DE69506393D1 - Hybridisation-ligitation-verfahren zum nachweis von spezifischen dns sequenzen - Google Patents

Hybridisation-ligitation-verfahren zum nachweis von spezifischen dns sequenzen

Info

Publication number
DE69506393D1
DE69506393D1 DE69506393T DE69506393T DE69506393D1 DE 69506393 D1 DE69506393 D1 DE 69506393D1 DE 69506393 T DE69506393 T DE 69506393T DE 69506393 T DE69506393 T DE 69506393T DE 69506393 D1 DE69506393 D1 DE 69506393D1
Authority
DE
Germany
Prior art keywords
ligitation
hybridisation
dna sequences
specific dna
detecting specific
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69506393T
Other languages
English (en)
Other versions
DE69506393T2 (de
Inventor
Richard Martinelli
John Arruda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Ciba Corning Diagnosys Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Corning Diagnosys Corp filed Critical Ciba Corning Diagnosys Corp
Publication of DE69506393D1 publication Critical patent/DE69506393D1/de
Application granted granted Critical
Publication of DE69506393T2 publication Critical patent/DE69506393T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6823Release of bound markers
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4712Cystic fibrosis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/682Signal amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6862Ligase chain reaction [LCR]
DE69506393T 1994-04-04 1995-04-03 Hybridisation-ligitation-verfahren zum nachweis von spezifischen dns sequenzen Expired - Lifetime DE69506393T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22261394A 1994-04-04 1994-04-04
PCT/IB1995/000226 WO1995027078A1 (en) 1994-04-04 1995-04-03 Hibridization-ligation assays for the detection of specific nucleic acid sequences

Publications (2)

Publication Number Publication Date
DE69506393D1 true DE69506393D1 (de) 1999-01-14
DE69506393T2 DE69506393T2 (de) 1999-07-01

Family

ID=22832958

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69506393T Expired - Lifetime DE69506393T2 (de) 1994-04-04 1995-04-03 Hybridisation-ligitation-verfahren zum nachweis von spezifischen dns sequenzen

Country Status (9)

Country Link
US (1) US5800994A (de)
EP (1) EP0754241B1 (de)
JP (1) JP3738910B2 (de)
AT (1) ATE174066T1 (de)
BR (1) BR9507343A (de)
CA (1) CA2186465A1 (de)
DE (1) DE69506393T2 (de)
PL (1) PL316611A1 (de)
WO (1) WO1995027078A1 (de)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6270961B1 (en) * 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
US6401267B1 (en) 1993-09-27 2002-06-11 Radoje Drmanac Methods and compositions for efficient nucleic acid sequencing
USRE43097E1 (en) 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
GB9507238D0 (en) * 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5888731A (en) * 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
US6309824B1 (en) * 1997-01-16 2001-10-30 Hyseq, Inc. Methods for analyzing a target nucleic acid using immobilized heterogeneous mixtures of oligonucleotide probes
US6297006B1 (en) * 1997-01-16 2001-10-02 Hyseq, Inc. Methods for sequencing repetitive sequences and for determining the order of sequence subfragments
EP0985142A4 (de) * 1997-05-23 2006-09-13 Lynx Therapeutics Inc System und gerät zur sequentiellen behandlung von analyten
WO1999009217A1 (en) * 1997-08-15 1999-02-25 Hyseq, Inc. Methods and compositions for detection or quantification of nucleic acid species
JP2002508190A (ja) * 1997-12-12 2002-03-19 ダイジーン・コーポレーション 万能収集媒質
AU754952B2 (en) 1998-06-24 2002-11-28 Illumina, Inc. Decoding of array sensors with microspheres
US5998175A (en) * 1998-07-24 1999-12-07 Lumigen, Inc. Methods of synthesizing and amplifying polynucleotides by ligation of multiple oligomers
US6232067B1 (en) * 1998-08-17 2001-05-15 The Perkin-Elmer Corporation Adapter directed expression analysis
US6180408B1 (en) 1998-08-21 2001-01-30 Washington University Fluorescence polarization in nucleic acid analysis
ATE234468T1 (de) 1998-09-18 2003-03-15 Massachusetts Inst Technology Biologische verwendungen von halbleitenden nanokristallen
US6620584B1 (en) * 1999-05-20 2003-09-16 Illumina Combinatorial decoding of random nucleic acid arrays
ATE318932T1 (de) * 1999-08-13 2006-03-15 Univ Yale Binär kodierte sequenzmarker
EP1136568A4 (de) 1999-10-04 2004-12-15 Olympus Optical Corp Ltd Methode zum ermitteln der nukleinsäure
US6569685B1 (en) * 1999-10-05 2003-05-27 The Molecular Sciences Institute, Inc. Protein fingerprint system and related methods
US7390675B2 (en) * 1999-10-15 2008-06-24 Christopher Feistel Multi-functional and configurable assay
US7371584B2 (en) * 1999-10-15 2008-05-13 Wavesence, Inc. Multi-functional and configurable assay
US6136549A (en) * 1999-10-15 2000-10-24 Feistel; Christopher C. systems and methods for performing magnetic chromatography assays
US6368801B1 (en) 2000-04-12 2002-04-09 Molecular Staging, Inc. Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase
CA2410950A1 (en) * 2000-05-30 2001-12-06 Hans-Michael Wenz Methods for detecting target nucleic acids using coupled ligation and amplification
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US6562575B1 (en) 2000-06-26 2003-05-13 Epicentre Technologies Corporation Analyte-specific assays based on formation of a replicase substrate
JP4755755B2 (ja) * 2000-12-15 2011-08-24 独立行政法人産業技術総合研究所 複数検査多重化用懸濁液およびその懸濁液を用いた複数検査多重化方法
US20040266021A9 (en) * 2001-01-26 2004-12-30 Andras Guttman Multicapillary fraction collection system and method
US20020168667A1 (en) * 2001-03-23 2002-11-14 Fuji Photo Film Co., Ltd. Antigen detecting agent and antigen detecting kit, antigen detecting apparatus and antigen detecting method using the same
US7077982B2 (en) * 2001-03-23 2006-07-18 Fuji Photo Film Co., Ltd. Molecular electric wire, molecular electric wire circuit using the same and process for producing the molecular electric wire circuit
US7018795B2 (en) * 2001-03-23 2006-03-28 Fuji Photo Film Co., Ltd. Hybridization probe and target nucleic acid detecting kit, target nucleic acid detecting apparatus and target nucleic acid detecting method using the same
US20020168756A1 (en) * 2001-03-23 2002-11-14 Fuji Photo Film Co., Ltd. Particle size variable reactor
US20030003476A1 (en) * 2001-03-23 2003-01-02 Fuji Photo Film Co., Ltd. Waste water inspecting agent and waste water inspecting apparatus using the same
CA2466821A1 (en) * 2001-11-21 2003-06-05 Applera Corporation Digital assay
JP4274948B2 (ja) * 2002-03-13 2009-06-10 シンジェンタ・パティシペーションズ・アクチェンゲゼルシャフト 核酸検出方法
US20030179381A1 (en) * 2002-03-18 2003-09-25 Fuji Photo Film Co., Ltd. Sensor, color sensor and apparatus for inspection using the same
US20040137468A1 (en) * 2002-08-30 2004-07-15 Brian Warner Solid phase based nucleic acid assays combining high affinity and high specificity
US8394944B2 (en) * 2002-09-20 2013-03-12 Siemens Healthcare Diagnostics Inc. Dual-purpose primers and probes for providing enhanced hybridization assays by disruption of secondary structure formation
US20040235005A1 (en) * 2002-10-23 2004-11-25 Ernest Friedlander Methods and composition for detecting targets
JP4294946B2 (ja) * 2002-12-13 2009-07-15 富士フイルム株式会社 標的検出装置及び標的検出方法並びに標的検出用基材
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US7076127B2 (en) * 2003-01-14 2006-07-11 Fuji Photo Film Co., Ltd. Optical switch and safety apparatus using the same
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US20050239089A1 (en) * 2003-06-06 2005-10-27 Johnson Martin D Mobility cassettes
US20060008823A1 (en) * 2004-05-12 2006-01-12 Kemp Jennifer T DNA profiling and SNP detection utilizing microarrays
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
US20070020699A1 (en) * 2005-07-19 2007-01-25 Idexx Laboratories, Inc. Lateral flow assay and device using magnetic particles
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
JP2012506705A (ja) 2008-10-27 2012-03-22 キアジェン ゲイサーズバーグ インコーポレイテッド 迅速に結果を得るハイブリッドキャプチャー法による分析およびシステム
WO2010088292A1 (en) * 2009-01-28 2010-08-05 Qiagen Gaithersburg, Inc. Sequence-specific large volume sample preparation method and assay
CA2760542A1 (en) 2009-05-01 2010-11-04 Qiagen Gaithersburg, Inc. A non-target amplification method for detection of rna splice-forms in a sample
AU2010291990B2 (en) 2009-09-14 2016-05-05 Qiagen Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
CN102822189B (zh) * 2010-01-29 2016-06-29 奇亚根盖瑟斯堡股份有限公司 用于核酸的序列特异性纯化和多重复合体分析(multiplex analysis)的方法和组合物
BR112012018545A2 (pt) 2010-01-29 2016-05-03 Qiagen Gaithersburg Inc método de determinação e confirmação da presença de um hpv em uma amostra
CA2799200A1 (en) 2010-05-19 2011-11-24 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
US9885092B2 (en) 2011-02-24 2018-02-06 Qiagen Gaithersburg Inc. Materials and methods for detection of HPV nucleic acids
US9447458B2 (en) * 2011-11-16 2016-09-20 Canon U.S. Life Sciences, Inc. Detection of neighboring variants

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
US5242794A (en) * 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4883750A (en) * 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4925785A (en) * 1986-03-07 1990-05-15 Biotechnica Diagnostics, Inc. Nucleic acid hybridization assays
US5312910A (en) * 1987-05-26 1994-05-17 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase
US4988617A (en) * 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
CA1341584C (en) * 1988-04-06 2008-11-18 Bruce Wallace Method of amplifying and detecting nucleic acid sequences
WO1989009835A1 (en) * 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Ligase-based amplification method
US5241070A (en) * 1988-09-26 1993-08-31 Ciba Corning Diagnostics Corp. Nucleophilic polysubstituted aryl acridinium esters and uses thereof
DE69133629D1 (de) * 1990-05-03 2010-04-15 Cornell Res Foundation Inc nssystem zur Bestimmung von genetischen Krankheiten
CA2049879A1 (en) * 1990-08-30 1992-03-01 Keith C. Backman Controlled initial target-dependent production of templates for ligase chain reaction
CA2046713A1 (en) * 1990-10-16 1992-04-17 Richard M. Martinelli Amplification of midivariant dna templates
NZ245242A (en) * 1992-01-17 1994-12-22 Pioneer Hi Bred Int Identifying variations between organisms by detecting polymorphisms in single sequence repeats
WO1993020236A1 (en) * 1992-04-03 1993-10-14 Applied Biosystems, Inc. Probe composition and method
WO1994008047A1 (en) * 1992-09-25 1994-04-14 Abbott Laboratories Ligase chain reaction method for detecting small mutations

Also Published As

Publication number Publication date
BR9507343A (pt) 1997-09-16
AU684915B2 (en) 1998-01-08
EP0754241A1 (de) 1997-01-22
US5800994A (en) 1998-09-01
DE69506393T2 (de) 1999-07-01
AU1958595A (en) 1995-10-23
ATE174066T1 (de) 1998-12-15
CA2186465A1 (en) 1995-10-12
EP0754241B1 (de) 1998-12-02
WO1995027078A1 (en) 1995-10-12
JP3738910B2 (ja) 2006-01-25
PL316611A1 (en) 1997-01-20
JPH09510878A (ja) 1997-11-04

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