DE69109109D1 - Ubiquitinspezifische protease. - Google Patents

Ubiquitinspezifische protease.

Info

Publication number
DE69109109D1
DE69109109D1 DE69109109T DE69109109T DE69109109D1 DE 69109109 D1 DE69109109 D1 DE 69109109D1 DE 69109109 T DE69109109 T DE 69109109T DE 69109109 T DE69109109 T DE 69109109T DE 69109109 D1 DE69109109 D1 DE 69109109D1
Authority
DE
Germany
Prior art keywords
ubiquitin
peptide
protease
protein
moiety
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69109109T
Other languages
English (en)
Other versions
DE69109109T2 (de
Inventor
Alexander Varshavsky
John Tobias
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Massachusetts Institute of Technology
Original Assignee
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Institute of Technology filed Critical Massachusetts Institute of Technology
Publication of DE69109109D1 publication Critical patent/DE69109109D1/de
Application granted granted Critical
Publication of DE69109109T2 publication Critical patent/DE69109109T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)
DE69109109T 1990-05-09 1991-05-08 Ubiquitinspezifische protease. Expired - Fee Related DE69109109T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US52108990A 1990-05-09 1990-05-09
US57395890A 1990-08-28 1990-08-28
PCT/US1991/003177 WO1991017245A1 (en) 1990-05-09 1991-05-08 Ubiquitin-specific protease

Publications (2)

Publication Number Publication Date
DE69109109D1 true DE69109109D1 (de) 1995-05-24
DE69109109T2 DE69109109T2 (de) 1995-09-14

Family

ID=27060368

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69109109T Expired - Fee Related DE69109109T2 (de) 1990-05-09 1991-05-08 Ubiquitinspezifische protease.

Country Status (9)

Country Link
US (1) US5391490A (de)
EP (1) EP0531404B1 (de)
JP (1) JP3041644B2 (de)
AT (1) ATE121454T1 (de)
CA (1) CA2079229C (de)
DE (1) DE69109109T2 (de)
DK (1) DK0531404T3 (de)
ES (1) ES2071315T3 (de)
WO (1) WO1991017245A1 (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5212058A (en) * 1990-05-09 1993-05-18 Massachusetts Institute Of Technology Nucleic acid encoding ubiquitin-specific proteases
US5981699A (en) * 1994-01-04 1999-11-09 Mitotix, Inc. Human ubiquitin conjugating enzyme
WO1995018974A2 (en) * 1994-01-04 1995-07-13 Mitotix, Inc. Ubiquitin conjugating enzymes
US5744343A (en) * 1994-01-04 1998-04-28 Mitotix, Inc. Ubiquitin conjugating enzymes
AU7357896A (en) 1995-08-09 1997-03-05 Dana-Farber Cancer Institute Deubiquitinating enzymes that regulate cell growth
US5770720A (en) * 1995-08-30 1998-06-23 Barnes-Jewish Hospital Ubiquitin conjugating enzymes having transcriptional repressor activity
US6001619A (en) * 1995-10-04 1999-12-14 Cold Spring Harbor Laboratory Ubiquitin ligases, and uses related thereto
US5861483A (en) * 1996-04-03 1999-01-19 Pro-Neuron, Inc. Inhibitor of stem cell proliferation and uses thereof
GB9618960D0 (en) 1996-09-11 1996-10-23 Medical Science Sys Inc Proteases
US6747128B2 (en) 1997-08-20 2004-06-08 Gpc Biotech, Inc. Components of ubiquitin ligase complexes, and uses related thereto
US5932422A (en) * 1997-11-14 1999-08-03 Millennium Pharmaceuticals, Inc. Modulation of drug resistance via ubiquitin carboxy-terminal hydrolase
US7668658B2 (en) * 1999-10-13 2010-02-23 Sequenom, Inc. Methods for generating databases and databases for identifying polymorphic genetic markers
WO2001066763A2 (en) * 2000-03-07 2001-09-13 Millennium Pharmaceuticals, Inc. 23436, a human ubiquitin protease family member and uses thereof
US7070947B2 (en) * 2000-02-29 2006-07-04 Millennium Pharmaceuticals, Inc. Human protein kinase, phosphatase, and protease family members and uses thereof
US7186528B2 (en) 2000-06-15 2007-03-06 Smithkline Beecham Corporation Method for preparing a physiologically active IL-18 polypeptide
EP1294905A1 (de) * 2000-06-26 2003-03-26 SmithKline Beecham Biologics SA Triple-fusionsprotein von ubiquitin, die zwischen thioredoxin und ein polypeptid von interesse fusioniert ist
US7122332B2 (en) * 2001-03-29 2006-10-17 Rigel Pharmaceuticals, Inc. Modulators of leukocyte activation, compositions and methods of use
AU2003298733B2 (en) * 2002-11-27 2009-06-18 Agena Bioscience, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
EP1618216A2 (de) * 2003-04-25 2006-01-25 Sequenom, Inc. Auf fragmentierung beruhende verfahren und systeme zur de-novo-sequenzierung
US9394565B2 (en) * 2003-09-05 2016-07-19 Agena Bioscience, Inc. Allele-specific sequence variation analysis
US7608394B2 (en) 2004-03-26 2009-10-27 Sequenom, Inc. Methods and compositions for phenotype identification based on nucleic acid methylation
US9249456B2 (en) 2004-03-26 2016-02-02 Agena Bioscience, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
US20060073501A1 (en) * 2004-09-10 2006-04-06 Van Den Boom Dirk J Methods for long-range sequence analysis of nucleic acids
PL1841862T3 (pl) 2005-01-10 2013-10-31 Inst Biotechnologii I Antybiotykow Mutant proteazy UBP1 i jego sekwencja kodująca, ich zastosowania oraz system ekspresji białka heterologicznego
US8173792B2 (en) * 2007-02-09 2012-05-08 The Board Of Trustees Of The Leland Stanford Junior University Method for regulating protein function in cells using synthetic small molecules
CA2684217C (en) * 2007-04-13 2016-12-13 Sequenom, Inc. Comparative sequence analysis processes and systems
US8530636B2 (en) * 2008-05-07 2013-09-10 The Board Of Trustees Of The Leland Stanford Junior University Method for regulating protein function in cells in vivo using synthetic small molecules
WO2019173462A1 (en) * 2018-03-06 2019-09-12 Pepvax, Inc. Nucleic acid molecules and methods of using the same

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0324789B1 (de) * 1986-10-02 2003-08-20 Massachusetts Institute Of Technology Verfahren zur regulierung der metabolischen stabilität von proteinen
US5108919A (en) * 1988-06-24 1992-04-28 Genentech, Inc. Dna sequences encoding yeast ubiquitin hydrolase

Also Published As

Publication number Publication date
CA2079229C (en) 1999-09-14
JP3041644B2 (ja) 2000-05-15
WO1991017245A1 (en) 1991-11-14
ATE121454T1 (de) 1995-05-15
JPH06500006A (ja) 1994-01-06
DK0531404T3 (da) 1995-09-18
EP0531404A1 (de) 1993-03-17
ES2071315T3 (es) 1995-06-16
US5391490A (en) 1995-02-21
EP0531404B1 (de) 1995-04-19
CA2079229A1 (en) 1991-11-10
DE69109109T2 (de) 1995-09-14

Similar Documents

Publication Publication Date Title
DK0531404T3 (da) Ubiquitin-specifik protease
Carter et al. Engineering subtilisin BPN′ for site‐specific proteolysis
Inouye Intramolecular chaperone: the role of the pro-peptide in protein folding
ATE68014T1 (de) Die synthese eines proteins mittels eines identifizierungspeptids.
Ugai et al. Purification and characterization of the 26S proteasome complex catalyzing ATP-dependent breakdown of ubiquitin-ligated prot from rat liver
NZ232742A (en) Yeast heterologous protein production method, vectors, dna constructs and proteins so produced and aprotinin (1-58) so produced
ATE272109T1 (de) Rekombinante collagenase typ i aus clostridium histolyticum und ihre verwendung zur isolierung von zellen und zellverbänden
AU6132294A (en) Fusion proteins including a cleavage site recognized by a plant virus protease
ZA8860B (en) Pancreatic secretory trypsin inhibitor and variants thereof produced by a recombinant host,process,expression vector and recombinant host therefor and pharmaceutical use thereof
KR940703860A (ko) 신규한 사람 쿠니즈형 프로테아제 저해제 및 그의 변이체(a novel human kunitz-type protease inhibitor and variants thereof)
EP0528686B8 (de) Verfahren zur Herstellung von Peptiden
EP0195680A3 (de) Proteinsynthese mit einem Identifizierungspeptid
ATE317443T1 (de) N-terminal verlängerte proteine exprimiert in hefe
IL90947A (en) Method for production of hiv protease in transformed cells, dna and vectors comprising said dna used in said production
KR940009329A (ko) 딕티오스텔륨 디펩티딜아미노펩티다아제
Ohkubo et al. Molecular cloning and immunohistochemical localization of rat dipeptidyl peptidase III
Harris et al. Amino acid sequences in penicillo-pepsin: evidence for homology with porcine pepsin and chymosin.
ATE309263T1 (de) Reinigungsverfahren auf grundlage der bindung von enzymen and markierte peptide
WO2001047958A3 (en) Kunitz domain polypeptide zkun10
Pal et al. Crystallization of an enzyme‐inhibitor complex: Proteinase K with its protein inhibitor, PK13
TH17635A (th) อะมิโนเพพติเดสชนิดใหม่ กระบวนการเตรียมอะมิโนเพพติเดสและโปรตีนจากมัน
TH17635EX (th) อะมิโนเพพติเดสชนิดใหม่ กระบวนการเตรียมอะมิโนเพพติเดสและโปรตีนจากมัน
KR20150123455A (ko) 효모를 이용한 티모신 베타4의 생산방법
JO2429B1 (en) Ways to produce recombinant peptides of antigen - merger

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee