DE69225332D1 - Präparation von reinkulturen von humanen post-mitotischen neuronen - Google Patents

Präparation von reinkulturen von humanen post-mitotischen neuronen

Info

Publication number
DE69225332D1
DE69225332D1 DE69225332T DE69225332T DE69225332D1 DE 69225332 D1 DE69225332 D1 DE 69225332D1 DE 69225332 T DE69225332 T DE 69225332T DE 69225332 T DE69225332 T DE 69225332T DE 69225332 D1 DE69225332 D1 DE 69225332D1
Authority
DE
Germany
Prior art keywords
cells
pure
preparation
mitotic
cultures
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69225332T
Other languages
English (en)
Other versions
DE69225332T2 (de
Inventor
Virginia Lee
Samuel Pleasure
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Pennsylvania Penn
Original Assignee
University of Pennsylvania Penn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Pennsylvania Penn filed Critical University of Pennsylvania Penn
Application granted granted Critical
Publication of DE69225332D1 publication Critical patent/DE69225332D1/de
Publication of DE69225332T2 publication Critical patent/DE69225332T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C41/00—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor
    • B29C41/02—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor for making articles of definite length, i.e. discrete articles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C41/00—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor
    • B29C41/02—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor for making articles of definite length, i.e. discrete articles
    • B29C41/12—Spreading-out the material on a substrate, e.g. on the surface of a liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C41/00—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor
    • B29C41/34—Component parts, details or accessories; Auxiliary operations
    • B29C41/36—Feeding the material on to the mould, core or other substrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29L—INDEXING SCHEME ASSOCIATED WITH SUBCLASS B29C, RELATING TO PARTICULAR ARTICLES
    • B29L2023/00—Tubular articles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/30—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from cancer cells, e.g. reversion of tumour cells
DE69225332T 1991-10-21 1992-08-05 Präparation von reinkulturen von humanen post-mitotischen neuronen Expired - Fee Related DE69225332T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/780,715 US5175103A (en) 1991-10-21 1991-10-21 Preparation of pure cultures of post-mitotic human neurons
PCT/US1992/006540 WO1993008266A1 (en) 1991-10-21 1992-08-05 Preparation of pure cultures of post-mitotic human neurons

Publications (2)

Publication Number Publication Date
DE69225332D1 true DE69225332D1 (de) 1998-06-04
DE69225332T2 DE69225332T2 (de) 1998-08-27

Family

ID=25120453

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69225332T Expired - Fee Related DE69225332T2 (de) 1991-10-21 1992-08-05 Präparation von reinkulturen von humanen post-mitotischen neuronen

Country Status (9)

Country Link
US (2) US5175103A (de)
EP (1) EP0620847B1 (de)
JP (1) JP3169611B2 (de)
AT (1) ATE165617T1 (de)
CA (1) CA2120730A1 (de)
DE (1) DE69225332T2 (de)
DK (1) DK0620847T3 (de)
ES (1) ES2117672T3 (de)
WO (1) WO1993008266A1 (de)

