DE69606180D1 - Verfahren zur detektion einer nukleinsaeure mittels einer nukleinsaeuresonde, das ein spezifisches einfangen und eine detektion auf einmal erlaubt - Google Patents

Verfahren zur detektion einer nukleinsaeure mittels einer nukleinsaeuresonde, das ein spezifisches einfangen und eine detektion auf einmal erlaubt

Info

Publication number
DE69606180D1
DE69606180D1 DE69606180T DE69606180T DE69606180D1 DE 69606180 D1 DE69606180 D1 DE 69606180D1 DE 69606180 T DE69606180 T DE 69606180T DE 69606180 T DE69606180 T DE 69606180T DE 69606180 D1 DE69606180 D1 DE 69606180D1
Authority
DE
Germany
Prior art keywords
nucleic acid
probe
detecting
allows detection
detection once
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69606180T
Other languages
English (en)
Other versions
DE69606180T2 (de
Inventor
Marta Blumenfeld
Michel Bouillot
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Biodevelopment SAS
Original Assignee
Serono Genetics Institute SA
Genset SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Serono Genetics Institute SA, Genset SA filed Critical Serono Genetics Institute SA
Application granted granted Critical
Publication of DE69606180D1 publication Critical patent/DE69606180D1/de
Publication of DE69606180T2 publication Critical patent/DE69606180T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
DE69606180T 1995-07-31 1996-07-30 Verfahren zur detektion einer nukleinsaeure mittels einer nukleinsaeuresonde, das ein spezifisches einfangen und eine detektion auf einmal erlaubt Expired - Fee Related DE69606180T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9509290A FR2737502B1 (fr) 1995-07-31 1995-07-31 Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection
PCT/FR1996/001201 WO1997005277A1 (fr) 1995-07-31 1996-07-30 Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection

Publications (2)

Publication Number Publication Date
DE69606180D1 true DE69606180D1 (de) 2000-02-17
DE69606180T2 DE69606180T2 (de) 2000-09-28

Family

ID=9481556

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69606180T Expired - Fee Related DE69606180T2 (de) 1995-07-31 1996-07-30 Verfahren zur detektion einer nukleinsaeure mittels einer nukleinsaeuresonde, das ein spezifisches einfangen und eine detektion auf einmal erlaubt

Country Status (9)

Country Link
US (1) US6228580B1 (de)
EP (1) EP0843738B1 (de)
JP (1) JP4050789B2 (de)
AT (1) ATE188745T1 (de)
AU (1) AU717454B2 (de)
CA (1) CA2228712A1 (de)
DE (1) DE69606180T2 (de)
FR (1) FR2737502B1 (de)
WO (1) WO1997005277A1 (de)

