DE69125380T2 - Durch reduktion orthogonal entfernbare hydroxylschutzgruppen und ihre verwendung in der chemischen synthese von oligonukleotiden - Google Patents

Durch reduktion orthogonal entfernbare hydroxylschutzgruppen und ihre verwendung in der chemischen synthese von oligonukleotiden

Info

Publication number
DE69125380T2
DE69125380T2 DE69125380T DE69125380T DE69125380T2 DE 69125380 T2 DE69125380 T2 DE 69125380T2 DE 69125380 T DE69125380 T DE 69125380T DE 69125380 T DE69125380 T DE 69125380T DE 69125380 T2 DE69125380 T2 DE 69125380T2
Authority
DE
Germany
Prior art keywords
chemical synthesis
hydroxyl
oligonucleotides
protective groups
protecting groups
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69125380T
Other languages
English (en)
Other versions
DE69125380D1 (de
Inventor
Michael Urdea
Thomas Horn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics Inc
Original Assignee
Chiron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Corp filed Critical Chiron Corp
Application granted granted Critical
Publication of DE69125380D1 publication Critical patent/DE69125380D1/de
Publication of DE69125380T2 publication Critical patent/DE69125380T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups
DE69125380T 1990-07-27 1991-07-24 Durch reduktion orthogonal entfernbare hydroxylschutzgruppen und ihre verwendung in der chemischen synthese von oligonukleotiden Expired - Lifetime DE69125380T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/558,881 US5430138A (en) 1990-07-27 1990-07-27 Hydroxyl-protecting groups attached to cytidine nucleotide compounds which are orthogonally removable
PCT/US1991/005227 WO1992002533A1 (en) 1990-07-27 1991-07-24 Hydroxyl-protecting groups orthogonally removable by reduction and their use in the chemical synthesis of oligonucleotides

Publications (2)

Publication Number Publication Date
DE69125380D1 DE69125380D1 (de) 1997-04-30
DE69125380T2 true DE69125380T2 (de) 1997-08-28

Family

ID=24231370

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69125380T Expired - Lifetime DE69125380T2 (de) 1990-07-27 1991-07-24 Durch reduktion orthogonal entfernbare hydroxylschutzgruppen und ihre verwendung in der chemischen synthese von oligonukleotiden

Country Status (7)

Country Link
US (2) US5430138A (de)
EP (1) EP0543906B1 (de)
JP (1) JP3081882B2 (de)
AT (1) ATE150760T1 (de)
CA (1) CA2088255A1 (de)
DE (1) DE69125380T2 (de)
WO (1) WO1992002533A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10133779A1 (de) * 2001-07-16 2003-02-06 Chemogenix Gmbh Multimerpolynukleotidsynthese

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5430138A (en) * 1990-07-27 1995-07-04 Chiron Corporation Hydroxyl-protecting groups attached to cytidine nucleotide compounds which are orthogonally removable
TW323284B (de) * 1992-03-23 1997-12-21 Novartis Ag
US5770725A (en) * 1992-05-25 1998-06-23 Gosselin; Gilles Phosphotriester type biologically active compounds
US6020482A (en) * 1992-05-25 2000-02-01 Gosselin; Gilles Phosphotriester type biologically active compounds
FR2692265B1 (fr) 1992-05-25 1996-11-08 Centre Nat Rech Scient Composes biologiquement actifs de type phosphotriesters.
US5849905A (en) * 1994-11-23 1998-12-15 Centre National De La Recherche Scientifique Biologically active phosphotriester-type nucleosides and methods for preparing same
US5955591A (en) * 1993-05-12 1999-09-21 Imbach; Jean-Louis Phosphotriester oligonucleotides, amidites and method of preparation
US5597909A (en) * 1994-08-25 1997-01-28 Chiron Corporation Polynucleotide reagents containing modified deoxyribose moieties, and associated methods of synthesis and use
US5639875A (en) * 1995-02-01 1997-06-17 Hybridon, Inc. Methods for H-phosphonate syntheis of oligonucleotides using triphosgene
WO1997014812A2 (en) * 1995-10-16 1997-04-24 Chiron Corporation Method of screening for factors that modulate gene expression
DE69732101T2 (de) * 1996-04-17 2005-12-22 Köster, Hubert, Dr., Cambridge Kombinatorische schutzgruppestrategie für multifunktionelle molekule
WO1998035707A1 (en) 1997-02-18 1998-08-20 Thomas Jefferson University Compositions that bind to pancreatic cancer cells and methods of using the same
US6120995A (en) * 1997-08-07 2000-09-19 Thomas Jefferson University Compositions that specifically bind to colorectal cancer cells and methods of using the same
US7135333B1 (en) 1997-08-07 2006-11-14 Thomas Jefferson University Compositions that specifically bind to colorectal cancer cells and methods of using the same
WO2000018778A1 (en) * 1998-09-29 2000-04-06 Phylos, Inc. Synthesis of codon randomized nucleic acids
US6965041B1 (en) 1999-03-24 2005-11-15 The United States Of America As Represented By The Department Of Health And Human Services N-acylphosphoramidites and their use in oligonucleotide synthesis
DE60142228D1 (de) 2000-03-27 2010-07-08 Univ Jefferson Zusammensetzungen und methoden zur identifizierung und zum targeting von krebszellen aus dem verdauungskanal
AU2001292728A1 (en) * 2000-09-18 2002-03-26 Thomas Jefferson University Compositions and methods for identifying and targeting stomach and esophageal cancer cells
US6932943B1 (en) 2001-01-26 2005-08-23 Third Wave Technologies Nucleic acid synthesizers
US7435390B2 (en) * 2001-01-26 2008-10-14 Third Wave Technologies, Inc. Nucleic acid synthesizers
WO2003048179A2 (en) * 2001-12-03 2003-06-12 The Government Of The United States Of America, Represented By The Secretary Of The Department Of Health And Human Services Thermolabile hydroxyl protecting groups and methods of use
AUPR975201A0 (en) * 2001-12-24 2002-01-24 Unisearch Limited Enzymatic redox labelling of nucleic acids
DE10247790A1 (de) * 2002-10-14 2004-04-22 Chemogenix Gmbh Verfahren zur Herstellung von Oligonukleotidkonjugaten
JP2006516099A (ja) * 2002-12-23 2006-06-22 ダイナバックス テクノロジーズ コーポレイション 分枝状の免疫調節化合物及び該化合物の使用方法
NZ542687A (en) 2003-04-13 2010-02-26 Enzon Pharmaceuticals Inc Polymeric oligonucleotide prodrugs
WO2006065751A2 (en) * 2004-12-13 2006-06-22 Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Cpg oligonucleotide prodrugs, compositions thereof and associated therapeutic methods
EP1899361B1 (de) 2005-05-26 2013-12-11 Luminex Corporation Reagens für die verbesserte synthese von isoguanosinhaltigen oligonukleotiden
KR100834812B1 (ko) 2006-07-04 2008-06-05 삼성전자주식회사 이동통신 시스템에서 세션 이동 횟수를 줄이는 핸드오프를위한 장치 및 방법
EP2056709A4 (de) 2006-08-11 2013-05-01 Univ New Jersey Med Zweifachsensibilisator mit lumineszenzverbindungen, konjugaten und verwendung
US9221759B2 (en) 2010-01-13 2015-12-29 Rutgers, The State University Of New Jersey Fluorophore chelated lanthanide luminescent probes with improved quantum efficiency
WO2020236618A1 (en) * 2019-05-17 2020-11-26 Ionis Pharmaceuticals, Inc. Synthesis of oligomeric compounds comprising phosphorothioate diester and phosphate diester linkages

Family Cites Families (9)

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Publication number Priority date Publication date Assignee Title
US5118802A (en) * 1983-12-20 1992-06-02 California Institute Of Technology DNA-reporter conjugates linked via the 2' or 5'-primary amino group of the 5'-terminal nucleoside
US4843122A (en) * 1984-01-30 1989-06-27 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
EP0305643B1 (de) * 1984-04-18 1994-01-05 Whitby Research Incorporated N-6-thienyl-substituierte Adenosinderivate als herzgefässerweiternde Mittel
US5367066A (en) * 1984-10-16 1994-11-22 Chiron Corporation Oligonucleotides with selectably cleavable and/or abasic sites
US4910300A (en) * 1985-12-11 1990-03-20 Chiron Corporation Method for making nucleic acid probes
US5093232A (en) * 1985-12-11 1992-03-03 Chiron Corporation Nucleic acid probes
US4837311A (en) * 1987-06-22 1989-06-06 Hoffman-La Roche Inc. Anti-retroviral compounds
CA1339351C (en) * 1987-10-15 1997-08-26 Michael S. Urdea Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5430138A (en) * 1990-07-27 1995-07-04 Chiron Corporation Hydroxyl-protecting groups attached to cytidine nucleotide compounds which are orthogonally removable

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10133779A1 (de) * 2001-07-16 2003-02-06 Chemogenix Gmbh Multimerpolynukleotidsynthese

Also Published As

Publication number Publication date
JPH05509317A (ja) 1993-12-22
CA2088255A1 (en) 1992-01-28
WO1992002533A1 (en) 1992-02-20
JP3081882B2 (ja) 2000-08-28
ATE150760T1 (de) 1997-04-15
US5430138A (en) 1995-07-04
US5703218A (en) 1997-12-30
EP0543906A4 (en) 1993-07-14
EP0543906B1 (de) 1997-03-26
EP0543906A1 (de) 1993-06-02
DE69125380D1 (de) 1997-04-30

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: CHIRON DIAGNOSTICS CORP. (N.D.GES.D. STAATES DELAW

8327 Change in the person/name/address of the patent owner

Owner name: SIEMENS HEALTHCARE DIAGNOSTICS INC. (N.D.GES.D, US

8328 Change in the person/name/address of the agent

Representative=s name: MAIER, D., DIPL.-ING. UNIV., PAT.-ASS., 81739 MUEN