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US7049102B1 (en) 1989-09-22 2006-05-23 Board Of Trustees Of Leland Stanford University Multi-gene expression profile
US5545522A (en) 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US7361505B1 (en) 1991-07-08 2008-04-22 Neurospheres Holdings Ltd. Multipotent neural stem cell compositions
US6399369B1 (en) 1991-07-08 2002-06-04 Neurospheres Holdings Ltd. Multipotent neural stem cell cDNA libraries
US7166277B1 (en) 1991-07-08 2007-01-23 Neurospheres Holdings Ltd. Remyelination of neurons using multipotent neural stem cell progeny
US5750376A (en) * 1991-07-08 1998-05-12 Neurospheres Holdings Ltd. In vitro growth and proliferation of genetically modified multipotent neural stem cells and their progeny
US5851832A (en) * 1991-07-08 1998-12-22 Neurospheres, Ltd. In vitro growth and proliferation of multipotent neural stem cells and their progeny
US5980885A (en) * 1991-07-08 1999-11-09 Neurospheres Holdings Ltd. Growth factor-induced proliferation of neural precursor cells in vivo
US6071889A (en) * 1991-07-08 2000-06-06 Neurospheres Holdings Ltd. In vivo genetic modification of growth factor-responsive neural precursor cells
US6497872B1 (en) * 1991-07-08 2002-12-24 Neurospheres Holdings Ltd. Neural transplantation using proliferated multipotent neural stem cells and their progeny
US6294346B1 (en) 1991-07-08 2001-09-25 Neurospheres Holdings, Ltd. Use of multipotent neural stem cells and their progeny for the screening of drugs and other biological agents
US5175103A (en) * 1991-10-21 1992-12-29 Trustees Of University Of Pennsylvania Preparation of pure cultures of post-mitotic human neurons
US6214334B1 (en) * 1991-10-21 2001-04-10 Trustees Of The University Of Pennsylvania Compositions and methods for producing and using homogenous neuronal cell transplants to treat neurodegenerative disorders and brain and spinal cord injuries
US5792900A (en) * 1991-10-21 1998-08-11 The Trustees Of The University Of Pennsylvania Compositions and methods for producing and using homogenous neuronal cell transplants
CA2176216A1 (en) * 1993-11-09 1995-05-18 Virginia M.-Y. Lee Compositions and methods for producing and using homogeneous neuronal cell transplants
US7011827B2 (en) * 1993-11-09 2006-03-14 Trustees Of The University Of Pennsylvania Compositions and methods for producing and using homogenous neuronal cell transplants
US5449609A (en) * 1994-01-31 1995-09-12 Children's Hospital Of Philadelphia Methods for screening for neurotoxicity using a clonal human teratocarcinoma cell line
US20060029575A1 (en) * 1994-11-09 2006-02-09 Lee Virginia M Compositions and methods for producing and using homogenous neuronal cell transplants
CN1170435A (zh) * 1994-11-14 1998-01-14 纽罗斯菲里斯控股有限公司 神经干细胞增殖的调节
US5753505A (en) * 1995-07-06 1998-05-19 Emory University Neuronal progenitor cells and uses thereof
US7544511B2 (en) * 1996-09-25 2009-06-09 Neuralstem Biopharmaceuticals Ltd. Stable neural stem cell line methods
US6162428A (en) * 1997-02-12 2000-12-19 Layton Bioscience, Inc. hNT-neuron human neuronal cells to replace ganglion cells
WO1998049271A1 (en) * 1997-04-25 1998-11-05 American Home Products Corporation Human neuronal cell line
US6254865B1 (en) 1997-06-17 2001-07-03 University Technology Corporation Method of treating huntington's disease using HNT neurons
CA2294737A1 (en) * 1997-07-04 1999-01-14 University Of Utah Research Foundation Neuron-restricted precursor cells
US6734015B1 (en) * 1997-07-04 2004-05-11 University Of Utah Research Foundation Isolation of lineage-restricted neuronal precursors
US6235527B1 (en) * 1997-11-29 2001-05-22 University Of Utah Research Foundation Lineage restricted glial precursors from the central nervous system
US5968829A (en) * 1997-09-05 1999-10-19 Cytotherapeutics, Inc. Human CNS neural stem cells
WO1999013726A1 (en) * 1997-09-19 1999-03-25 University Of South Florida Neuronal cells and an immunosuppressant and anti-inflammatory factor
WO2000006700A1 (en) * 1998-07-29 2000-02-10 Layton Bioscience, Inc. Production and use of dopaminergic cells to treat dopaminergic deficiencies
US20030211085A1 (en) * 2000-03-16 2003-11-13 Sanberg Paul R. Cell therapy for chronic stroke
EP1949904A3 (de) 1999-04-27 2008-08-06 Layton Bioscience, Inc. Zelltherapie für chronische Apoplexie
DE19928210B4 (de) * 1999-06-19 2005-08-18 Neuroprogen Gmbh Leipzig Neuronales Zellmaterial und Verfahren zu dessen Herstellung
US6749850B1 (en) 1999-08-18 2004-06-15 The General Hospital Corporation Methods, compositions and kits for promoting recovery from damage to the central nervous system
US7785882B2 (en) * 2000-01-18 2010-08-31 Cornell Research Foundation, Inc. Neuronal progenitor cells from hippocampal tissue and a method for isolating and purifying them
AU2001253425A1 (en) 2000-04-13 2001-10-30 Board Of Regents, The University Of Texas System Treatment of disorders by implanting stem cells and/or progeny thereof into gastrointestinal organs
US7037493B2 (en) 2000-05-01 2006-05-02 Cornell Research Foundation, Inc. Method of inducing neuronal production in the brain and spinal cord
US6897061B1 (en) 2000-06-16 2005-05-24 Spinal Cord Society Transdifferentiation of glial cells
JP4031901B2 (ja) * 2000-07-19 2008-01-09 株式会社東芝 固体撮像装置
WO2002028850A1 (fr) * 2000-10-05 2002-04-11 Takeda Chemical Industries, Ltd. Promoteurs de proliferation et de differenciation de cellules embryonnaires et/ou de precurseurs neuronaux
AU2002251681A1 (en) * 2000-12-05 2002-08-28 Layton Bioscience Inc. Production and use of dopaminergic cells to treat dopaminergic deficiencies
WO2002099087A2 (de) * 2001-06-05 2002-12-12 Thomas Rohrmeier Zellpopulationen zur auffindung neuronaler targets und potentieller wirkstoffe
WO2003070189A2 (en) * 2002-02-15 2003-08-28 Cornell Research Foundation, Inc. Enhancing neurotrophin-induced neurogenesis by endogenous neural progenitor cells by concurrent overexpression of brain derived neurotrophic factor and an inhibitor of a pro-gliogenic bone morphogenetic protein
GB0217981D0 (en) * 2002-08-02 2002-09-11 Univ Durham Method for preparation, purification and differentiation of neurospheres from mammalian stem cells
US8293488B2 (en) 2002-12-09 2012-10-23 Neuralstem, Inc. Method for screening neurogenic agents
AU2003293409A1 (en) 2002-12-09 2004-06-30 Karl K. Johe Method for discovering neurogenic agents
DE10259617A1 (de) * 2002-12-18 2004-07-08 Cardion Ag Verfahren zur Inhibierung des Proliferationspotentials von Zellpopulationen und Geweben
WO2004098621A1 (en) * 2003-04-17 2004-11-18 U.S. Department Of Veterans Affairs Isolated/cloned human nt2 cell lines expressing serotonin and gaba
FR2859219B1 (fr) * 2003-09-02 2005-10-14 Alain Privat Procede de production de neurones a partir de cellules d'une lignee cellulaire
GB2414995B (en) 2004-06-09 2006-11-15 Univ Edinburgh Neural stem cells
US7663614B2 (en) * 2004-10-05 2010-02-16 Jeff Maynard Method for storing and comparing computer generated lines
KR101429284B1 (ko) 2004-11-17 2014-08-11 뉴럴스템, 인크. 신경퇴행성 증상의 치료를 위한 인간 신경 세포의 이식
US7736641B2 (en) * 2007-02-21 2010-06-15 Prince Henry's Institute Of Medical Research Role for SRY in Parkinson's disease
US20090136461A1 (en) * 2007-11-28 2009-05-28 Moon Suk Kim Neuronal differentiation method of adult stem cells using small molecules
CN102325878A (zh) 2008-12-23 2012-01-18 斯特姆塞尔思加利福尼亚有限公司 少突胶质前体细胞的靶向细胞群及制备与使用方法
US9101570B1 (en) 2009-02-13 2015-08-11 Endocellutions, Inc. Adult and neonatal stem cell therapy to treat diabetes through the repair of the gastrointestinal tract
AU2011282642B2 (en) 2010-07-28 2015-06-11 Shinn-Zong Lin Methods for treating and/or reversing neurodegenerative diseases and/or disorders
KR20190060016A (ko) 2014-10-20 2019-05-31 뉴럴스템, 인크. 성장 인자를 코딩하는 외인성 폴리뉴클레오티드를 포함하는 안정한 신경 줄기세포 및 그의 사용 방법

