DE69502421T2 - Verfahren zum Nachweis von DNS-Strängen unter Verwendung von im Infrarot fluoreszierenden Labeln - Google Patents
Verfahren zum Nachweis von DNS-Strängen unter Verwendung von im Infrarot fluoreszierenden LabelnInfo
- Publication number
- DE69502421T2 DE69502421T2 DE69502421T DE69502421T DE69502421T2 DE 69502421 T2 DE69502421 T2 DE 69502421T2 DE 69502421 T DE69502421 T DE 69502421T DE 69502421 T DE69502421 T DE 69502421T DE 69502421 T2 DE69502421 T2 DE 69502421T2
- Authority
- DE
- Germany
- Prior art keywords
- detection
- fluorescent labels
- dna strands
- infrared fluorescent
- infrared
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/56—Ring systems containing three or more rings
- C07D209/58—[b]- or [c]-condensed
- C07D209/60—Naphtho [b] pyrroles; Hydrogenated naphtho [b] pyrroles
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/0066—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain being part of a carbocyclic ring,(e.g. benzene, naphtalene, cyclohexene, cyclobutenene-quadratic acid)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
- G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/80—Fluorescent dyes, e.g. rhodamine
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Electrochemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Indole Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/204,627 US5571388A (en) | 1984-03-29 | 1994-03-01 | Sequencing near infrared and infrared fluorescense labeled DNA for detecting using laser diodes and suitable labels thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69502421D1 DE69502421D1 (de) | 1998-06-18 |
DE69502421T2 true DE69502421T2 (de) | 1999-01-07 |
Family
ID=22758717
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69502421T Expired - Fee Related DE69502421T2 (de) | 1994-03-01 | 1995-02-28 | Verfahren zum Nachweis von DNS-Strängen unter Verwendung von im Infrarot fluoreszierenden Labeln |
Country Status (6)
Country | Link |
---|---|
US (2) | US5571388A (de) |
EP (1) | EP0670374B1 (de) |
JP (1) | JPH0847400A (de) |
AU (1) | AU707452B2 (de) |
CA (1) | CA2143559A1 (de) |
DE (1) | DE69502421T2 (de) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19921234B4 (de) * | 1999-05-07 | 2005-07-28 | Few Chemicals Gmbh | Cyanin-Farbstoffe |
Families Citing this family (74)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4729947A (en) * | 1984-03-29 | 1988-03-08 | The Board Of Regents Of The University Of Nebraska | DNA sequencing |
US5863403A (en) * | 1984-03-29 | 1999-01-26 | The Board Of Regents Of The University Of Nebraska | Digital DNA typing |
US6086737A (en) * | 1984-03-29 | 2000-07-11 | Li-Cor, Inc. | Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes and suitable labels therefor |
US5571388A (en) * | 1984-03-29 | 1996-11-05 | Li-Cor, Inc. | Sequencing near infrared and infrared fluorescense labeled DNA for detecting using laser diodes and suitable labels thereof |
US6207421B1 (en) | 1984-03-29 | 2001-03-27 | Li-Cor, Inc. | DNA sequencing and DNA terminators |
US6004446A (en) * | 1984-03-29 | 1999-12-21 | Li-Cor, Inc. | DNA Sequencing |
US5360523A (en) * | 1984-03-29 | 1994-11-01 | Li-Cor, Inc. | DNA sequencing |
US6593148B1 (en) * | 1994-03-01 | 2003-07-15 | Li-Cor, Inc. | Cyanine dye compounds and labeling methods |
JP3515646B2 (ja) * | 1995-09-18 | 2004-04-05 | 大塚電子株式会社 | マルチキャピラリ電気泳動装置 |
US6027709A (en) * | 1997-01-10 | 2000-02-22 | Li-Cor Inc. | Fluorescent cyanine dyes |
CA2281205A1 (en) | 1997-02-12 | 1998-08-13 | Eugene Y. Chan | Methods and products for analyzing polymers |
US6361942B1 (en) * | 1998-03-24 | 2002-03-26 | Boston Probes, Inc. | Method, kits and compositions pertaining to detection complexes |
US20030235846A1 (en) * | 1998-04-08 | 2003-12-25 | Terpetschnig Ewald A. | Luminescent compounds |
US7250517B2 (en) * | 1998-04-08 | 2007-07-31 | Ewald A. Terpetschnig | Luminescent compounds |
US7411068B2 (en) * | 1998-04-08 | 2008-08-12 | Terpetschnig Ewald A | Luminescent compounds |
JP3613032B2 (ja) * | 1998-10-26 | 2005-01-26 | 株式会社日立製作所 | キャピラリーアレイ電気泳動装置 |
WO2000034394A2 (de) * | 1998-12-05 | 2000-06-15 | Otto Samuel Wolfbeis | Pyridin- und chinolin-farbstoffe als marker für biomoleküle, polymere, arzneistoffe und partikel |
US6312906B1 (en) | 1999-01-15 | 2001-11-06 | Imperial College Innovations, Ltd. | Immobilized nucleic acid hybridization reagent and method |
US6628385B1 (en) * | 1999-02-05 | 2003-09-30 | Axon Instruments, Inc. | High efficiency, large field scanning microscope |
DE19911421A1 (de) * | 1999-03-11 | 2000-10-05 | Dyomics Gmbh | Laser-kompatible NIR-Marker-Farbstoffe |
US6605434B1 (en) * | 1999-03-16 | 2003-08-12 | Human Genome Sciences, Inc. | Direct bacterial lysate sequencing |
WO2001002374A1 (en) * | 1999-07-06 | 2001-01-11 | Surromed, Inc. | Bridged fluorescent dyes, their preparation and their use in assays |
DE19937024A1 (de) * | 1999-08-05 | 2001-02-08 | Bayer Ag | Verwendung von Acylsulfonamido substituierten Polymethin-Farbstoffen als Fluoreszenz-Farbstoffe und/oder Marker |
US6569685B1 (en) * | 1999-10-05 | 2003-05-27 | The Molecular Sciences Institute, Inc. | Protein fingerprint system and related methods |
DE10025820C2 (de) * | 2000-05-23 | 2003-06-26 | Dyomics Gmbh | Stabile NIR-Marker-Farbstoffe auf der Basis von Benzopyrylium-Polymethinen |
DE60131194T2 (de) * | 2000-07-07 | 2008-08-07 | Visigen Biotechnologies, Inc., Bellaire | Sequenzbestimmung in echtzeit |
DE60116510T2 (de) | 2000-09-19 | 2006-07-13 | Li-Cor, Inc., Lincoln | Cyaninfarbstoffe |
US7597878B2 (en) * | 2000-09-19 | 2009-10-06 | Li-Cor, Inc. | Optical fluorescent imaging |
US6335450B1 (en) | 2000-11-09 | 2002-01-01 | Beckman Coulter, Inc. | Efficient cyclic-bridged cyanine dyes |
US6331632B1 (en) | 2000-11-09 | 2001-12-18 | Beckman Coulter, Inc. | Cyanine dye phosphoramidites |
WO2002044425A2 (en) | 2000-12-01 | 2002-06-06 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
EP1221465A1 (de) * | 2001-01-03 | 2002-07-10 | Innosense S.r.l. | Symmetrische, monofunktionalisierte Polymethinfarbstoffe als Markierungsreagenzien |
EP1223197B1 (de) * | 2001-01-03 | 2012-08-08 | Visen Medical, Inc. | Symmetrische, monofunktionalisierte Polymethinfarbstoffe als Markierungsreagenzien |
AU2002306620A1 (en) * | 2001-02-28 | 2002-09-12 | Stratagene | Fluorescent dye |
US7189366B2 (en) * | 2001-05-25 | 2007-03-13 | The United States Of America As Represented By The Secretary Of The Interior | Molecular tag reader |
US7668697B2 (en) | 2006-02-06 | 2010-02-23 | Andrei Volkov | Method for analyzing dynamic detectable events at the single molecule level |
US20030090012A1 (en) * | 2001-09-27 | 2003-05-15 | Allen Richard Charles | Methods of making polarization rotators and articles containing the polarization rotators |
US6838289B2 (en) * | 2001-11-14 | 2005-01-04 | Beckman Coulter, Inc. | Analyte detection system |
WO2003079015A1 (en) * | 2002-03-11 | 2003-09-25 | Visen Medical, Inc. | Optical imaging probes |
AU2003224912A1 (en) * | 2002-04-10 | 2003-10-27 | Igor Borovoy | Dyes and fluorescent compounds |
WO2004003510A2 (en) * | 2002-07-01 | 2004-01-08 | Guava Technologies, Inc. | Fluorescent dyes, energy transfer couples and methods |
US20090186343A1 (en) * | 2003-01-28 | 2009-07-23 | Visigen Biotechnologies, Inc. | Methods for preparing modified biomolecules, modified biomolecules and methods for using same |
US7148043B2 (en) * | 2003-05-08 | 2006-12-12 | Bio-Rad Laboratories, Inc. | Systems and methods for fluorescence detection with a movable detection module |
US7125644B2 (en) * | 2003-08-11 | 2006-10-24 | Hewlett-Packard Development Company, L.P. | Systems and methods for storing data on an optical disk |
US20070298507A1 (en) * | 2004-02-23 | 2007-12-27 | Tetsuo Nagano | Fluorescent Probe |
US7312326B2 (en) * | 2004-04-09 | 2007-12-25 | Beckman Coulter, Inc. | Fluorescent labeled nucleotide derivatives |
US7230117B2 (en) * | 2004-07-12 | 2007-06-12 | Beckman Coulter, Inc. | Stable cyanine dye phosphoramidites |
WO2006089268A2 (en) * | 2005-02-18 | 2006-08-24 | The University Of North Carolina At Chapel Hill | Gene and cognate protein profiles and methods to determine connective tissue markers in normal and pathologic conditions |
US7198834B2 (en) | 2005-03-22 | 2007-04-03 | Hewlett-Packard Development Company, L.P. | Imaging media including interference layer for generating human-readable marking on optical media |
US20060281100A1 (en) * | 2005-06-14 | 2006-12-14 | Shen Gene G | Thiotriphosphate nucleotide dye terminators |
US8227621B2 (en) * | 2005-06-30 | 2012-07-24 | Li-Cor, Inc. | Cyanine dyes and methods of use |
ATE535525T1 (de) * | 2006-03-31 | 2011-12-15 | Wako Pure Chem Ind Ltd | Cyaninfarbstoff vom pyrazoltyp |
US20070281363A1 (en) * | 2006-04-13 | 2007-12-06 | Ewald Terpetschnig | Luminescent compounds |
US20080076188A1 (en) * | 2006-06-19 | 2008-03-27 | Patsenker Leonid D | Luminescent compounds |
US20080241938A1 (en) * | 2006-07-20 | 2008-10-02 | Visigen Biotechnologies, Inc. | Automated synthesis or sequencing apparatus and method for making and using same |
US20080091005A1 (en) * | 2006-07-20 | 2008-04-17 | Visigen Biotechnologies, Inc. | Modified nucleotides, methods for making and using same |
US20080241951A1 (en) * | 2006-07-20 | 2008-10-02 | Visigen Biotechnologies, Inc. | Method and apparatus for moving stage detection of single molecular events |
CA2695117A1 (en) * | 2006-08-03 | 2008-02-07 | Beth Israel Deaconess Medical Center, Inc. | Dyes and precursors and conjugates thereof |
US20090320908A1 (en) * | 2008-06-27 | 2009-12-31 | Sunpower Corp. | Photovoltaic module with drainage frame |
US8663990B2 (en) * | 2009-04-28 | 2014-03-04 | Wako Pure Chemical Industries, Ltd. | Measurement method utilizing internal standard substance |
EP2426123B1 (de) | 2009-04-28 | 2014-08-06 | Wako Pure Chemical Industries, Ltd. | Pyrayzolbasierter cyaninfarbstoff mit quartärem ammoniumkation |
WO2010138738A1 (en) | 2009-05-27 | 2010-12-02 | Lumicell Diagnostics, Inc. | Methods and systems for spatially identifying abnormal cells |
US9155471B2 (en) | 2009-05-27 | 2015-10-13 | Lumicell, Inc'. | Methods and systems for spatially identifying abnormal cells |
CN103038629B (zh) * | 2010-05-31 | 2015-07-08 | 卫材R&D管理有限公司 | 淋巴结成像用荧光探针 |
WO2012054749A1 (en) | 2010-10-20 | 2012-04-26 | Li-Cor, Inc. | Cyanine dyes and their conjugates |
US9314304B2 (en) | 2010-12-08 | 2016-04-19 | Lumicell, Inc. | Methods and system for image guided cell ablation with microscopic resolution |
KR20160037834A (ko) | 2013-03-14 | 2016-04-06 | 루미셀, 아이엔씨. | 의료 이미징 장치 및 사용 방법 |
EP2970674B1 (de) * | 2013-03-15 | 2018-12-12 | VisEn Medical, Inc. | 4,4-disubstituierte cyclohexyl-verbrückte heptamethin-cyanin-farbstoffe und verwendungen davon |
CN106008481A (zh) * | 2016-05-19 | 2016-10-12 | 广州中医药大学 | 一种靶向肿瘤细胞的黄酮类化合物及其制备方法 |
EP3655408B1 (de) * | 2017-08-24 | 2021-07-14 | The U.S.A. As Represented By The Secretary, Department Of Health And Human Services | Konformationale einschränkung von cyaninfluorophoren im fernrot- und nahinfrarotbereich |
CA3094902A1 (en) | 2018-03-21 | 2019-09-26 | Ecole Polytechnique Federale De Lausanne (Epfl) | Azacyanine dyes and use thereof |
EP4190795A1 (de) * | 2020-07-30 | 2023-06-07 | FUJIFILM Corporation | Verbindung und markierte biologische substanz damit |
CN112239603B (zh) * | 2020-10-20 | 2022-06-21 | 安徽师范大学 | Ir-790及其制备方法、敏化上转换纳米探针及其制备方法和过氧亚硝酸盐的检测方法 |
WO2022163807A1 (ja) * | 2021-01-29 | 2022-08-04 | 国立大学法人北海道大学 | 光音響イメージング剤 |
Family Cites Families (63)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3423207A (en) * | 1964-09-01 | 1969-01-21 | Eastman Kodak Co | Solubilized styryl dyes |
US3873432A (en) * | 1972-10-30 | 1975-03-25 | Louis Israel | Continuous preparative electrophoresis method |
US4166105A (en) * | 1973-07-30 | 1979-08-28 | Block Engineering, Inc. | Dye tagged reagent |
US3969218A (en) * | 1975-02-11 | 1976-07-13 | The United States Of America As Represented By The United States Energy Research And Development Administration | Elution electrophoresis |
US4138551A (en) * | 1975-03-18 | 1979-02-06 | Ciba-Geigy Ag | Spectral sensitization of photographic material and new spectral sensitizers |
GB1529202A (en) * | 1976-02-20 | 1978-10-18 | Ciba Geigy Ag | Spectral sensitising dyes |
NL182822C (nl) * | 1976-05-18 | 1988-05-16 | Roskam Willem Gerrit | Preparatief elektroforese-apparaat. |
US4284491A (en) * | 1978-11-13 | 1981-08-18 | C. Desaga Gmbh Nachf. Erich Fecht | Apparatus for electrophoresis |
US4351709A (en) * | 1979-02-28 | 1982-09-28 | Goetz Philip J | Method of obtaining the mean electrophoretic mobility of particles |
JPS55116259A (en) * | 1979-03-01 | 1980-09-06 | Fuji Photo Film Co Ltd | Microimmunoassay method |
US4256834A (en) * | 1979-04-09 | 1981-03-17 | Syva Company | Fluorescent scavenger particle immunoassay |
DE3170135D1 (en) * | 1980-09-02 | 1985-05-30 | Fuji Photo Film Co Ltd | Method for immunochemical measurement of trace components |
JPS5747493A (en) * | 1980-09-02 | 1982-03-18 | Fuji Photo Film Co Ltd | Method of determining a trace amount of enzyme |
JPS5745460A (en) * | 1980-09-02 | 1982-03-15 | Fuji Photo Film Co Ltd | Inspection sheet for measuring trace component and inspecting method using said sheet |
US4711955A (en) * | 1981-04-17 | 1987-12-08 | Yale University | Modified nucleotides and methods of preparing and using same |
US4375401A (en) * | 1981-05-20 | 1983-03-01 | Nicholas Catsimpoolas | Electrophoresis system |
FR2519005B1 (fr) * | 1981-12-29 | 1985-10-25 | Pasteur Institut | Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn |
US5241060A (en) * | 1982-06-23 | 1993-08-31 | Enzo Diagnostics, Inc. | Base moiety-labeled detectable nucleatide |
US4948882A (en) * | 1983-02-22 | 1990-08-14 | Syngene, Inc. | Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis |
US5171534A (en) * | 1984-01-16 | 1992-12-15 | California Institute Of Technology | Automated DNA sequencing technique |
US5360523A (en) * | 1984-03-29 | 1994-11-01 | Li-Cor, Inc. | DNA sequencing |
US5571388A (en) * | 1984-03-29 | 1996-11-05 | Li-Cor, Inc. | Sequencing near infrared and infrared fluorescense labeled DNA for detecting using laser diodes and suitable labels thereof |
US5230781A (en) * | 1984-03-29 | 1993-07-27 | Li-Cor, Inc. | Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes |
JPS62123454A (ja) * | 1985-08-08 | 1987-06-04 | Fuji Photo Film Co Ltd | ハロゲン化銀写真感光材料 |
DE3529478A1 (de) * | 1985-08-16 | 1987-02-19 | Boehringer Mannheim Gmbh | 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung |
US4707235A (en) * | 1985-10-16 | 1987-11-17 | Pharmacia, Inc. | Electrophoresis method and apparatus having continuous detection means |
US4910300A (en) * | 1985-12-11 | 1990-03-20 | Chiron Corporation | Method for making nucleic acid probes |
US4855225A (en) * | 1986-02-07 | 1989-08-08 | Applied Biosystems, Inc. | Method of detecting electrophoretically separated oligonucleotides |
US4830786A (en) * | 1986-02-27 | 1989-05-16 | Syntex (U.S.A.) Inc. | Squaraine dyes |
US5039818A (en) * | 1986-02-27 | 1991-08-13 | Syntex (U.S.A.) Inc. | Squaraine dye |
US5268486A (en) * | 1986-04-18 | 1993-12-07 | Carnegie-Mellon Unversity | Method for labeling and detecting materials employing arylsulfonate cyanine dyes |
US5091519A (en) * | 1986-05-01 | 1992-02-25 | Amoco Corporation | Nucleotide compositions with linking groups |
US4811218A (en) * | 1986-06-02 | 1989-03-07 | Applied Biosystems, Inc. | Real time scanning electrophoresis apparatus for DNA sequencing |
US5047519A (en) * | 1986-07-02 | 1991-09-10 | E. I. Du Pont De Nemours And Company | Alkynylamino-nucleotides |
US5242796A (en) * | 1986-07-02 | 1993-09-07 | E. I. Du Pont De Nemours And Company | Method, system and reagents for DNA sequencing |
CA1340806C (en) * | 1986-07-02 | 1999-11-02 | James Merrill Prober | Method, system and reagents for dna sequencing |
US5151507A (en) * | 1986-07-02 | 1992-09-29 | E. I. Du Pont De Nemours And Company | Alkynylamino-nucleotides |
US4810348A (en) * | 1987-03-16 | 1989-03-07 | Helena Laboratories Corporation | Automatic electrophoresis apparatus and method |
US4833332A (en) * | 1987-06-12 | 1989-05-23 | E. I. Du Pont De Nemours And Company | Scanning fluorescent detection system |
JPH01238548A (ja) * | 1988-03-18 | 1989-09-22 | Agency Of Ind Science & Technol | ナフタレン―1,4,5,8―テトラカルボン酸テトラアルキルエステルの製造方法 |
US4927265A (en) * | 1988-04-29 | 1990-05-22 | 501 Microphoretic Systems, Inc. | Detector for fluorescence and absorption spectroscopy |
DE3912046B4 (de) * | 1988-09-02 | 2004-03-25 | Carnegie Mellon University | Verfahren zum Markieren einer Komponente einer wäßrigen Flüssigkeit und lumineszenzphotostabiles Reaktionsprodukt |
US4981977A (en) * | 1989-06-09 | 1991-01-01 | Carnegie-Mellon University | Intermediate for and fluorescent cyanine dyes containing carboxylic acid groups |
US5366860A (en) * | 1989-09-29 | 1994-11-22 | Applied Biosystems, Inc. | Spectrally resolvable rhodamine dyes for nucleic acid sequence determination |
US5188934A (en) * | 1989-11-14 | 1993-02-23 | Applied Biosystems, Inc. | 4,7-dichlorofluorescein dyes as molecular probes |
US5375606A (en) * | 1990-02-28 | 1994-12-27 | Zynaxis, Inc. | Bio-analytical separation method |
US5312921A (en) * | 1990-03-14 | 1994-05-17 | Regents Of The University Of California | Dyes designed for high sensitivity detection of double-stranded DNA |
US5326692B1 (en) * | 1992-05-13 | 1996-04-30 | Molecular Probes Inc | Fluorescent microparticles with controllable enhanced stokes shift |
JPH0743353B2 (ja) * | 1990-05-31 | 1995-05-15 | 株式会社島津製作所 | 蛍光検出型ゲル電気泳動装置 |
US5187085A (en) * | 1990-09-28 | 1993-02-16 | Applied Biosystems, Inc. | Nucleic acid sequence analysis with nucleoside-5'-o-(1-thiotriphosphates) |
US5276143A (en) * | 1990-12-21 | 1994-01-04 | E. I. Du Pont De Nemours And Company | Dideoxyfructonucleosides and deoxyfructonucleotides |
JP3285609B2 (ja) * | 1991-06-21 | 2002-05-27 | キヤノン株式会社 | 標識複合体、及びそれを用いる分析法 |
CA2119126C (en) * | 1991-09-16 | 1996-09-03 | Stephen T. Yue | Dimers of unsymmetrical cyanine dyes |
US5321130A (en) * | 1992-02-10 | 1994-06-14 | Molecular Probes, Inc. | Unsymmetrical cyanine dyes with a cationic side chain |
EP0628051B1 (de) * | 1992-02-12 | 2003-07-02 | Chromagen, Inc. | Verwendungen von fluoreszierenden n-nukleosiden und dessen analogen |
US5403708A (en) * | 1992-07-06 | 1995-04-04 | Brennan; Thomas M. | Methods and compositions for determining the sequence of nucleic acids |
US5438131A (en) * | 1992-09-16 | 1995-08-01 | Bergstrom; Donald E. | 3-nitropyrrole nucleoside |
JP3260884B2 (ja) * | 1993-02-05 | 2002-02-25 | キヤノン株式会社 | 標識複合体、それを用いる分析法、生体関連物質の検出および定量の方法 |
US5410030A (en) * | 1993-04-05 | 1995-04-25 | Molecular Probes, Inc. | Dimers of unsymmetrical cyanine dyes containing pyridinium moieties |
US5534416A (en) * | 1993-04-13 | 1996-07-09 | Molecular Probes, Inc. | Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes |
DE4326466A1 (de) * | 1993-08-06 | 1995-02-09 | Boehringer Mannheim Gmbh | Infrarot-Farbstoff-markierte Nucleotide und ihre Verwendung in der Nucleinsäure-Detektion |
FR2709761B1 (fr) * | 1993-09-10 | 1995-11-24 | Pasteur Institut | Procédé de détection de molécules contenant des mésappariements nucléotidiques et de localisation de ces mésappariements, et application à la détection de substitutions ou de délétions de bases . |
AU3008595A (en) * | 1994-06-30 | 1996-01-25 | Biometric Imaging, Inc. | N-heteroaromatic ion and iminium ion substituted cyanine dyes for use as fluorescence labels |
-
1994
- 1994-03-01 US US08/204,627 patent/US5571388A/en not_active Expired - Fee Related
-
1995
- 1995-02-28 DE DE69502421T patent/DE69502421T2/de not_active Expired - Fee Related
- 1995-02-28 EP EP95250047A patent/EP0670374B1/de not_active Expired - Lifetime
