DE69605669T2 - Bestimmung der Oligonucleotid-Länge unter Verwendung von spaltbaren Primern - Google Patents

Bestimmung der Oligonucleotid-Länge unter Verwendung von spaltbaren Primern

Info

Publication number
DE69605669T2
DE69605669T2 DE69605669T DE69605669T DE69605669T2 DE 69605669 T2 DE69605669 T2 DE 69605669T2 DE 69605669 T DE69605669 T DE 69605669T DE 69605669 T DE69605669 T DE 69605669T DE 69605669 T2 DE69605669 T2 DE 69605669T2
Authority
DE
Germany
Prior art keywords
determination
oligonucleotide length
cleavable primers
cleavable
primers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69605669T
Other languages
English (en)
Other versions
DE69605669D1 (de
Inventor
Joseph Monforte
Christopher Becker
Thomas Shaler
Daniel Pollart
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SRI International Inc
Original Assignee
SRI International Inc
Stanford Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/445,751 external-priority patent/US5700642A/en
Application filed by SRI International Inc, Stanford Research Institute filed Critical SRI International Inc
Application granted granted Critical
Publication of DE69605669D1 publication Critical patent/DE69605669D1/de
Publication of DE69605669T2 publication Critical patent/DE69605669T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6872—Methods for sequencing involving mass spectrometry
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/26—Mass spectrometers or separator tubes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction
DE69605669T 1995-05-22 1996-04-30 Bestimmung der Oligonucleotid-Länge unter Verwendung von spaltbaren Primern Expired - Lifetime DE69605669T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/445,751 US5700642A (en) 1995-05-22 1995-05-22 Oligonucleotide sizing using immobilized cleavable primers
US08/639,363 US5830655A (en) 1995-05-22 1996-04-26 Oligonucleotide sizing using cleavable primers
PCT/US1996/006116 WO1996037630A1 (en) 1995-05-22 1996-04-30 Oligonucleotide sizing using cleavable primers

Publications (2)

Publication Number Publication Date
DE69605669D1 DE69605669D1 (de) 2000-01-20
DE69605669T2 true DE69605669T2 (de) 2000-07-27

Family

ID=27034396

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69605669T Expired - Lifetime DE69605669T2 (de) 1995-05-22 1996-04-30 Bestimmung der Oligonucleotid-Länge unter Verwendung von spaltbaren Primern

Country Status (9)

Country Link
US (3) US5830655A (de)
EP (1) EP0828855B1 (de)
JP (1) JP3437184B2 (de)
KR (1) KR19990021950A (de)
CN (1) CN1191575A (de)
AU (1) AU695705B2 (de)
CA (1) CA2220418C (de)
DE (1) DE69605669T2 (de)
WO (1) WO1996037630A1 (de)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10142643A1 (de) * 2001-08-31 2003-04-24 Clondiag Chip Tech Gmbh Detektion von Wechselwirkungen auf Sonden-Arrays
DE102005060925A1 (de) * 2005-12-14 2007-06-21 Universität Leipzig Verfahren zur Detektion von DNA-Basensequenzveränderungen
DE102006007778B3 (de) * 2006-02-20 2007-11-08 Biotype Ag Verfahren zur Analyse von Multiplex-Gemischen bestehend aus Nukleinsäure-Amplifikationsprodukten mit überlappenden Detektionsbereichen

Families Citing this family (229)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6589726B1 (en) 1991-09-04 2003-07-08 Metrigen, Inc. Method and apparatus for in situ synthesis on a solid support
US5795714A (en) 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
CA2153387A1 (en) 1993-01-07 1994-07-21 Hubert Koester Dna sequencing by mass spectrometry
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US6194144B1 (en) 1993-01-07 2001-02-27 Sequenom, Inc. DNA sequencing by mass spectrometry
US6428955B1 (en) 1995-03-17 2002-08-06 Sequenom, Inc. DNA diagnostics based on mass spectrometry
US7803529B1 (en) 1995-04-11 2010-09-28 Sequenom, Inc. Solid phase sequencing of biopolymers
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US6146854A (en) * 1995-08-31 2000-11-14 Sequenom, Inc. Filtration processes, kits and devices for isolating plasmids
AU2069597A (en) 1996-03-04 1997-09-22 Genetrace Systems, Inc. Methods of screening nucleic acids using mass spectrometry
US6022688A (en) 1996-05-13 2000-02-08 Sequenom, Inc. Method for dissociating biotin complexes
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
US5777324A (en) 1996-09-19 1998-07-07 Sequenom, Inc. Method and apparatus for maldi analysis
US5965363A (en) * 1996-09-19 1999-10-12 Genetrace Systems Inc. Methods of preparing nucleic acids for mass spectrometric analysis
US5885775A (en) * 1996-10-04 1999-03-23 Perseptive Biosystems, Inc. Methods for determining sequences information in polynucleotides using mass spectrometry
US6140053A (en) * 1996-11-06 2000-10-31 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
US6133436A (en) * 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US6024925A (en) 1997-01-23 2000-02-15 Sequenom, Inc. Systems and methods for preparing low volume analyte array elements
US7285422B1 (en) * 1997-01-23 2007-10-23 Sequenom, Inc. Systems and methods for preparing and analyzing low volume analyte array elements
DE69727489T2 (de) 1996-11-06 2004-11-25 Sequenom, Inc., San Diego Verfahren zur massenspektrometrie
DE69735445T2 (de) 1996-12-10 2006-08-10 Sequenom, Inc., San Diego Abspaltbare, nicht-flüchtige moleküle zur massenmarkierung
DE19710166C1 (de) * 1997-03-12 1998-12-10 Bruker Franzen Analytik Gmbh Zwei-Schritt-Verfahren der DNA-Amplifikation für MALDI-TOF-Messungen
US6043070A (en) * 1997-08-29 2000-03-28 The Perkin-Elmer Corporation Phosphoramidate-phosphodiester oligonucleotide chimera as primers
US6207370B1 (en) 1997-09-02 2001-03-27 Sequenom, Inc. Diagnostics based on mass spectrometric detection of translated target polypeptides
US6268131B1 (en) 1997-12-15 2001-07-31 Sequenom, Inc. Mass spectrometric methods for sequencing nucleic acids
US6723564B2 (en) 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
US6104028A (en) * 1998-05-29 2000-08-15 Genetrace Systems Inc. Volatile matrices for matrix-assisted laser desorption/ionization mass spectrometry
DE19824280B4 (de) * 1998-05-29 2004-08-19 Bruker Daltonik Gmbh Mutationsanalyse mittels Massenspektrometrie
US6566059B1 (en) * 1998-10-01 2003-05-20 Variagenics, Inc. Method for analyzing polynucleotides
US6500650B1 (en) 1998-10-01 2002-12-31 Variagenics, Inc. Method for identifying polymorphisms
US6440705B1 (en) 1998-10-01 2002-08-27 Vincent P. Stanton, Jr. Method for analyzing polynucleotides
US6855500B2 (en) 1998-10-01 2005-02-15 Sequenom, Inc. Fluorescence-based genotyping
US6777188B2 (en) 1998-10-01 2004-08-17 Variagenics, Inc. Genotyping by mass spectrometric analysis of allelic fragments
US6610492B1 (en) 1998-10-01 2003-08-26 Variagenics, Inc. Base-modified nucleotides and cleavage of polynucleotides incorporating them
US6458945B1 (en) 1998-10-01 2002-10-01 Variagenics, Inc. Method for analyzing polynucleotides
US6994998B1 (en) 1998-10-01 2006-02-07 Sequenom, Inc. Base-modified nucleotides and their use for polymorphism detection
EP1042512A2 (de) * 1998-10-26 2000-10-11 Yale University Allel häufigkeitsunterschiede - verfahren zur phenotypklonierung
AU1739500A (en) * 1998-11-24 2000-06-13 Johns Hopkins University, The Genotyping by mass spectrometric analysis of short dna fragments
AU3623800A (en) * 1999-03-08 2000-09-28 Protogene Laboratories, Inc. Methods and compositions for economically synthesizing and assembling long dna sequences
US6844151B1 (en) * 1999-09-29 2005-01-18 Oligos Etc. Inc. Methods for production of arrays with modified oligonucleotide and polynucleotide compositions
WO2001027327A2 (en) 1999-10-08 2001-04-19 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
JP2001245698A (ja) * 1999-11-22 2001-09-11 Xiao Bing Wang 核酸検出法
US7186813B1 (en) 2000-01-11 2007-03-06 Nanogen Recognomics Gmbh Biomolecules having multiple attachment moieties for binding to a substrate surface
CA2398107C (en) 2000-01-28 2013-11-19 Althea Technologies, Inc. Methods for analysis of gene expression
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
EP1990428B1 (de) * 2000-02-07 2010-12-22 Illumina, Inc. Nukleinsäuredetektionsverfahren mit universellem Priming
US6911535B2 (en) * 2000-03-22 2005-06-28 Solvlink Biosciences Biomolecule/polymer conjugates
DE10015797B4 (de) * 2000-03-26 2006-02-02 Bruker Daltonik Gmbh Multiplex-Analyse von DNA-Gemischen mittels photolytisch ablesbarer DNA-Chips
DE10021204A1 (de) * 2000-04-25 2001-11-08 Epigenomics Ag Verfahren zur hochparallelen Analyse von Polymorphismen
DE10123183A1 (de) * 2000-05-18 2001-11-22 Becton Dickinson Co Modifizierte Amplifikationsprimer und ihre Verwendung
US6824980B2 (en) * 2000-06-08 2004-11-30 Xiao Bing Wang Isometric primer extension method and kit for detection and quantification of specific nucleic acid
EP1679381A1 (de) * 2000-06-08 2006-07-12 Xiao Bing Wang Verfahren zur isomeren Primerverlängerung und Reagenziensatz zum Nachweis und zur Quantifizierung von spezifischen Nukleinsäuren
ATE423221T1 (de) 2000-06-13 2009-03-15 Univ Boston Verwendung von mass-matched nukleotide in der analyse von oligonukleotidmischungen sowie in der hoch-multiplexen nukleinsäuresequenzierung
WO2002000938A2 (en) * 2000-06-26 2002-01-03 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US6994963B1 (en) * 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
US7129229B2 (en) 2000-08-11 2006-10-31 Nanogen Recognomics Gmbh Hydrazide building blocks and hydrazide modified biomolecules
AU2001280153A1 (en) * 2000-08-25 2002-03-04 Fujirebio Inc. Method of protecting personal information
DE10046184A1 (de) * 2000-09-18 2002-04-04 November Ag Molekulare Medizin Verfahren zum Nachweis mindestens einer Nukleinsäuresequenz
US20020142483A1 (en) 2000-10-30 2002-10-03 Sequenom, Inc. Method and apparatus for delivery of submicroliter volumes onto a substrate
WO2002048402A2 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US20020168652A1 (en) * 2000-12-22 2002-11-14 Werner Martina Elisabeth Surface assembly for immobilizing DNA capture probes and bead-based assay including optical bio-discs and methods relating thereto
CN1500144A (zh) * 2001-02-06 2004-05-26 �����﹤����ʽ���� 扩增的核酸及其固定化制品
DE10108453B4 (de) * 2001-02-22 2005-10-20 Bruker Daltonik Gmbh Massenspektrometrische Mutationsanalyse mit photolytisch spaltbaren Primern
US20040121314A1 (en) * 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in containers
US20040121313A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in organs for transplantation
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
WO2004060278A2 (en) 2002-12-06 2004-07-22 Isis Pharmaceuticals, Inc. Methods for rapid identification of pathogens in humans and animals
US7718354B2 (en) * 2001-03-02 2010-05-18 Ibis Biosciences, Inc. Methods for rapid identification of pathogens in humans and animals
US20040121310A1 (en) * 2002-12-18 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in forensic studies
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
US7094536B2 (en) * 2001-03-09 2006-08-22 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences
ZA200210369B (en) * 2001-03-09 2004-07-08 Nugen Technologies Inc Methods and compositions for amplification or RNA sequences.
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
US8073627B2 (en) 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
JP2003043037A (ja) * 2001-07-27 2003-02-13 Inst Of Physical & Chemical Res ハイブリダイゼーション用基板、この基板の製造方法及び使用方法
US20050147984A1 (en) * 2001-08-31 2005-07-07 Clondiag Chip Technologies Gmbh Interaction detection on several probe arrays
IL146270A0 (en) * 2001-11-01 2002-07-25 Yeda Res & Dev Pre-existing nucleic acids covalently attached to a metal surface or a metal cluster, intermediates thereof and methods of using same
AU2002366098A1 (en) * 2001-11-19 2003-06-10 Parallele Bioscience Inc Multiplex pcr
US7223538B2 (en) * 2001-12-14 2007-05-29 Ge Healthcare Bio-Sciences Ab Post-synthesis labeling of nucleic acids, assays using nucleic acids that are labeled post-synthetically, single nucleotide polymorphism detection, and associated compounds and microarrays
DE10164219A1 (de) * 2001-12-31 2003-07-17 Reinald Repp Verfahren zur Detektion von Nukleinsäuren unter Verwendung festphasengebundener Primer und Abspaltung der Produkte einer zyklischen Amplifikationsreaktion (Trap Release Primer Amplification (TRAmp)
ATE358185T1 (de) * 2002-02-01 2007-04-15 Bruker Daltonik Gmbh Mutationsanalyse mittels pcr und massenspektrometrie
JP2003299489A (ja) * 2002-02-08 2003-10-21 Mitsubishi Chemicals Corp 核酸構築物
US7176025B2 (en) * 2002-03-11 2007-02-13 Nugen Technologies, Inc. Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination
US6723546B2 (en) * 2002-03-26 2004-04-20 New England Biolabs, Inc. Method for cloning and expression of BsaI restriction endonuclease and BsaI methylase in E. coli
ATE378424T1 (de) * 2002-08-12 2007-11-15 Gnothis Holding Sa Nachweis von einzelnukleotidpolymorphismen durch einzelmolekülanalyse
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US20040185444A1 (en) * 2003-03-18 2004-09-23 Nicole Boehringer-Wyss System for predicting susceptibility of human oropharyngeal squamous cell carcinoma to radiotherapy
WO2004092418A2 (en) * 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US8046171B2 (en) 2003-04-18 2011-10-25 Ibis Biosciences, Inc. Methods and apparatus for genetic evaluation
US8057993B2 (en) 2003-04-26 2011-11-15 Ibis Biosciences, Inc. Methods for identification of coronaviruses
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
EP1493824A1 (de) * 2003-07-02 2005-01-05 Consortium National de Recherche en Genomique (CNRG) Verfahren zur detektion von Mutationen in DNA
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US20120122096A1 (en) 2003-09-11 2012-05-17 Rangarajan Sampath Compositions for use in identification of bacteria
WO2005029041A2 (en) * 2003-09-19 2005-03-31 Applera Corporation High density sequence detection methods and apparatus
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US8163895B2 (en) 2003-12-05 2012-04-24 Ibis Biosciences, Inc. Compositions for use in identification of orthopoxviruses
EP1725680B1 (de) * 2004-02-04 2009-12-23 QIAGEN North American Holdings, Inc. Zusammensetzungen auf dutp-basis zurverringerung der primeraggregatbildung während der nukleinsäureamplifikation
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
EP2248911A1 (de) * 2004-02-19 2010-11-10 Helicos Biosciences Corporation Verfahren und Zusammensetzungen zur Analyse von Polynukleotidsequenzen
US8119336B2 (en) 2004-03-03 2012-02-21 Ibis Biosciences, Inc. Compositions for use in identification of alphaviruses
EP2458619B1 (de) 2004-05-24 2017-08-02 Ibis Biosciences, Inc. Massenspektrometrie mit selektivem Ionfilter durch digitale Schwelle
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
WO2006094238A2 (en) 2005-03-03 2006-09-08 Isis Pharmaceuticals, Inc. Compositions for use in identification of adventitious viruses
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
US20060286572A1 (en) * 2005-05-16 2006-12-21 Kohne David E Method for producing chemically synthesized and in vitro enzymatically synthesized nucleic acid oligomers
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
CA2616281C (en) 2005-07-21 2014-04-22 Isis Pharmaceuticals, Inc. Methods for rapid identification and quantitation of mitochondrial dna variants
US8062850B2 (en) 2005-07-25 2011-11-22 Alere San Diego, Inc. Methods for multiplexing recombinase polymerase amplification
US7749701B2 (en) * 2005-08-11 2010-07-06 Agilent Technologies, Inc. Controlling use of oligonucleotide sequences released from arrays
CA2621267A1 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
US7625702B2 (en) * 2005-12-20 2009-12-01 International Business Machines Corporation Helical wrapping of single-walled carbon nanotubes by genomic DNA
US20070172839A1 (en) * 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
US8088582B2 (en) 2006-04-06 2012-01-03 Ibis Biosciences, Inc. Compositions for the use in identification of fungi
CA2653671A1 (en) * 2006-05-30 2007-12-06 Synergenz Bioscience Limited Methods and compositions for assessment of pulmonary function and disorders
DE602007013223D1 (de) * 2006-06-01 2011-04-28 Trilink Biotechnologies San Diego Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
AU2007353877B2 (en) 2006-09-14 2012-07-19 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
WO2008045380A2 (en) * 2006-10-04 2008-04-17 Codon Devices, Inc. Nucleic acid libraries and their design and assembly
US8871471B2 (en) 2007-02-23 2014-10-28 Ibis Biosciences, Inc. Methods for rapid forensic DNA analysis
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
US20090036660A1 (en) * 2007-07-31 2009-02-05 Joel Myerson Methods and compositions for generating mixtures of nucleic acid molecules
US20090180931A1 (en) 2007-09-17 2009-07-16 Sequenom, Inc. Integrated robotic sample transfer device
US20090075840A1 (en) * 2007-09-18 2009-03-19 Joel Myerson Methods And Compositions For Generating Mixtures Of Nucleic Acid Molecules
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
GB2470672B (en) 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
US8808986B2 (en) 2008-08-27 2014-08-19 Gen9, Inc. Methods and devices for high fidelity polynucleotide synthesis
WO2010033599A2 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Mixing cartridges, mixing stations, and related kits, systems, and methods
US8148163B2 (en) 2008-09-16 2012-04-03 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
EP2344893B1 (de) 2008-09-16 2014-10-15 Ibis Biosciences, Inc. Mikroplatten-handhabungssysteme und verfahren
EP2379748A4 (de) * 2008-12-23 2012-08-29 Illumina Inc Multibase-freisetzung für lange auslesevorgänge bei squenzierungen mittels syntheseprotokollen
EP2396803A4 (de) 2009-02-12 2016-10-26 Ibis Biosciences Inc Ionisationssondenanordnungen
US9347092B2 (en) 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
WO2010104798A1 (en) 2009-03-08 2010-09-16 Ibis Biosciences, Inc. Bioagent detection methods
WO2010107946A2 (en) * 2009-03-18 2010-09-23 Sequenom, Inc. Use of thermostable endonucleases for generating reporter molecules
WO2010114842A1 (en) 2009-03-30 2010-10-07 Ibis Biosciences, Inc. Bioagent detection systems, devices, and methods
WO2011008971A1 (en) 2009-07-17 2011-01-20 Ibis Biosciences, Inc. Lift and mount apparatus
US9194877B2 (en) 2009-07-17 2015-11-24 Ibis Biosciences, Inc. Systems for bioagent indentification
US9416409B2 (en) 2009-07-31 2016-08-16 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
EP2462244B1 (de) 2009-08-06 2016-07-20 Ibis Biosciences, Inc. Basiszusammensetzungen ohne massebestimmung zur nukleinsäureerkennung
EP2957641B1 (de) 2009-10-15 2017-05-17 Ibis Biosciences, Inc. Multiple-displacement-amplifikation
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
EP2507393A4 (de) 2009-11-30 2013-05-01 Caris Life Sciences Luxembourg Holdings Verfahren und systeme zur isolation, speicherung und analyse von vesikeln
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
JP5808349B2 (ja) 2010-03-01 2015-11-10 カリス ライフ サイエンシズ スウィッツァーランド ホールディングスゲーエムベーハー セラノーシスのためのバイオマーカー
US9758840B2 (en) 2010-03-14 2017-09-12 Ibis Biosciences, Inc. Parasite detection via endosymbiont detection
KR20130043104A (ko) 2010-04-06 2013-04-29 카리스 라이프 사이언스 룩셈부르크 홀딩스 질병용 순환 생물학적 지표들
WO2011143556A1 (en) 2010-05-13 2011-11-17 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
WO2011150168A1 (en) 2010-05-28 2011-12-01 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
US8865404B2 (en) 2010-11-05 2014-10-21 President And Fellows Of Harvard College Methods for sequencing nucleic acid molecules
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
EP3000883B8 (de) 2010-11-12 2018-02-28 Gen9, Inc. Verfahren und vorrichtungen für nukleinsäuresynthese
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
US8709722B2 (en) * 2011-08-24 2014-04-29 Agency For Science, Technology And Research Methods for detecting DNA-binding proteins
DK3594340T3 (da) 2011-08-26 2021-09-20 Gen9 Inc Sammensætninger og fremgangsmåder til samling med høj nøjagtighed af nukleinsyrer
US10501791B2 (en) 2011-10-14 2019-12-10 President And Fellows Of Harvard College Sequencing by structure assembly
US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
PE20142451A1 (es) 2012-02-27 2015-02-04 Amunix Operating Inc Composiciones de conjugados de xten y metodos para realizar las mismas
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
KR101756975B1 (ko) * 2012-04-19 2017-07-12 주식회사 씨젠 Ｐｔｏ 절단 및 연장-의존적 시그널링 올리고뉴클레오타이드 절단을 이용한 타겟 핵산서열의 검출
EP3543350B1 (de) 2012-04-24 2021-11-10 Gen9, Inc. Verfahren zum sortieren von nukleinsäuren und zur multiplexierten, vorbereitenden in-vitro-klonung
US8809435B2 (en) 2012-05-16 2014-08-19 The United States Of America As Represented By The Secretary Of The Army Process enhancement via stimuli responsive particle surfaces
CN108587867B (zh) * 2012-05-25 2021-12-17 北卡罗来纳-查佩尔山大学 微流体装置、用于试剂的固体支持体和相关方法
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
IL236303B (en) 2012-06-25 2022-07-01 Gen9 Inc Methods for high-throughput nucleic acid assembly and sequencing
GB201215435D0 (en) * 2012-08-30 2012-10-17 Geneseque As Sequencing method
US9476089B2 (en) 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
KR101863943B1 (ko) * 2013-07-15 2018-06-01 주식회사 씨젠 Ｐｔｏ 절단 및 연장-의존적 고정화 올리고뉴클레오타이드 혼성화를 이용한 타겟 핵산 서열의 검출
DK3030682T3 (da) 2013-08-05 2020-09-14 Twist Bioscience Corp De novo synthesized gene libraries
EP3058105B1 (de) * 2013-10-18 2019-05-22 Seegene, Inc. Nachweis einer zielnukleinsäuresequenz in fester phase durch pto-spaltung und extension mit einem hcto-test
WO2016040901A1 (en) * 2014-09-12 2016-03-17 The Board Of Trustees Of The Leland Stanford Junior University Identification and use of circulating nucleic acids
CN105420225A (zh) * 2014-09-17 2016-03-23 苏州旷世骏弛生物科技有限公司 基于硫代修饰的碘酒裂解型基因克隆方法
US20160083724A1 (en) * 2014-09-24 2016-03-24 University Of Southern California Methods for sample preparation
WO2016081585A1 (en) * 2014-11-20 2016-05-26 Ampliwise Inc. Compositions and methods for nucleic acid amplification
EP3034619B1 (de) * 2014-12-16 2018-10-17 microBIOMix GmbH Modifizierte Oligonukleotide und Verfahren zum Einschleusen von Primern oder Kontrollmolekülen oder Sonden in geschlossene PCR-Systeme
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016126987A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US20170016051A1 (en) * 2015-07-15 2017-01-19 Orizhan Bioscience Limited DNA Methylation Detection
WO2017065854A2 (en) 2015-07-22 2017-04-20 The University Of North Carolina At Chapel Hill Fluidic devices with bead well geometries with spatially separated bead retention and signal detection segments and related methods
WO2017049231A1 (en) 2015-09-18 2017-03-23 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
US11512347B2 (en) 2015-09-22 2022-11-29 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
CA3006867A1 (en) 2015-12-01 2017-06-08 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
KR101961642B1 (ko) 2016-04-25 2019-03-25 (주)진매트릭스 절단된 상보적인 태그 절편을 이용한 표적 핵산 서열 검출 방법 및 그 조성물
EP3500672A4 (de) 2016-08-22 2020-05-20 Twist Bioscience Corporation De-novo-synthetisierte nukleinsäure-bibliotheken
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
SG10201709799SA (en) * 2016-11-29 2018-06-28 Helios Bioelectronics Inc Method for quantitatively profiling nucleic acids
EP3554514A4 (de) 2016-12-16 2020-08-05 Twist Bioscience Corporation Variante bibliotheken der immunologischen synapse und synthese davon
EP3555290B1 (de) 2016-12-19 2022-11-02 Bio-Rad Laboratories, Inc. Markierte dna mit erhaltener tröpfchenmarkierungskontinuität
EP3586255A4 (de) 2017-02-22 2021-03-31 Twist Bioscience Corporation Nukleinsäurebasierte datenspeicherung
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
AU2018284227A1 (en) 2017-06-12 2020-01-30 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11667952B2 (en) 2017-08-24 2023-06-06 Takara Bio Usa, Inc. Methods of producing nucleic acids using oligonucleotides modified by a stimulus
KR20200047706A (ko) 2017-09-11 2020-05-07 트위스트 바이오사이언스 코포레이션 Gpcr 결합 단백질 및 이의 합성 방법
SG11202003574TA (en) 2017-10-20 2020-05-28 Twist Bioscience Corp Heated nanowells for polynucleotide synthesis
CA3088911A1 (en) 2018-01-04 2019-07-11 Twist Bioscience Corporation Dna-based digital information storage
WO2019222706A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
WO2020020608A1 (en) 2018-07-23 2020-01-30 Dna Script Massively parallel enzymatic synthesis of nucleic acid strands
EP3906317A1 (de) 2019-01-03 2021-11-10 DNA Script Eintopfsynthese von sätzen von oligonukleotiden
WO2020176680A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
US11492727B2 (en) 2019-02-26 2022-11-08 Twist Bioscience Corporation Variant nucleic acid libraries for GLP1 receptor
CN114729342A (zh) 2019-06-21 2022-07-08 特韦斯特生物科学公司 基于条形码的核酸序列装配
AU2020319829A1 (en) 2019-07-30 2022-02-24 Dna Script Template-free enzymatic synthesis of polynucleotides using poly(A) and poly(U) polymerases
CN114430778A (zh) 2019-08-01 2022-05-03 Dna斯克瑞普特公司 增加多核苷酸的无模板酶促合成中长序列的产量
EP4034669A1 (de) 2019-09-23 2022-08-03 DNA Script Erhöhung der ausbeute von langen sequenzen bei der schablonenfreien enzymatischen synthese von polynukleotiden
US11873484B2 (en) 2019-11-27 2024-01-16 Microsoft Technology Licensing, Llc Oligonucleotide assembly using electrically controlled hybridization
US20210205775A1 (en) * 2020-01-06 2021-07-08 Microsoft Technology Licensing, Llc Solid-phase polymer synthesis on reusable substrates
EP4110940B1 (de) 2020-02-25 2024-03-27 DNA Script Verfahren und vorrichtung zur enzymatischen synthese von polynukleotiden
WO2021213903A1 (en) 2020-04-20 2021-10-28 Dna Script Terminal deoxynucleotidyl transferase variants and uses thereof
JP2023531620A (ja) 2020-06-16 2023-07-25 ディーエヌエー スクリプト 酵素的ポリヌクレオチド合成のためのシステム、装置及びキット
WO2022013094A1 (en) 2020-07-15 2022-01-20 Dna Script Massively parallel enzymatic synthesis of polynucleotides
WO2022063835A1 (en) 2020-09-22 2022-03-31 Dna Script Stabilized n-terminally truncated terminal deoxynucleotidyl transferase variants and uses thereof
CN112941147A (zh) * 2021-03-02 2021-06-11 深圳市睿法生物科技有限公司 一种高保真靶标基因建库方法及其试剂盒
AU2022249826A1 (en) 2021-04-02 2023-09-21 Dna Script Methods and kits for enzymatic synthesis of g4-prone polynucleotides
WO2023035003A1 (en) 2021-09-03 2023-03-09 Elegen Corp. Multi-way bead-sorting devices, systems, and methods of use thereof using pressure sources
WO2023170266A1 (en) 2022-03-11 2023-09-14 Dna Script Automation station for enzymatic polynucleotide synthesis
WO2023170259A1 (en) 2022-03-11 2023-09-14 Dna Script Modular accessory rack
WO2023170258A1 (en) 2022-03-11 2023-09-14 Dna Script Apparatus for enzymatic synthesis of a plurality of polynucleotides comprising a condensation trap
WO2023170286A2 (en) 2022-03-11 2023-09-14 Dna Script Alignment post and secure mechanism for enzymatic polynucleotide synthesis

Family Cites Families (102)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4139346A (en) 1977-11-28 1979-02-13 Enzo Bio Chem Incorporated Nucleic acid and protein binding paper
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US5198540A (en) 1982-10-28 1993-03-30 Hubert Koster Process for the preparation of oligonucleotides in solution
US5059654A (en) 1983-02-14 1991-10-22 Cuno Inc. Affinity matrices of modified polysaccharide supports
US4515781A (en) 1983-02-23 1985-05-07 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services 2',5'-Riboadenylate-morpholinoadenylate nucleotides
JPS59161398A (ja) * 1983-03-02 1984-09-12 Mitsui Toatsu Chem Inc デオキシリボ核酸の切断処理方法及び装置
GB8311018D0 (en) 1983-04-22 1983-05-25 Amersham Int Plc Detecting mutations in dna
DE3329892A1 (de) 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur herstellung von oligonucleotiden
US4582789A (en) 1984-03-21 1986-04-15 Cetus Corporation Process for labeling nucleic acids using psoralen derivatives
US4729947A (en) 1984-03-29 1988-03-08 The Board Of Regents Of The University Of Nebraska DNA sequencing
US4957858A (en) 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
US4683194A (en) 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4849077A (en) 1984-08-06 1989-07-18 Akademie Der Wissenschaften Der Ddr Process for solid phase-sequencing of nucleic acid fragments
US5118605A (en) 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US5430136A (en) * 1984-10-16 1995-07-04 Chiron Corporation Oligonucleotides having selectably cleavable and/or abasic sites
US4775619A (en) * 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4604363A (en) 1984-10-18 1986-08-05 Analytical Bio-Chemistry Laboratories Inc. Automatic evaporator system
US5221518A (en) 1984-12-14 1993-06-22 Mills Randell L DNA sequencing apparatus
US5064754A (en) 1984-12-14 1991-11-12 Mills Randell L Genomic sequencing method
US4818681A (en) * 1985-02-22 1989-04-04 Molecular Diagnostics, Inc. Fast and specific immobilization of nucleic acids to solid supports
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
GB8515276D0 (en) * 1985-06-17 1985-07-17 Amersham Int Plc Nucleic acid sequencing
US4806546A (en) 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on derivatized nylon supports
US4998617A (en) 1986-09-15 1991-03-12 Laura Lupton Inc Facial cosmetic liquid make up kit
US5175209A (en) 1987-01-06 1992-12-29 Baylor College Of Medicine Porous wafer for segmented synthesis of biopolymers
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US5149625A (en) 1987-08-11 1992-09-22 President And Fellows Of Harvard College Multiplex analysis of DNA
US5403711A (en) 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
US5670381A (en) 1988-01-29 1997-09-23 Abbott Laboratories Devices for performing ion-capture binding assays
DE3809504C1 (de) 1988-03-22 1989-09-21 Bruker - Franzen Analytik Gmbh, 2800 Bremen, De
SE8801070D0 (sv) * 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
US5266684A (en) 1988-05-02 1993-11-30 The Reagents Of The University Of California Peptide mixtures
US5003059A (en) * 1988-06-20 1991-03-26 Genomyx, Inc. Determining DNA sequences by mass spectrometry
FR2636738B1 (fr) * 1988-09-20 1992-12-11 Commissariat Energie Atomique Procede et installation de sequencage de l'adn
DE3930312A1 (de) * 1988-10-24 1990-04-26 Max Planck Gesellschaft Verfahren zur direkten sequenzierung von nukleinsaeureketten
US5512439A (en) 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
US5237016A (en) 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
US4920264A (en) 1989-01-17 1990-04-24 Sri International Method for preparing samples for mass analysis by desorption from a frozen solution
US5459039A (en) * 1989-05-12 1995-10-17 Duke University Methods for mapping genetic mutations
US5242974A (en) 1991-11-22 1993-09-07 Affymax Technologies N.V. Polymer reversal on solid surfaces
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
GB2236186B (en) 1989-08-22 1994-01-05 Finnigan Mat Gmbh Process and device for laser desorption of analyte molecular ions, especially of biomolecules
WO1991011533A1 (en) * 1990-01-26 1991-08-08 E.I. Du Pont De Nemours And Company Method for isolating primer extension products from template-directed dna polymerase reactions
US5288644A (en) * 1990-04-04 1994-02-22 The Rockefeller University Instrument and method for the sequencing of genome
CA2036946C (en) 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
HU218095B (hu) * 1990-05-01 2000-05-28 Amgen Inc. Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban
US5135870A (en) 1990-06-01 1992-08-04 Arizona Board Of Regents Laser ablation/ionizaton and mass spectrometric analysis of massive polymers
US5650489A (en) 1990-07-02 1997-07-22 The Arizona Board Of Regents Random bio-oligomer library, a method of synthesis thereof, and a method of use thereof
TW221493B (de) 1990-07-10 1994-03-01 Cardiovascular Diagnostics Inc
DE4024545A1 (de) 1990-08-02 1992-02-06 Boehringer Mannheim Gmbh Verfahren und vorrichtung zum dosierten zufuehren einer biochemischen analysefluessigkeit auf ein target
DK0502180T3 (da) * 1990-09-21 1997-09-22 Amgen Inc Enzymatisk syntese af oligonukleotider
US5846710A (en) 1990-11-02 1998-12-08 St. Louis University Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension
WO1992013629A1 (en) 1991-01-31 1992-08-20 Wayne State University A method for analyzing an organic sample
US5210412A (en) 1991-01-31 1993-05-11 Wayne State University Method for analyzing an organic sample
US5762876A (en) 1991-03-05 1998-06-09 Molecular Tool, Inc. Automatic genotype determination
US5300774A (en) 1991-04-25 1994-04-05 Applied Biosystems, Inc. Time-of-flight mass spectrometer with an aperture enabling tradeoff of transmission efficiency and resolution
ATE165397T1 (de) * 1991-06-27 1998-05-15 Genelabs Tech Inc Screeningtest für den nachweis von dna-bindenden molekülen
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5605662A (en) 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
IL103674A0 (en) 1991-11-19 1993-04-04 Houston Advanced Res Center Method and apparatus for molecule detection
AU675054B2 (en) 1991-11-22 1997-01-23 Affymetrix, Inc. Combinatorial strategies for polymer synthesis
CA2126748A1 (en) * 1991-12-24 1993-06-25 Stephen Minter Manipulating nucleic acid sequences
ATE173767T1 (de) 1992-04-03 1998-12-15 Perkin Elmer Corp Proben zusammensetzung und verfahren
US5710028A (en) 1992-07-02 1998-01-20 Eyal; Nurit Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
US5503980A (en) 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
US5795714A (en) 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
US5436143A (en) 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
CA2153387A1 (en) 1993-01-07 1994-07-21 Hubert Koester Dna sequencing by mass spectrometry
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US5571669A (en) 1993-01-14 1996-11-05 The University Of Pennsylvania Transcription and nucleic acid sequence determination with short primer DNA/RNA molecules and RNA polymerase
CA2115811A1 (en) 1993-02-17 1994-08-18 Claus Krebber A method for in vivo selection of ligand-binding proteins
EP0612994A3 (de) 1993-02-26 1996-03-06 Ciba Geigy Ag Matrix für die matrix-unterstützte Laserdesorptions-Massenspektroskopie.
JPH08507926A (ja) * 1993-03-19 1996-08-27 シーケノム・インコーポレーテツド エキソヌクレアーゼ分解を介した質量分析法によるｄｎａ配列決定
US5593826A (en) 1993-03-22 1997-01-14 Perkin-Elmer Corporation, Applied Biosystems, Inc. Enzymatic ligation of 3'amino-substituted oligonucleotides
US5376788A (en) 1993-05-26 1994-12-27 University Of Manitoba Apparatus and method for matrix-assisted laser desorption mass spectrometry
GB9315847D0 (en) 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
US5527675A (en) 1993-08-20 1996-06-18 Millipore Corporation Method for degradation and sequencing of polymers which sequentially eliminate terminal residues
WO1995009688A1 (en) 1993-10-04 1995-04-13 Hewlett-Packard Company Sample preparation system and method
US5622829A (en) 1993-12-08 1997-04-22 The Regents Of The University Of California Genetic markers for breast, ovarian, and prostatic cancer
US5538897A (en) 1994-03-14 1996-07-23 University Of Washington Use of mass spectrometry fragmentation patterns of peptides to identify amino acid sequences in databases
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5834189A (en) 1994-07-08 1998-11-10 Visible Genetics Inc. Method for evaluation of polymorphic genetic sequences, and the use thereof in identification of HLA types
US5498545A (en) 1994-07-21 1996-03-12 Vestal; Marvin L. Mass spectrometer system and method for matrix-assisted laser desorption measurements
US5763594A (en) * 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US5512295A (en) 1994-11-10 1996-04-30 The Board Of Trustees Of The Leland Stanford Junior University Synthetic liposomes for enhanced uptake and delivery
US5688642A (en) 1994-12-01 1997-11-18 The United States Of America As Represented By The Secretary Of The Navy Selective attachment of nucleic acid molecules to patterned self-assembled surfaces
US5601982A (en) 1995-02-07 1997-02-11 Sargent; Jeannine P. Method and apparatus for determining the sequence of polynucleotides
US5609907A (en) 1995-02-09 1997-03-11 The Penn State Research Foundation Self-assembled metal colloid monolayers
US5827659A (en) 1995-05-19 1998-10-27 Perseptive Biosystems, Inc. Methods and apparatus for sequencing polymers using mass spectrometry
US5625184A (en) 1995-05-19 1997-04-29 Perseptive Biosystems, Inc. Time-of-flight mass spectrometry analysis of biomolecules
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5700642A (en) 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5571676A (en) 1995-06-07 1996-11-05 Ig Laboratories, Inc. Method for mismatch-directed in vitro DNA sequencing
US5654150A (en) 1995-06-07 1997-08-05 President And Fellows Of Harvard College Method of expression cloning
US5589136A (en) 1995-06-20 1996-12-31 Regents Of The University Of California Silicon-based sleeve devices for chemical reactions
US5716825A (en) 1995-11-01 1998-02-10 Hewlett Packard Company Integrated nucleic acid analysis system for MALDI-TOF MS
US5580434A (en) 1996-02-29 1996-12-03 Hewlett-Packard Company Interface apparatus for capillary electrophoresis to a matrix-assisted-laser-desorption-ionization mass spectrometer
DE19617011C2 (de) 1996-04-27 2000-11-02 Bruker Daltonik Gmbh Matrixkomponentengemisch für die matrixunterstützte Laserdesorption und Ionisierung sowie Verfahren zur Zubereitung eines Matrixkomponentengemisches
DE19628178C1 (de) 1996-07-12 1997-09-18 Bruker Franzen Analytik Gmbh Verfahren zum Beladen von Probenträgern für Massenspektrometer
US5777324A (en) 1996-09-19 1998-07-07 Sequenom, Inc. Method and apparatus for maldi analysis

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10142643A1 (de) * 2001-08-31 2003-04-24 Clondiag Chip Tech Gmbh Detektion von Wechselwirkungen auf Sonden-Arrays
DE102005060925A1 (de) * 2005-12-14 2007-06-21 Universität Leipzig Verfahren zur Detektion von DNA-Basensequenzveränderungen
DE102005060925B4 (de) * 2005-12-14 2010-04-01 Universität Leipzig Verfahren zur Detektion von DNA-Basensequenzveränderungen
DE102006007778B3 (de) * 2006-02-20 2007-11-08 Biotype Ag Verfahren zur Analyse von Multiplex-Gemischen bestehend aus Nukleinsäure-Amplifikationsprodukten mit überlappenden Detektionsbereichen

Also Published As

Publication number Publication date
CN1191575A (zh) 1998-08-26
JPH11505127A (ja) 1999-05-18
US5830655A (en) 1998-11-03
EP0828855A1 (de) 1998-03-18
AU5635296A (en) 1996-12-11
KR19990021950A (ko) 1999-03-25
CA2220418A1 (en) 1996-11-28
US6949633B1 (en) 2005-09-27
EP0828855B1 (de) 1999-12-15
AU695705B2 (en) 1998-08-20
CA2220418C (en) 2003-06-03
WO1996037630A1 (en) 1996-11-28
JP3437184B2 (ja) 2003-08-18
US20060040282A1 (en) 2006-02-23
DE69605669D1 (de) 2000-01-20

Similar Documents

Publication Publication Date Title
DE69605669D1 (de) Bestimmung der Oligonucleotid-Länge unter Verwendung von spaltbaren Primern
ATE364705T1 (de) Verwendung von glyphosat-resistente maislinien
ATE297754T1 (de) Heilung von bruchverletzungen unter verwendung von pthrp analogen
DE69611102D1 (de) Verstärkung der mutagenese von nukleinsäuren
DE69829328D1 (de) Strangverdrängungsamplifikation von RNA
ATE228357T1 (de) Nukleotid-analoge zusammensetzung
DE69727162D1 (de) Herstellung von inulinhaltigen Produkten
DE69628195D1 (de) Verwendung von xyloglucanendotransglycosylase
DE69518743T2 (de) Modifizierung von nukleotidanalogen
ID21013A (id) Deret nukleotida antipeka
DE69801392D1 (de) Herstellung von Methylchlorid
DE69720301D1 (de) Herstellung von Silanen
DE69802139D1 (de) Bestimmung der Kupplungseinstellung
ID20438A (id) Pencetakan bahan serat
DE69715054T2 (de) Herstellung von Silanen
DE69813680D1 (de) Gruppenantenne unter Verwendung von Verzögerungsleitungen
TR199600931A2 (tr) Insaat malzemesi karisimi.
ID22437A (id) Unit bilah pengaduk
DE69809433D1 (de) Herstellung von avermectin-verbindungen
ATE250665T1 (de) Herstellung von zuckerrohr
DE59804303D1 (de) Herstellung von Polyenaldehyden
DE69632974D1 (de) DNS-Basen-Sequenzer
DE69811864D1 (de) Herstellung von bandförmigen werkstücken
DE69809562D1 (de) Herstellung von verbrückte hafnocene
DE29509921U1 (de) Holzbaukonstruktion zur Errichtung von Gebäuden

Legal Events

Date Code Title Description
8364 No opposition during term of opposition