DE69225332T2 - Präparation von reinkulturen von humanen post-mitotischen neuronen - Google Patents
Präparation von reinkulturen von humanen post-mitotischen neuronenInfo
- Publication number
- DE69225332T2 DE69225332T2 DE69225332T DE69225332T DE69225332T2 DE 69225332 T2 DE69225332 T2 DE 69225332T2 DE 69225332 T DE69225332 T DE 69225332T DE 69225332 T DE69225332 T DE 69225332T DE 69225332 T2 DE69225332 T2 DE 69225332T2
- Authority
- DE
- Germany
- Prior art keywords
- cells
- pure
- preparation
- mitotic
- cultures
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C41/00—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor
- B29C41/02—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor for making articles of definite length, i.e. discrete articles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C41/00—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor
- B29C41/02—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor for making articles of definite length, i.e. discrete articles
- B29C41/12—Spreading-out the material on a substrate, e.g. on the surface of a liquid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C41/00—Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor
- B29C41/34—Component parts, details or accessories; Auxiliary operations
- B29C41/36—Feeding the material on to the mould, core or other substrate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0619—Neurons
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29L—INDEXING SCHEME ASSOCIATED WITH SUBCLASS B29C, RELATING TO PARTICULAR ARTICLES
- B29L2023/00—Tubular articles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/30—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from cancer cells, e.g. reversion of tumour cells
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/780,715 US5175103A (en) | 1991-10-21 | 1991-10-21 | Preparation of pure cultures of post-mitotic human neurons |
PCT/US1992/006540 WO1993008266A1 (en) | 1991-10-21 | 1992-08-05 | Preparation of pure cultures of post-mitotic human neurons |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69225332D1 DE69225332D1 (de) | 1998-06-04 |
DE69225332T2 true DE69225332T2 (de) | 1998-08-27 |
Family
ID=25120453
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69225332T Expired - Fee Related DE69225332T2 (de) | 1991-10-21 | 1992-08-05 | Präparation von reinkulturen von humanen post-mitotischen neuronen |
Country Status (9)
Country | Link |
---|---|
US (2) | US5175103A (de) |
EP (1) | EP0620847B1 (de) |
JP (1) | JP3169611B2 (de) |
AT (1) | ATE165617T1 (de) |
CA (1) | CA2120730A1 (de) |
DE (1) | DE69225332T2 (de) |
DK (1) | DK0620847T3 (de) |
ES (1) | ES2117672T3 (de) |
WO (1) | WO1993008266A1 (de) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10259617A1 (de) * | 2002-12-18 | 2004-07-08 | Cardion Ag | Verfahren zur Inhibierung des Proliferationspotentials von Zellpopulationen und Geweben |
Families Citing this family (57)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7049102B1 (en) | 1989-09-22 | 2006-05-23 | Board Of Trustees Of Leland Stanford University | Multi-gene expression profile |
US5545522A (en) | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
US7361505B1 (en) | 1991-07-08 | 2008-04-22 | Neurospheres Holdings Ltd. | Multipotent neural stem cell compositions |
US7166277B1 (en) | 1991-07-08 | 2007-01-23 | Neurospheres Holdings Ltd. | Remyelination of neurons using multipotent neural stem cell progeny |
US5750376A (en) * | 1991-07-08 | 1998-05-12 | Neurospheres Holdings Ltd. | In vitro growth and proliferation of genetically modified multipotent neural stem cells and their progeny |
US6071889A (en) * | 1991-07-08 | 2000-06-06 | Neurospheres Holdings Ltd. | In vivo genetic modification of growth factor-responsive neural precursor cells |
US6294346B1 (en) | 1991-07-08 | 2001-09-25 | Neurospheres Holdings, Ltd. | Use of multipotent neural stem cells and their progeny for the screening of drugs and other biological agents |
US5980885A (en) * | 1991-07-08 | 1999-11-09 | Neurospheres Holdings Ltd. | Growth factor-induced proliferation of neural precursor cells in vivo |
US6497872B1 (en) | 1991-07-08 | 2002-12-24 | Neurospheres Holdings Ltd. | Neural transplantation using proliferated multipotent neural stem cells and their progeny |
US5851832A (en) * | 1991-07-08 | 1998-12-22 | Neurospheres, Ltd. | In vitro growth and proliferation of multipotent neural stem cells and their progeny |
US6399369B1 (en) | 1991-07-08 | 2002-06-04 | Neurospheres Holdings Ltd. | Multipotent neural stem cell cDNA libraries |
US5175103A (en) * | 1991-10-21 | 1992-12-29 | Trustees Of University Of Pennsylvania | Preparation of pure cultures of post-mitotic human neurons |
US6214334B1 (en) * | 1991-10-21 | 2001-04-10 | Trustees Of The University Of Pennsylvania | Compositions and methods for producing and using homogenous neuronal cell transplants to treat neurodegenerative disorders and brain and spinal cord injuries |
US5792900A (en) * | 1991-10-21 | 1998-08-11 | The Trustees Of The University Of Pennsylvania | Compositions and methods for producing and using homogenous neuronal cell transplants |
WO1995012982A1 (en) * | 1993-11-09 | 1995-05-18 | The Trustees Of The University Of Pennsylvania | Compositions and methods for producing and using homogeneous neuronal cell transplants |
US7011827B2 (en) * | 1993-11-09 | 2006-03-14 | Trustees Of The University Of Pennsylvania | Compositions and methods for producing and using homogenous neuronal cell transplants |
US5449609A (en) * | 1994-01-31 | 1995-09-12 | Children's Hospital Of Philadelphia | Methods for screening for neurotoxicity using a clonal human teratocarcinoma cell line |
US20060029575A1 (en) * | 1994-11-09 | 2006-02-09 | Lee Virginia M | Compositions and methods for producing and using homogenous neuronal cell transplants |
WO1996015226A1 (en) * | 1994-11-14 | 1996-05-23 | Neurospheres Holdings Ltd. | Regulation of neural stem cell proliferation |
US5753505A (en) | 1995-07-06 | 1998-05-19 | Emory University | Neuronal progenitor cells and uses thereof |
US7544511B2 (en) * | 1996-09-25 | 2009-06-09 | Neuralstem Biopharmaceuticals Ltd. | Stable neural stem cell line methods |
US6162428A (en) * | 1997-02-12 | 2000-12-19 | Layton Bioscience, Inc. | hNT-neuron human neuronal cells to replace ganglion cells |
AU6897498A (en) * | 1997-04-25 | 1998-11-24 | American Home Products Corporation | Human neuronal cell line |
WO1998057663A1 (en) * | 1997-06-17 | 1998-12-23 | University Technology Corporation | Neural transplantation for treating neurological disease |
US6734015B1 (en) * | 1997-07-04 | 2004-05-11 | University Of Utah Research Foundation | Isolation of lineage-restricted neuronal precursors |
AU755657B2 (en) * | 1997-07-04 | 2002-12-19 | University Of Utah Research Foundation | Lineage-restricted neuronal precursors |
US6235527B1 (en) * | 1997-11-29 | 2001-05-22 | University Of Utah Research Foundation | Lineage restricted glial precursors from the central nervous system |
US5968829A (en) * | 1997-09-05 | 1999-10-19 | Cytotherapeutics, Inc. | Human CNS neural stem cells |
WO1999013726A1 (en) * | 1997-09-19 | 1999-03-25 | University Of South Florida | Neuronal cells and an immunosuppressant and anti-inflammatory factor |
WO2000006700A1 (en) * | 1998-07-29 | 2000-02-10 | Layton Bioscience, Inc. | Production and use of dopaminergic cells to treat dopaminergic deficiencies |
EP1949904A3 (de) | 1999-04-27 | 2008-08-06 | Layton Bioscience, Inc. | Zelltherapie für chronische Apoplexie |
US20030211085A1 (en) * | 2000-03-16 | 2003-11-13 | Sanberg Paul R. | Cell therapy for chronic stroke |
DE19928210B4 (de) * | 1999-06-19 | 2005-08-18 | Neuroprogen Gmbh Leipzig | Neuronales Zellmaterial und Verfahren zu dessen Herstellung |
US6749850B1 (en) | 1999-08-18 | 2004-06-15 | The General Hospital Corporation | Methods, compositions and kits for promoting recovery from damage to the central nervous system |
WO2001053503A1 (en) * | 2000-01-18 | 2001-07-26 | Cornell Research Foundation, Inc. | Neural progenitor cells from hippocampal tissue and a method for isolating and purifying them |
DE60123937T2 (de) | 2000-04-13 | 2007-06-14 | The Board of Regents, The University of Texas System, Austin | Behandlung von neuro-degenerativen gastrointestinalkrankheiten durch impantation von neuronalen stammzellen und/oder deren nachkommen in gastrointestinalorgane |
US7037493B2 (en) | 2000-05-01 | 2006-05-02 | Cornell Research Foundation, Inc. | Method of inducing neuronal production in the brain and spinal cord |
US6897061B1 (en) | 2000-06-16 | 2005-05-24 | Spinal Cord Society | Transdifferentiation of glial cells |
JP4031901B2 (ja) * | 2000-07-19 | 2008-01-09 | 株式会社東芝 | 固体撮像装置 |
ATE420869T1 (de) * | 2000-10-05 | 2009-01-15 | Takeda Pharmaceutical | Promotoren zur proliferation und differenzierung von stammzellen und/oder vorstufen von neuronenzellen |
WO2002063938A2 (en) * | 2000-12-05 | 2002-08-22 | Layton Bioscience Inc. | Production and use of dopaminergic cells to treat dopaminergic deficiencies |
EP1397485A2 (de) * | 2001-06-05 | 2004-03-17 | Thomas Rohrmeier | Zellpopulationen zur auffindung neuronaler targets und potentieller wirkstoffe |
US7576065B2 (en) * | 2002-02-15 | 2009-08-18 | Cornell Research Foundation, Inc. | Enhancing neurotrophin-induced neurogenesis by endogenous neural progenitor cells by concurrent overexpression of brain derived neurotrophic factor and an inhibitor of a pro-gliogenic bone morphogenetic protein |
GB0217981D0 (en) * | 2002-08-02 | 2002-09-11 | Univ Durham | Method for preparation, purification and differentiation of neurospheres from mammalian stem cells |
US8293488B2 (en) | 2002-12-09 | 2012-10-23 | Neuralstem, Inc. | Method for screening neurogenic agents |
EP1576134B1 (de) * | 2002-12-09 | 2013-03-06 | Judith Kelleher-Andersson | Verfahren zur entdeckung neurogener wirkstoffe |
WO2004098621A1 (en) * | 2003-04-17 | 2004-11-18 | U.S. Department Of Veterans Affairs | Isolated/cloned human nt2 cell lines expressing serotonin and gaba |
FR2859219B1 (fr) * | 2003-09-02 | 2005-10-14 | Alain Privat | Procede de production de neurones a partir de cellules d'une lignee cellulaire |
AU2005252434B2 (en) | 2004-06-09 | 2011-11-24 | The University Court Of The University Of Edinburgh | Neural stem cells |
US7663614B2 (en) * | 2004-10-05 | 2010-02-16 | Jeff Maynard | Method for storing and comparing computer generated lines |
EP1814979B1 (de) * | 2004-11-17 | 2015-04-08 | Neuralstem, Inc. | Transplantation menschlicher nervenzellen zur behandlung neurodegenerativer erkrankungen |
CA2578493A1 (en) * | 2007-02-21 | 2008-08-21 | Prince Henry's Institute Of Medical Research | A role for sry in parkinson's disease |
US20090136461A1 (en) * | 2007-11-28 | 2009-05-28 | Moon Suk Kim | Neuronal differentiation method of adult stem cells using small molecules |
EP3187581A1 (de) | 2008-12-23 | 2017-07-05 | BOCO Silicon Valley, Inc. | Zielpopulationen von oligodendrozyten-vorläuferzellen und verfahren zur herstellung und verwendung davon |
US9101570B1 (en) | 2009-02-13 | 2015-08-11 | Endocellutions, Inc. | Adult and neonatal stem cell therapy to treat diabetes through the repair of the gastrointestinal tract |
WO2012016049A1 (en) | 2010-07-28 | 2012-02-02 | Neuralstem, Inc. | Methods for treating and/or reversing neurodegenerative diseases and/or disorders |
EP3209306B1 (de) | 2014-10-20 | 2024-04-24 | Neuralstem, Inc. | Stabile neurale stammzellen mit einem exogenen polynukleotid zur codierung eines wachstumsfaktors und verfahren zur verwendung davon |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4829000A (en) * | 1985-08-30 | 1989-05-09 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Reconstituted basement membrane complex with biological activity |
US5032407A (en) * | 1987-01-16 | 1991-07-16 | Ohio University Edison Animal Biotechnology Center | Gene transfer using transformed, neodetermined, embryonic cells |
US5270191A (en) * | 1988-04-12 | 1993-12-14 | Massachusetts Institute Of Technology | Method for manipulation of the cell types of eukaryotes |
FR2645866B1 (fr) * | 1989-04-17 | 1991-07-05 | Centre Nat Rech Scient | Nouvelles lipopolyamines, leur preparation et leur emploi |
US5180820A (en) * | 1989-08-30 | 1993-01-19 | Barde Yves Alain | Brain-derived neurotrophic factor |
US5175103A (en) * | 1991-10-21 | 1992-12-29 | Trustees Of University Of Pennsylvania | Preparation of pure cultures of post-mitotic human neurons |
-
1991
- 1991-10-21 US US07/780,715 patent/US5175103A/en not_active Expired - Lifetime
-
1992
- 1992-08-05 DK DK92917219T patent/DK0620847T3/da active
- 1992-08-05 CA CA002120730A patent/CA2120730A1/en not_active Abandoned
- 1992-08-05 AT AT92917219T patent/ATE165617T1/de not_active IP Right Cessation
- 1992-08-05 JP JP50765893A patent/JP3169611B2/ja not_active Expired - Fee Related
- 1992-08-05 DE DE69225332T patent/DE69225332T2/de not_active Expired - Fee Related
- 1992-08-05 ES ES92917219T patent/ES2117672T3/es not_active Expired - Lifetime
- 1992-08-05 EP EP92917219A patent/EP0620847B1/de not_active Expired - Lifetime
- 1992-08-05 WO PCT/US1992/006540 patent/WO1993008266A1/en active IP Right Grant
-
1996
- 1996-06-10 US US08/660,723 patent/US5654189A/en not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10259617A1 (de) * | 2002-12-18 | 2004-07-08 | Cardion Ag | Verfahren zur Inhibierung des Proliferationspotentials von Zellpopulationen und Geweben |
Also Published As
Publication number | Publication date |
---|---|
EP0620847B1 (de) | 1998-04-29 |
JP3169611B2 (ja) | 2001-05-28 |
EP0620847A1 (de) | 1994-10-26 |
EP0620847A4 (de) | 1995-03-29 |
US5175103A (en) | 1992-12-29 |
JPH07502645A (ja) | 1995-03-23 |
ES2117672T3 (es) | 1998-08-16 |
ATE165617T1 (de) | 1998-05-15 |
US5654189A (en) | 1997-08-05 |
DE69225332D1 (de) | 1998-06-04 |
CA2120730A1 (en) | 1993-04-29 |
WO1993008266A1 (en) | 1993-04-29 |
DK0620847T3 (da) | 1998-10-07 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8328 | Change in the person/name/address of the agent |
Representative=s name: VONNEMANN, KLOIBER & KOLLEGEN, 80796 MUENCHEN |
|
8339 | Ceased/non-payment of the annual fee |