DE69434377D1 - Verfahren zur herstellung von polyhydroxybutyrat und verwandten polyhydroxyalkanoaten in den plastiden hoeherer pflanzen - Google Patents

Verfahren zur herstellung von polyhydroxybutyrat und verwandten polyhydroxyalkanoaten in den plastiden hoeherer pflanzen

Info

Publication number
DE69434377D1
DE69434377D1 DE69434377T DE69434377T DE69434377D1 DE 69434377 D1 DE69434377 D1 DE 69434377D1 DE 69434377 T DE69434377 T DE 69434377T DE 69434377 T DE69434377 T DE 69434377T DE 69434377 D1 DE69434377 D1 DE 69434377D1
Authority
DE
Germany
Prior art keywords
plants
plastid
hoeherer
polyhydroxybutyrate
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69434377T
Other languages
English (en)
Other versions
DE69434377T2 (de
Inventor
Roland Somerville
Christiane Nawrath
Yves Poirier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Michigan State University MSU
Original Assignee
Michigan State University MSU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Michigan State University MSU filed Critical Michigan State University MSU
Application granted granted Critical
Publication of DE69434377D1 publication Critical patent/DE69434377D1/de
Publication of DE69434377T2 publication Critical patent/DE69434377T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
DE69434377T 1993-08-17 1994-08-17 Verfahren zur herstellung von polyhydroxybutyrat und verwandten polyhydroxyalkanoaten in den plastiden hoeherer pflanzen Expired - Lifetime DE69434377T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US10819393A 1993-08-17 1993-08-17
US108193 1993-08-17
US08/254,357 US5610041A (en) 1991-07-19 1994-06-06 Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants
US254357 1994-06-06
PCT/US1994/009265 WO1995005472A2 (en) 1993-08-17 1994-08-17 Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants

Publications (2)

Publication Number Publication Date
DE69434377D1 true DE69434377D1 (de) 2005-06-23
DE69434377T2 DE69434377T2 (de) 2006-02-02

Family

ID=26805624

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69434377T Expired - Lifetime DE69434377T2 (de) 1993-08-17 1994-08-17 Verfahren zur herstellung von polyhydroxybutyrat und verwandten polyhydroxyalkanoaten in den plastiden hoeherer pflanzen

Country Status (9)

Country Link
US (1) US5610041A (de)
EP (1) EP0719342B1 (de)
JP (1) JPH09501832A (de)
AT (1) ATE295894T1 (de)
AU (1) AU7633094A (de)
DE (1) DE69434377T2 (de)
ES (1) ES2240965T3 (de)
HK (1) HK1013110A1 (de)
WO (1) WO1995005472A2 (de)

Families Citing this family (53)

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US5245023A (en) 1987-06-29 1993-09-14 Massachusetts Institute Of Technology Method for producing novel polyester biopolymers
US6083729A (en) * 1995-10-26 2000-07-04 Metabolix, Inc. Methods for isolating polyhydroxyalkanoates from plants
GB9522896D0 (en) * 1995-11-08 1996-01-10 Zeneca Ltd Polyester production
US5849894A (en) * 1995-11-29 1998-12-15 Monsanto Company Rhodospirillum rubrum poly-β-hydroxyalkanoate synthase
US6600029B1 (en) 1995-12-19 2003-07-29 Regents Of The University Of Minnesota Metabolic engineering of polyhydroxyalkanoate monomer synthases
US6946588B2 (en) 1996-03-13 2005-09-20 Monsanto Technology Llc Nucleic acid encoding a modified threonine deaminase and methods of use
US5959179A (en) * 1996-03-13 1999-09-28 Monsanto Company Method for transforming soybeans
US5958745A (en) * 1996-03-13 1999-09-28 Monsanto Company Methods of optimizing substrate pools and biosynthesis of poly-β-hydroxybutyrate-co-poly-β-hydroxyvalerate in bacteria and plants
GB9606187D0 (en) * 1996-03-23 1996-05-29 Inst Of Food Research Production of vanillin
US5750848A (en) * 1996-08-13 1998-05-12 Monsanto Company DNA sequence useful for the production of polyhydroxyalkanoates
JP3062459B2 (ja) * 1996-08-14 2000-07-10 理化学研究所 ポリエステル重合酵素遺伝子及びポリエステルの製造方法
US6117658A (en) * 1997-02-13 2000-09-12 James Madison University Methods of making polyhydroxyalkanoates comprising 4-hydroxybutyrate monomer units
US6143561A (en) 1997-06-30 2000-11-07 The Curators Of The University Of Missouri DNA encoding plastid pyruvate dehydrogenase and branched chain oxoacid dehydrogenase components
WO1999014313A2 (en) * 1997-09-19 1999-03-25 Metabolix, Inc. Biological systems for manufacture of polyhydroxylalkanoate polymers containing 4-hydroxyacids
WO1999035278A1 (en) * 1998-01-05 1999-07-15 Monsanto Company Biosynthesis of medium chain length polyhydroxyalkanoates
EP1045642B1 (de) 1998-01-07 2002-11-06 Metabolix, Inc. Tierfutterzusammensetzungen
DE69924830T2 (de) * 1998-01-19 2006-03-02 Lg Chemical Ltd. Gene von alcaligenes latus, die mit der biosynthese von polyhydroxyalkanoaten assoziiert sind.
AU750057B2 (en) * 1998-03-06 2002-07-11 Metabolix, Inc. Modification of fatty acid metabolism in plants
US6586658B1 (en) 1998-03-06 2003-07-01 Metabolix, Inc. Modification of fatty acid metabolism in plants
US6103956A (en) * 1998-03-31 2000-08-15 Regents Of The University Of Minnesota Polyhydroxyalkanoate synthesis in plants
US6143952A (en) * 1998-03-31 2000-11-07 Regents Of The University Of Minnesota Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase
ES2339725T3 (es) 1998-04-08 2010-05-24 Metabolix, Inc. Metodos para la separacion y purificacion de biopolimeros.
CA2726139A1 (en) 1998-05-22 1999-12-02 Metabolix, Inc. Polyhdroxyalkanoate biopolymer compositions
US6265202B1 (en) 1998-06-26 2001-07-24 Regents Of The University Of Minnesota DNA encoding methymycin and pikromycin
EP1100928B1 (de) * 1998-07-30 2006-02-01 Metabolix, Inc. Bifunktionelle enzyme zur biopolymerherstellung
MXPA01001111A (es) 1998-08-04 2002-04-24 Metabolix Inc Produccion de polihidroxialcanoato a partir de polioles.
US6160199A (en) * 1998-12-21 2000-12-12 The Procter & Gamble Company Absorbent articles comprising biodegradable PHA copolymers
US6077931A (en) * 1998-12-21 2000-06-20 The Procter & Gamble Company Biodegradable PHA copolymers
US6174990B1 (en) 1998-12-21 2001-01-16 The Procter & Gamble Company Films comprising biodegradable PHA copolymers
EP1159435A1 (de) * 1999-03-05 2001-12-05 Monsanto Technology LLC Multigen-expressionsvektoren zur biosynthese von produkten durch multienzym-biosynthesewege
AU3266800A (en) * 1999-03-15 2000-10-04 Her Majesty In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada Method for producing polyhydroxyalkanoates in recombinant organisms
ATE292188T1 (de) * 1999-05-12 2005-04-15 Metabolix Inc Verfahren zur reinigung von polyhydroxyalkanoaten
WO2000078985A1 (en) * 1999-06-24 2000-12-28 Metabolix, Inc. Plant multi-gene expression constructs
US6451564B1 (en) 1999-07-02 2002-09-17 Massachusetts Institute Of Technology Methods for producing L-isoleucine
DE60040125D1 (de) 1999-08-09 2008-10-16 Riken Wako Verfahren zur Transformierung von Pflanzen, transformierte Pflanzen und Verfahren zur Herstellung von Polyestern
US20020132308A1 (en) * 2000-08-24 2002-09-19 Mpep @ Page 300-M Novel constructs and their use in metabolic pathway engineering
US20030194784A1 (en) * 2001-04-17 2003-10-16 Sherman David H. DNA encoding methymycin and pikromycin
EP2216405A1 (de) 2002-05-03 2010-08-11 Monsanto Technology LLC Saatspezifische USP-Promotoren zur Expression von Genen in Pflanzen
US20030213013A1 (en) * 2002-05-07 2003-11-13 Caimi Perry G. Fructose polymer synthesis in monocot plastids
DE10240035A1 (de) * 2002-08-30 2004-03-11 Rehm, Bernd H.A., PD Dr.rer.nat. Biogene Polyester-Partikel definierter Größe mit funktionalisierten Oberflächen:Herstellungsverfahren und pharmazeutische Zubereitungen, in denen diese enthalten sind
CA2623705A1 (en) * 2005-09-27 2007-04-05 Bernd Helmut Adam Rehm Polymer particles and uses thereof
LT2821494T (lt) 2007-03-16 2017-04-25 Genomatica, Inc. 1,4-butandiolio ir jo pirmtakų biosintezės būdai ir kompozicijos
US7947483B2 (en) 2007-08-10 2011-05-24 Genomatica, Inc. Methods and organisms for the growth-coupled production of 1,4-butanediol
EP3514242A3 (de) * 2008-09-10 2019-08-28 Genomatica, Inc. Mikroorganismen zur herstellung von 1,4-butandiol
JP6133599B2 (ja) * 2009-06-04 2017-05-24 ゲノマチカ， インク． 発酵ブロスの成分を分離する方法
CN113528417A (zh) 2009-06-04 2021-10-22 基因组股份公司 生产1,4-丁二醇的微生物和相关方法
JP2013507145A (ja) * 2009-10-13 2013-03-04 ゲノマチカ， インク． １，４−ブタンジオール、４−ヒドロキシブタナール、４−ヒドロキシブチリル−ＣｏＡ、プトレシン及び関連化合物の生成のための微生物体並びに関連する方法
US8530210B2 (en) 2009-11-25 2013-09-10 Genomatica, Inc. Microorganisms and methods for the coproduction 1,4-butanediol and gamma-butyrolactone
CN102781901B (zh) 2010-02-11 2016-08-03 梅塔玻利克斯公司 用于从经遗传修饰的聚羟基链烷酸酯生物质制备单体组分的方法
US8637286B2 (en) * 2010-02-23 2014-01-28 Genomatica, Inc. Methods for increasing product yields
US8048661B2 (en) * 2010-02-23 2011-11-01 Genomatica, Inc. Microbial organisms comprising exogenous nucleic acids encoding reductive TCA pathway enzymes
CN111705028A (zh) 2012-06-04 2020-09-25 基因组股份公司 制造4-羟基丁酸酯、1，4-丁二醇和相关化合物的微生物和方法
US11306333B2 (en) * 2017-10-04 2022-04-19 Lanzatech, Inc. Wood-Ljungdahl microorganisms that produce polyhydroxybutyrate

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5250430A (en) * 1987-06-29 1993-10-05 Massachusetts Institute Of Technology Polyhydroxyalkanoate polymerase
US5229279A (en) * 1987-06-29 1993-07-20 Massachusetts Institute Of Technology Method for producing novel polyester biopolymers
US5245023A (en) * 1987-06-29 1993-09-14 Massachusetts Institute Of Technology Method for producing novel polyester biopolymers
DE69032713T2 (de) * 1989-07-10 1999-04-08 Massachusetts Inst Technology Verfahren zur Herstellung von Polyester-Biopolymeren
GB9108756D0 (en) * 1991-04-24 1991-06-12 Ici Plc Production of polyalkanoate in plants
GB9115245D0 (en) * 1991-07-16 1991-08-28 Ici Plc Production of polyalkanoate
BR9206297A (pt) * 1991-07-19 1995-10-17 Univ Michigan State Plantas transgênicas produzindo poli-hidroxialcanoatos
GB9223332D0 (en) * 1992-11-06 1992-12-23 Ici Plc Production of polyhydroxyalkanoate in plants
GB9306490D0 (en) * 1993-03-29 1993-05-19 Zeneca Ltd Plant gene specifying acetyl,coenzyme a carboxylase and transformed plants containing same

Also Published As

Publication number Publication date
JPH09501832A (ja) 1997-02-25
WO1995005472A2 (en) 1995-02-23
ES2240965T3 (es) 2005-10-16
AU7633094A (en) 1995-03-14
US5610041A (en) 1997-03-11
WO1995005472A3 (en) 1995-03-30
HK1013110A1 (en) 1999-08-13
EP0719342A1 (de) 1996-07-03
ATE295894T1 (de) 2005-06-15
EP0719342B1 (de) 2005-05-18
DE69434377T2 (de) 2006-02-02

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