic acid hybridization assays
AU6834187A (en) * 1985-12-17 1987-07-15 Genetics Institute Inc. Displacement polynucleotide method and reagent complex
ATE88761T1 (de) 1986-01-10 1993-05-15 Amoco Corp Kompetitiver homogener test.
ZA877772B (en) 1986-10-23 1988-04-20 Amoco Corporation Target and background capture methods and apparatus for affinity assays
EP0305145A3 (de) * 1987-08-24 1990-05-02 Ortho Diagnostic Systems Inc. Verfahren und Sonden zur Bestimmung von Nukleinsäuren
CA2049042A1 (en) * 1989-03-10 1990-09-11 Mark L. Collins Preventing interference with affinity capture schemes

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10328624A1 (de) * 2003-06-25 2005-01-20 Advalytix Ag Verfahren zur Hybridisierung

Also Published As

Publication number Publication date
EP0463036A1 (de) 1992-01-02
WO1990010716A1 (en) 1990-09-20
JPH04504053A (ja) 1992-07-23
JP3046837B2 (ja) 2000-05-29
ATE118824T1 (de) 1995-03-15
AU5269590A (en) 1990-10-09
EP0463036B1 (de) 1995-02-22
US20020001811A1 (en) 2002-01-03
CA2049043A1 (en) 1990-09-11
DE69017204D1 (de) 1995-03-30
US6268128B1 (en) 2001-07-31

Similar Documents

Publication Publication Date Title
DE69017204T2 (de) Immobilisierte oligonukleotidsonden und ihre verwendungen.
DE69928747D1 (de) Verfahen und Zusammensetzungen zur Vermehrung von feststellbaren Signalen in spezifischen Bindungstests
ATE66695T1 (de) Verfahren zur erhoehung der empfindlichkeit von nukleinsaeure-hybridisierungstesten.
NO852022L (no) Nukleinsyre-hybridiseringsanalyse ved anvendelse av detekterbare anti-hybrid-antistoffer.
DE60128908D1 (de) Verfahren zum Nachweis und zur Lokalisierung von Genen in situ durch Hybridisierung einer verzweigten DNA
NO860400L (no) Dual hybridiseringsanalyse i opploesningsfase for detektering av polynukleotide sekvenser.
ATE133995T1 (de) Verfahren zum nukleotidsequenzennachweis mittels technik der sandwich-hybridisierung
DK463089D0 (da) Fremgangsmaade og reagenssaet til detektion af en specifik nucleinsyresekvens i en proeve indeholdende en nucleinsyre
AU1010688A (en) Diagnostic assays using nucleic acid probes
DE69807293D1 (de) Multiplex-PCR zum Nachweis von Infektionen verursacht durch Mycobacterium Avium Komplex
DE68919706T2 (de) Verfahren zum schnellen nachweis von nukleinsäuren.
ES8801375A1 (es) Un metodo para determinar una secuencia polinucleotidica particular presente en un virus o celula
CN107828863B (zh) 一种用于rca扩增产物的快速银染色方法
WO2001053817A3 (en) Methods of and apparatus for separating and detecting nucleic acid
CN116837075A (zh) 一种基于固相载体的核酸检测装置及方法
IL73578A (en) Method and reagent for the detection of polynucleotide sequence in sample of dna or rna by using a nucleic acid probe and contact of duplexes with immobilizing antibody

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee