DE69122457D1 - Verfahren zur Herstellung eines Polynukleotides zur Verwendung bei Einzelprimeramplifikation - Google Patents
Verfahren zur Herstellung eines Polynukleotides zur Verwendung bei EinzelprimeramplifikationInfo
- Publication number
- DE69122457D1 DE69122457D1 DE69122457T DE69122457T DE69122457D1 DE 69122457 D1 DE69122457 D1 DE 69122457D1 DE 69122457 T DE69122457 T DE 69122457T DE 69122457 T DE69122457 T DE 69122457T DE 69122457 D1 DE69122457 D1 DE 69122457D1
- Authority
- DE
- Germany
- Prior art keywords
- extended
- primer
- polynucleotide
- extender probe
- duplex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/555,323 US5595891A (en) | 1990-07-19 | 1990-07-19 | Method for producing a polynucleotide for use in single primer amplification |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69122457D1 true DE69122457D1 (de) | 1996-11-07 |
DE69122457T2 DE69122457T2 (de) | 1997-03-20 |
Family
ID=24216831
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69122457T Expired - Fee Related DE69122457T2 (de) | 1990-07-19 | 1991-07-18 | Verfahren zur Herstellung eines Polynukleotides zur Verwendung bei Einzelprimeramplifikation |
Country Status (7)
Country | Link |
---|---|
US (1) | US5595891A (de) |
EP (1) | EP0469755B1 (de) |
JP (1) | JPH0584079A (de) |
AT (1) | ATE143697T1 (de) |
CA (1) | CA2047342A1 (de) |
DE (1) | DE69122457T2 (de) |
ES (1) | ES2091876T3 (de) |
Families Citing this family (52)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5525462A (en) * | 1991-05-02 | 1996-06-11 | Toyo Boseki Kabushiki Kaisha | Nucleic acid sequence amplification method, detection method, and reagent kit therefor |
ATE161586T1 (de) | 1991-10-11 | 1998-01-15 | Behringwerke Ag | Verfahren zur herstellung eines polynukleotids zum gebrauch in ''single-primer'' amplifizierung und phosphorothioat-enthaltende oligonukleotide als primer in nukleinsäureamplifizierung |
ZA929319B (en) * | 1991-12-11 | 1993-05-24 | Igen Inc | Method for exponential amplification of nucleic acid by a single unpaired primer. |
EP1382386A3 (de) * | 1992-02-19 | 2004-12-01 | The Public Health Research Institute Of The City Of New York, Inc. | Neue Ordnungen für Oligonukleotide und ihr Nutzen zum Sortieren, Isolieren, Sequenzierung und Manipulieren von Nukleinsäuren |
GB9210176D0 (en) * | 1992-05-12 | 1992-06-24 | Cemu Bioteknik Ab | Chemical method |
GB9210177D0 (en) * | 1992-05-12 | 1992-06-24 | Cemu Bioteknik Ab | Loop structures |
ATE152180T1 (de) * | 1992-07-31 | 1997-05-15 | Behringwerke Ag | Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden |
WO1994003624A1 (en) * | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
US6261808B1 (en) | 1992-08-04 | 2001-07-17 | Replicon, Inc. | Amplification of nucleic acid molecules via circular replicons |
US6294323B1 (en) * | 1993-04-14 | 2001-09-25 | Behringwerke Ag | Self initiating single primer amplification of nucleic acids |
US5470723A (en) * | 1993-05-05 | 1995-11-28 | Becton, Dickinson And Company | Detection of mycobacteria by multiplex nucleic acid amplification |
US5422252A (en) * | 1993-06-04 | 1995-06-06 | Becton, Dickinson And Company | Simultaneous amplification of multiple targets |
US5681697A (en) * | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
US5710000A (en) * | 1994-09-16 | 1998-01-20 | Affymetrix, Inc. | Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping |
WO1997004131A1 (en) * | 1995-07-21 | 1997-02-06 | Forsyth Dental Infirmary For Children | Single primer amplification of polynucleotide hairpins |
EP0873422B1 (de) * | 1995-11-25 | 2007-01-10 | Roche Diagnostics GmbH | Verfahren zur herstellung vielfältiger doppelsträngiger nukleinsäuren |
DE69633941T2 (de) * | 1995-12-22 | 2005-11-24 | Dade Behring Marburg Gmbh | Nachweis von unterschieden in nukleinsäuren |
US6326143B1 (en) | 1996-11-22 | 2001-12-04 | Roche Diagnostics Gmbh | Method for generating multiple double stranded nucleic acids |
US8445664B2 (en) * | 1998-06-24 | 2013-05-21 | Enzo Diagnostics, Inc. | Kits for amplifying and detecting nucleic acid sequences |
US6743605B1 (en) * | 1998-06-24 | 2004-06-01 | Enzo Life Sciences, Inc. | Linear amplification of specific nucleic acid sequences |
EP2287338B1 (de) | 1998-11-09 | 2012-09-05 | Eiken Kagaku Kabushiki Kaisha | Prozess zur Synthetisierung von Nukleinsäure |
US6232104B1 (en) | 1999-08-17 | 2001-05-15 | Dade Behring Inc. | Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration |
CA2384838C (en) | 1999-09-13 | 2006-07-18 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US6692918B2 (en) | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
CN1415020A (zh) | 1999-11-08 | 2003-04-30 | 荣研化学株式会社 | 检测突变和/或多态性的方法 |
JP4721603B2 (ja) * | 1999-11-08 | 2011-07-13 | 栄研化学株式会社 | 変異および/または多型の検出方法 |
CN100422323C (zh) * | 2000-04-07 | 2008-10-01 | 荣研化学株式会社 | 使用双链核酸为模板扩增核酸的方法 |
US7846733B2 (en) * | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
WO2002000938A2 (en) * | 2000-06-26 | 2002-01-03 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
US6919189B2 (en) | 2000-12-11 | 2005-07-19 | Alexion Pharmaceuticals, Inc. | Nested oligonucleotides containing a hairpin for nucleic acid amplification |
WO2002048402A2 (en) * | 2000-12-13 | 2002-06-20 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
ZA200210369B (en) | 2001-03-09 | 2004-07-08 | Nugen Technologies Inc | Methods and compositions for amplification or RNA sequences. |
US7094536B2 (en) * | 2001-03-09 | 2006-08-22 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences |
US7414111B2 (en) * | 2001-09-19 | 2008-08-19 | Alexion Pharmaceuticals, Inc. | Engineered templates and their use in single primer amplification |
DE60234369D1 (de) * | 2001-09-19 | 2009-12-24 | Alexion Pharma Inc | Manipulierte matrizen und ihre verwendung bei der single-primer-amplifikation |
US7045319B2 (en) * | 2001-10-30 | 2006-05-16 | Ribomed Biotechnologies, Inc. | Molecular detection systems utilizing reiterative oligonucleotide synthesis |
US20040054162A1 (en) * | 2001-10-30 | 2004-03-18 | Hanna Michelle M. | Molecular detection systems utilizing reiterative oligonucleotide synthesis |
US20110151438A9 (en) | 2001-11-19 | 2011-06-23 | Affymetrix, Inc. | Methods of Analysis of Methylation |
AU2002366098A1 (en) * | 2001-11-19 | 2003-06-10 | Parallele Bioscience Inc | Multiplex pcr |
GB0218215D0 (en) * | 2002-08-06 | 2002-09-11 | Tepnel Medical Ltd | Amplification of nucleic acids |
WO2004092418A2 (en) * | 2003-04-14 | 2004-10-28 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
CN101068934A (zh) * | 2003-10-29 | 2007-11-07 | 里伯米德生物技术公司 | 用于反复合成寡核苷酸的组合物、方法及检测技术 |
CA2621267A1 (en) | 2005-09-07 | 2007-03-15 | Nugen Technologies, Inc. | Improved nucleic acid amplification procedure |
US8137936B2 (en) * | 2005-11-29 | 2012-03-20 | Macevicz Stephen C | Selected amplification of polynucleotides |
US8034568B2 (en) * | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
GB2470672B (en) | 2008-03-21 | 2012-09-12 | Nugen Technologies Inc | Methods of RNA amplification in the presence of DNA |
CA2724343A1 (en) | 2008-05-15 | 2009-11-19 | Ribomed Biotechnologies, Inc. | Methods and reagents for detecting cpg methylation with a methyl cpg binding protein (mbp) |
US8211644B2 (en) * | 2008-07-13 | 2012-07-03 | Ribomed Biotechnologies, Inc. | Molecular beacon-based methods for detection of targets using abscription |
JP2012520086A (ja) | 2009-03-15 | 2012-09-06 | リボムド バイオテクノロジーズ インク | アブスクリプションに基づく分子検出 |
CA2686250C (en) | 2009-11-12 | 2016-10-11 | Fountainhead, Llc | Floating treatment streambed |
WO2014085687A1 (en) | 2012-11-28 | 2014-06-05 | Abwiz Bio, Inc. | Preparation of gene-specific templates for the use in single primer amplification |
US10694693B2 (en) | 2015-12-18 | 2020-06-30 | Monsanto Technology Llc | Methods for producing corn plants with northern leaf blight resistance and compositions thereof |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4868104A (en) * | 1985-09-06 | 1989-09-19 | Syntex (U.S.A.) Inc. | Homogeneous assay for specific polynucleotides |
US5066584A (en) * | 1988-09-23 | 1991-11-19 | Cetus Corporation | Methods for generating single stranded dna by the polymerase chain reaction |
DE69028325T2 (de) * | 1989-01-19 | 1997-04-17 | Behringwerke Ag | Nukleinsäure-Amplifikation unter Verwendung eines Einzelprimers |
US5508178A (en) * | 1989-01-19 | 1996-04-16 | Rose; Samuel | Nucleic acid amplification using single primer |
CA2012982A1 (en) * | 1989-03-27 | 1990-09-27 | Frank W. Hobbs | Process for rapid nucleic acid detection by incorporating a reporter moiety into amplified target nucleic acid |
-
1990
- 1990-07-19 US US07/555,323 patent/US5595891A/en not_active Expired - Fee Related
-
1991
- 1991-07-18 CA CA002047342A patent/CA2047342A1/en not_active Abandoned
- 1991-07-18 EP EP91306550A patent/EP0469755B1/de not_active Expired - Lifetime
- 1991-07-18 JP JP3269946A patent/JPH0584079A/ja active Pending
- 1991-07-18 ES ES91306550T patent/ES2091876T3/es not_active Expired - Lifetime
- 1991-07-18 AT AT91306550T patent/ATE143697T1/de active
- 1991-07-18 DE DE69122457T patent/DE69122457T2/de not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
ES2091876T3 (es) | 1996-11-16 |
ATE143697T1 (de) | 1996-10-15 |
EP0469755B1 (de) | 1996-10-02 |
JPH0584079A (ja) | 1993-04-06 |
EP0469755A1 (de) | 1992-02-05 |
US5595891A (en) | 1997-01-21 |
CA2047342A1 (en) | 1992-01-20 |
DE69122457T2 (de) | 1997-03-20 |
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ATE161586T1 (de) | Verfahren zur herstellung eines polynukleotids zum gebrauch in ''single-primer'' amplifizierung und phosphorothioat-enthaltende oligonukleotide als primer in nukleinsäureamplifizierung | |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8327 | Change in the person/name/address of the patent owner |
Owner name: DADE BEHRING MARBURG GMBH, 35041 MARBURG, DE |
|
8339 | Ceased/non-payment of the annual fee |