- 1995-02-28 CA CA002143559A patent/CA2143559A1/en not_active Abandoned
- 1995-03-01 JP JP7042169A patent/JPH0847400A/ja active Pending
- 1995-03-01 AU AU13582/95A patent/AU707452B2/en not_active Ceased
-
1996
- 1996-08-30 US US08/706,004 patent/US5800995A/en not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19921234B4 (de) * | 1999-05-07 | 2005-07-28 | Few Chemicals Gmbh | Cyanin-Farbstoffe |
Also Published As
Publication number | Publication date |
---|---|
EP0670374A1 (de) | 1995-09-06 |
AU707452B2 (en) | 1999-07-08 |
US5800995A (en) | 1998-09-01 |
DE69502421D1 (de) | 1998-06-18 |
AU1358295A (en) | 1995-09-07 |
US5571388A (en) | 1996-11-05 |
CA2143559A1 (en) | 1995-09-02 |
JPH0847400A (ja) | 1996-02-20 |
EP0670374B1 (de) | 1998-05-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE69502421D1 (de) | Verfahren zum Nachweis von DNS-Strängen unter Verwendung von im Infrarot fluoreszierenden Labeln | |
DE69520330D1 (de) | Verfahren zum Nachweis von spezifischen Nukleinsäuresequenzen | |
DE69706430D1 (de) | Verfahren zur sequenzierung von dna | |
DE69634696D1 (de) | Verfahren und testsystem zur hybridisierungsanalyse unter verwendung von peptidnukleinsäure-sonden | |
DE19782089T1 (de) | Verfahren zum Nachweisen von Punktmutationen in DNA unter Verwendung von Fluoreszenzenergieübergang | |
DE69719966D1 (de) | Verfahren zur gleichzeitigen erkennung von verschiedenen fluorophoren in der in-situ hybridisierung | |
DE69329318D1 (de) | Immunotestverfahren unter Verwendung von Mikropartikel enthaltenden, unterschiedlichen, nachweisbaren Substanzen | |
DE69803344T2 (de) | Vorrichtung zum nachweis von strahlung und verfahren zu ihrer herstellung | |
DE3682814D1 (de) | Verfahren zur guetepruefung von glaswaren unter verwendung von optischem strichnachweis. | |
DE69608152D1 (de) | Verfahren und System zur Wiederverwertung von Teppichen | |
DE69508921T2 (de) | Verfahren zum nachweis von staphylokokken | |
DE69916859D1 (de) | Verfahren zur beschichtung von textilprodukten | |
DE59909518D1 (de) | Verfahren zur Sicherung und Kennzeichnung von Produkten unter Verwendung von Mikropartikeln | |
DE69815268T2 (de) | Verfahren zur wiederverwertung von verkleidungsteilen | |
DE69529052T2 (de) | System zur erfassung von fehlzündungen und verfahren mit verbesserter signaltreue | |
DE69809592D1 (de) | Verfahren zur epoxidierung unter verwendung von co2-behandelten silberhaltigen katalysatoren | |
DE69907057D1 (de) | System und Verfahren zum Färben von Linsen | |
DE69602534T2 (de) | Verfahren zur verwendung von metallocenkatalysatoren | |
DE69715006D1 (de) | Verfahren zur sequenzierung von nukleinsäuren | |
DE69222710D1 (de) | Verfahren zum Nachweis von DNS-Basen mit Verwendung von Fluoreszenz | |
DE69533286D1 (de) | Neues glyceroglycophospholipid, antikörper dagegen und verfahren zur detektion von mycoplasma | |
DE69432333D1 (de) | Verfahren zur Wasserbehandlung unter Verwendung von 5-allyldibenzosuberenol | |
DE69319752D1 (de) | Verfahren zur identifizierung von analyten | |
DE69933962D1 (de) | Verfahren und Vorrichtung für das Reduzieren der Kompliziertheit von Farbkorrekturen unter Verwendung der Unterabtastung | |
DE69807778D1 (de) | Farbempfangselement und Verfahren unter Verwendung desselben |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |