DE69122457T2 - Verfahren zur Herstellung eines Polynukleotides zur Verwendung bei Einzelprimeramplifikation - Google Patents

Verfahren zur Herstellung eines Polynukleotides zur Verwendung bei Einzelprimeramplifikation

Info

Publication number
DE69122457T2
DE69122457T2 DE69122457T DE69122457T DE69122457T2 DE 69122457 T2 DE69122457 T2 DE 69122457T2 DE 69122457 T DE69122457 T DE 69122457T DE 69122457 T DE69122457 T DE 69122457T DE 69122457 T2 DE69122457 T2 DE 69122457T2
Authority
DE
Germany
Prior art keywords
extended
primer
polynucleotide
extender probe
duplex
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69122457T
Other languages
English (en)
Other versions
DE69122457D1 (de
Inventor
Samuel Rose
Linda M Western
Martin Becker
Edwin F Ullman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Behringwerke AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke AG filed Critical Behringwerke AG
Publication of DE69122457D1 publication Critical patent/DE69122457D1/de
Application granted granted Critical
Publication of DE69122457T2 publication Critical patent/DE69122457T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
DE69122457T 1990-07-19 1991-07-18 Verfahren zur Herstellung eines Polynukleotides zur Verwendung bei Einzelprimeramplifikation Expired - Fee Related DE69122457T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/555,323 US5595891A (en) 1990-07-19 1990-07-19 Method for producing a polynucleotide for use in single primer amplification

Publications (2)

Publication Number Publication Date
DE69122457D1 DE69122457D1 (de) 1996-11-07
DE69122457T2 true DE69122457T2 (de) 1997-03-20

Family

ID=24216831

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69122457T Expired - Fee Related DE69122457T2 (de) 1990-07-19 1991-07-18 Verfahren zur Herstellung eines Polynukleotides zur Verwendung bei Einzelprimeramplifikation

Country Status (7)

Country Link
US (1) US5595891A (de)
EP (1) EP0469755B1 (de)
JP (1) JPH0584079A (de)
AT (1) ATE143697T1 (de)
CA (1) CA2047342A1 (de)
DE (1) DE69122457T2 (de)
ES (1) ES2091876T3 (de)

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CA2080305A1 (en) 1991-10-11 1993-04-12 Linda M. Western Method for producing a polynucleotide for use in single primer amplification and phosphorothioate-containing oligonucleotides as primers in nucleic acid amplification
ZA929319B (en) * 1991-12-11 1993-05-24 Igen Inc Method for exponential amplification of nucleic acid by a single unpaired primer.
AU3728093A (en) * 1992-02-19 1993-09-13 Public Health Research Institute Of The City Of New York, Inc., The Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
GB9210176D0 (en) * 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
GB9210177D0 (en) * 1992-05-12 1992-06-24 Cemu Bioteknik Ab Loop structures
DE69310179T2 (de) * 1992-07-31 1997-07-31 Behringwerke Ag Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
US6294323B1 (en) * 1993-04-14 2001-09-25 Behringwerke Ag Self initiating single primer amplification of nucleic acids
US5470723A (en) * 1993-05-05 1995-11-28 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
US5681697A (en) * 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
WO1997004131A1 (en) * 1995-07-21 1997-02-06 Forsyth Dental Infirmary For Children Single primer amplification of polynucleotide hairpins
DE69636838T2 (de) * 1995-11-25 2007-10-18 Roche Diagnostics Gmbh Verfahren zur herstellung vielfältiger doppelsträngiger nukleinsäuren
DE69633941T2 (de) * 1995-12-22 2005-11-24 Dade Behring Marburg Gmbh Nachweis von unterschieden in nukleinsäuren
US6326143B1 (en) 1996-11-22 2001-12-04 Roche Diagnostics Gmbh Method for generating multiple double stranded nucleic acids
US8445664B2 (en) * 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
EP2287338B1 (de) 1998-11-09 2012-09-05 Eiken Kagaku Kabushiki Kaisha Prozess zur Synthetisierung von Nukleinsäure
US6232104B1 (en) 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
CN102586228A (zh) 1999-09-13 2012-07-18 纽亘技术公司 用于多核苷酸序列线性等温扩增的方法及组合物
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
ATE317023T1 (de) 1999-11-08 2006-02-15 Eiken Chemical Methode zum nachweis von variationen oder polymorphismen
JP4721603B2 (ja) * 1999-11-08 2011-07-13 栄研化学株式会社 変異および／または多型の検出方法
ES2464132T3 (es) * 2000-04-07 2014-05-30 Eiken Kagaku Kabushiki Kaisha Procedimiento de amplificación de ácido nucleico utilizando como molde ácido nucleico bicatenario
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
DE60141087D1 (de) 2000-06-26 2010-03-04 Nugen Technologies Inc Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren
AU2002230734A1 (en) 2000-12-11 2002-06-24 Alexion Pharmaceuticals, Inc. Nested oligonucleotides containing hairpin for nucleic acid amplification
US6858413B2 (en) 2000-12-13 2005-02-22 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
BR0205268A (pt) 2001-03-09 2004-11-30 Nugen Technologies Inc Processos e composições para a mplificação de sequências de rna
EP1390537B1 (de) * 2001-03-09 2013-11-13 Nugen Technologies, Inc. Verfahren und zusammensetzungen zur amplifikation von rna-sequenzen
US7414111B2 (en) * 2001-09-19 2008-08-19 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
WO2003025202A2 (en) * 2001-09-19 2003-03-27 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
US7045319B2 (en) * 2001-10-30 2006-05-16 Ribomed Biotechnologies, Inc. Molecular detection systems utilizing reiterative oligonucleotide synthesis
US20040054162A1 (en) * 2001-10-30 2004-03-18 Hanna Michelle M. Molecular detection systems utilizing reiterative oligonucleotide synthesis
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
US7510829B2 (en) * 2001-11-19 2009-03-31 Affymetrix, Inc. Multiplex PCR
GB0218215D0 (en) * 2002-08-06 2002-09-11 Tepnel Medical Ltd Amplification of nucleic acids
CA2521084A1 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
AU2004286265A1 (en) * 2003-10-29 2005-05-12 Ribomed Biotechnologies, Inc. Compositions, methods and detection technologies for reiterative oligonucleotide synthesis
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
US8137936B2 (en) * 2005-11-29 2012-03-20 Macevicz Stephen C Selected amplification of polynucleotides
US20090203531A1 (en) * 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
WO2009117698A2 (en) 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
WO2009140666A2 (en) 2008-05-15 2009-11-19 Ribomed Biotechnologies, Inc. METHODS AND REAGENTS FOR DETECTING CpG METHYLATION WITH A METHYL CpG BINDING PROTEIN (MBP)
US8211644B2 (en) * 2008-07-13 2012-07-03 Ribomed Biotechnologies, Inc. Molecular beacon-based methods for detection of targets using abscription
KR20120007002A (ko) 2009-03-15 2012-01-19 리보메드 바이오테크놀로지스, 인코퍼레이티드 압스크립션 기반 분자 검출
CA2686250C (en) 2009-11-12 2016-10-11 Fountainhead, Llc Floating treatment streambed
JP2015536672A (ja) 2012-11-28 2015-12-24 エービーウィズ バイオ，インク． 単一プライマー増幅における使用のための遺伝子特異的なテンプレートの調製
US10694693B2 (en) 2015-12-18 2020-06-30 Monsanto Technology Llc Methods for producing corn plants with northern leaf blight resistance and compositions thereof

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US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4868104A (en) * 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US5066584A (en) * 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5508178A (en) * 1989-01-19 1996-04-16 Rose; Samuel Nucleic acid amplification using single primer
CA2008096A1 (en) * 1989-01-19 1990-07-19 Samuel Rose Nucleic acid amplification using single primer
CA2012982A1 (en) * 1989-03-27 1990-09-27 Frank W. Hobbs Process for rapid nucleic acid detection by incorporating a reporter moiety into amplified target nucleic acid

Also Published As

Publication number Publication date
ES2091876T3 (es) 1996-11-16
DE69122457D1 (de) 1996-11-07
EP0469755A1 (de) 1992-02-05
JPH0584079A (ja) 1993-04-06
ATE143697T1 (de) 1996-10-15
CA2047342A1 (en) 1992-01-20
US5595891A (en) 1997-01-21
EP0469755B1 (de) 1996-10-02

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: DADE BEHRING MARBURG GMBH, 35041 MARBURG, DE

8339 Ceased/non-payment of the annual fee