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US4829000A (en) * 1985-08-30 1989-05-09 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Reconstituted basement membrane complex with biological activity
US5032407A (en) * 1987-01-16 1991-07-16 Ohio University Edison Animal Biotechnology Center Gene transfer using transformed, neodetermined, embryonic cells
US5270191A (en) * 1988-04-12 1993-12-14 Massachusetts Institute Of Technology Method for manipulation of the cell types of eukaryotes
FR2645866B1 (fr) * 1989-04-17 1991-07-05 Centre Nat Rech Scient Nouvelles lipopolyamines, leur preparation et leur emploi
US5180820A (en) * 1989-08-30 1993-01-19 Barde Yves Alain Brain-derived neurotrophic factor
US5175103A (en) * 1991-10-21 1992-12-29 Trustees Of University Of Pennsylvania Preparation of pure cultures of post-mitotic human neurons

Also Published As

Publication number Publication date
JPH07502645A (ja) 1995-03-23
WO1993008266A1 (en) 1993-04-29
EP0620847A1 (de) 1994-10-26
EP0620847B1 (de) 1998-04-29
US5175103A (en) 1992-12-29
CA2120730A1 (en) 1993-04-29
EP0620847A4 (de) 1995-03-29
DE69225332T2 (de) 1998-08-27
ATE165617T1 (de) 1998-05-15
ES2117672T3 (es) 1998-08-16
DK0620847T3 (da) 1998-10-07
US5654189A (en) 1997-08-05
JP3169611B2 (ja) 2001-05-28

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Legal Events

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8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: VONNEMANN, KLOIBER & KOLLEGEN, 80796 MUENCHEN

8339 Ceased/non-payment of the annual fee