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WO2002091858A1 (en) * 2001-05-11 2002-11-21 University Of Ottawa Anxiolytic marcgraviaceae compositions containing betulinic acid, betulinic acid derivatives, and methods
US7083920B2 (en) * 2001-05-18 2006-08-01 Nagaoka & Co. Ltd. Surface assembly for immobilizing DNA capture probes in genetic assays using enzymatic reactions to generate signal in optical bio-discs and methods relating thereto
US7189509B2 (en) * 2001-08-16 2007-03-13 Zhifeng Shao Analysis of gene expression profiles using sequential hybridization
KR20030049802A (ko) * 2001-12-17 2003-06-25 권윤정 바이오 칩에서 프로브와 시료 핵산의 혼성화 여부를 효소반응으로 구별하는 방법
US20030175709A1 (en) * 2001-12-20 2003-09-18 Murphy George L. Method and system for depleting rRNA populations
US20050019901A1 (en) * 2002-01-31 2005-01-27 Evgenia Matveeva Methods for synthesis of bio-active nanoparticles and nanocapsules for use in optical bio-disc assays and disc assembly including same
KR20020041344A (ko) * 2002-02-26 2002-06-01 김희태 멤브레인 크로마토그래프 기법을 활용하여핵산중합반응으로 증폭된 핵산을 검출하는 방법
KR100492393B1 (ko) * 2002-12-02 2005-05-30 굿젠 주식회사 멤브레인 크로마토그래프 기법에 의한 핵산 검출 방법 및이를 위한 킷트
US20040265883A1 (en) * 2003-06-27 2004-12-30 Biocept, Inc. mRNA expression analysis
US7294462B2 (en) * 2004-03-17 2007-11-13 Japan Software Management Co., Ltd. Method for detection of base sequence of interest
EP1580280A1 (de) * 2004-03-23 2005-09-28 Japan Software Management Co., Ltd. Methode zur Detektion von Basensequenzen.
ATE452208T1 (de) * 2004-10-08 2010-01-15 Sysmex Corp Verfahren zum nachweis einer zielsubstanz unter verwendung einer nukleinsäuresonde
WO2006110314A2 (en) 2005-03-25 2006-10-19 Ambion, Inc. Methods and compositions for depleting abundant rna transcripts
JP5409005B2 (ja) * 2005-10-27 2014-02-05 ロゼッタ インファーマティックス エルエルシー 非ランダムプライマーを用いる核酸増幅
US8137936B2 (en) * 2005-11-29 2012-03-20 Macevicz Stephen C Selected amplification of polynucleotides
DE602006018352D1 (de) 2005-12-06 2010-12-30 Ambion Inc Rückübertragungs-primer und verfahren zu deren entwurf
JP2009268665A (ja) * 2008-05-07 2009-11-19 Canon Inc 吸入装置
US8288520B2 (en) * 2008-10-27 2012-10-16 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and system
ES2644516T3 (es) * 2009-01-28 2017-11-29 Qiagen Gaithersburg, Inc. Método y ensayo de preparación de muestras de gran volumen específico de secuencia
US9797000B2 (en) 2009-05-01 2017-10-24 Qiagen Gaithersburg Inc. Non-target amplification method for detection of RNA splice-forms in a sample
EP2478087B1 (de) 2009-09-14 2017-01-18 QIAGEN Gaithersburg, Inc. Zusammensetzungen und verfahren zur rückgewinnung von nukleinsäuren oder proteinen aus in zytologischen medien fixierten gewebeproben
CN102822189B (zh) 2010-01-29 2016-06-29 奇亚根盖瑟斯堡股份有限公司 用于核酸的序列特异性纯化和多重复合体分析(multiplex analysis)的方法和组合物
JP2013517803A (ja) 2010-01-29 2013-05-20 キアジェン ゲイサーズバーグ インコーポレイテッド サンプル中のｈｐｖの存在を決定および確認する方法
JP2013528049A (ja) 2010-05-19 2013-07-08 キアゲン ガイサーズバーグ アイエヌシー． 核酸の配列特異的な精製及び多重分析のための方法及び組成物
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US9512469B2 (en) * 2011-09-28 2016-12-06 Htg Molecular Diagnostics, Inc. Methods of co-detecting MRNA and small non-coding RNA
CN214973877U (zh) 2019-08-09 2021-12-03 胡桃钳医疗公司 用于形成治疗性多核苷酸的微流体设备及微流体路径装置

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Also Published As

Publication number Publication date
JPH11514850A (ja) 1999-12-21
AU6704596A (en) 1997-02-26
CA2228712A1 (fr) 1997-02-13
FR2737502B1 (fr) 1997-10-24
DE69606180T2 (de) 2000-09-28
FR2737502A1 (fr) 1997-02-07
WO1997005277A1 (fr) 1997-02-13
JP4050789B2 (ja) 2008-02-20
US6228580B1 (en) 2001-05-08
EP0843738A1 (de) 1998-05-27
ATE188745T1 (de) 2000-01-15
AU717454B2 (en) 2000-03-23
EP0843738B1 (de) 2000-01-12

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Legal Events

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8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee